Alkaloid profiling and anticholinesterase activity of south american Lycopodiaceae species

Autores
Konrath, Eduardo Luis; Ortega, María Gabriela; Bordignon, Sergio de Loreto; Apel, Miriam Anders; Henriques, Amalia Teresinha; Cabrera, Jose Luis
Año de publicación
2011
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The alkaloid extracts of four Huperzia and one Lycopodiella species, from Brazilian habitats, were tested for their in vitro anticholinesterase activities. IC(50) values showed a potent acetylcholinesterase inhibition for H. reflexa (0.11 ± 0.05 μg/mL), followed by H. quadrifariata (2.0 ± 0.3 μg/mL), H. acerosa (5.5 ± 0.9 μg/mL), H. heterocarpon (25.6 ± 2.7 μg/mL) and L. cernua (42.6 ± 1.5 μg/mL). A lower inhibition of butyrylcholinesterase was observed for all species with the exception of H. heterocarpon (8.3 ± 0.9 μg/mL), whose alkaloid extract presented a selectivity for pseudocholinesterase. Moreover, the chemical study of the bioactive extracts performed by GC-MS, revealed the presence of a number of Lycopodium alkaloids belonging to the lycopodane, flabellidane and cernuane groups. Surprisingly, the potent acetylcholinesterase inhibitors huperzines A and B were not detected in the extracts, suggesting that other alkaloids may be responsible for such an effect.
Fil: Konrath, Eduardo Luis. Universidade Federal do Rio Grande do Sul. Faculdade de Farmácia. Programa de Pós Graduação em Ciências Farmacêuticas; Brasil
Fil: Ortega, María Gabriela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto Multidisciplinario de Biología Vegetal. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Instituto Multidisciplinario de Biología Vegetal; Argentina. Universidad Nacional de Cordoba. Facultad de Ciencias Quimicas. Departamento de Farmacia. Catedra de Farmacognosia; Argentina
Fil: Bordignon, Sergio de Loreto. Centro Universitário La Salle; Brasil
Fil: Apel, Miriam Anders. Universidade Federal do Rio Grande do Sul. Faculdade de Farmácia. Programa de Pós Graduação em Ciências Farmacêuticas; Brasil
Fil: Henriques, Amalia Teresinha. Universidade Federal do Rio Grande do Sul. Faculdade de Farmácia. Programa de Pós Graduação em Ciências Farmacêuticas; Brasil
Fil: Cabrera, Jose Luis. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto Multidisciplinario de Biología Vegetal. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Instituto Multidisciplinario de Biología Vegetal; Argentina. Universidad Nacional de Cordoba. Facultad de Ciencias Quimicas. Departamento de Farmacia. Catedra de Farmacognosia; Argentina
Materia
Huperzia Acerosa
Huperzia Heterocarpon
Huperzia Quadrifariata
Huperzia Reflexa
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/18079
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/18079
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Alkaloid profiling and anticholinesterase activity of south american Lycopodiaceae speciesKonrath, Eduardo LuisOrtega, María GabrielaBordignon, Sergio de LoretoApel, Miriam AndersHenriques, Amalia TeresinhaCabrera, Jose LuisHuperzia AcerosaHuperzia HeterocarponHuperzia QuadrifariataHuperzia Reflexahttps://purl.org/becyt/ford/1.4https://purl.org/becyt/ford/1The alkaloid extracts of four Huperzia and one Lycopodiella species, from Brazilian habitats, were tested for their in vitro anticholinesterase activities. IC(50) values showed a potent acetylcholinesterase inhibition for H. reflexa (0.11 ± 0.05 μg/mL), followed by H. quadrifariata (2.0 ± 0.3 μg/mL), H. acerosa (5.5 ± 0.9 μg/mL), H. heterocarpon (25.6 ± 2.7 μg/mL) and L. cernua (42.6 ± 1.5 μg/mL). A lower inhibition of butyrylcholinesterase was observed for all species with the exception of H. heterocarpon (8.3 ± 0.9 μg/mL), whose alkaloid extract presented a selectivity for pseudocholinesterase. Moreover, the chemical study of the bioactive extracts performed by GC-MS, revealed the presence of a number of Lycopodium alkaloids belonging to the lycopodane, flabellidane and cernuane groups. Surprisingly, the potent acetylcholinesterase inhibitors huperzines A and B were not detected in the extracts, suggesting that other alkaloids may be responsible for such an effect.Fil: Konrath, Eduardo Luis. Universidade Federal do Rio Grande do Sul. Faculdade de Farmácia. Programa de Pós Graduação em Ciências Farmacêuticas; BrasilFil: Ortega, María Gabriela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto Multidisciplinario de Biología Vegetal. