Detection of serum anti-lipopolysaccharides (lps) antibodies from enterohemorrhagic e. coli (ehec) in asymptomatic kindergarten teachers of buenos aires province

Autores
Fernández Brando, Romina Jimena; Sacerdoti, Flavia; Amaral, María Marta; Bernal, Alan Mauro; Da Rocha, Marcelo; Belardo, Marcela; Palermo, Marina; Ibarra, Cristina
Año de publicación
2020
Idioma
inglés
Tipo de recurso
documento de conferencia
Estado
versión publicada
Descripción
EHEC is responsible for developing typical Hemolytic Uremic Syndrome (HUS) and Argentina has the highest incidence in the world. The endemo-epidemic behavior that HUS presents in our countrywas associated with person-to-person transmission. Since children below 5 years old are the most affected, our objective was to determine the frequency of kindergarten teachers with anti-LPS-EHECantibodies in serum. One hundred and fifty teachers from J.C. Paz district from Buenos Aires Province participated in an informative talk given by LuSUH, a non-governmental association, about good practices to reduce HUS transmission. Then, 63 of them voluntarily gave, under a signedwritten informed consent, a sample of blood obtained by finger puncture. A commercial ELISA (Chemtest, Arg) was used to detect specific IgM and IgG isotypes against 4 types of LPS-EHEC: O157, O145, O121 and O103. These variants represent approximately 87% of the EHEC serotypes associated with HUS in Argentina. The IgM finding was considered as indirect evidence of current infection with the EHEC specific serotype. Forty-two samples were evaluated, 60% (25/42) of them were positive for at least one type of LPS. Fifty-six percent of them (14/25) were IgM positive. Fifty percent of the positive samples were reactive for LPS O157, 26% for LPS O121, 12% for LPS O145 and 2% for LPS O103. Among the samples positive for LPS O157, 5% belonged to IgM isotype, 31% to IgG and 14% had both. The IgG isotype was the unique detected for LPS O145 and O103 (12 and2%, respectively). For LPS O121, the IgM isotype was detected in 2%, IgG in 12% and both isotypes in 12% of the samples. The elevated frequency of LPS-EHEC reactive samples (60%) reflects the high circulation of EHEC strains in our country. This agrees with the prevalence of anti-Stx2 antibodies reported in our population previously. Very strikingly, nearly half of these individuals carry IgM, suggesting that they would be in the active phase of infection.
Fil: Fernández Brando, Romina Jimena. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Medicina Experimental. Academia Nacional de Medicina de Buenos Aires. Instituto de Medicina Experimental; Argentina
Fil: Sacerdoti, Flavia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Fisiología y Biofísica Bernardo Houssay. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Fisiología y Biofísica Bernardo Houssay; Argentina
Fil: Amaral, María Marta. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Fisiología y Biofísica Bernardo Houssay. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Fisiología y Biofísica Bernardo Houssay; Argentina
Fil: Bernal, Alan Mauro. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Medicina Experimental. Academia Nacional de Medicina de Buenos Aires. Instituto de Medicina Experimental; Argentina
Fil: Da Rocha, Marcelo. No especifíca;
Fil: Belardo, Marcela. Universidad Nacional de Jose Clemente Paz; Argentina
Fil: Palermo, Marina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Medicina Experimental. Academia Nacional de Medicina de Buenos Aires. Instituto de Medicina Experimental; Argentina
Fil: Ibarra, Cristina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Fisiología y Biofísica Bernardo Houssay. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Fisiología y Biofísica Bernardo Houssay; Argentina
LXV Reunión Anual de la Sociedad Argentina de Investigación Clínica; LXVIII Reunión anual de la Sociedad Argentina de Inmunología y Reunión anual de la Sociedad Argentina de Fisiología
Argentina
Sociedad Argentina de Investigación Clínica
Sociedad Argentina de Inmunología
Sociedad Argentina De Fisiología
Materia
Inmunoglobulinas
TOXINA SHIGA
Escherichia coli productor de toxina shiga
prevalencia
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/189157
Acceder
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network_name_str CONICET Digital (CONICET)
