Myoinhibiting peptide signaling via sex peptide receptor is critical for proper glue expulsion and spreading behavior during drosophila development

Autores
Fernandez Acosta, Magdalena; Heredia, Fabiana; Menezes, Juliane; Zanini, Rebeca; Prüger, Katja; Dronseck, Agustina; Arana, Maite; Pereirinha, Joana; Leal, Ana; Veenstra, Jan A.; Wegener, Christian; Gontijo, Alisson; Garelli, Andres
Año de publicación
2022
Idioma
inglés
Tipo de recurso
documento de conferencia
Estado
versión publicada
Descripción
Drosophila pupariation consists of a series of innate behaviors and morphogenetic changes that reduce predation and desiccation during metamorphosis via the formation of a compacted and hardened pupal case (puparium) from the larval cuticle and its firm attachment to a substrate. The latter is achieved by a rapid and highly-stereotyped behavior, glue expulsion and spreading behavior (GSB), which is preceded by strong body-remodelling contractions, termed pre-GSB. These behaviors must be executed in order and precede cuticle hardening to ensure proper pupariation. The steroid hormone ecdysone coordinates the whole pupariation process, inducing initiation of pre-GSB by an unknown mechanism and its progression to stronger contractions and ultimately to GSB by inducing a cuticle epidermis-to-CNS neuron relaxin-like Dilp8-Lgr3 signaling event. The factors, if any, that induce GSB and post-GSB behaviors downstream of ecdysone remain to be defined. Here we use neuronal-specific RNA interference against a series of neuropeptides to identify Myoinhibiting peptide (MIP) as a critical peptide required for proper GSB. Behavioral monitoring using a muscle calcium reporter (GCamp) shows that the execution of GSB is abnormal in the absence of MIP. Epistasis assays show that MIP acts spatially and temporally downstream of the Dilp8-Lgr3 pathway. Cell type-specific MIP RNAi showed that MIP is required in a single bilateral brain neuron for proper GSB. Further experiments show that MIP acts on neurons expressing Sex-peptide receptor (SPR), which is also critical for proper GSB. We propose that MIP is a key modulator of motor circuits during pupariation. Our results significantly advance our molecular and cellular understanding of pupariation control, and contributes to the understanding of how multi-step innate behaviors are coordinated in time
Fil: Fernandez Acosta, Magdalena. Universidade Nova de Lisboa. Faculdade de Ciencias Medicas; Portugal
Fil: Heredia, Fabiana. Universidade Nova de Lisboa. Faculdade de Ciencias Medicas; Portugal
Fil: Menezes, Juliane. Universidade Nova de Lisboa. Faculdade de Ciencias Medicas; Portugal
Fil: Zanini, Rebeca. Universidade Nova de Lisboa. Faculdade de Ciencias Medicas; Portugal
Fil: Prüger, Katja. Universidade Nova de Lisboa. Faculdade de Ciencias Medicas; Portugal
Fil: Dronseck, Agustina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Fil: Arana, Maite. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Fil: Pereirinha, Joana. Universidade Nova de Lisboa. Faculdade de Ciencias Medicas; Portugal
Fil: Leal, Ana. Universidade Nova de Lisboa. Faculdade de Ciencias Medicas; Portugal
Fil: Veenstra, Jan A.. Universidade Nova de Lisboa. Faculdade de Ciencias Medicas; Portugal
Fil: Wegener, Christian. Universidade Nova de Lisboa. Faculdade de Ciencias Medicas; Portugal
Fil: Gontijo, Alisson. Universidade Nova de Lisboa. Faculdade de Ciencias Medicas; Portugal
Fil: Garelli, Andres. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
V Taller de Biología Celular y del Desarrollo
Chascomús
Argentina
Comité Organizador del V Taller de Biología Celular y del Desarrollo
Materia
DROSOPHILA
BEHAVIOR
NEUROPEPTIDE
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/206026
Acceder
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network_name_str CONICET Digital (CONICET)
