Evaluation of the sensitivity and specificity of bovine tuberculosis diagnostic tests in naturally infected cattle herds using a Bayesian approach

Autores
Álvarez, Julio; Perez, Andres Maximiliano; Bezos, Javier; Marqués, Sergio; Grau, Anna; Saez, Jose Luis; Mínguez, Olga; de Juan, Lucía; Domínguez, Lucas
Año de publicación
2012
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Test-and-slaughter strategies have been the basis of bovine tuberculosis (BT) eradication programs worldwide; however, eradication efforts have not succeeded in certain regions, and imperfect sensitivity and specificity of applied diagnostic techniques have been deemed as one of the possible causes for such failure. Evaluation of tuberculosis diagnostic tools has been impaired by the lack of an adequate gold standard to define positive and negative individuals. Here, a Bayesian approach was formulated to estimate for the first time sensitivity (Se) and specificity (Sp) of the tests [single intradermal tuberculin (SIT) test, and interferon-gamma (IFN-g) assay] currently used in Spain. Field data from the first implementation of IFN-g assay (used in parallel with SIT test 2–6 months after a first disclosure SIT test) in infected beef, dairy and bullfighting cattle herds from the region of Castilla and Leon were used for the analysis. Model results suggested that in the described situation: (i) Se of SIT test was highly variable (40.1–92.2% for severe interpretation, median = 66–69%), and its Sp was high (>99%) regardless interpretation criteria; (ii) IFN-g assay showed a high Se (median = 89–90% and 83.5% for 0.05 and 0.1 cut-off points respectively) and an acceptable Sp (85.7% and 90.3% for 0.05 and 0.1 thresholds) and (iii) parallel application of both tests maximized the combined Se (95.6% using severe SIT and 0.05 cut-off point in the IFN-g assay). These results support the potential use of the IFN-g assay as an ancillary technique for routine BT diagnosis.
Fil: Álvarez, Julio. Consejo Superior de Investigaciones Científicas; España
Fil: Perez, Andres Maximiliano. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario; Argentina. Universidad Nacional de Rosario; Argentina
Fil: Bezos, Javier. Universidad Complutense de Madrid; España
Fil: Marqués, Sergio. No especifíca;
Fil: Grau, Anna. No especifíca;
Fil: Saez, Jose Luis. No especifíca;
Fil: Mínguez, Olga. No especifíca;
Fil: de Juan, Lucía. Universidad Complutense de Madrid; España
Fil: Domínguez, Lucas. Universidad Complutense de Madrid; España
Materia
tuberculosis
cattle
diagnostic tests
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/268737
Acceder
id CONICETDig_6c5412bb4086e535596f1498150e5a42
oai_identifier_str oai:ri.conicet.gov.ar:11336/268737
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Evaluation of the sensitivity and specificity of bovine tuberculosis diagnostic tests in naturally infected cattle herds using a Bayesian approachÁlvarez, JulioPerez, Andres MaximilianoBezos, JavierMarqués, SergioGrau, AnnaSaez, Jose LuisMínguez, Olgade Juan, LucíaDomínguez, Lucastuberculosiscattlediagnostic testshttps://purl.org/becyt/ford/4.3https://purl.org/becyt/ford/4Test-and-slaughter strategies have been the basis of bovine tuberculosis (BT) eradication programs worldwide; however, eradication efforts have not succeeded in certain regions, and imperfect sensitivity and specificity of applied diagnostic techniques have been deemed as one of the possible causes for such failure. Evaluation of tuberculosis diagnostic tools has been impaired by the lack of an adequate gold standard to define positive and negative individuals. Here, a Bayesian approach was formulated to estimate for the first time sensitivity (Se) and specificity (Sp) of the tests [single intradermal tuberculin (SIT) test, and interferon-gamma (IFN-g) assay] currently used in Spain. Field data from the first implementation of IFN-g assay (used in parallel with SIT test 2–6 months after a first disclosure SIT test) in infected beef, dairy and bullfighting cattle herds from the region of Castilla and Leon were used for the analysis. Model results suggested that in the described situation: (i) Se of SIT test was highly variable (40.1–92.2% for severe interpretation, median = 66–69%), and its Sp was high (>99%) regardless interpretation criteria; (ii) IFN-g assay showed a high Se (median = 89–90% and 83.5% for 0.05 and 0.1 cut-off points respectively) and an acceptable Sp (85.7% and 90.3% for 0.05 and 0.1 thresholds) and (iii) parallel application of both tests maximized the combined Se (95.6% using severe SIT and 0.05 cut-off point in the IFN-g assay). These results support the potential use of the IFN-g assay as an ancillary technique for routine BT diagnosis.Fil: Álvarez, Julio. Consejo Superior de Investigaciones Científicas; EspañaFil: Perez, Andres Maximiliano. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario; Argentina. Universidad Nacional de Rosario; ArgentinaFil: Bezos, Javier. Universidad Complutense de Madrid; EspañaFil: Marqués, Sergio. No especifíca;Fil: Grau, Anna. No especifíca;Fil: Saez, Jose Luis. No especifíca;Fil: Mínguez, Olga. No especifíca;Fil: de Juan, Lucía. Universidad Complutense de Madrid; EspañaFil: Domínguez, Lucas. Universidad Complutense de Madrid; EspañaElsevier Science2012-02info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/268737Álvarez, Julio; Perez, Andres Maximiliano; Bezos, Javier; Marqués, Sergio; Grau, Anna; et al.; Evaluation of the sensitivity and specificity of bovine tuberculosis diagnostic tests in naturally infected cattle herds using a Bayesian approach; Elsevier Science; Veterinary Microbiology; 155; 1; 2-2012; 38-430378-1135CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/abs/pii/S0378113511004305info:eu-repo/semantics/altIdentifier/doi/10.1016/j.vetmic.2011.07.034info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-22T11:05:26Zoai:ri.conicet.gov.ar:11336/268737instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-22 11:05:27.212CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Evaluation of the sensitivity and specificity of bovine tuberculosis diagnostic tests in naturally infected cattle herds using a Bayesian approach
title Evaluation of the sensitivity and specificity of bovine tuberculosis diagnostic tests in naturally infected cattle herds using a Bayesian approach
spellingShingle Evaluation of the sensitivity and specificity of bovine tuberculosis diagnostic tests in naturally infected cattle herds using a Bayesian approach
Álvarez, Julio
tuberculosis
cattle
diagnostic tests
title_short Evaluation of the sensitivity and specificity of bovine tuberculosis diagnostic tests in naturally infected cattle herds using a Bayesian approach
title_full Evaluation of the sensitivity and specificity of bovine tuberculosis diagnostic tests in naturally infected cattle herds using a Bayesian approach
title_fullStr Evaluation of the sensitivity and specificity of bovine tuberculosis diagnostic tests in naturally infected cattle herds using a Bayesian approach
title_full_unstemmed Evaluation of the sensitivity and specificity of bovine tuberculosis diagnostic tests in naturally infected cattle herds using a Bayesian approach
title_sort Evaluation of the sensitivity and specificity of bovine tuberculosis diagnostic tests in naturally infected cattle herds using a Bayesian approach
dc.creator.none.fl_str_mv Álvarez, Julio
Perez, Andres Maximiliano
Bezos, Javier
Marqués, Sergio
Grau, Anna
Saez, Jose Luis
Mínguez, Olga
de Juan, Lucía
Domínguez, Lucas
author Álvarez, Julio
author_facet Álvarez, Julio
Perez, Andres Maximiliano
Bezos, Javier
Marqués, Sergio
Grau, Anna
Saez, Jose Luis
Mínguez, Olga
de Juan, Lucía
Domínguez, Lucas
author_role author
author2 Perez, Andres Maximiliano
Bezos, Javier
Marqués, Sergio
Grau, Anna
Saez, Jose Luis
Mínguez, Olga
de Juan, Lucía
Domínguez, Lucas
author2_role author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv tuberculosis
cattle
diagnostic tests
topic tuberculosis
cattle
diagnostic tests
purl_subject.fl_str_mv https://purl.org/becyt/ford/4.3
https://purl.org/becyt/ford/4
dc.description.none.fl_txt_mv Test-and-slaughter strategies have been the basis of bovine tuberculosis (BT) eradication programs worldwide; however, eradication efforts have not succeeded in certain regions, and imperfect sensitivity and specificity of applied diagnostic techniques have been deemed as one of the possible causes for such failure. Evaluation of tuberculosis diagnostic tools has been impaired by the lack of an adequate gold standard to define positive and negative individuals. Here, a Bayesian approach was formulated to estimate for the first time sensitivity (Se) and specificity (Sp) of the tests [single intradermal tuberculin (SIT) test, and interferon-gamma (IFN-g) assay] currently used in Spain. Field data from the first implementation of IFN-g assay (used in parallel with SIT test 2–6 months after a first disclosure SIT test) in infected beef, dairy and bullfighting cattle herds from the region of Castilla and Leon were used for the analysis. Model results suggested that in the described situation: (i) Se of SIT test was highly variable (40.1–92.2% for severe interpretation, median = 66–69%), and its Sp was high (>99%) regardless interpretation criteria; (ii) IFN-g assay showed a high Se (median = 89–90% and 83.5% for 0.05 and 0.1 cut-off points respectively) and an acceptable Sp (85.7% and 90.3% for 0.05 and 0.1 thresholds) and (iii) parallel application of both tests maximized the combined Se (95.6% using severe SIT and 0.05 cut-off point in the IFN-g assay). These results support the potential use of the IFN-g assay as an ancillary technique for routine BT diagnosis.
