Key design factors affecting microbial community composition and pathogenic organisms removal in horizontal subsurface flow constructed wetlands

Autores
Morató, Jordi; Codony, Francesc; Sánchez, Olga; Perez, Leonardo Martin; García, Joan; Mas, Jordi
Año de publicación
2014
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Constructed wetlands constitute an interesting option forwastewater reuse since high concentrations of contaminants and pathogenic microorganisms can be removed with these natural treatment systems. In this work, the role of key design factors which could affect microbial removal and wetland performance, such as granular media, water depth and season effect was evaluated in a pilot system consisting of eight parallel horizontal subsurface flow (HSSF) constructed wetlands treating urban wastewater from Les Franqueses del Vallès (Barcelona, Spain). Gravel biofilm as well as influent and effluent water samples of these systems were taken in order to detect the presence of bacterial indicators such as total coliforms (TC), Escherichia coli, fecal enterococci (FE), Clostridium perfringens, and other microbial groups such as Pseudomonas and Aeromonas. The overall microbial inactivation ratio ranged between 1.4 and 2.9 log-units for heterotrophic plate counts (HPC), from 1.2 to 2.2 log units for total coliforms (TC) and from 1.4 to 2.3 log units for E. coli. The presence of fine granulometry strongly influenced the removal of all the bacterial groups analyzed. This effect was significant for TC (p = 0.009), E. coli (p = 0.004), and FE (p = 0.012). Shallow HSSF constructed wetlands were more effective for removing Clostridium spores (p = 0.039), and were also more efficient for removing TC (p = 0.011) and E. coli (p = 0.013) when fine granulometry was used. On the other hand, changes in the total bacterial community from gravel biofilm were examined by using denaturing gradient gel electrophoresis (DGGE) and sequencing of polymerase chain reaction (PCR)-amplified fragments of the 16S rRNA gene recovered from DGGE bands. Cluster analysis of the DGGE banding pattern from the different wetlands showed that microbial assemblages separated according to water depth, and sequences of different phylogenetic groups, such as Alpha, Beta and Delta-Proteobacteria, Nitrospirae, Bacteroidetes, Acidobacteria, Firmicutes, Synergistetes and Deferribacteres could be retrieved from DGGE bands.
Fil: Morató, Jordi. Universidad Politecnica de Catalunya; España
Fil: Codony, Francesc. Universidad Politecnica de Catalunya; España
Fil: Sánchez, Olga. Universidad Autonoma de Barcelona. Departamento de Genetica y Microbiologia; España
Fil: Perez, Leonardo Martin. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Instituto de Química Rosario; Argentina. Universidad Politecnica de Catalunya; España
Fil: García, Joan. Universidad Politecnica de Catalunya; España
Fil: Mas, Jordi. Universidad Autonoma de Barcelona. Departamento de Genetica y Microbiologia; España
Materia
Constructed Wetlands
Horizontal Subsurface Flow
Design Factors
Municipal Wastewater Treatment
Gravel Biofilm Examination
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/5988
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/5988
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Key design factors affecting microbial community composition and pathogenic organisms removal in horizontal subsurface flow constructed wetlandsMorató, JordiCodony, FrancescSánchez, OlgaPerez, Leonardo MartinGarcía, JoanMas, JordiConstructed WetlandsHorizontal Subsurface FlowDesign FactorsMunicipal Wastewater TreatmentGravel Biofilm Examinationhttps://purl.org/becyt/ford/2.8https://purl.org/becyt/ford/2Constructed wetlands constitute an interesting option forwastewater reuse since high concentrations of contaminants and pathogenic microorganisms can be removed with these natural treatment systems. In this work, the role of key design factors which could affect microbial removal and wetland performance, such as granular media, water depth and season effect was evaluated in a pilot system consisting of eight parallel horizontal subsurface flow (HSSF) constructed wetlands treating urban wastewater from Les Franqueses del Vallès (Barcelona, Spain). Gravel biofilm as well as influent and effluent water samples of these systems were taken in order to detect the presence of bacterial indicators such as total coliforms (TC), Escherichia coli, fecal enterococci (FE), Clostridium perfringens, and other microbial groups such as Pseudomonas and Aeromonas. The overall microbial inactivation ratio ranged between 1.4 and 2.9 log-units for heterotrophic plate counts (HPC), from 1.2 to 2.2 log units for total coliforms (TC) and from 1.4 to 2.3 log units for E. coli. The presence of fine granulometry strongly influenced the removal of all the bacterial groups analyzed. This effect was significant for TC (p = 0.009), E. coli (p = 0.004), and FE (p = 0.012). Shallow HSSF constructed wetlands were more effective for removing Clostridium spores (p = 0.039), and were also more efficient for removing TC (p = 0.011) and E. coli (p = 0.013) when fine granulometry was used. On the other hand, changes in the total bacterial community from gravel biofilm were examined by using denaturing gradient gel electrophoresis (DGGE) and sequencing of polymerase chain reaction (PCR)-amplified fragments of the 16S rRNA gene recovered from DGGE bands. Cluster analysis of the DGGE banding pattern from the different wetlands showed that microbial assemblages separated according to water depth, and sequences of different phylogenetic groups, such as Alpha, Beta and Delta-Proteobacteria, Nitrospirae, Bacteroidetes, Acidobacteria, Firmicutes, Synergistetes and Deferribacteres could be retrieved from DGGE bands.Fil: Morató, Jordi. Universidad Politecnica de Catalunya; EspañaFil: Codony, Francesc. Universidad Politecnica de Catalunya; EspañaFil: Sánchez, Olga. Universidad Autonoma de Barcelona. Departamento de Genetica y Microbiologia; EspañaFil: Perez, Leonardo Martin. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Instituto de Química Rosario; Argentina. Universidad Politecnica de Catalunya; EspañaFil: García, Joan. Universidad Politecnica de Catalunya; EspañaFil: Mas, Jordi. Universidad Autonoma de Barcelona. Departamento de Genetica y Microbiologia; EspañaElsevier2014-05info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/5988Morató, Jordi; Codony, Francesc; Sánchez, Olga; Perez, Leonardo Martin; García, Joan; et al.; Key design factors affecting microbial community composition and pathogenic organisms removal in horizontal subsurface flow constructed wetlands; Elsevier; Science of the Total Environment; 481; 5-2014; 81-890048-9697enginfo:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0048969714000825info:eu-repo/semantics/altIdentifier/doi/info:eu-repo/semantics/altIdentifier/doi/10.1016/j.scitotenv.2014.01.068info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:36:35Zoai:ri.conicet.gov.ar:11336/5988instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:36:35.459CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Key design factors affecting microbial community composition and pathogenic organisms removal in horizontal subsurface flow constructed wetlands
title Key design factors affecting microbial community composition and pathogenic organisms removal in horizontal subsurface flow constructed wetlands
spellingShingle Key design factors affecting microbial community composition and pathogenic organisms removal in horizontal subsurface flow constructed wetlands
Morató, Jordi
Constructed Wetlands
Horizontal Subsurface Flow
Design Factors
Municipal Wastewater Treatment
Gravel Biofilm Examination
title_short Key design factors affecting microbial community composition and pathogenic organisms removal in horizontal subsurface flow constructed wetlands
title_full Key design factors affecting microbial community composition and pathogenic organisms removal in horizontal subsurface flow constructed wetlands
title_fullStr Key design factors affecting microbial community composition and pathogenic organisms removal in horizontal subsurface flow constructed wetlands
title_full_unstemmed Key design factors affecting microbial community composition and pathogenic organisms removal in horizontal subsurface flow constructed wetlands
title_sort Key design factors affecting microbial community composition and pathogenic organisms removal in horizontal subsurface flow constructed wetlands
dc.creator.none.fl_str_mv Morató, Jordi
Codony, Francesc
Sánchez, Olga
Perez, Leonardo Martin
García, Joan
Mas, Jordi
author Morató, Jordi
author_facet Morató, Jordi
Codony, Francesc
Sánchez, Olga
Perez, Leonardo Martin
García, Joan
Mas, Jordi
author_role author
author2 Codony, Francesc
Sánchez, Olga
Perez, Leonardo Martin
García, Joan
Mas, Jordi
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Constructed Wetlands
Horizontal Subsurface Flow
Design Factors
Municipal Wastewater Treatment
Gravel Biofilm Examination
topic Constructed Wetlands
Horizontal Subsurface Flow
Design Factors
Municipal Wastewater Treatment
Gravel Biofilm Examination
purl_subject.fl_str_mv https://purl.org/becyt/ford/2.8
https://purl.org/becyt/ford/2
dc.description.none.fl_txt_mv Constructed wetlands constitute an interesting option forwastewater reuse since high concentrations of contaminants and pathogenic microorganisms can be removed with these natural treatment systems. In this work, the role of key design factors which could affect microbial removal and wetland performance, such as granular media, water depth and season effect was evaluated in a pilot system consisting of eight parallel horizontal subsurface flow (HSSF) constructed wetlands treating urban wastewater from Les Franqueses del Vallès (Barcelona, Spain). Gravel biofilm as well as influent and effluent water samples of these systems were taken in order to detect the presence of bacterial indicators such as total coliforms (TC), Escherichia coli, fecal enterococci (FE), Clostridium perfringens, and other microbial groups such as Pseudomonas and Aeromonas. The overall microbial inactivation ratio ranged between 1.4 and 2.9 log-units for heterotrophic plate counts (HPC), from 1.2 to 2.2 log units for total coliforms (TC) and from 1.4 to 2.3 log units for E. coli. The presence of fine granulometry strongly influenced the removal of all the bacterial groups analyzed. This effect was significant for TC (p = 0.009), E. coli (p = 0.004), and FE (p = 0.012). Shallow HSSF constructed wetlands were more effective for removing Clostridium spores (p = 0.039), and were also more efficient for removing TC (p = 0.011) and E. coli (p = 0.013) when fine granulometry was used. On the other hand, changes in the total bacterial community from gravel biofilm were examined by using denaturing gradient gel electrophoresis (DGGE) and sequencing of polymerase chain reaction (PCR)-amplified fragments of the 16S rRNA gene recovered from DGGE bands. Cluster analysis of the DGGE banding pattern from the different wetlands showed that microbial assemblages separated according to water depth, and sequences of different phylogenetic groups, such as Alpha, Beta and Delta-Proteobacteria, Nitrospirae, Bacteroidetes, Acidobacteria, Firmicutes, Synergistetes and Deferribacteres could be retrieved from DGGE bands.