spelling Myoinhibiting peptide signaling via sex peptide receptor is critical for proper glue expulsion and spreading behavior during drosophila developmentFernandez Acosta, MagdalenaHeredia, FabianaMenezes, JulianeZanini, RebecaPrüger, KatjaDronseck, AgustinaArana, MaitePereirinha, JoanaLeal, AnaVeenstra, Jan A.Wegener, ChristianGontijo, AlissonGarelli, AndresDROSOPHILABEHAVIORNEUROPEPTIDEhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Drosophila pupariation consists of a series of innate behaviors and morphogenetic changes that reduce predation and desiccation during metamorphosis via the formation of a compacted and hardened pupal case (puparium) from the larval cuticle and its firm attachment to a substrate. The latter is achieved by a rapid and highly-stereotyped behavior, glue expulsion and spreading behavior (GSB), which is preceded by strong body-remodelling contractions, termed pre-GSB. These behaviors must be executed in order and precede cuticle hardening to ensure proper pupariation. The steroid hormone ecdysone coordinates the whole pupariation process, inducing initiation of pre-GSB by an unknown mechanism and its progression to stronger contractions and ultimately to GSB by inducing a cuticle epidermis-to-CNS neuron relaxin-like Dilp8-Lgr3 signaling event. The factors, if any, that induce GSB and post-GSB behaviors downstream of ecdysone remain to be defined. Here we use neuronal-specific RNA interference against a series of neuropeptides to identify Myoinhibiting peptide (MIP) as a critical peptide required for proper GSB. Behavioral monitoring using a muscle calcium reporter (GCamp) shows that the execution of GSB is abnormal in the absence of MIP. Epistasis assays show that MIP acts spatially and temporally downstream of the Dilp8-Lgr3 pathway. Cell type-specific MIP RNAi showed that MIP is required in a single bilateral brain neuron for proper GSB. Further experiments show that MIP acts on neurons expressing Sex-peptide receptor (SPR), which is also critical for proper GSB. We propose that MIP is a key modulator of motor circuits during pupariation. Our results significantly advance our molecular and cellular understanding of pupariation control, and contributes to the understanding of how multi-step innate behaviors are coordinated in timeFil: Fernandez Acosta, Magdalena. Universidade Nova de Lisboa. Faculdade de Ciencias Medicas; PortugalFil: Heredia, Fabiana. Universidade Nova de Lisboa. Faculdade de Ciencias Medicas; PortugalFil: Menezes, Juliane. Universidade Nova de Lisboa. Faculdade de Ciencias Medicas; PortugalFil: Zanini, Rebeca. Universidade Nova de Lisboa. Faculdade de Ciencias Medicas; PortugalFil: Prüger, Katja. Universidade Nova de Lisboa. Faculdade de Ciencias Medicas; PortugalFil: Dronseck, Agustina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; ArgentinaFil: Arana, Maite. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; ArgentinaFil: Pereirinha, Joana. Universidade Nova de Lisboa. Faculdade de Ciencias Medicas; PortugalFil: Leal, Ana. Universidade Nova de Lisboa. Faculdade de Ciencias Medicas; PortugalFil: Veenstra, Jan A.. Universidade Nova de Lisboa. Faculdade de Ciencias Medicas; PortugalFil: Wegener, Christian. Universidade Nova de Lisboa. Faculdade de Ciencias Medicas; PortugalFil: Gontijo, Alisson. Universidade Nova de Lisboa. Faculdade de Ciencias Medicas; PortugalFil: Garelli, Andres. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; ArgentinaV Taller de Biología Celular y del DesarrolloChascomúsArgentinaComité Organizador del V Taller de Biología Celular y del DesarrolloComité organizador del Taller de Biología Celular y del Desarrollo2022info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectTallerBookhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/206026Myoinhibiting peptide signaling via sex peptide receptor is critical for proper glue expulsion and spreading behavior during drosophila development; V Taller de Biología Celular y del Desarrollo; Chascomús; Argentina; 2022; 23-24CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://tallerbcd.wixsite.com/tallerbcdNacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2026-02-26T10:31:06Zoai:ri.conicet.gov.ar:11336/206026instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982026-02-26 10:31:06.592CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Myoinhibiting peptide signaling via sex peptide receptor is critical for proper glue expulsion and spreading behavior during drosophila development
title Myoinhibiting peptide signaling via sex peptide receptor is critical for proper glue expulsion and spreading behavior during drosophila development