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Instituto Multidisciplinario de Biología Vegetal; Argentina. Universidad Nacional de Cordoba. Facultad de Ciencias Quimicas. Departamento de Farmacia. Catedra de Farmacognosia; ArgentinaFil: Bordignon, Sergio de Loreto. Centro Universitário La Salle; BrasilFil: Apel, Miriam Anders. Universidade Federal do Rio Grande do Sul. Faculdade de Farmácia. Programa de Pós Graduação em Ciências Farmacêuticas; BrasilFil: Henriques, Amalia Teresinha. Universidade Federal do Rio Grande do Sul. Faculdade de Farmácia. Programa de Pós Graduação em Ciências Farmacêuticas; BrasilFil: Cabrera, Jose Luis. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto Multidisciplinario de Biología Vegetal. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Instituto Multidisciplinario de Biología Vegetal; Argentina. Universidad Nacional de Cordoba. Facultad de Ciencias Quimicas. Departamento de Farmacia. Catedra de Farmacognosia; ArgentinaTaylor & Francis Ltd2011-10info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/18079Konrath, Eduardo Luis; Ortega, María Gabriela; Bordignon, Sergio de Loreto; Apel, Miriam Anders; Henriques, Amalia Teresinha; et al.; Alkaloid profiling and anticholinesterase activity of south american Lycopodiaceae species; Taylor & Francis Ltd; Journal Of Enzyme Inhibition And Medicinal Chemistry; 28; 1; 10-2011; 218-2221475-63661475-6374CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://www.tandfonline.com/doi/full/10.3109/14756366.2011.633908info:eu-repo/semantics/altIdentifier/doi/10.3109/14756366.2011.633908info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T15:19:11Zoai:ri.conicet.gov.ar:11336/18079instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 15:19:11.505CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Alkaloid profiling and anticholinesterase activity of south american Lycopodiaceae species
title Alkaloid profiling and anticholinesterase activity of south american Lycopodiaceae species
spellingShingle Alkaloid profiling and anticholinesterase activity of south american Lycopodiaceae species
Konrath, Eduardo Luis
Huperzia Acerosa
Huperzia Heterocarpon
Huperzia Quadrifariata
Huperzia Reflexa
title_short Alkaloid profiling and anticholinesterase activity of south american Lycopodiaceae species
title_full Alkaloid profiling and anticholinesterase activity of south american Lycopodiaceae species
title_fullStr Alkaloid profiling and anticholinesterase activity of south american Lycopodiaceae species
title_full_unstemmed Alkaloid profiling and anticholinesterase activity of south american Lycopodiaceae species
title_sort Alkaloid profiling and anticholinesterase activity of south american Lycopodiaceae species
dc.creator.none.fl_str_mv Konrath, Eduardo Luis
Ortega, María Gabriela
Bordignon, Sergio de Loreto
Apel, Miriam Anders
Henriques, Amalia Teresinha
Cabrera, Jose Luis
author Konrath, Eduardo Luis
author_facet Konrath, Eduardo Luis
Ortega, María Gabriela
Bordignon, Sergio de Loreto
Apel, Miriam Anders
Henriques, Amalia Teresinha
Cabrera, Jose Luis
author_role author
author2 Ortega, María Gabriela
Bordignon, Sergio de Loreto
Apel, Miriam Anders
Henriques, Amalia Teresinha
Cabrera, Jose Luis
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Huperzia Acerosa
Huperzia Heterocarpon
Huperzia Quadrifariata
Huperzia Reflexa
topic Huperzia Acerosa
Huperzia Heterocarpon
Huperzia Quadrifariata
Huperzia Reflexa
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.4
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv The alkaloid extracts of four Huperzia and one Lycopodiella species, from Brazilian habitats, were tested for their in vitro anticholinesterase activities. IC(50) values showed a potent acetylcholinesterase inhibition for H. reflexa (0.11 ± 0.05 μg/mL), followed by H. quadrifariata (2.0 ± 0.3 μg/mL), H. acerosa (5.5 ± 0.9 μg/mL), H. heterocarpon (25.6 ± 2.7 μg/mL) and L. cernua (42.6 ± 1.5 μg/mL). A lower inhibition of butyrylcholinesterase was observed for all species with the exception of H. heterocarpon (8.3 ± 0.9 μg/mL), whose alkaloid extract presented a selectivity for pseudocholinesterase. Moreover, the chemical study of the bioactive extracts performed by GC-MS, revealed the presence of a number of Lycopodium alkaloids belonging to the lycopodane, flabellidane and cernuane groups. Surprisingly, the potent acetylcholinesterase inhibitors huperzines A and B were not detected in the extracts, suggesting that other alkaloids may be responsible for such an effect.
