Biological Hydrogen Production by Dark Fermentation in a Stirred Tank Reactor and Its Correlation with the pH Time Evolution

Autores
Martinez, Veronica Laura; Salierno, Gabriel Leonardo; García, Rodrigo Enrique; Lavorante, Maria Jose; Galvagno, Miguel Angel; Cassanello Fernandez, Miryam Celeste
Año de publicación
2022
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Dark fermentation is a hydrogen generating process carried out by anaerobic spore-forming bacteria that metabolize carbon sources producing gas and short-chain acids. The process can be controlled, and the hydrogen harvested if bacteria are grown in a reactor with favorable conditions. In this work, bacteria selected from natural sources were grown with a defined culture media, while pH was monitored, with the aim of relating the amount of generated hydrogen to the increase in hydron ion concentration. Therefore, a model based on the acid-base species mass balance is proposed and solved to estimate the lag phase time and measure the hydrogen production efficiency and kinetics. Hydrogen production in a stirred batch reactor was performed for 150–200 h, at given operating conditions using a previously defined growth media, to validate the model. Using the proposed model, the cumulated moles of produced hydrogen correlate well with those predicted from the pH curve. Hence, the modified Gompertz model parameters, largely used for describing the hydrogen generation kinetics by dark fermentation, were estimated from the pH curve and from the experimentally measured generated hydrogen. Satisfactory agreement was found, thus, validating the method.
Fil: Martinez, Veronica Laura. Ministerio de Defensa. Instituto de Investigaciones Científicas y Técnicas para la Defensa; Argentina
Fil: Salierno, Gabriel Leonardo. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Industrias. Instituto de Tecnología de Alimentos y Procesos Quimicos. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Tecnología de Alimentos y Procesos Quimicos.; Argentina
Fil: García, Rodrigo Enrique. Ministerio de Defensa. Armada Argentina. Dirección Gral. de Investigación y Desarrollo de la Ara; Argentina
Fil: Lavorante, Maria Jose. Ministerio de Defensa. Instituto de Investigaciones Científicas y Técnicas para la Defensa; Argentina
Fil: Galvagno, Miguel Angel. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Micología y Botánica. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Micología y Botánica; Argentina
Fil: Cassanello Fernandez, Miryam Celeste. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Industrias. Instituto de Tecnología de Alimentos y Procesos Quimicos. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Tecnología de Alimentos y Procesos Quimicos.; Argentina
Materia
BIOHYDROGEN
DARK FERMENTATION
GOMPERTZ MODEL
PH INFLUENCE
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/213593
Acceder
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network_acronym_str CONICETDig
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network_name_str CONICET Digital (CONICET)
spelling Biological Hydrogen Production by Dark Fermentation in a Stirred Tank Reactor and Its Correlation with the pH Time EvolutionMartinez, Veronica LauraSalierno, Gabriel LeonardoGarcía, Rodrigo EnriqueLavorante, Maria JoseGalvagno, Miguel AngelCassanello Fernandez, Miryam CelesteBIOHYDROGENDARK FERMENTATIONGOMPERTZ MODELPH INFLUENCEhttps://purl.org/becyt/ford/2.9https://purl.org/becyt/ford/2Dark fermentation is a hydrogen generating process carried out by anaerobic spore-forming bacteria that metabolize carbon sources producing gas and short-chain acids. The process can be controlled, and the hydrogen harvested if bacteria are grown in a reactor with favorable conditions. In this work, bacteria selected from natural sources were grown with a defined culture media, while pH was monitored, with the aim of relating the amount of generated hydrogen to the increase in hydron ion concentration. Therefore, a model based on the acid-base species mass balance is proposed and solved to estimate the lag phase time and measure the hydrogen production efficiency and kinetics. Hydrogen production in a stirred batch reactor was performed for 150–200 h, at given operating conditions using a previously defined growth media, to validate the model. Using the proposed model, the cumulated moles of produced hydrogen correlate well with those predicted from the pH curve. Hence, the modified Gompertz model parameters, largely used for describing the hydrogen generation kinetics by dark fermentation, were estimated from the pH curve and from the experimentally measured generated hydrogen. Satisfactory agreement was found, thus, validating the method.Fil: Martinez, Veronica Laura. Ministerio de Defensa. Instituto de Investigaciones Científicas y Técnicas para la Defensa; ArgentinaFil: Salierno, Gabriel Leonardo. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Industrias. Instituto de Tecnología de Alimentos y Procesos Quimicos. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Tecnología de Alimentos y Procesos Quimicos.; ArgentinaFil: García, Rodrigo Enrique. Ministerio de Defensa. Armada Argentina. Dirección Gral. de Investigación y Desarrollo de la Ara; ArgentinaFil: Lavorante, Maria Jose. Ministerio de Defensa. Instituto de Investigaciones Científicas y Técnicas para la Defensa; ArgentinaFil: Galvagno, Miguel Angel. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Micología y Botánica. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Micología y Botánica; ArgentinaFil: Cassanello Fernandez, Miryam Celeste. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Industrias. Instituto de Tecnología de Alimentos y Procesos Quimicos. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Tecnología de Alimentos y Procesos Quimicos.; ArgentinaMDPI2022-11info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/213593Martinez, Veronica Laura; Salierno, Gabriel Leonardo; García, Rodrigo Enrique; Lavorante, Maria Jose; Galvagno, Miguel Angel; et al.; Biological Hydrogen Production by Dark Fermentation in a Stirred Tank Reactor and Its Correlation with the pH Time Evolution; MDPI; Catalysts; 12; 11; 11-2022; 1366-13822073-4344CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.mdpi.com/2073-4344/12/11/1366info:eu-repo/semantics/altIdentifier/doi/10.3390/catal12111366info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:45:02Zoai:ri.conicet.gov.ar:11336/213593instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:45:02.384CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Biological Hydrogen Production by Dark Fermentation in a Stirred Tank Reactor and Its Correlation with the pH Time Evolution
title Biological Hydrogen Production by Dark Fermentation in a Stirred Tank Reactor and Its Correlation with the pH Time Evolution
spellingShingle Biological Hydrogen Production by Dark Fermentation in a Stirred Tank Reactor and Its Correlation with the pH Time Evolution
Martinez, Veronica Laura
BIOHYDROGEN
DARK FERMENTATION
GOMPERTZ MODEL
PH INFLUENCE
title_short Biological Hydrogen Production by Dark Fermentation in a Stirred Tank Reactor and Its Correlation with the pH Time Evolution
title_full Biological Hydrogen Production by Dark Fermentation in a Stirred Tank Reactor and Its Correlation with the pH Time Evolution
title_fullStr Biological Hydrogen Production by Dark Fermentation in a Stirred Tank Reactor and Its Correlation with the pH Time Evolution
title_full_unstemmed Biological Hydrogen Production by Dark Fermentation in a Stirred Tank Reactor and Its Correlation with the pH Time Evolution
title_sort Biological Hydrogen Production by Dark Fermentation in a Stirred Tank Reactor and Its Correlation with the pH Time Evolution
dc.creator.none.fl_str_mv Martinez, Veronica Laura
Salierno, Gabriel Leonardo
García, Rodrigo Enrique
Lavorante, Maria Jose
Galvagno, Miguel Angel
Cassanello Fernandez, Miryam Celeste
author Martinez, Veronica Laura
author_facet Martinez, Veronica Laura
Salierno, Gabriel Leonardo
García, Rodrigo Enrique
Lavorante, Maria Jose
Galvagno, Miguel Angel
Cassanello Fernandez, Miryam Celeste
author_role author
author2 Salierno, Gabriel Leonardo
García, Rodrigo Enrique
Lavorante, Maria Jose
Galvagno, Miguel Angel
Cassanello Fernandez, Miryam Celeste
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv BIOHYDROGEN
DARK FERMENTATION
GOMPERTZ MODEL
PH INFLUENCE
topic BIOHYDROGEN
DARK FERMENTATION
GOMPERTZ MODEL
PH INFLUENCE
purl_subject.fl_str_mv https://purl.org/becyt/ford/2.9
https://purl.org/becyt/ford/2
dc.description.none.fl_txt_mv Dark fermentation is a hydrogen generating process carried out by anaerobic spore-forming bacteria that metabolize carbon sources producing gas and short-chain acids. The process can be controlled, and the hydrogen harvested if bacteria are grown in a reactor with favorable conditions. In this work, bacteria selected from natural sources were grown with a defined culture media, while pH was monitored, with the aim of relating the amount of generated hydrogen to the increase in hydron ion concentration. Therefore, a model based on the acid-base species mass balance is proposed and solved to estimate the lag phase time and measure the hydrogen production efficiency and kinetics. Hydrogen production in a stirred batch reactor was performed for 150–200 h, at given operating conditions using a previously defined growth media, to validate the model. Using the proposed model, the cumulated moles of produced hydrogen correlate well with those predicted from the pH curve. Hence, the modified Gompertz model parameters, largely used for describing the hydrogen generation kinetics by dark fermentation, were estimated from the pH curve and from the experimentally measured generated hydrogen. Satisfactory agreement was found, thus, validating the method.
