Indocyanine green within glycosylated polymeric micelles as potential image agents to map sentinel lymph nodes and breast cancer
- Autores
- Lecot, Nicole; Fernández Lomónaco, Marcelo; Cerecetto, Hugo; Gambini, Juan Pablo; Cabral, Pablo; Glisoni, Romina Julieta
- Año de publicación
- 2024
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- Indocyanine green (ICG) is an FDA-approved near-infrared (NIR) dye used as a contrast agent for medical diagnosis in such techniques as image-guided surgery (IGS) and IGS-supported mapping for sentinel lymph node biopsy (SNLB). However, there are numerous disadvantages to its use in clinical applications: (i) self-aggregation in solution, (ii) poor targeting and (iii) short half-life in vivo, due to the rapid uptake by the liver. Herein, to overcome these obstacles, we utilized polymeric micelles (PMs) based on the amphiphilic linear and branched block poly(ethylene oxide)–poly(propylene oxide) (PEO–PPO) copolymers (Pluronic® and Tetronic®) for ICG stabilization, vehicleization and to directionally target breast cancer tissues. Because of their singular properties, PMs offer several advantages such as the ability to modify their surfaces with a variety of receptor-targeting ligands and their nano-scale size, which is suitable for taking advantage of the enhanced permeability and retention (EPR) effect for cancer diagnosis. In this work, we prepared ICG within pristine F127 and T1307 and their glucosylated derivatives (F127-Glu and T1307-Glu, respectively). These systems have a sub-30 nm-nanosized hydrodynamic diameter (19–27 nm), moderate negative Z-potentials (until −10 mV), and satisfactory stability in water even after lyophilisation and reconstitution, at 25 and 37 °C, respectively. Particularly, ICG within T1307-Glu PMs displayed maximum solubility and excellent encapsulation efficiency (100%), with a potentially large in vivo uptake according to high specificity and efficacious capture in lymph nodes (LNs) and tumors. All the results presented in this work, indicate that ICG-loaded PMs can potentially be used as image probe agents for IGS, SLNB and breast cancer imaging.
Fil: Lecot, Nicole. Universidad de la República; Uruguay
Fil: Fernández Lomónaco, Marcelo. Universidad de la República; Uruguay
Fil: Cerecetto, Hugo. Universidad de la República; Uruguay
Fil: Gambini, Juan Pablo. Universidad de la República; Uruguay
Fil: Cabral, Pablo. Universidad de la República; Uruguay
Fil: Glisoni, Romina Julieta. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Nanobiotecnología. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Nanobiotecnología; Argentina - Materia
-
INDOCYANINE GREEN
POLYMERIC MICELLES
LYMPH NODES
BREAST CANCER
IMAGE AGENTS - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc/2.5/ar/
- Repositorio
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/261026
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Indocyanine green within glycosylated polymeric micelles as potential image agents to map sentinel lymph nodes and breast cancerLecot, NicoleFernández Lomónaco, MarceloCerecetto, HugoGambini, Juan PabloCabral, PabloGlisoni, Romina JulietaINDOCYANINE GREENPOLYMERIC MICELLESLYMPH NODESBREAST CANCERIMAGE AGENTShttps://purl.org/becyt/ford/2.10https://purl.org/becyt/ford/2Indocyanine green (ICG) is an FDA-approved near-infrared (NIR) dye used as a contrast agent for medical diagnosis in such techniques as image-guided surgery (IGS) and IGS-supported mapping for sentinel lymph node biopsy (SNLB). However, there are numerous disadvantages to its use in clinical applications: (i) self-aggregation in solution, (ii) poor targeting and (iii) short half-life in vivo, due to the rapid uptake by the liver. Herein, to overcome these obstacles, we utilized polymeric micelles (PMs) based on the amphiphilic linear and branched block poly(ethylene oxide)–poly(propylene oxide) (PEO–PPO) copolymers (Pluronic® and Tetronic®) for ICG stabilization, vehicleization and to directionally target breast cancer tissues. Because of their singular properties, PMs offer several advantages such as the ability to modify their surfaces with a variety of receptor-targeting ligands and their nano-scale size, which is suitable for taking advantage of the enhanced permeability and retention (EPR) effect for cancer diagnosis. In this work, we prepared ICG within pristine F127 and T1307 and their glucosylated derivatives (F127-Glu and T1307-Glu, respectively). These systems have a sub-30 nm-nanosized hydrodynamic diameter (19–27 nm), moderate negative Z-potentials (until −10 mV), and satisfactory stability in water even after lyophilisation and reconstitution, at 25 and 37 °C, respectively. Particularly, ICG within T1307-Glu PMs displayed maximum solubility and excellent encapsulation efficiency (100%), with a potentially large in vivo uptake according to high specificity and efficacious capture in lymph nodes (LNs) and tumors. All the results presented in this work, indicate that ICG-loaded PMs can potentially be used as image probe agents for IGS, SLNB and breast cancer imaging.Fil: Lecot, Nicole. Universidad de la República; UruguayFil: Fernández Lomónaco, Marcelo. Universidad de la República; UruguayFil: Cerecetto, Hugo. Universidad de la República; UruguayFil: Gambini, Juan Pablo. Universidad de la República; UruguayFil: Cabral, Pablo. Universidad de la República; UruguayFil: Glisoni, Romina Julieta. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Nanobiotecnología. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Nanobiotecnología; ArgentinaRoyal Society of Chemistry2024-02info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/261026Lecot, Nicole; Fernández Lomónaco, Marcelo; Cerecetto, Hugo; Gambini, Juan Pablo; Cabral, Pablo; et al.; Indocyanine green within glycosylated polymeric micelles as potential image agents to map sentinel lymph nodes and breast cancer; Royal Society of Chemistry; RSC Pharmaceutics; 1; 1; 2-2024; 57-672976-8713CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://xlink.rsc.org/?DOI=D3PM00053Binfo:eu-repo/semantics/altIdentifier/doi/10.1039/D3PM00053Binfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:55:28Zoai:ri.conicet.gov.ar:11336/261026instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:55:28.405CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
dc.title.none.fl_str_mv |
Indocyanine green within glycosylated polymeric micelles as potential image agents to map sentinel lymph nodes and breast cancer |
title |
Indocyanine green within glycosylated polymeric micelles as potential image agents to map sentinel lymph nodes and breast cancer |
spellingShingle |
Indocyanine green within glycosylated polymeric micelles as potential image agents to map sentinel lymph nodes and breast cancer Lecot, Nicole INDOCYANINE GREEN POLYMERIC MICELLES LYMPH NODES BREAST CANCER IMAGE AGENTS |
title_short |
Indocyanine green within glycosylated polymeric micelles as potential image agents to map sentinel lymph nodes and breast cancer |
title_full |
Indocyanine green within glycosylated polymeric micelles as potential image agents to map sentinel lymph nodes and breast cancer |
title_fullStr |
Indocyanine green within glycosylated polymeric micelles as potential image agents to map sentinel lymph nodes and breast cancer |
title_full_unstemmed |
Indocyanine green within glycosylated polymeric micelles as potential image agents to map sentinel lymph nodes and breast cancer |
title_sort |
Indocyanine green within glycosylated polymeric micelles as potential image agents to map sentinel lymph nodes and breast cancer |
dc.creator.none.fl_str_mv |
Lecot, Nicole Fernández Lomónaco, Marcelo Cerecetto, Hugo Gambini, Juan Pablo Cabral, Pablo Glisoni, Romina Julieta |
author |
Lecot, Nicole |
author_facet |
Lecot, Nicole Fernández Lomónaco, Marcelo Cerecetto, Hugo Gambini, Juan Pablo Cabral, Pablo Glisoni, Romina Julieta |
author_role |
author |
author2 |
Fernández Lomónaco, Marcelo Cerecetto, Hugo Gambini, Juan Pablo Cabral, Pablo Glisoni, Romina Julieta |
author2_role |
author author author author author |
dc.subject.none.fl_str_mv |
INDOCYANINE GREEN POLYMERIC MICELLES LYMPH NODES BREAST CANCER IMAGE AGENTS |
topic |
INDOCYANINE GREEN POLYMERIC MICELLES LYMPH NODES BREAST CANCER IMAGE AGENTS |
purl_subject.fl_str_mv |
https://purl.org/becyt/ford/2.10 https://purl.org/becyt/ford/2 |
dc.description.none.fl_txt_mv |
Indocyanine green (ICG) is an FDA-approved near-infrared (NIR) dye used as a contrast agent for medical diagnosis in such techniques as image-guided surgery (IGS) and IGS-supported mapping for sentinel lymph node biopsy (SNLB). However, there are numerous disadvantages to its use in clinical applications: (i) self-aggregation in solution, (ii) poor targeting and (iii) short half-life in vivo, due to the rapid uptake by the liver. Herein, to overcome these obstacles, we utilized polymeric micelles (PMs) based on the amphiphilic linear and branched block poly(ethylene oxide)–poly(propylene oxide) (PEO–PPO) copolymers (Pluronic® and Tetronic®) for ICG stabilization, vehicleization and to directionally target breast cancer tissues. Because of their singular properties, PMs offer several advantages such as the ability to modify their surfaces with a variety of receptor-targeting ligands and their nano-scale size, which is suitable for taking advantage of the enhanced permeability and retention (EPR) effect for cancer diagnosis. In this work, we prepared ICG within pristine F127 and T1307 and their glucosylated derivatives (F127-Glu and