Fil: Konrath, Eduardo Luis. Universidade Federal do Rio Grande do Sul. Faculdade de Farmácia. Programa de Pós Graduação em Ciências Farmacêuticas; Brasil
Fil: Ortega, María Gabriela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto Multidisciplinario de Biología Vegetal. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Instituto Multidisciplinario de Biología Vegetal; Argentina. Universidad Nacional de Cordoba. Facultad de Ciencias Quimicas. Departamento de Farmacia. Catedra de Farmacognosia; Argentina
Fil: Bordignon, Sergio de Loreto. Centro Universitário La Salle; Brasil
Fil: Apel, Miriam Anders. Universidade Federal do Rio Grande do Sul. Faculdade de Farmácia. Programa de Pós Graduação em Ciências Farmacêuticas; Brasil
Fil: Henriques, Amalia Teresinha. Universidade Federal do Rio Grande do Sul. Faculdade de Farmácia. Programa de Pós Graduação em Ciências Farmacêuticas; Brasil
Fil: Cabrera, Jose Luis. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto Multidisciplinario de Biología Vegetal. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Instituto Multidisciplinario de Biología Vegetal; Argentina. Universidad Nacional de Cordoba. Facultad de Ciencias Quimicas. Departamento de Farmacia. Catedra de Farmacognosia; Argentina
description The alkaloid extracts of four Huperzia and one Lycopodiella species, from Brazilian habitats, were tested for their in vitro anticholinesterase activities. IC(50) values showed a potent acetylcholinesterase inhibition for H. reflexa (0.11 ± 0.05 μg/mL), followed by H. quadrifariata (2.0 ± 0.3 μg/mL), H. acerosa (5.5 ± 0.9 μg/mL), H. heterocarpon (25.6 ± 2.7 μg/mL) and L. cernua (42.6 ± 1.5 μg/mL). A lower inhibition of butyrylcholinesterase was observed for all species with the exception of H. heterocarpon (8.3 ± 0.9 μg/mL), whose alkaloid extract presented a selectivity for pseudocholinesterase. Moreover, the chemical study of the bioactive extracts performed by GC-MS, revealed the presence of a number of Lycopodium alkaloids belonging to the lycopodane, flabellidane and cernuane groups. Surprisingly, the potent acetylcholinesterase inhibitors huperzines A and B were not detected in the extracts, suggesting that other alkaloids may be responsible for such an effect.
publishDate 2011
dc.date.none.fl_str_mv 2011-10
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/18079
Konrath, Eduardo Luis; Ortega, María Gabriela; Bordignon, Sergio de Loreto; Apel, Miriam Anders; Henriques, Amalia Teresinha; et al.; Alkaloid profiling and anticholinesterase activity of south american Lycopodiaceae species; Taylor & Francis Ltd; Journal Of Enzyme Inhibition And Medicinal Chemistry; 28; 1; 10-2011; 218-222
1475-6366
1475-6374
CONICET Digital
CONICET
url http://hdl.handle.net/11336/18079
identifier_str_mv Konrath, Eduardo Luis; Ortega, María Gabriela; Bordignon, Sergio de Loreto; Apel, Miriam Anders; Henriques, Amalia Teresinha; et al.; Alkaloid profiling and anticholinesterase activity of south american Lycopodiaceae species; Taylor & Francis Ltd; Journal Of Enzyme Inhibition And Medicinal Chemistry; 28; 1; 10-2011; 218-222
1475-6366
1475-6374
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://www.tandfonline.com/doi/full/10.3109/14756366.2011.633908
info:eu-repo/semantics/altIdentifier/doi/10.3109/14756366.2011.633908
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Taylor & Francis Ltd
publisher.none.fl_str_mv Taylor & Francis Ltd
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.22299

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