Transforming Renal Diagnosis: Graphene‐Enhanced Lab‐On‐a‐Chip for Multiplexed Kidney Biomarker Detection in Capillary Blood

Autores
Diforti, Joaquin Felipe; Cunningham, Thomas; Zegalo, Zaira; Piccinini, Esteban; Marmisollé, Waldemar Alejandro; Piccinini, Jose M.; Azzaroni, Omar
Año de publicación
2024
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Chronic kidney disease (CKD) is a significant global health concern, impacting over 10% of the world population. Despite advances in home-based treatments, CKD diagnosis and monitoring remain centralized in large laboratories. This work reports on the development of a Graphene-based Lab-On-a-Chip (G-LOC) for the self-testing of multiple renal function biomarkers in capillary blood. G-LOC integrates bioelectronic sensors with a 3D-printed microfluidic system that enables the multiplex quantification of urea, potassium, sodium, and chloride, from one drop of blood. The potentials of three graphene sensors modified with ion-selective membranes and enzymes are simultaneously measured. The analytical performance of the test is evaluated in terms of linearity, accuracy, and coefficient of variability (CV). Accuracy values higher than 98.7%, and CV values lower than 10.8% are obtained for all the biomarkers. Correlation and Bland–Altman plots show good correlation (slopes in the range of 0.94–1.15) and high agreement of G-LOC with a reference method. It is also demonstrated that the test can correctly differentiate biomarker levels normally obtained for healthy people, early-stage CKD, and end-stage CKD. Finally, user experience is studied with a group of untrained volunteers who highlight the simple usability of the test and its suitability for at-home diagnostics.
Fil: Diforti, Joaquin Felipe. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas; Argentina
Fil: Cunningham, Thomas. No especifíca;
Fil: Zegalo, Zaira. No especifíca;
Fil: Piccinini, Esteban. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas; Argentina
Fil: Marmisollé, Waldemar Alejandro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas; Argentina
Fil: Piccinini, Jose M.. No especifíca;
Fil: Azzaroni, Omar. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas; Argentina
Materia
gFETs
diagnosis
renal
multiplex
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/262569
Acceder
id CONICETDig_6a48fb6387ff432f681a6d752d46156d
oai_identifier_str oai:ri.conicet.gov.ar:11336/262569
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Transforming Renal Diagnosis: Graphene‐Enhanced Lab‐On‐a‐Chip for Multiplexed Kidney Biomarker Detection in Capillary BloodDiforti, Joaquin FelipeCunningham, ThomasZegalo, ZairaPiccinini, EstebanMarmisollé, Waldemar AlejandroPiccinini, Jose M.Azzaroni, OmargFETsdiagnosisrenalmultiplexhttps://purl.org/becyt/ford/1.4https://purl.org/becyt/ford/1Chronic kidney disease (CKD) is a significant global health concern, impacting over 10% of the world population. Despite advances in home-based treatments, CKD diagnosis and monitoring remain centralized in large laboratories. This work reports on the development of a Graphene-based Lab-On-a-Chip (G-LOC) for the self-testing of multiple renal function biomarkers in capillary blood. G-LOC integrates bioelectronic sensors with a 3D-printed microfluidic system that enables the multiplex quantification of urea, potassium, sodium, and chloride, from one drop of blood. The potentials of three graphene sensors modified with ion-selective membranes and enzymes are simultaneously measured. The analytical performance of the test is evaluated in terms of linearity, accuracy, and coefficient of variability (CV). Accuracy values higher than 98.7%, and CV values lower than 10.8% are obtained for all the biomarkers. Correlation and Bland–Altman plots show good correlation (slopes in the range of 0.94–1.15) and high agreement of G-LOC with a reference method. It is also demonstrated that the test can correctly differentiate biomarker levels normally obtained for healthy people, early-stage CKD, and end-stage CKD. Finally, user experience is studied with a group of untrained volunteers who highlight the simple usability of the test and its suitability for at-home diagnostics.Fil: Diforti, Joaquin Felipe. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas; ArgentinaFil: Cunningham, Thomas. No especifíca;Fil: Zegalo, Zaira. No especifíca;Fil: Piccinini, Esteban. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas; ArgentinaFil: Marmisollé, Waldemar Alejandro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas; ArgentinaFil: Piccinini, Jose M.. No especifíca;Fil: Azzaroni, Omar. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas; ArgentinaWiley2024-07info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/262569Diforti, Joaquin Felipe; Cunningham, Thomas; Zegalo, Zaira; Piccinini, Esteban; Marmisollé, Waldemar Alejandro; et al.; Transforming Renal Diagnosis: Graphene‐Enhanced Lab‐On‐a‐Chip for Multiplexed Kidney Biomarker Detection in Capillary Blood; Wiley; Advanced Sensor Research; 3; 11; 7-2024; 1-102751-1219CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/10.1002/adsr.202400061info:eu-repo/semantics/altIdentifier/doi/10.1002/adsr.202400061info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-11-12T09:57:06Zoai:ri.conicet.gov.ar:11336/262569instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-11-12 09:57:06.669CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Transforming Renal Diagnosis: Graphene‐Enhanced Lab‐On‐a‐Chip for Multiplexed Kidney Biomarker Detection in Capillary Blood
title Transforming Renal Diagnosis: Graphene‐Enhanced Lab‐On‐a‐Chip for Multiplexed Kidney Biomarker Detection in Capillary Blood
spellingShingle Transforming Renal Diagnosis: Graphene‐Enhanced Lab‐On‐a‐Chip for Multiplexed Kidney Biomarker Detection in Capillary Blood
Diforti, Joaquin Felipe
gFETs
diagnosis
renal
multiplex
title_short Transforming Renal Diagnosis: Graphene‐Enhanced Lab‐On‐a‐Chip for Multiplexed Kidney Biomarker Detection in Capillary Blood
title_full Transforming Renal Diagnosis: Graphene‐Enhanced Lab‐On‐a‐Chip for Multiplexed Kidney Biomarker Detection in Capillary Blood
title_fullStr Transforming Renal Diagnosis: Graphene‐Enhanced Lab‐On‐a‐Chip for Multiplexed Kidney Biomarker Detection in Capillary Blood
title_full_unstemmed Transforming Renal Diagnosis: Graphene‐Enhanced Lab‐On‐a‐Chip for Multiplexed Kidney Biomarker Detection in Capillary Blood
title_sort Transforming Renal Diagnosis: Graphene‐Enhanced Lab‐On‐a‐Chip for Multiplexed Kidney Biomarker Detection in Capillary Blood
dc.creator.none.fl_str_mv Diforti, Joaquin Felipe
Cunningham, Thomas
Zegalo, Zaira
Piccinini, Esteban
Marmisollé, Waldemar Alejandro
Piccinini, Jose M.
