Genome-Wide Expression Analysis in Drosophila Reveals Genes Controlling Circadian Behavior

• Autores: Ceriani, Maria Fernanda; Hogenesch, John B.; Yanovsky, Marcelo Javier; Panda, Satchidananda; Straume, Martin; Kay, Steve A.
• Año de publicación: 2002
• Idioma: inglés
• Tipo de recurso: artículo
• Materia: Genechip Affymetrix; Drosophila; slowpoke; arrthythmic behavior
• Institución: Consejo Nacional de Investigaciones Científicas y Técnicas

In Drosophila, a number of key processes such as emergence from the pupal case, locomotor activity, feeding, olfaction, and aspects of mating behavior are under circadian regulation. Although we have a basic understanding of how the molecular oscillations take place, a clear link between gene regulation and downstream biological processes is still missing. To identify clock-controlled output genes, we have used an oligonucleotide-based high-density array that interrogates gene expression changes on a whole genome level. We found genes regulating various physiological processes to be under circadian transcriptional regulation, ranging from protein stability and degradation, signal transduction, heme metabolism, detoxification, and immunity. By comparing rhythmically expressed genes in the fly head and body, we found that the clock has adapted its output functions to the needs of each particular tissue, implying that tissue-specific regulation is superimposed on clock control of gene expression. Finally, taking full advantage of the fly as a model system, we have identified and characterized a cycling potassium channel protein as a key step in linking the transcriptional feedback loop to rhythmic locomotor behavior.
Fil: Ceriani, Maria Fernanda. The Scripps Research Institute; Estados Unidos. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; Argentina
Fil: Hogenesch, John B.. Genomics Institute of the Novartis Research Foundation; Estados Unidos
Fil: Yanovsky, Marcelo Javier. The Scripps Research Institute; Estados Unidos. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; Argentina
Fil: Panda, Satchidananda. Genomics Institute of the Novartis Research Foundation; Estados Unidos. The Scripps Research Institute; Estados Unidos
Fil: Straume, Martin. University Of Virginia; Estados Unidos
Fil: Kay, Steve A.. Genomics Institute of the Novartis Research Foundation; Estados Unidos. The Scripps Research Institute; Estados Unidos