Fil: Morató, Jordi. Universidad Politecnica de Catalunya; España
Fil: Codony, Francesc. Universidad Politecnica de Catalunya; España
Fil: Sánchez, Olga. Universidad Autonoma de Barcelona. Departamento de Genetica y Microbiologia; España
Fil: Perez, Leonardo Martin. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Instituto de Química Rosario; Argentina. Universidad Politecnica de Catalunya; España
Fil: García, Joan. Universidad Politecnica de Catalunya; España
Fil: Mas, Jordi. Universidad Autonoma de Barcelona. Departamento de Genetica y Microbiologia; España
description Constructed wetlands constitute an interesting option forwastewater reuse since high concentrations of contaminants and pathogenic microorganisms can be removed with these natural treatment systems. In this work, the role of key design factors which could affect microbial removal and wetland performance, such as granular media, water depth and season effect was evaluated in a pilot system consisting of eight parallel horizontal subsurface flow (HSSF) constructed wetlands treating urban wastewater from Les Franqueses del Vallès (Barcelona, Spain). Gravel biofilm as well as influent and effluent water samples of these systems were taken in order to detect the presence of bacterial indicators such as total coliforms (TC), Escherichia coli, fecal enterococci (FE), Clostridium perfringens, and other microbial groups such as Pseudomonas and Aeromonas. The overall microbial inactivation ratio ranged between 1.4 and 2.9 log-units for heterotrophic plate counts (HPC), from 1.2 to 2.2 log units for total coliforms (TC) and from 1.4 to 2.3 log units for E. coli. The presence of fine granulometry strongly influenced the removal of all the bacterial groups analyzed. This effect was significant for TC (p = 0.009), E. coli (p = 0.004), and FE (p = 0.012). Shallow HSSF constructed wetlands were more effective for removing Clostridium spores (p = 0.039), and were also more efficient for removing TC (p = 0.011) and E. coli (p = 0.013) when fine granulometry was used. On the other hand, changes in the total bacterial community from gravel biofilm were examined by using denaturing gradient gel electrophoresis (DGGE) and sequencing of polymerase chain reaction (PCR)-amplified fragments of the 16S rRNA gene recovered from DGGE bands. Cluster analysis of the DGGE banding pattern from the different wetlands showed that microbial assemblages separated according to water depth, and sequences of different phylogenetic groups, such as Alpha, Beta and Delta-Proteobacteria, Nitrospirae, Bacteroidetes, Acidobacteria, Firmicutes, Synergistetes and Deferribacteres could be retrieved from DGGE bands.
publishDate 2014
dc.date.none.fl_str_mv 2014-05
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/5988
Morató, Jordi; Codony, Francesc; Sánchez, Olga; Perez, Leonardo Martin; García, Joan; et al.; Key design factors affecting microbial community composition and pathogenic organisms removal in horizontal subsurface flow constructed wetlands; Elsevier; Science of the Total Environment; 481; 5-2014; 81-89
0048-9697
url http://hdl.handle.net/11336/5988
identifier_str_mv Morató, Jordi; Codony, Francesc; Sánchez, Olga; Perez, Leonardo Martin; García, Joan; et al.; Key design factors affecting microbial community composition and pathogenic organisms removal in horizontal subsurface flow constructed wetlands; Elsevier; Science of the Total Environment; 481; 5-2014; 81-89
0048-9697
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0048969714000825
info:eu-repo/semantics/altIdentifier/doi/
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.scitotenv.2014.01.068
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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