Azzaroni, Omar
author Diforti, Joaquin Felipe
author_facet Diforti, Joaquin Felipe
Cunningham, Thomas
Zegalo, Zaira
Piccinini, Esteban
Marmisollé, Waldemar Alejandro
Piccinini, Jose M.
Azzaroni, Omar
author_role author
author2 Cunningham, Thomas
Zegalo, Zaira
Piccinini, Esteban
Marmisollé, Waldemar Alejandro
Piccinini, Jose M.
Azzaroni, Omar
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv gFETs
diagnosis
renal
multiplex
topic gFETs
diagnosis
renal
multiplex
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.4
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Chronic kidney disease (CKD) is a significant global health concern, impacting over 10% of the world population. Despite advances in home-based treatments, CKD diagnosis and monitoring remain centralized in large laboratories. This work reports on the development of a Graphene-based Lab-On-a-Chip (G-LOC) for the self-testing of multiple renal function biomarkers in capillary blood. G-LOC integrates bioelectronic sensors with a 3D-printed microfluidic system that enables the multiplex quantification of urea, potassium, sodium, and chloride, from one drop of blood. The potentials of three graphene sensors modified with ion-selective membranes and enzymes are simultaneously measured. The analytical performance of the test is evaluated in terms of linearity, accuracy, and coefficient of variability (CV). Accuracy values higher than 98.7%, and CV values lower than 10.8% are obtained for all the biomarkers. Correlation and Bland–Altman plots show good correlation (slopes in the range of 0.94–1.15) and high agreement of G-LOC with a reference method. It is also demonstrated that the test can correctly differentiate biomarker levels normally obtained for healthy people, early-stage CKD, and end-stage CKD. Finally, user experience is studied with a group of untrained volunteers who highlight the simple usability of the test and its suitability for at-home diagnostics.
Fil: Diforti, Joaquin Felipe. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas; Argentina
Fil: Cunningham, Thomas. No especifíca;
Fil: Zegalo, Zaira. No especifíca;
Fil: Piccinini, Esteban. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas; Argentina
Fil: Marmisollé, Waldemar Alejandro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas; Argentina
Fil: Piccinini, Jose M.. No especifíca;
Fil: Azzaroni, Omar. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas; Argentina
description Chronic kidney disease (CKD) is a significant global health concern, impacting over 10% of the world population. Despite advances in home-based treatments, CKD diagnosis and monitoring remain centralized in large laboratories. This work reports on the development of a Graphene-based Lab-On-a-Chip (G-LOC) for the self-testing of multiple renal function biomarkers in capillary blood. G-LOC integrates bioelectronic sensors with a 3D-printed microfluidic system that enables the multiplex quantification of urea, potassium, sodium, and chloride, from one drop of blood. The potentials of three graphene sensors modified with ion-selective membranes and enzymes are simultaneously measured. The analytical performance of the test is evaluated in terms of linearity, accuracy, and coefficient of variability (CV). Accuracy values higher than 98.7%, and CV values lower than 10.8% are obtained for all the biomarkers. Correlation and Bland–Altman plots show good correlation (slopes in the range of 0.94–1.15) and high agreement of G-LOC with a reference method. It is also demonstrated that the test can correctly differentiate biomarker levels normally obtained for healthy people, early-stage CKD, and end-stage CKD. Finally, user experience is studied with a group of untrained volunteers who highlight the simple usability of the test and its suitability for at-home diagnostics.
publishDate 2024
dc.date.none.fl_str_mv 2024-07
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/262569
Diforti, Joaquin Felipe; Cunningham, Thomas; Zegalo, Zaira; Piccinini, Esteban; Marmisollé, Waldemar Alejandro; et al.; Transforming Renal Diagnosis: Graphene‐Enhanced Lab‐On‐a‐Chip for Multiplexed Kidney Biomarker Detection in Capillary Blood; Wiley; Advanced Sensor Research; 3; 11; 7-2024; 1-10
2751-1219
CONICET Digital
CONICET
url http://hdl.handle.net/11336/262569
identifier_str_mv Diforti, Joaquin Felipe; Cunningham, Thomas; Zegalo, Zaira; Piccinini, Esteban; Marmisollé, Waldemar Alejandro; et al.; Transforming Renal Diagnosis: Graphene‐Enhanced Lab‐On‐a‐Chip for Multiplexed Kidney Biomarker Detection in Capillary Blood; Wiley; Advanced Sensor Research; 3; 11; 7-2024; 1-10
2751-1219
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/10.1002/adsr.202400061
info:eu-repo/semantics/altIdentifier/doi/10.1002/adsr.202400061
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Wiley
publisher.none.fl_str_mv Wiley
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1848598357922021376
score 12.976206

Publicaciones similares