Fil: Álvarez, Julio. Consejo Superior de Investigaciones Científicas; España
Fil: Perez, Andres Maximiliano. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario; Argentina. Universidad Nacional de Rosario; Argentina
Fil: Bezos, Javier. Universidad Complutense de Madrid; España
Fil: Marqués, Sergio. No especifíca;
Fil: Grau, Anna. No especifíca;
Fil: Saez, Jose Luis. No especifíca;
Fil: Mínguez, Olga. No especifíca;
Fil: de Juan, Lucía. Universidad Complutense de Madrid; España
Fil: Domínguez, Lucas. Universidad Complutense de Madrid; España
description Test-and-slaughter strategies have been the basis of bovine tuberculosis (BT) eradication programs worldwide; however, eradication efforts have not succeeded in certain regions, and imperfect sensitivity and specificity of applied diagnostic techniques have been deemed as one of the possible causes for such failure. Evaluation of tuberculosis diagnostic tools has been impaired by the lack of an adequate gold standard to define positive and negative individuals. Here, a Bayesian approach was formulated to estimate for the first time sensitivity (Se) and specificity (Sp) of the tests [single intradermal tuberculin (SIT) test, and interferon-gamma (IFN-g) assay] currently used in Spain. Field data from the first implementation of IFN-g assay (used in parallel with SIT test 2–6 months after a first disclosure SIT test) in infected beef, dairy and bullfighting cattle herds from the region of Castilla and Leon were used for the analysis. Model results suggested that in the described situation: (i) Se of SIT test was highly variable (40.1–92.2% for severe interpretation, median = 66–69%), and its Sp was high (>99%) regardless interpretation criteria; (ii) IFN-g assay showed a high Se (median = 89–90% and 83.5% for 0.05 and 0.1 cut-off points respectively) and an acceptable Sp (85.7% and 90.3% for 0.05 and 0.1 thresholds) and (iii) parallel application of both tests maximized the combined Se (95.6% using severe SIT and 0.05 cut-off point in the IFN-g assay). These results support the potential use of the IFN-g assay as an ancillary technique for routine BT diagnosis.
publishDate 2012
dc.date.none.fl_str_mv 2012-02
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/268737
Álvarez, Julio; Perez, Andres Maximiliano; Bezos, Javier; Marqués, Sergio; Grau, Anna; et al.; Evaluation of the sensitivity and specificity of bovine tuberculosis diagnostic tests in naturally infected cattle herds using a Bayesian approach; Elsevier Science; Veterinary Microbiology; 155; 1; 2-2012; 38-43
0378-1135
CONICET Digital
CONICET
url http://hdl.handle.net/11336/268737
identifier_str_mv Álvarez, Julio; Perez, Andres Maximiliano; Bezos, Javier; Marqués, Sergio; Grau, Anna; et al.; Evaluation of the sensitivity and specificity of bovine tuberculosis diagnostic tests in naturally infected cattle herds using a Bayesian approach; Elsevier Science; Veterinary Microbiology; 155; 1; 2-2012; 38-43
0378-1135
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/abs/pii/S0378113511004305
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.vetmic.2011.07.034
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Science
publisher.none.fl_str_mv Elsevier Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 12.982451

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