An amperometric biosensor for trans resveratrol determination in aqueous solutions by means of carbon paste electrodes modified with peroxidase basic isoenzymes from brassica napus

Autores
Granero, Adrian Marcelo; Fernández, Héctor; Agostini, Elizabeth; Zon, María Alicia
Año de publicación
2008
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The catalytic properties of peroxidase basic isoenzymes (PBI´s) from Brassica napus towards trans resveratrol (t-Res) oxidation were demonstrated by the first time by conventional uv-visible spectroscopic measurements. The enzymatic reaction rate was studied under different experimental conditions and the kinetics parameters were determined. An amperometric biosensor based on Brassica napus PBI´s to determine t-Res is also proposed by the first time. The method employs a dialysis membrane covered, PBI´s entrapped and ferrocene (Fc)-embedded carbon paste electrode (PBI´s-Fc-CP) and is based on the fact that the decreased amount of H2O2 produced by the action of PBI´s is proportional to the oxidised amount of t-Res in the solution. Comparative amperometric experiments showed that, in spite of PBI´s activity was much lower than commercial horseradish peroxidise (HRP) activity, t-Res was a much better substrate for PBI´s biosensors than those biosensors constructed by using HRP. The PBI´s-Fc-CP biosensors showed a very good stability during at least twenty days. The reproducibility and the repeatability were 4.5% and 8.3%, respectively, showing a good biosensor performance. The calibration curve was linear in the t-Res concentration (ct-Res) range from 1 x 10-6 to 2.5 x 10-5 M, with a sensibility of (2.310.05) x 106 nA M-1. The lowest ct-Res value measured experimentally for a signal to noise ratio of 3:1 was 0.83 M.
Fil: Granero, Adrian Marcelo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Química; Argentina
Fil: Fernández, Héctor. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Química; Argentina
Fil: Agostini, Elizabeth. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Biología Molecular; Argentina
Fil: Zon, María Alicia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Química; Argentina
Materia
TRANS RESVERATROL
BIOSENSOR
BRASSICA NAPUS
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/242481
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/242481
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling An amperometric biosensor for trans resveratrol determination in aqueous solutions by means of carbon paste electrodes modified with peroxidase basic isoenzymes from brassica napusGranero, Adrian MarceloFernández, HéctorAgostini, ElizabethZon, María AliciaTRANS RESVERATROLBIOSENSORBRASSICA NAPUShttps://purl.org/becyt/ford/1.4https://purl.org/becyt/ford/1The catalytic properties of peroxidase basic isoenzymes (PBI´s) from Brassica napus towards trans resveratrol (t-Res) oxidation were demonstrated by the first time by conventional uv-visible spectroscopic measurements. The enzymatic reaction rate was studied under different experimental conditions and the kinetics parameters were determined. An amperometric biosensor based on Brassica napus PBI´s to determine t-Res is also proposed by the first time. The method employs a dialysis membrane covered, PBI´s entrapped and ferrocene (Fc)-embedded carbon paste electrode (PBI´s-Fc-CP) and is based on the fact that the decreased amount of H2O2 produced by the action of PBI´s is proportional to the oxidised amount of t-Res in the solution. Comparative amperometric experiments showed that, in spite of PBI´s activity was much lower than commercial horseradish peroxidise (HRP) activity, t-Res was a much better substrate for PBI´s biosensors than those biosensors constructed by using HRP. The PBI´s-Fc-CP biosensors showed a very good stability during at least twenty days. The reproducibility and the repeatability were 4.5% and 8.3%, respectively, showing a good biosensor performance. The calibration curve was linear in the t-Res concentration (ct-Res) range from 1 x 10-6 to 2.5 x 10-5 M, with a sensibility of (2.310.05) x 106 nA M-1. The lowest ct-Res value measured experimentally for a signal to noise ratio of 3:1 was 0.83 M.Fil: Granero, Adrian Marcelo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Química; ArgentinaFil: Fernández, Héctor. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Química; ArgentinaFil: Agostini, Elizabeth. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Biología Molecular; ArgentinaFil: Zon, María Alicia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Química; ArgentinaWiley VCH Verlag2008-04info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/242481Granero, Adrian Marcelo; Fernández, Héctor; Agostini, Elizabeth; Zon, María Alicia; An amperometric biosensor for trans resveratrol determination in aqueous solutions by means of carbon paste electrodes modified with peroxidase basic isoenzymes from brassica napus; Wiley VCH Verlag; Electroanalysis; 20; 8; 4-2008; 858-8641040-0397CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://analyticalsciencejournals.onlinelibrary.wiley.com/doi/10.1002/elan.200704109info:eu-repo/semantics/altIdentifier/doi/10.1002/elan.200704109info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T15:17:04Zoai:ri.conicet.gov.ar:11336/242481instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 15:17:04.884CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv An amperometric biosensor for trans resveratrol determination in aqueous solutions by means of carbon paste electrodes modified with peroxidase basic isoenzymes from brassica napus
title An amperometric biosensor for trans resveratrol determination in aqueous solutions by means of carbon paste electrodes modified with peroxidase basic isoenzymes from brassica napus
spellingShingle An amperometric biosensor for trans resveratrol determination in aqueous solutions by means of carbon paste electrodes modified with peroxidase basic isoenzymes from brassica napus
Granero, Adrian Marcelo
TRANS RESVERATROL
BIOSENSOR
BRASSICA NAPUS