spelling Detection of serum anti-lipopolysaccharides (lps) antibodies from enterohemorrhagic e. coli (ehec) in asymptomatic kindergarten teachers of buenos aires provinceFernández Brando, Romina JimenaSacerdoti, FlaviaAmaral, María MartaBernal, Alan MauroDa Rocha, MarceloBelardo, MarcelaPalermo, MarinaIbarra, CristinaInmunoglobulinasTOXINA SHIGAEscherichia coli productor de toxina shigaprevalenciahttps://purl.org/becyt/ford/3.3https://purl.org/becyt/ford/3EHEC is responsible for developing typical Hemolytic Uremic Syndrome (HUS) and Argentina has the highest incidence in the world. The endemo-epidemic behavior that HUS presents in our countrywas associated with person-to-person transmission. Since children below 5 years old are the most affected, our objective was to determine the frequency of kindergarten teachers with anti-LPS-EHECantibodies in serum. One hundred and fifty teachers from J.C. Paz district from Buenos Aires Province participated in an informative talk given by LuSUH, a non-governmental association, about good practices to reduce HUS transmission. Then, 63 of them voluntarily gave, under a signedwritten informed consent, a sample of blood obtained by finger puncture. A commercial ELISA (Chemtest, Arg) was used to detect specific IgM and IgG isotypes against 4 types of LPS-EHEC: O157, O145, O121 and O103. These variants represent approximately 87% of the EHEC serotypes associated with HUS in Argentina. The IgM finding was considered as indirect evidence of current infection with the EHEC specific serotype. Forty-two samples were evaluated, 60% (25/42) of them were positive for at least one type of LPS. Fifty-six percent of them (14/25) were IgM positive. Fifty percent of the positive samples were reactive for LPS O157, 26% for LPS O121, 12% for LPS O145 and 2% for LPS O103. Among the samples positive for LPS O157, 5% belonged to IgM isotype, 31% to IgG and 14% had both. The IgG isotype was the unique detected for LPS O145 and O103 (12 and2%, respectively). For LPS O121, the IgM isotype was detected in 2%, IgG in 12% and both isotypes in 12% of the samples. The elevated frequency of LPS-EHEC reactive samples (60%) reflects the high circulation of EHEC strains in our country. This agrees with the prevalence of anti-Stx2 antibodies reported in our population previously. Very strikingly, nearly half of these individuals carry IgM, suggesting that they would be in the active phase of infection.Fil: Fernández Brando, Romina Jimena. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Medicina Experimental. Academia Nacional de Medicina de Buenos Aires. Instituto de Medicina Experimental; ArgentinaFil: Sacerdoti, Flavia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Fisiología y Biofísica Bernardo Houssay. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Fisiología y Biofísica Bernardo Houssay; ArgentinaFil: Amaral, María Marta. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Fisiología y Biofísica Bernardo Houssay. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Fisiología y Biofísica Bernardo Houssay; ArgentinaFil: Bernal, Alan Mauro. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Medicina Experimental. Academia Nacional de Medicina de Buenos Aires. Instituto de Medicina Experimental; ArgentinaFil: Da Rocha, Marcelo. No especifíca;Fil: Belardo, Marcela. Universidad Nacional de Jose Clemente Paz; ArgentinaFil: Palermo, Marina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Medicina Experimental. Academia Nacional de Medicina de Buenos Aires. Instituto de Medicina Experimental; ArgentinaFil: Ibarra, Cristina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Fisiología y Biofísica Bernardo Houssay. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Fisiología y Biofísica Bernardo Houssay; ArgentinaLXV Reunión Anual de la Sociedad Argentina de Investigación Clínica; LXVIII Reunión anual de la Sociedad Argentina de Inmunología y Reunión anual de la Sociedad Argentina de FisiologíaArgentinaSociedad Argentina de Investigación ClínicaSociedad Argentina de InmunologíaSociedad Argentina De FisiologíaFundación Revista Medicina2020info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectReuniónJournalhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/189157Detection of serum anti-lipopolysaccharides (lps) antibodies from enterohemorrhagic e. coli (ehec) in asymptomatic kindergarten teachers of buenos aires province; LXV Reunión Anual de la Sociedad Argentina de Investigación Clínica; LXVIII Reunión anual de la Sociedad Argentina de Inmunología y Reunión anual de la Sociedad Argentina de Fisiología; Argentina; 2020; 164-164CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://medicinabuenosaires.com/revistas/vol80-20/s5/Mv80s5.pdfNacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:54:23Zoai:ri.conicet.gov.ar:11336/189157instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:54:23.589CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Detection of serum anti-lipopolysaccharides (lps) antibodies from enterohemorrhagic e. coli (ehec) in asymptomatic kindergarten teachers of buenos aires province