Fil: Martinez, Veronica Laura. Ministerio de Defensa. Instituto de Investigaciones Científicas y Técnicas para la Defensa; Argentina
Fil: Salierno, Gabriel Leonardo. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Industrias. Instituto de Tecnología de Alimentos y Procesos Quimicos. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Tecnología de Alimentos y Procesos Quimicos.; Argentina
Fil: García, Rodrigo Enrique. Ministerio de Defensa. Armada Argentina. Dirección Gral. de Investigación y Desarrollo de la Ara; Argentina
Fil: Lavorante, Maria Jose. Ministerio de Defensa. Instituto de Investigaciones Científicas y Técnicas para la Defensa; Argentina
Fil: Galvagno, Miguel Angel. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Micología y Botánica. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Micología y Botánica; Argentina
Fil: Cassanello Fernandez, Miryam Celeste. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Industrias. Instituto de Tecnología de Alimentos y Procesos Quimicos. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Tecnología de Alimentos y Procesos Quimicos.; Argentina
description Dark fermentation is a hydrogen generating process carried out by anaerobic spore-forming bacteria that metabolize carbon sources producing gas and short-chain acids. The process can be controlled, and the hydrogen harvested if bacteria are grown in a reactor with favorable conditions. In this work, bacteria selected from natural sources were grown with a defined culture media, while pH was monitored, with the aim of relating the amount of generated hydrogen to the increase in hydron ion concentration. Therefore, a model based on the acid-base species mass balance is proposed and solved to estimate the lag phase time and measure the hydrogen production efficiency and kinetics. Hydrogen production in a stirred batch reactor was performed for 150–200 h, at given operating conditions using a previously defined growth media, to validate the model. Using the proposed model, the cumulated moles of produced hydrogen correlate well with those predicted from the pH curve. Hence, the modified Gompertz model parameters, largely used for describing the hydrogen generation kinetics by dark fermentation, were estimated from the pH curve and from the experimentally measured generated hydrogen. Satisfactory agreement was found, thus, validating the method.
publishDate 2022
dc.date.none.fl_str_mv 2022-11
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/213593
Martinez, Veronica Laura; Salierno, Gabriel Leonardo; García, Rodrigo Enrique; Lavorante, Maria Jose; Galvagno, Miguel Angel; et al.; Biological Hydrogen Production by Dark Fermentation in a Stirred Tank Reactor and Its Correlation with the pH Time Evolution; MDPI; Catalysts; 12; 11; 11-2022; 1366-1382
2073-4344
CONICET Digital
CONICET
url http://hdl.handle.net/11336/213593
identifier_str_mv Martinez, Veronica Laura; Salierno, Gabriel Leonardo; García, Rodrigo Enrique; Lavorante, Maria Jose; Galvagno, Miguel Angel; et al.; Biological Hydrogen Production by Dark Fermentation in a Stirred Tank Reactor and Its Correlation with the pH Time Evolution; MDPI; Catalysts; 12; 11; 11-2022; 1366-1382
2073-4344
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.mdpi.com/2073-4344/12/11/1366
info:eu-repo/semantics/altIdentifier/doi/10.3390/catal12111366
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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