spellingShingle Myoinhibiting peptide signaling via sex peptide receptor is critical for proper glue expulsion and spreading behavior during drosophila development
Fernandez Acosta, Magdalena
DROSOPHILA
BEHAVIOR
NEUROPEPTIDE
title_short Myoinhibiting peptide signaling via sex peptide receptor is critical for proper glue expulsion and spreading behavior during drosophila development
title_full Myoinhibiting peptide signaling via sex peptide receptor is critical for proper glue expulsion and spreading behavior during drosophila development
title_fullStr Myoinhibiting peptide signaling via sex peptide receptor is critical for proper glue expulsion and spreading behavior during drosophila development
title_full_unstemmed Myoinhibiting peptide signaling via sex peptide receptor is critical for proper glue expulsion and spreading behavior during drosophila development
title_sort Myoinhibiting peptide signaling via sex peptide receptor is critical for proper glue expulsion and spreading behavior during drosophila development
dc.creator.none.fl_str_mv Fernandez Acosta, Magdalena
Heredia, Fabiana
Menezes, Juliane
Zanini, Rebeca
Prüger, Katja
Dronseck, Agustina
Arana, Maite
Pereirinha, Joana
Leal, Ana
Veenstra, Jan A.
Wegener, Christian
Gontijo, Alisson
Garelli, Andres
author Fernandez Acosta, Magdalena
author_facet Fernandez Acosta, Magdalena
Heredia, Fabiana
Menezes, Juliane
Zanini, Rebeca
Prüger, Katja
Dronseck, Agustina
Arana, Maite
Pereirinha, Joana
Leal, Ana
Veenstra, Jan A.
Wegener, Christian
Gontijo, Alisson
Garelli, Andres
author_role author
author2 Heredia, Fabiana
Menezes, Juliane
Zanini, Rebeca
Prüger, Katja
Dronseck, Agustina
Arana, Maite
Pereirinha, Joana
Leal, Ana
Veenstra, Jan A.
Wegener, Christian
Gontijo, Alisson
Garelli, Andres
author2_role author
author
author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv DROSOPHILA
BEHAVIOR
NEUROPEPTIDE
topic DROSOPHILA
BEHAVIOR
NEUROPEPTIDE
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Drosophila pupariation consists of a series of innate behaviors and morphogenetic changes that reduce predation and desiccation during metamorphosis via the formation of a compacted and hardened pupal case (puparium) from the larval cuticle and its firm attachment to a substrate. The latter is achieved by a rapid and highly-stereotyped behavior, glue expulsion and spreading behavior (GSB), which is preceded by strong body-remodelling contractions, termed pre-GSB. These behaviors must be executed in order and precede cuticle hardening to ensure proper pupariation. The steroid hormone ecdysone coordinates the whole pupariation process, inducing initiation of pre-GSB by an unknown mechanism and its progression to stronger contractions and ultimately to GSB by inducing a cuticle epidermis-to-CNS neuron relaxin-like Dilp8-Lgr3 signaling event. The factors, if any, that induce GSB and post-GSB behaviors downstream of ecdysone remain to be defined. Here we use neuronal-specific RNA interference against a series of neuropeptides to identify Myoinhibiting peptide (MIP) as a critical peptide required for proper GSB. Behavioral monitoring using a muscle calcium reporter (GCamp) shows that the execution of GSB is abnormal in the absence of MIP. Epistasis assays show that MIP acts spatially and temporally downstream of the Dilp8-Lgr3 pathway. Cell type-specific MIP RNAi showed that MIP is required in a single bilateral brain neuron for proper GSB. Further experiments show that MIP acts on neurons expressing Sex-peptide receptor (SPR), which is also critical for proper GSB. We propose that MIP is a key modulator of motor circuits during pupariation. Our results significantly advance our molecular and cellular understanding of pupariation control, and contributes to the understanding of how multi-step innate behaviors are coordinated in time
Fil: Fernandez Acosta, Magdalena. Universidade Nova de Lisboa. Faculdade de Ciencias Medicas; Portugal
Fil: Heredia, Fabiana. Universidade Nova de Lisboa. Faculdade de Ciencias Medicas; Portugal
Fil: Menezes, Juliane. Universidade Nova de Lisboa. Faculdade de Ciencias Medicas; Portugal
Fil: Zanini, Rebeca. Universidade Nova de Lisboa. Faculdade de Ciencias Medicas; Portugal