title Detection of serum anti-lipopolysaccharides (lps) antibodies from enterohemorrhagic e. coli (ehec) in asymptomatic kindergarten teachers of buenos aires province
spellingShingle Detection of serum anti-lipopolysaccharides (lps) antibodies from enterohemorrhagic e. coli (ehec) in asymptomatic kindergarten teachers of buenos aires province
Fernández Brando, Romina Jimena
Inmunoglobulinas
TOXINA SHIGA
Escherichia coli productor de toxina shiga
prevalencia
title_short Detection of serum anti-lipopolysaccharides (lps) antibodies from enterohemorrhagic e. coli (ehec) in asymptomatic kindergarten teachers of buenos aires province
title_full Detection of serum anti-lipopolysaccharides (lps) antibodies from enterohemorrhagic e. coli (ehec) in asymptomatic kindergarten teachers of buenos aires province
title_fullStr Detection of serum anti-lipopolysaccharides (lps) antibodies from enterohemorrhagic e. coli (ehec) in asymptomatic kindergarten teachers of buenos aires province
title_full_unstemmed Detection of serum anti-lipopolysaccharides (lps) antibodies from enterohemorrhagic e. coli (ehec) in asymptomatic kindergarten teachers of buenos aires province
title_sort Detection of serum anti-lipopolysaccharides (lps) antibodies from enterohemorrhagic e. coli (ehec) in asymptomatic kindergarten teachers of buenos aires province
dc.creator.none.fl_str_mv Fernández Brando, Romina Jimena
Sacerdoti, Flavia
Amaral, María Marta
Bernal, Alan Mauro
Da Rocha, Marcelo
Belardo, Marcela
Palermo, Marina
Ibarra, Cristina
author Fernández Brando, Romina Jimena
author_facet Fernández Brando, Romina Jimena
Sacerdoti, Flavia
Amaral, María Marta
Bernal, Alan Mauro
Da Rocha, Marcelo
Belardo, Marcela
Palermo, Marina
Ibarra, Cristina
author_role author
author2 Sacerdoti, Flavia
Amaral, María Marta
Bernal, Alan Mauro
Da Rocha, Marcelo
Belardo, Marcela
Palermo, Marina
Ibarra, Cristina
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Inmunoglobulinas
TOXINA SHIGA
Escherichia coli productor de toxina shiga
prevalencia
topic Inmunoglobulinas
TOXINA SHIGA
Escherichia coli productor de toxina shiga
prevalencia
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.3
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv EHEC is responsible for developing typical Hemolytic Uremic Syndrome (HUS) and Argentina has the highest incidence in the world. The endemo-epidemic behavior that HUS presents in our countrywas associated with person-to-person transmission. Since children below 5 years old are the most affected, our objective was to determine the frequency of kindergarten teachers with anti-LPS-EHECantibodies in serum. One hundred and fifty teachers from J.C. Paz district from Buenos Aires Province participated in an informative talk given by LuSUH, a non-governmental association, about good practices to reduce HUS transmission. Then, 63 of them voluntarily gave, under a signedwritten informed consent, a sample of blood obtained by finger puncture. A commercial ELISA (Chemtest, Arg) was used to detect specific IgM and IgG isotypes against 4 types of LPS-EHEC: O157, O145, O121 and O103. These variants represent approximately 87% of the EHEC serotypes associated with HUS in Argentina. The IgM finding was considered as indirect evidence of current infection with the EHEC specific serotype. Forty-two samples were evaluated, 60% (25/42) of them were positive for at least one type of LPS. Fifty-six percent of them (14/25) were IgM positive. Fifty percent of the positive samples were reactive for LPS O157, 26% for LPS O121, 12% for LPS O145 and 2% for LPS O103. Among the samples positive for LPS O157, 5% belonged to IgM isotype, 31% to IgG and 14% had both. The IgG isotype was the unique detected for LPS O145 and O103 (12 and2%, respectively). For LPS O121, the IgM isotype was detected in 2%, IgG in 12% and both isotypes in 12% of the samples. The elevated frequency of LPS-EHEC reactive samples (60%) reflects the high circulation of EHEC strains in our country. This agrees with the prevalence of anti-Stx2 antibodies reported in our population previously. Very strikingly, nearly half of these individuals carry IgM, suggesting that they would be in the active phase of infection.
