Electroejaculation in the Chinchilla (Chinchilla lanigera): Effects of anesthesia on seminal characteristics

Autores
Busso, Juan Manuel; Ponzio, M. F.; Chiaraviglio, M.; Fiol de Cuneo, M.; Ruiz, R. D.
Año de publicación
2005
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Repetitive electroejaculation is probably extremely stressful in conscious animals and could adversely affect fertility. The present study was designed to (a) evaluate the effects of anesthesia (40 mg ketamine/kg body weight, i.m.) on a method of electroejaculation used previously in conscious chinchillas (Chinchilla lanigera), and (b) determine the quality of the electroejaculated semen obtained under anesthesia. In Experiment 1 (8 animals), a 4 × 4 Latin square design was used to study the effects of anesthesia and ejaculatory voltage on semen collection, ejaculate volume, sperm concentration, motility, viability, response to the hypoosmotic swelling test (HOST), and acrosomal status. In Experiment 2 (12 animals), the effects of differing voltages and the number of stimuli on ejaculation by conscious or anesthetized males was determined. In both experiments, all the conscious animals ejaculated, but only 60% ejaculated under anesthesia and they required more stimuli and higher voltages to achieve ejaculation. Ejaculate volume was significantly lower in anesthetized (<5 μl) than in conscious animals (>40 μl), but sperm concentration was unaffected. None of the indices of sperm quality were affected by anesthesia. The techniques we have developed in anesthetized domestic chinchillas should be applicable to endangered chinchillas in the wild, and though the number of sperm available is reduced, there are still sufficient for assisted reproduction. © 2004 Elsevier Ltd. All rights reserved.
Fil: Busso, Juan Manuel. Universidad Nacional de Córdoba. Facultad de Medicina; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina
Fil: Ponzio, M. F.. Universidad Nacional de Córdoba. Facultad de Medicina; Argentina
Fil: Chiaraviglio, M.. Universidad Nacional de Córdoba; Argentina
Fil: Fiol de Cuneo, M.. Universidad Nacional de Córdoba. Facultad de Medicina; Argentina
Fil: Ruiz, R. D.. Universidad Nacional de Córdoba. Facultad de Medicina; Argentina
Materia
ANESTHESIA
FERTILITY
CHINCHILLA LANIGERA
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/114680
Acceder
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repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Electroejaculation in the Chinchilla (Chinchilla lanigera): Effects of anesthesia on seminal characteristicsBusso, Juan ManuelPonzio, M. F.Chiaraviglio, M.Fiol de Cuneo, M.Ruiz, R. D.ANESTHESIAFERTILITYCHINCHILLA LANIGERAhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Repetitive electroejaculation is probably extremely stressful in conscious animals and could adversely affect fertility. The present study was designed to (a) evaluate the effects of anesthesia (40 mg ketamine/kg body weight, i.m.) on a method of electroejaculation used previously in conscious chinchillas (Chinchilla lanigera), and (b) determine the quality of the electroejaculated semen obtained under anesthesia. In Experiment 1 (8 animals), a 4 × 4 Latin square design was used to study the effects of anesthesia and ejaculatory voltage on semen collection, ejaculate volume, sperm concentration, motility, viability, response to the hypoosmotic swelling test (HOST), and acrosomal status. In Experiment 2 (12 animals), the effects of differing voltages and the number of stimuli on ejaculation by conscious or anesthetized males was determined. In both experiments, all the conscious animals ejaculated, but only 60% ejaculated under anesthesia and they required more stimuli and higher voltages to achieve ejaculation. Ejaculate volume was significantly lower in anesthetized (<5 μl) than in conscious animals (>40 μl), but sperm concentration was unaffected. None of the indices of sperm quality were affected by anesthesia. The techniques we have developed in anesthetized domestic chinchillas should be applicable to endangered chinchillas in the wild, and though the number of sperm available is reduced, there are still sufficient for assisted reproduction. © 2004 Elsevier Ltd. All rights reserved.Fil: Busso, Juan Manuel. Universidad Nacional de Córdoba. Facultad de Medicina; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; ArgentinaFil: Ponzio, M. F.. Universidad Nacional de Córdoba. Facultad de Medicina; ArgentinaFil: Chiaraviglio, M.. Universidad Nacional de Córdoba; ArgentinaFil: Fiol de Cuneo, M.. Universidad Nacional de Córdoba. Facultad de Medicina; ArgentinaFil: Ruiz, R. D.. Universidad Nacional de Córdoba. Facultad de Medicina; ArgentinaElsevier2005-02info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/114680Busso, Juan Manuel; Ponzio, M. F.; Chiaraviglio, M.; Fiol de Cuneo, M.; Ruiz, R. D.; Electroejaculation in the Chinchilla (Chinchilla lanigera): Effects of anesthesia on seminal characteristics; Elsevier; Research in Veterinary Science; 78; 1; 2-2005; 93-970034-5288CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/abs/pii/S0034528804001201?via%3Dihubinfo:eu-repo/semantics/altIdentifier/doi/doi:10.1016/j.rvsc.2004.06.004info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T10:10:56Zoai:ri.conicet.gov.ar:11336/114680instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 10:10:56.444CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Electroejaculation in the Chinchilla (Chinchilla lanigera): Effects of anesthesia on seminal characteristics
title Electroejaculation in the Chinchilla (Chinchilla lanigera): Effects of anesthesia on seminal characteristics
spellingShingle Electroejaculation in the Chinchilla (Chinchilla lanigera): Effects of anesthesia on seminal characteristics
Busso, Juan Manuel
ANESTHESIA
FERTILITY
CHINCHILLA LANIGERA
title_short Electroejaculation in the Chinchilla (Chinchilla lanigera): Effects of anesthesia on seminal characteristics
title_full Electroejaculation in the Chinchilla (Chinchilla lanigera): Effects of anesthesia on seminal characteristics
title_fullStr Electroejaculation in the Chinchilla (Chinchilla lanigera): Effects of anesthesia on seminal characteristics
title_full_unstemmed Electroejaculation in the Chinchilla (Chinchilla lanigera): Effects of anesthesia on seminal characteristics
title_sort Electroejaculation in the Chinchilla (Chinchilla lanigera): Effects of anesthesia on seminal characteristics
dc.creator.none.fl_str_mv Busso, Juan Manuel
Ponzio, M. F.