Fil: Prüger, Katja. Universidade Nova de Lisboa. Faculdade de Ciencias Medicas; Portugal
Fil: Dronseck, Agustina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Fil: Arana, Maite. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Fil: Pereirinha, Joana. Universidade Nova de Lisboa. Faculdade de Ciencias Medicas; Portugal
Fil: Leal, Ana. Universidade Nova de Lisboa. Faculdade de Ciencias Medicas; Portugal
Fil: Veenstra, Jan A.. Universidade Nova de Lisboa. Faculdade de Ciencias Medicas; Portugal
Fil: Wegener, Christian. Universidade Nova de Lisboa. Faculdade de Ciencias Medicas; Portugal
Fil: Gontijo, Alisson. Universidade Nova de Lisboa. Faculdade de Ciencias Medicas; Portugal
Fil: Garelli, Andres. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
V Taller de Biología Celular y del Desarrollo
Chascomús
Argentina
Comité Organizador del V Taller de Biología Celular y del Desarrollo
description Drosophila pupariation consists of a series of innate behaviors and morphogenetic changes that reduce predation and desiccation during metamorphosis via the formation of a compacted and hardened pupal case (puparium) from the larval cuticle and its firm attachment to a substrate. The latter is achieved by a rapid and highly-stereotyped behavior, glue expulsion and spreading behavior (GSB), which is preceded by strong body-remodelling contractions, termed pre-GSB. These behaviors must be executed in order and precede cuticle hardening to ensure proper pupariation. The steroid hormone ecdysone coordinates the whole pupariation process, inducing initiation of pre-GSB by an unknown mechanism and its progression to stronger contractions and ultimately to GSB by inducing a cuticle epidermis-to-CNS neuron relaxin-like Dilp8-Lgr3 signaling event. The factors, if any, that induce GSB and post-GSB behaviors downstream of ecdysone remain to be defined. Here we use neuronal-specific RNA interference against a series of neuropeptides to identify Myoinhibiting peptide (MIP) as a critical peptide required for proper GSB. Behavioral monitoring using a muscle calcium reporter (GCamp) shows that the execution of GSB is abnormal in the absence of MIP. Epistasis assays show that MIP acts spatially and temporally downstream of the Dilp8-Lgr3 pathway. Cell type-specific MIP RNAi showed that MIP is required in a single bilateral brain neuron for proper GSB. Further experiments show that MIP acts on neurons expressing Sex-peptide receptor (SPR), which is also critical for proper GSB. We propose that MIP is a key modulator of motor circuits during pupariation. Our results significantly advance our molecular and cellular understanding of pupariation control, and contributes to the understanding of how multi-step innate behaviors are coordinated in time
publishDate 2022
dc.date.none.fl_str_mv 2022
dc.type.none.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/conferenceObject
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Book
http://purl.org/coar/resource_type/c_5794
info:ar-repo/semantics/documentoDeConferencia
status_str publishedVersion
format conferenceObject
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/206026
Myoinhibiting peptide signaling via sex peptide receptor is critical for proper glue expulsion and spreading behavior during drosophila development; V Taller de Biología Celular y del Desarrollo; Chascomús; Argentina; 2022; 23-24
CONICET Digital
CONICET
url http://hdl.handle.net/11336/206026
identifier_str_mv Myoinhibiting peptide signaling via sex peptide receptor is critical for proper glue expulsion and spreading behavior during drosophila development; V Taller de Biología Celular y del Desarrollo; Chascomús; Argentina; 2022; 23-24
CONICET Digital
CONICET
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language eng
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application/pdf
application/pdf
dc.coverage.none.fl_str_mv Nacional
dc.publisher.none.fl_str_mv Comité organizador del Taller de Biología Celular y del Desarrollo
publisher.none.fl_str_mv Comité organizador del Taller de Biología Celular y del Desarrollo
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
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