Renal damage and death in weaned mice after oral infection with Shiga toxin 2-producing Escherichia coli strains

Autores
Brando, R J F; Miliwebsky, Elizabeth; Bentancor, L; Deza, N.; Baschkier, Ariela; Ramos, Marina A.; Fernandez, G. C.; Meiss, R; Rivas, Marta; Palermo, M.
Año de publicación
2008
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Fil: Brando, R J F. División Inmunología, Instituto de Investigationes Hematológicas, Academia Nacional de Medicina; Argentina.
Fil: Miliwebsky, Elizabeth. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Departamento de Bacteriología. Servicio Fisiopatogenia; Argentina.
Fil: Bentancor, L. División Inmunología, Instituto de Investigationes Hematológicas, Academia Nacional de Medicina; Argentina.
Fil: Deza, N. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Departamento de Bacteriología. Servicio Fisiopatogenia; Argentina.
Fil: Baschkier, Ariela. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Departamento de Bacteriología. Servicio Fisiopatogenia; Argentina.
Fil: Ramos, Marina A. División Inmunología, Instituto de Investigationes Hematológicas, Academia Nacional de Medicina; Argentina.
Fil: Fernández, G. C. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Departamento de Bacteriología. Servicio Fisiopatogenia; Argentina.
Fil: Meiss, R. Departamento de Patología, Centro de Estudios Oncológicos, Academia Nacional de Medicina; Argentina.
Fil: Rivas, Marta. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Departamento de Bacteriología. Servicio Fisiopatogenia; Argentina.
Fil: Palermo, M. División Inmunología, Instituto de Investigationes Hematológicas, Academia Nacional de Medicina; Argentina.
Enterohaemorrhagic Escherichia coli (EHEC) O157:H7 infections are considered a public health problem in both developed and developing countries because of their increasing incidence and the severity of clinical presentation. Approximately 10% of infected patients develop complications such as haemolytic uraemic syndrome (HUS) characterized by acute renal failure, thrombocytopenia and haemolytic anaemia. The precise sequence of events leading to HUS is still understood incompletely. Because of the lack of a reproducible small animal model for EHEC infections, in vivo studies examining EHEC-host early interactions are limited and insufficient. The aim of this study was to characterize the weaned BALB/c mouse as a model of E. coli O157:H7 infection. In this paper we report that human Shiga toxin 2 (Stx2)-producing EHEC strains can adhere to the intestinal epithelium of weaned BALB/c mice, and produce local damage which leads to systemic disease and death in a percentage of infected mice. The lethality of the EHEC strain is closely age-dependent, and is related to the bacterial ability to colonize intestine and to produce Stx2. It can be concluded that the weaned BALB/c mouse can be used as a small animal model to study host early responses, and the role of bacterial pathogenic factors in the induction of systemic disease, thus providing a useful tool for the evaluation of therapeutic or vaccine approaches.
Fuente
Clinical and experimental immunology 2008;153(2):297-306.
Materia
Escherichia coli
Toxina Shiga
Nivel de accesibilidad
acceso abierto
Condiciones de uso
Repositorio
Sistema de Gestión del Conocimiento ANLIS MALBRÁN
Institución
Administración Nacional de Laboratorios e Institutos de Salud "Dr. Carlos G. Malbrán"
OAI Identificador
oai:sgc.anlis.gob.ar:123456789/1857
Acceder
id SGCANLIS_20c87ab7593d7377ca96eae896ff16a1
oai_identifier_str oai:sgc.anlis.gob.ar:123456789/1857
network_acronym_str SGCANLIS
repository_id_str a
network_name_str Sistema de Gestión del Conocimiento ANLIS MALBRÁN
spelling Renal damage and death in weaned mice after oral infection with Shiga toxin 2-producing Escherichia coli strainsBrando, R J FMiliwebsky, ElizabethBentancor, LDeza, N.Baschkier, ArielaRamos, Marina A.Fernandez, G. C.Meiss, RRivas, MartaPalermo, M.Escherichia coliToxina ShigaFil: Brando, R J F. División Inmunología, Instituto de Investigationes Hematológicas, Academia Nacional de Medicina; Argentina.Fil: Miliwebsky, Elizabeth. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Departamento de Bacteriología. Servicio Fisiopatogenia; Argentina.Fil: Bentancor, L. División Inmunología, Instituto de Investigationes Hematológicas, Academia Nacional de Medicina; Argentina.Fil: Deza, N. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Departamento de Bacteriología. Servicio Fisiopatogenia; Argentina.Fil: Baschkier, Ariela. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Departamento de Bacteriología. Servicio Fisiopatogenia; Argentina.Fil: Ramos, Marina A. División Inmunología, Instituto de Investigationes Hematológicas, Academia Nacional de Medicina; Argentina.Fil: Fernández, G. C. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Departamento de Bacteriología. Servicio Fisiopatogenia; Argentina.Fil: Meiss, R. Departamento de Patología, Centro de Estudios Oncológicos, Academia Nacional de Medicina; Argentina.Fil: Rivas, Marta. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Departamento de Bacteriología. Servicio Fisiopatogenia; Argentina.Fil: Palermo, M. División Inmunología, Instituto de Investigationes Hematológicas, Academia Nacional de Medicina; Argentina.Enterohaemorrhagic Escherichia coli (EHEC) O157:H7 infections are considered a public health problem in both developed and developing countries because of their increasing incidence and the severity of clinical presentation. Approximately 10% of infected patients develop complications such as haemolytic uraemic syndrome (HUS) characterized by acute renal failure, thrombocytopenia and haemolytic anaemia. The precise sequence of events leading to HUS is still understood incompletely. Because of the lack of a reproducible small animal model for EHEC infections, in vivo studies examining EHEC-host early interactions are limited and insufficient. The aim of this study was to characterize the weaned BALB/c mouse as a model of E. coli O157:H7 infection. In this paper we report that human Shiga toxin 2 (Stx2)-producing EHEC strains can adhere to the intestinal epithelium of weaned BALB/c mice, and produce local damage which leads to systemic disease and death in a percentage of infected mice. The lethality of the EHEC strain is closely age-dependent, and is related to the bacterial ability to colonize intestine and to produce Stx2. It can be concluded that the weaned BALB/c mouse can be used as a small animal model to study host early responses, and the role of bacterial pathogenic factors in the induction of systemic disease, thus providing a useful tool for the evaluation of therapeutic or vaccine approaches.Wiley2008-08info:ar-repo/semantics/articuloinfo:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdf1365-2249http://sgc.anlis.gob.ar/handle/123456789/185710.1111/j.1365-2249.2008.03698.xClinical and experimental immunology 2008;153(2):297-306.reponame:Sistema de Gestión del Conocimiento ANLIS MALBRÁNinstname:Administración Nacional de Laboratorios e Institutos de Salud "Dr. Carlos G. Malbrán"instacron:ANLISClinical and experimental immunologyenginfo:eu-repo/semantics/openAccess2025-09-04T11:17:44Zoai:sgc.anlis.gob.ar:123456789/1857Institucionalhttp://sgc.anlis.gob.ar/Organismo científico-tecnológicoNo correspondehttp://sgc.anlis.gob.ar/oai/biblioteca@anlis.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:a2025-09-04 11:17:44.641Sistema de Gestión del Conocimiento ANLIS MALBRÁN - Administración Nacional de Laboratorios e Institutos de Salud "Dr. Carlos G. Malbrán"false
dc.title.none.fl_str_mv Renal damage and death in weaned mice after oral infection with Shiga toxin 2-producing Escherichia coli strains
title Renal damage and death in weaned mice after oral infection with Shiga toxin 2-producing Escherichia coli strains
spellingShingle Renal damage and death in weaned mice after oral infection with Shiga toxin 2-producing Escherichia coli strains
Brando, R J F
Escherichia coli
Toxina Shiga
title_short Renal damage and death in weaned mice after oral infection with Shiga toxin 2-producing Escherichia coli strains
title_full Renal damage and death in weaned mice after oral infection with Shiga toxin 2-producing Escherichia coli strains
title_fullStr Renal damage and death in weaned mice after oral infection with Shiga toxin 2-producing Escherichia coli strains
title_full_unstemmed Renal damage and death in weaned mice after oral infection with Shiga toxin 2-producing Escherichia coli strains
title_sort Renal damage and death in weaned mice after oral infection with Shiga toxin 2-producing Escherichia coli strains
dc.creator.none.fl_str_mv Brando, R J F
Miliwebsky, Elizabeth
Bentancor, L
Deza, N.
Baschkier, Ariela
Ramos, Marina A.
Fernandez, G. C.
Meiss, R
Rivas, Marta
Palermo, M.
author Brando, R J F
author_facet Brando, R J F
Miliwebsky, Elizabeth
Bentancor, L
Deza, N.
Baschkier, Ariela
Ramos, Marina A.
Fernandez, G. C.
