In vitro inhibition of Paenibacillus larvae by different extracts and pure compounds from Flourensia spp

Autores
Reyes, Maria Guadalupe; Torres, María Julia; Maggi, Matías Daniel; Marioli, Juan Miguel; Gil, Roberto Ricardo; Sosa, Virginia Estela; Uriburu Monasterio, Maria Laura; Audisio, Marcela Carina
Año de publicación
2013
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Paenibacillus larvae, a sporulating Gram-positive bacterium, is the etiological agent of American foulbrood disease in Apis mellifera L. Plant extracts could be a natural alternative to control this pathology. The current study assessed the anti-. P. larvae effect of extracts and pure principal products from the Flourensia genus: F. riparia, F. fiebrigii and F. tortuosa. Their inhibitory effect was assayed against different P. larvae strains according to the disk diffusion technique and subsequently, the minimal inhibitory concentrations (MIC) of extracts by the agar dilution method was determined. Furthermore, toxicity of the most effective extracts against P. larvae was tested in bees. All extracts inhibited growth of the different P. larvae strains assayed. However, the magnitude of the antagonistic effect depended on the chemical nature of the extract and the P. larvae strain. Chloroform extracts (CE) and ethyl ether extracts (ETE) from F. riparia and ETE from F. fiebrigii were most active against P. larvae Azul, the most sensitive indicator strain with MIC values of 250. ppm (CE) and 2000. ppm (ETE) for F. riparia, and 2500. ppm (ETE) for F. fiebrigii. Hexane extracts from the three species did not present any significant inhibitory effect. These results would indicate that one or some of the more polar compounds would cause inhibition of this pathogen. Toxicity assays demonstrated that even the highest concentrations assayed (125,000. ppm) did not show lethal effects on exposed bees during in vitro conditions. © 2013 Elsevier B.V.
Fil: Reyes, Maria Guadalupe. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Salta. Instituto de Investigación Para la Industria Química (i); Argentina
Fil: Torres, María Julia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Salta. Instituto de Investigación Para la Industria Química (i); Argentina
Fil: Maggi, Matías Daniel. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Departamento de Biología. Laboratorio de Artrópodos; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Marioli, Juan Miguel. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Química; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Gil, Roberto Ricardo. University Of Carnegie Mellon; Estados Unidos. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Sosa, Virginia Estela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Instituto Multidisciplinario de Biología Vegetal (p); Argentina
Fil: Uriburu Monasterio, Maria Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Salta. Instituto de Investigación para la Industria Química (i); Argentina
Fil: Audisio, Marcela Carina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Salta. Instituto de Investigación Para la Industria Química (i); Argentina
Materia
ANTIMICROBIAL ACTIVITY
FLUORENSIA FIEBRIGII
FLUORENSIA RIPARIA
HONEYBEE
PAENIBACILLUS LARVAE
PLANT EXTRACTS
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/4732
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/4732
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling In vitro inhibition of Paenibacillus larvae by different extracts and pure compounds from Flourensia sppReyes, Maria GuadalupeTorres, María JuliaMaggi, Matías DanielMarioli, Juan MiguelGil, Roberto RicardoSosa, Virginia EstelaUriburu Monasterio, Maria LauraAudisio, Marcela CarinaANTIMICROBIAL ACTIVITYFLUORENSIA FIEBRIGIIFLUORENSIA RIPARIAHONEYBEEPAENIBACILLUS LARVAEPLANT EXTRACTShttps://purl.org/becyt/ford/4.1https://purl.org/becyt/ford/4Paenibacillus larvae, a sporulating Gram-positive bacterium, is the etiological agent of American foulbrood disease in Apis mellifera L. Plant extracts could be a natural alternative to control this pathology. The current study assessed the anti-. P. larvae effect of extracts and pure principal products from the Flourensia genus: F. riparia, F. fiebrigii and F. tortuosa. Their inhibitory effect was assayed against different P. larvae strains according to the disk diffusion technique and subsequently, the minimal inhibitory concentrations (MIC) of extracts by the agar dilution method was determined. Furthermore, toxicity of the most effective extracts against P. larvae was tested in bees. All extracts inhibited growth of the different P. larvae strains assayed. However, the magnitude of the antagonistic effect depended on the chemical nature of the extract and the P. larvae strain. Chloroform extracts (CE) and ethyl ether extracts (ETE) from F. riparia and ETE from F. fiebrigii were most active against P. larvae Azul, the most sensitive indicator strain with MIC values of 250. ppm (CE) and 2000. ppm (ETE) for F. riparia, and 2500. ppm (ETE) for F. fiebrigii. Hexane extracts from the three species did not present any significant inhibitory effect. These results would indicate that one or some of the more polar compounds would cause inhibition of this pathogen. Toxicity assays demonstrated that even the highest concentrations assayed (125,000. ppm) did not show lethal effects on exposed bees during in vitro conditions. © 2013 Elsevier B.V.Fil: Reyes, Maria Guadalupe. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Salta. Instituto de Investigación Para la Industria Química (i); ArgentinaFil: Torres, María Julia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Salta. Instituto de Investigación Para la Industria Química (i); ArgentinaFil: Maggi, Matías Daniel. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Departamento de Biología. Laboratorio de Artrópodos; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Marioli, Juan Miguel. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Química; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Gil, Roberto Ricardo. University Of Carnegie Mellon; Estados Unidos. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Sosa, Virginia Estela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Instituto Multidisciplinario de Biología Vegetal (p); ArgentinaFil: Uriburu Monasterio, Maria Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Salta. Instituto de Investigación para la Industria Química (i); ArgentinaFil: Audisio, Marcela Carina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Salta. Instituto de Investigación Para la Industria Química (i); ArgentinaElsevier Science2013-10info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/4732Reyes, Maria Guadalupe; Torres, María Julia; Maggi, Matías Daniel; Marioli, Juan Miguel; Gil, Roberto Ricardo; et al.; In vitro inhibition of Paenibacillus larvae by different extracts and pure compounds from Flourensia spp; Elsevier Science; Industrial Crops and Products; 50; 10-2013; 758-7630926-6690enginfo:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0926669013003993info:eu-repo/semantics/altIdentifier/doi/10.1016/j.indcrop.2013.07.062info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-29T11:18:23Zoai:ri.conicet.gov.ar:11336/4732instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-29 11:18:24.007CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv In vitro inhibition of Paenibacillus larvae by different extracts and pure compounds from Flourensia spp
title In vitro inhibition of Paenibacillus larvae by different extracts and pure compounds from Flourensia spp
spellingShingle In vitro inhibition of Paenibacillus larvae by different extracts and pure compounds from Flourensia spp
Reyes, Maria Guadalupe
ANTIMICROBIAL ACTIVITY
FLUORENSIA FIEBRIGII
FLUORENSIA RIPARIA
HONEYBEE
PAENIBACILLUS LARVAE
PLANT EXTRACTS
title_short In vitro inhibition of Paenibacillus larvae by different extracts and pure compounds from Flourensia spp
title_full In vitro inhibition of Paenibacillus larvae by different extracts and pure compounds from Flourensia spp
title_fullStr In vitro inhibition of Paenibacillus larvae by different extracts and pure compounds from Flourensia spp
title_full_unstemmed In vitro inhibition of Paenibacillus larvae by different extracts and pure compounds from Flourensia spp
title_sort In vitro inhibition of Paenibacillus larvae by different extracts and pure compounds from Flourensia spp
dc.creator.none.fl_str_mv Reyes, Maria Guadalupe
Torres, María Julia
Maggi, Matías Daniel
Marioli, Juan Miguel
Gil, Roberto Ricardo
Sosa, Virginia Estela
Uriburu Monasterio, Maria Laura
Audisio, Marcela Carina
author Reyes, Maria Guadalupe
author_facet Reyes, Maria Guadalupe
Torres, María Julia
Maggi, Matías Daniel
Marioli, Juan Miguel
Gil, Roberto Ricardo
Sosa, Virginia Estela
Uriburu Monasterio, Maria Laura
Audisio, Marcela Carina
author_role author
author2 Torres, María Julia
Maggi, Matías Daniel
Marioli, Juan Miguel
Gil, Roberto Ricardo
Sosa, Virginia Estela
Uriburu Monasterio, Maria Laura
Audisio, Marcela Carina
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv ANTIMICROBIAL ACTIVITY
FLUORENSIA FIEBRIGII
FLUORENSIA RIPARIA
HONEYBEE
PAENIBACILLUS LARVAE
PLANT EXTRACTS
topic ANTIMICROBIAL ACTIVITY
FLUORENSIA FIEBRIGII
FLUORENSIA RIPARIA
HONEYBEE
PAENIBACILLUS LARVAE
PLANT EXTRACTS
purl_subject.fl_str_mv https://purl.org/becyt/ford/4.1
https://purl.org/becyt/ford/4
dc.description.none.fl_txt_mv Paenibacillus larvae, a sporulating Gram-positive bacterium, is the etiological agent of American foulbrood disease in Apis mellifera L. Plant extracts could be a natural alternative to control this pathology. The current study assessed the anti-. P. larvae effect of extracts and pure principal products from the Flourensia genus: F. riparia, F. fiebrigii and F. tortuosa. Their inhibitory effect was assayed against different P. larvae strains according to the disk diffusion technique and subsequently, the minimal inhibitory concentrations (MIC) of extracts by the agar dilution method was determined. Furthermore, toxicity of the most effective extracts against P. larvae was tested in bees. All extracts inhibited growth of the different P. larvae strains assayed. However, the magnitude of the antagonistic effect depended on the chemical nature of the extract and the P. larvae strain. Chloroform extracts (CE) and ethyl ether extracts (ETE) from F. riparia and ETE from F. fiebrigii were most active against P. larvae Azul, the most sensitive indicator strain with MIC values of 250. ppm (CE) and 2000. ppm (ETE) for F. riparia, and 2500. ppm (ETE) for F. fiebrigii. Hexane extracts from the three species did not present any significant inhibitory effect. These results would indicate that one or some of the more polar compounds would cause inhibition of this pathogen. Toxicity assays demonstrated that even the highest concentrations assayed (125,000. ppm) did not show lethal effects on exposed bees during in vitro conditions. © 2013 Elsevier B.V.