Chiaraviglio, M.
Fiol de Cuneo, M.
Ruiz, R. D.
author Busso, Juan Manuel
author_facet Busso, Juan Manuel
Ponzio, M. F.
Chiaraviglio, M.
Fiol de Cuneo, M.
Ruiz, R. D.
author_role author
author2 Ponzio, M. F.
Chiaraviglio, M.
Fiol de Cuneo, M.
Ruiz, R. D.
author2_role author
author
author
author
dc.subject.none.fl_str_mv ANESTHESIA
FERTILITY
CHINCHILLA LANIGERA
topic ANESTHESIA
FERTILITY
CHINCHILLA LANIGERA
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Repetitive electroejaculation is probably extremely stressful in conscious animals and could adversely affect fertility. The present study was designed to (a) evaluate the effects of anesthesia (40 mg ketamine/kg body weight, i.m.) on a method of electroejaculation used previously in conscious chinchillas (Chinchilla lanigera), and (b) determine the quality of the electroejaculated semen obtained under anesthesia. In Experiment 1 (8 animals), a 4 × 4 Latin square design was used to study the effects of anesthesia and ejaculatory voltage on semen collection, ejaculate volume, sperm concentration, motility, viability, response to the hypoosmotic swelling test (HOST), and acrosomal status. In Experiment 2 (12 animals), the effects of differing voltages and the number of stimuli on ejaculation by conscious or anesthetized males was determined. In both experiments, all the conscious animals ejaculated, but only 60% ejaculated under anesthesia and they required more stimuli and higher voltages to achieve ejaculation. Ejaculate volume was significantly lower in anesthetized (<5 μl) than in conscious animals (>40 μl), but sperm concentration was unaffected. None of the indices of sperm quality were affected by anesthesia. The techniques we have developed in anesthetized domestic chinchillas should be applicable to endangered chinchillas in the wild, and though the number of sperm available is reduced, there are still sufficient for assisted reproduction. © 2004 Elsevier Ltd. All rights reserved.
Fil: Busso, Juan Manuel. Universidad Nacional de Córdoba. Facultad de Medicina; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina
Fil: Ponzio, M. F.. Universidad Nacional de Córdoba. Facultad de Medicina; Argentina
Fil: Chiaraviglio, M.. Universidad Nacional de Córdoba; Argentina
Fil: Fiol de Cuneo, M.. Universidad Nacional de Córdoba. Facultad de Medicina; Argentina
Fil: Ruiz, R. D.. Universidad Nacional de Córdoba. Facultad de Medicina; Argentina
description Repetitive electroejaculation is probably extremely stressful in conscious animals and could adversely affect fertility. The present study was designed to (a) evaluate the effects of anesthesia (40 mg ketamine/kg body weight, i.m.) on a method of electroejaculation used previously in conscious chinchillas (Chinchilla lanigera), and (b) determine the quality of the electroejaculated semen obtained under anesthesia. In Experiment 1 (8 animals), a 4 × 4 Latin square design was used to study the effects of anesthesia and ejaculatory voltage on semen collection, ejaculate volume, sperm concentration, motility, viability, response to the hypoosmotic swelling test (HOST), and acrosomal status. In Experiment 2 (12 animals), the effects of differing voltages and the number of stimuli on ejaculation by conscious or anesthetized males was determined. In both experiments, all the conscious animals ejaculated, but only 60% ejaculated under anesthesia and they required more stimuli and higher voltages to achieve ejaculation. Ejaculate volume was significantly lower in anesthetized (<5 μl) than in conscious animals (>40 μl), but sperm concentration was unaffected. None of the indices of sperm quality were affected by anesthesia. The techniques we have developed in anesthetized domestic chinchillas should be applicable to endangered chinchillas in the wild, and though the number of sperm available is reduced, there are still sufficient for assisted reproduction. © 2004 Elsevier Ltd. All rights reserved.
publishDate 2005
dc.date.none.fl_str_mv 2005-02
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/114680
Busso, Juan Manuel; Ponzio, M. F.; Chiaraviglio, M.; Fiol de Cuneo, M.; Ruiz, R. D.; Electroejaculation in the Chinchilla (Chinchilla lanigera): Effects of anesthesia on seminal characteristics; Elsevier; Research in Veterinary Science; 78; 1; 2-2005; 93-97
0034-5288
CONICET Digital
CONICET
url http://hdl.handle.net/11336/114680
identifier_str_mv Busso, Juan Manuel; Ponzio, M. F.; Chiaraviglio, M.; Fiol de Cuneo, M.; Ruiz, R. D.; Electroejaculation in the Chinchilla (Chinchilla lanigera): Effects of anesthesia on seminal characteristics; Elsevier; Research in Veterinary Science; 78; 1; 2-2005; 93-97
0034-5288
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/abs/pii/S0034528804001201?via%3Dihub
info:eu-repo/semantics/altIdentifier/doi/doi:10.1016/j.rvsc.2004.06.004
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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