T1307-Glu, respectively). These systems have a sub-30 nm-nanosized hydrodynamic diameter (19–27 nm), moderate negative Z-potentials (until −10 mV), and satisfactory stability in water even after lyophilisation and reconstitution, at 25 and 37 °C, respectively. Particularly, ICG within T1307-Glu PMs displayed maximum solubility and excellent encapsulation efficiency (100%), with a potentially large in vivo uptake according to high specificity and efficacious capture in lymph nodes (LNs) and tumors. All the results presented in this work, indicate that ICG-loaded PMs can potentially be used as image probe agents for IGS, SLNB and breast cancer imaging. Fil: Lecot, Nicole. Universidad de la República; Uruguay Fil: Fernández Lomónaco, Marcelo. Universidad de la República; Uruguay Fil: Cerecetto, Hugo. Universidad de la República; Uruguay Fil: Gambini, Juan Pablo. Universidad de la República; Uruguay Fil: Cabral, Pablo. Universidad de la República; Uruguay Fil: Glisoni, Romina Julieta. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Nanobiotecnología. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Nanobiotecnología; Argentina |
description |
Indocyanine green (ICG) is an FDA-approved near-infrared (NIR) dye used as a contrast agent for medical diagnosis in such techniques as image-guided surgery (IGS) and IGS-supported mapping for sentinel lymph node biopsy (SNLB). However, there are numerous disadvantages to its use in clinical applications: (i) self-aggregation in solution, (ii) poor targeting and (iii) short half-life in vivo, due to the rapid uptake by the liver. Herein, to overcome these obstacles, we utilized polymeric micelles (PMs) based on the amphiphilic linear and branched block poly(ethylene oxide)–poly(propylene oxide) (PEO–PPO) copolymers (Pluronic® and Tetronic®) for ICG stabilization, vehicleization and to directionally target breast cancer tissues. Because of their singular properties, PMs offer several advantages such as the ability to modify their surfaces with a variety of receptor-targeting ligands and their nano-scale size, which is suitable for taking advantage of the enhanced permeability and retention (EPR) effect for cancer diagnosis. In this work, we prepared ICG within pristine F127 and T1307 and their glucosylated derivatives (F127-Glu and T1307-Glu, respectively). These systems have a sub-30 nm-nanosized hydrodynamic diameter (19–27 nm), moderate negative Z-potentials (until −10 mV), and satisfactory stability in water even after lyophilisation and reconstitution, at 25 and 37 °C, respectively. Particularly, ICG within T1307-Glu PMs displayed maximum solubility and excellent encapsulation efficiency (100%), with a potentially large in vivo uptake according to high specificity and efficacious capture in lymph nodes (LNs) and tumors. All the results presented in this work, indicate that ICG-loaded PMs can potentially be used as image probe agents for IGS, SLNB and breast cancer imaging. |
publishDate |
2024 |
dc.date.none.fl_str_mv |
2024-02 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/261026 Lecot, Nicole; Fernández Lomónaco, Marcelo; Cerecetto, Hugo; Gambini, Juan Pablo; Cabral, Pablo; et al.; Indocyanine green within glycosylated polymeric micelles as potential image agents to map sentinel lymph nodes and breast cancer; Royal Society of Chemistry; RSC Pharmaceutics; 1; 1; 2-2024; 57-67 2976-8713 CONICET Digital CONICET |
url |
http://hdl.handle.net/11336/261026 |
identifier_str_mv |
Lecot, Nicole; Fernández Lomónaco, Marcelo; Cerecetto, Hugo; Gambini, Juan Pablo; Cabral, Pablo; et al.; Indocyanine green within glycosylated polymeric micelles as potential image agents to map sentinel lymph nodes and breast cancer; Royal Society of Chemistry; RSC Pharmaceutics; 1; 1; 2-2024; 57-67 2976-8713 CONICET Digital CONICET |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/url/http://xlink.rsc.org/?DOI=D3PM00053B info:eu-repo/semantics/altIdentifier/doi/10.1039/D3PM00053B |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc/2.5/ar/ |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by-nc/2.5/ar/ |
dc.format.none.fl_str_mv |
application/pdf application/pdf application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
Royal Society of Chemistry |
publisher.none.fl_str_mv |
Royal Society of Chemistry |
dc.source.none.fl_str_mv |
reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas |
reponame_str |
CONICET Digital (CONICET) |
collection |
CONICET Digital (CONICET) |
instname_str |
Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.name.fl_str_mv |
CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.mail.fl_str_mv |
dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
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1842269345576648704 |
score |
13.13397 |