Meiss, R
Rivas, Marta
Palermo, M.
author_role author
author2 Miliwebsky, Elizabeth
Bentancor, L
Deza, N.
Baschkier, Ariela
Ramos, Marina A.
Fernandez, G. C.
Meiss, R
Rivas, Marta
Palermo, M.
author2_role author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Escherichia coli
Toxina Shiga
topic Escherichia coli
Toxina Shiga
dc.description.none.fl_txt_mv Fil: Brando, R J F. División Inmunología, Instituto de Investigationes Hematológicas, Academia Nacional de Medicina; Argentina.
Fil: Miliwebsky, Elizabeth. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Departamento de Bacteriología. Servicio Fisiopatogenia; Argentina.
Fil: Bentancor, L. División Inmunología, Instituto de Investigationes Hematológicas, Academia Nacional de Medicina; Argentina.
Fil: Deza, N. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Departamento de Bacteriología. Servicio Fisiopatogenia; Argentina.
Fil: Baschkier, Ariela. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Departamento de Bacteriología. Servicio Fisiopatogenia; Argentina.
Fil: Ramos, Marina A. División Inmunología, Instituto de Investigationes Hematológicas, Academia Nacional de Medicina; Argentina.
Fil: Fernández, G. C. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Departamento de Bacteriología. Servicio Fisiopatogenia; Argentina.
Fil: Meiss, R. Departamento de Patología, Centro de Estudios Oncológicos, Academia Nacional de Medicina; Argentina.
Fil: Rivas, Marta. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Departamento de Bacteriología. Servicio Fisiopatogenia; Argentina.
Fil: Palermo, M. División Inmunología, Instituto de Investigationes Hematológicas, Academia Nacional de Medicina; Argentina.
Enterohaemorrhagic Escherichia coli (EHEC) O157:H7 infections are considered a public health problem in both developed and developing countries because of their increasing incidence and the severity of clinical presentation. Approximately 10% of infected patients develop complications such as haemolytic uraemic syndrome (HUS) characterized by acute renal failure, thrombocytopenia and haemolytic anaemia. The precise sequence of events leading to HUS is still understood incompletely. Because of the lack of a reproducible small animal model for EHEC infections, in vivo studies examining EHEC-host early interactions are limited and insufficient. The aim of this study was to characterize the weaned BALB/c mouse as a model of E. coli O157:H7 infection. In this paper we report that human Shiga toxin 2 (Stx2)-producing EHEC strains can adhere to the intestinal epithelium of weaned BALB/c mice, and produce local damage which leads to systemic disease and death in a percentage of infected mice. The lethality of the EHEC strain is closely age-dependent, and is related to the bacterial ability to colonize intestine and to produce Stx2. It can be concluded that the weaned BALB/c mouse can be used as a small animal model to study host early responses, and the role of bacterial pathogenic factors in the induction of systemic disease, thus providing a useful tool for the evaluation of therapeutic or vaccine approaches.
description Fil: Brando, R J F. División Inmunología, Instituto de Investigationes Hematológicas, Academia Nacional de Medicina; Argentina.
publishDate 2008
dc.date.none.fl_str_mv 2008-08
dc.type.none.fl_str_mv info:ar-repo/semantics/articulo
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv 1365-2249
http://sgc.anlis.gob.ar/handle/123456789/1857
10.1111/j.1365-2249.2008.03698.x
identifier_str_mv 1365-2249
10.1111/j.1365-2249.2008.03698.x
url http://sgc.anlis.gob.ar/handle/123456789/1857
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Clinical and experimental immunology
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Wiley
publisher.none.fl_str_mv Wiley
dc.source.none.fl_str_mv Clinical and experimental immunology 2008;153(2):297-306.
reponame:Sistema de Gestión del Conocimiento ANLIS MALBRÁN
instname:Administración Nacional de Laboratorios e Institutos de Salud "Dr. Carlos G. Malbrán"
instacron:ANLIS
reponame_str Sistema de Gestión del Conocimiento ANLIS MALBRÁN
collection Sistema de Gestión del Conocimiento ANLIS MALBRÁN
instname_str Administración Nacional de Laboratorios e Institutos de Salud "Dr. Carlos G. Malbrán"
instacron_str ANLIS
institution ANLIS
repository.name.fl_str_mv Sistema de Gestión del Conocimiento ANLIS MALBRÁN - Administración Nacional de Laboratorios e Institutos de Salud "Dr. Carlos G. Malbrán"
repository.mail.fl_str_mv biblioteca@anlis.gov.ar
_version_ 1842344422446989312
score 12.623145

Publicaciones similares