title_short An amperometric biosensor for trans resveratrol determination in aqueous solutions by means of carbon paste electrodes modified with peroxidase basic isoenzymes from brassica napus
title_full An amperometric biosensor for trans resveratrol determination in aqueous solutions by means of carbon paste electrodes modified with peroxidase basic isoenzymes from brassica napus
title_fullStr An amperometric biosensor for trans resveratrol determination in aqueous solutions by means of carbon paste electrodes modified with peroxidase basic isoenzymes from brassica napus
title_full_unstemmed An amperometric biosensor for trans resveratrol determination in aqueous solutions by means of carbon paste electrodes modified with peroxidase basic isoenzymes from brassica napus
title_sort An amperometric biosensor for trans resveratrol determination in aqueous solutions by means of carbon paste electrodes modified with peroxidase basic isoenzymes from brassica napus
dc.creator.none.fl_str_mv Granero, Adrian Marcelo
Fernández, Héctor
Agostini, Elizabeth
Zon, María Alicia
author Granero, Adrian Marcelo
author_facet Granero, Adrian Marcelo
Fernández, Héctor
Agostini, Elizabeth
Zon, María Alicia
author_role author
author2 Fernández, Héctor
Agostini, Elizabeth
Zon, María Alicia
author2_role author
author
author
dc.subject.none.fl_str_mv TRANS RESVERATROL
BIOSENSOR
BRASSICA NAPUS
topic TRANS RESVERATROL
BIOSENSOR
BRASSICA NAPUS
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.4
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv The catalytic properties of peroxidase basic isoenzymes (PBI´s) from Brassica napus towards trans resveratrol (t-Res) oxidation were demonstrated by the first time by conventional uv-visible spectroscopic measurements. The enzymatic reaction rate was studied under different experimental conditions and the kinetics parameters were determined. An amperometric biosensor based on Brassica napus PBI´s to determine t-Res is also proposed by the first time. The method employs a dialysis membrane covered, PBI´s entrapped and ferrocene (Fc)-embedded carbon paste electrode (PBI´s-Fc-CP) and is based on the fact that the decreased amount of H2O2 produced by the action of PBI´s is proportional to the oxidised amount of t-Res in the solution. Comparative amperometric experiments showed that, in spite of PBI´s activity was much lower than commercial horseradish peroxidise (HRP) activity, t-Res was a much better substrate for PBI´s biosensors than those biosensors constructed by using HRP. The PBI´s-Fc-CP biosensors showed a very good stability during at least twenty days. The reproducibility and the repeatability were 4.5% and 8.3%, respectively, showing a good biosensor performance. The calibration curve was linear in the t-Res concentration (ct-Res) range from 1 x 10-6 to 2.5 x 10-5 M, with a sensibility of (2.310.05) x 106 nA M-1. The lowest ct-Res value measured experimentally for a signal to noise ratio of 3:1 was 0.83 M.
Fil: Granero, Adrian Marcelo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Química; Argentina
Fil: Fernández, Héctor. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Química; Argentina
Fil: Agostini, Elizabeth. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Biología Molecular; Argentina
Fil: Zon, María Alicia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Química; Argentina
description The catalytic properties of peroxidase basic isoenzymes (PBI´s) from Brassica napus towards trans resveratrol (t-Res) oxidation were demonstrated by the first time by conventional uv-visible spectroscopic measurements. The enzymatic reaction rate was studied under different experimental conditions and the kinetics parameters were determined. An amperometric biosensor based on Brassica napus PBI´s to determine t-Res is also proposed by the first time. The method employs a dialysis membrane covered, PBI´s entrapped and ferrocene (Fc)-embedded carbon paste electrode (PBI´s-Fc-CP) and is based on the fact that the decreased amount of H2O2 produced by the action of PBI´s is proportional to the oxidised amount of t-Res in the solution. Comparative amperometric experiments showed that, in spite of PBI´s activity was much lower than commercial horseradish peroxidise (HRP) activity, t-Res was a much better substrate for PBI´s biosensors than those biosensors constructed by using HRP. The PBI´s-Fc-CP biosensors showed a very good stability during at least twenty days. The reproducibility and the repeatability were 4.5% and 8.3%, respectively, showing a good biosensor performance. The calibration curve was linear in the t-Res concentration (ct-Res) range from 1 x 10-6 to 2.5 x 10-5 M, with a sensibility of (2.310.05) x 106 nA M-1. The lowest ct-Res value measured experimentally for a signal to noise ratio of 3:1 was 0.83 M.
publishDate 2008
dc.date.none.fl_str_mv 2008-04
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/242481
Granero, Adrian Marcelo; Fernández, Héctor; Agostini, Elizabeth; Zon, María Alicia; An amperometric biosensor for trans resveratrol determination in aqueous solutions by means of carbon paste electrodes modified with peroxidase basic isoenzymes from brassica napus; Wiley VCH Verlag; Electroanalysis; 20; 8; 4-2008; 858-864
1040-0397
CONICET Digital
CONICET
url http://hdl.handle.net/11336/242481
identifier_str_mv Granero, Adrian Marcelo; Fernández, Héctor; Agostini, Elizabeth; Zon, María Alicia; An amperometric biosensor for trans resveratrol determination in aqueous solutions by means of carbon paste electrodes modified with peroxidase basic isoenzymes from brassica napus; Wiley VCH Verlag; Electroanalysis; 20; 8; 4-2008; 858-864
1040-0397
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://analyticalsciencejournals.onlinelibrary.wiley.com/doi/10.1002/elan.200704109
info:eu-repo/semantics/altIdentifier/doi/10.1002/elan.200704109
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Wiley VCH Verlag
publisher.none.fl_str_mv Wiley VCH Verlag
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.216834

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