Fil: Reyes, Maria Guadalupe. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Salta. Instituto de Investigación Para la Industria Química (i); Argentina
Fil: Torres, María Julia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Salta. Instituto de Investigación Para la Industria Química (i); Argentina
Fil: Maggi, Matías Daniel. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Departamento de Biología. Laboratorio de Artrópodos; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Marioli, Juan Miguel. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Química; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Gil, Roberto Ricardo. University Of Carnegie Mellon; Estados Unidos. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Sosa, Virginia Estela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Instituto Multidisciplinario de Biología Vegetal (p); Argentina
Fil: Uriburu Monasterio, Maria Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Salta. Instituto de Investigación para la Industria Química (i); Argentina
Fil: Audisio, Marcela Carina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Salta. Instituto de Investigación Para la Industria Química (i); Argentina
description Paenibacillus larvae, a sporulating Gram-positive bacterium, is the etiological agent of American foulbrood disease in Apis mellifera L. Plant extracts could be a natural alternative to control this pathology. The current study assessed the anti-. P. larvae effect of extracts and pure principal products from the Flourensia genus: F. riparia, F. fiebrigii and F. tortuosa. Their inhibitory effect was assayed against different P. larvae strains according to the disk diffusion technique and subsequently, the minimal inhibitory concentrations (MIC) of extracts by the agar dilution method was determined. Furthermore, toxicity of the most effective extracts against P. larvae was tested in bees. All extracts inhibited growth of the different P. larvae strains assayed. However, the magnitude of the antagonistic effect depended on the chemical nature of the extract and the P. larvae strain. Chloroform extracts (CE) and ethyl ether extracts (ETE) from F. riparia and ETE from F. fiebrigii were most active against P. larvae Azul, the most sensitive indicator strain with MIC values of 250. ppm (CE) and 2000. ppm (ETE) for F. riparia, and 2500. ppm (ETE) for F. fiebrigii. Hexane extracts from the three species did not present any significant inhibitory effect. These results would indicate that one or some of the more polar compounds would cause inhibition of this pathogen. Toxicity assays demonstrated that even the highest concentrations assayed (125,000. ppm) did not show lethal effects on exposed bees during in vitro conditions. © 2013 Elsevier B.V.
publishDate 2013
dc.date.none.fl_str_mv 2013-10
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/4732
Reyes, Maria Guadalupe; Torres, María Julia; Maggi, Matías Daniel; Marioli, Juan Miguel; Gil, Roberto Ricardo; et al.; In vitro inhibition of Paenibacillus larvae by different extracts and pure compounds from Flourensia spp; Elsevier Science; Industrial Crops and Products; 50; 10-2013; 758-763
0926-6690
url http://hdl.handle.net/11336/4732
identifier_str_mv Reyes, Maria Guadalupe; Torres, María Julia; Maggi, Matías Daniel; Marioli, Juan Miguel; Gil, Roberto Ricardo; et al.; In vitro inhibition of Paenibacillus larvae by different extracts and pure compounds from Flourensia spp; Elsevier Science; Industrial Crops and Products; 50; 10-2013; 758-763
0926-6690
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0926669013003993
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.indcrop.2013.07.062
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Science
publisher.none.fl_str_mv Elsevier Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.10058

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