Fil: Fernández Brando, Romina Jimena. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Medicina Experimental. Academia Nacional de Medicina de Buenos Aires. Instituto de Medicina Experimental; Argentina
Fil: Sacerdoti, Flavia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Fisiología y Biofísica Bernardo Houssay. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Fisiología y Biofísica Bernardo Houssay; Argentina
Fil: Amaral, María Marta. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Fisiología y Biofísica Bernardo Houssay. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Fisiología y Biofísica Bernardo Houssay; Argentina
Fil: Bernal, Alan Mauro. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Medicina Experimental. Academia Nacional de Medicina de Buenos Aires. Instituto de Medicina Experimental; Argentina
Fil: Da Rocha, Marcelo. No especifíca;
Fil: Belardo, Marcela. Universidad Nacional de Jose Clemente Paz; Argentina
Fil: Palermo, Marina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Medicina Experimental. Academia Nacional de Medicina de Buenos Aires. Instituto de Medicina Experimental; Argentina
Fil: Ibarra, Cristina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Fisiología y Biofísica Bernardo Houssay. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Fisiología y Biofísica Bernardo Houssay; Argentina
LXV Reunión Anual de la Sociedad Argentina de Investigación Clínica; LXVIII Reunión anual de la Sociedad Argentina de Inmunología y Reunión anual de la Sociedad Argentina de Fisiología
Argentina
Sociedad Argentina de Investigación Clínica
Sociedad Argentina de Inmunología
Sociedad Argentina De Fisiología
description EHEC is responsible for developing typical Hemolytic Uremic Syndrome (HUS) and Argentina has the highest incidence in the world. The endemo-epidemic behavior that HUS presents in our countrywas associated with person-to-person transmission. Since children below 5 years old are the most affected, our objective was to determine the frequency of kindergarten teachers with anti-LPS-EHECantibodies in serum. One hundred and fifty teachers from J.C. Paz district from Buenos Aires Province participated in an informative talk given by LuSUH, a non-governmental association, about good practices to reduce HUS transmission. Then, 63 of them voluntarily gave, under a signedwritten informed consent, a sample of blood obtained by finger puncture. A commercial ELISA (Chemtest, Arg) was used to detect specific IgM and IgG isotypes against 4 types of LPS-EHEC: O157, O145, O121 and O103. These variants represent approximately 87% of the EHEC serotypes associated with HUS in Argentina. The IgM finding was considered as indirect evidence of current infection with the EHEC specific serotype. Forty-two samples were evaluated, 60% (25/42) of them were positive for at least one type of LPS. Fifty-six percent of them (14/25) were IgM positive. Fifty percent of the positive samples were reactive for LPS O157, 26% for LPS O121, 12% for LPS O145 and 2% for LPS O103. Among the samples positive for LPS O157, 5% belonged to IgM isotype, 31% to IgG and 14% had both. The IgG isotype was the unique detected for LPS O145 and O103 (12 and2%, respectively). For LPS O121, the IgM isotype was detected in 2%, IgG in 12% and both isotypes in 12% of the samples. The elevated frequency of LPS-EHEC reactive samples (60%) reflects the high circulation of EHEC strains in our country. This agrees with the prevalence of anti-Stx2 antibodies reported in our population previously. Very strikingly, nearly half of these individuals carry IgM, suggesting that they would be in the active phase of infection.
publishDate 2020
dc.date.none.fl_str_mv 2020
dc.type.none.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/conferenceObject
Reunión
Journal
http://purl.org/coar/resource_type/c_5794
info:ar-repo/semantics/documentoDeConferencia
status_str publishedVersion
format conferenceObject
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/189157
Detection of serum anti-lipopolysaccharides (lps) antibodies from enterohemorrhagic e. coli (ehec) in asymptomatic kindergarten teachers of buenos aires province; LXV Reunión Anual de la Sociedad Argentina de Investigación Clínica; LXVIII Reunión anual de la Sociedad Argentina de Inmunología y Reunión anual de la Sociedad Argentina de Fisiología; Argentina; 2020; 164-164
CONICET Digital
CONICET
url http://hdl.handle.net/11336/189157
identifier_str_mv Detection of serum anti-lipopolysaccharides (lps) antibodies from enterohemorrhagic e. coli (ehec) in asymptomatic kindergarten teachers of buenos aires province; LXV Reunión Anual de la Sociedad Argentina de Investigación Clínica; LXVIII Reunión anual de la Sociedad Argentina de Inmunología y Reunión anual de la Sociedad Argentina de Fisiología; Argentina; 2020; 164-164
CONICET Digital
CONICET
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language eng
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dc.coverage.none.fl_str_mv Nacional
dc.publisher.none.fl_str_mv Fundación Revista Medicina
publisher.none.fl_str_mv Fundación Revista Medicina
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repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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