Indocyanine green within glycosylated polymeric micelles as potential image agents to map sentinel lymph nodes and breast cancer

Autores
Lecot, Nicole; Fernández Lomónaco, Marcelo; Cerecetto, Hugo; Gambini, Juan Pablo; Cabral, Pablo; Glisoni, Romina Julieta
Año de publicación
2024
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Indocyanine green (ICG) is an FDA-approved near-infrared (NIR) dye used as a contrast agent for medical diagnosis in such techniques as image-guided surgery (IGS) and IGS-supported mapping for sentinel lymph node biopsy (SNLB). However, there are numerous disadvantages to its use in clinical applications: (i) self-aggregation in solution, (ii) poor targeting and (iii) short half-life in vivo, due to the rapid uptake by the liver. Herein, to overcome these obstacles, we utilized polymeric micelles (PMs) based on the amphiphilic linear and branched block poly(ethylene oxide)–poly(propylene oxide) (PEO–PPO) copolymers (Pluronic® and Tetronic®) for ICG stabilization, vehicleization and to directionally target breast cancer tissues. Because of their singular properties, PMs offer several advantages such as the ability to modify their surfaces with a variety of receptor-targeting ligands and their nano-scale size, which is suitable for taking advantage of the enhanced permeability and retention (EPR) effect for cancer diagnosis. In this work, we prepared ICG within pristine F127 and T1307 and their glucosylated derivatives (F127-Glu and T1307-Glu, respectively). These systems have a sub-30 nm-nanosized hydrodynamic diameter (19–27 nm), moderate negative Z-potentials (until −10 mV), and satisfactory stability in water even after lyophilisation and reconstitution, at 25 and 37 °C, respectively. Particularly, ICG within T1307-Glu PMs displayed maximum solubility and excellent encapsulation efficiency (100%), with a potentially large in vivo uptake according to high specificity and efficacious capture in lymph nodes (LNs) and tumors. All the results presented in this work, indicate that ICG-loaded PMs can potentially be used as image probe agents for IGS, SLNB and breast cancer imaging.
Fil: Lecot, Nicole. Universidad de la República; Uruguay
Fil: Fernández Lomónaco, Marcelo. Universidad de la República; Uruguay
Fil: Cerecetto, Hugo. Universidad de la República; Uruguay
Fil: Gambini, Juan Pablo. Universidad de la República; Uruguay
Fil: Cabral, Pablo. Universidad de la República; Uruguay
Fil: Glisoni, Romina Julieta. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Nanobiotecnología. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Nanobiotecnología; Argentina
Materia
INDOCYANINE GREEN
POLYMERIC MICELLES
LYMPH NODES
BREAST CANCER
IMAGE AGENTS
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/261026
Acceder
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network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Indocyanine green within glycosylated polymeric micelles as potential image agents to map sentinel lymph nodes and breast cancerLecot, NicoleFernández Lomónaco, MarceloCerecetto, HugoGambini, Juan PabloCabral, PabloGlisoni, Romina JulietaINDOCYANINE GREENPOLYMERIC MICELLESLYMPH NODESBREAST CANCERIMAGE AGENTShttps://purl.org/becyt/ford/2.10https://purl.org/becyt/ford/2Indocyanine green (ICG) is an FDA-approved near-infrared (NIR) dye used as a contrast agent for medical diagnosis in such techniques as image-guided surgery (IGS) and IGS-supported mapping for sentinel lymph node biopsy (SNLB). However, there are numerous disadvantages to its use in clinical applications: (i) self-aggregation in solution, (ii) poor targeting and (iii) short half-life in vivo, due to the rapid uptake by the liver. Herein, to overcome these obstacles, we utilized polymeric micelles (PMs) based on the amphiphilic linear and branched block poly(ethylene oxide)–poly(propylene oxide) (PEO–PPO) copolymers (Pluronic® and Tetronic®) for ICG stabilization, vehicleization and to directionally target breast cancer tissues. Because of their singular properties, PMs offer several advantages such as the ability to modify their surfaces with a variety of receptor-targeting ligands and their nano-scale size, which is suitable for taking advantage of the enhanced permeability and retention (EPR) effect for cancer diagnosis. In this work, we prepared ICG within pristine F127 and T1307 and their glucosylated derivatives (F127-Glu and T1307-Glu, respectively). These systems have a sub-30 nm-nanosized hydrodynamic diameter (19–27 nm), moderate negative Z-potentials (until −10 mV), and satisfactory stability in water even after lyophilisation and reconstitution, at 25 and 37 °C, respectively. Particularly, ICG within T1307-Glu PMs displayed maximum solubility and excellent encapsulation efficiency (100%), with a potentially large in vivo uptake according to high specificity and efficacious capture in lymph nodes (LNs) and tumors. All the results presented in this work, indicate that ICG-loaded PMs can potentially be used as image probe agents for IGS, SLNB and breast cancer imaging.Fil: Lecot, Nicole. Universidad de la República; UruguayFil: Fernández Lomónaco, Marcelo. Universidad de la República; UruguayFil: Cerecetto, Hugo. Universidad de la República; UruguayFil: Gambini, Juan Pablo. Universidad de la República; UruguayFil: Cabral, Pablo. Universidad de la República; UruguayFil: Glisoni, Romina Julieta. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Nanobiotecnología. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Nanobiotecnología; ArgentinaRoyal Society of Chemistry2024-02info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/261026Lecot, Nicole; Fernández Lomónaco, Marcelo; Cerecetto, Hugo; Gambini, Juan Pablo; Cabral, Pablo; et al.; Indocyanine green within glycosylated polymeric micelles as potential image agents to map sentinel lymph nodes and breast cancer; Royal Society of Chemistry; RSC Pharmaceutics; 1; 1; 2-2024; 57-672976-8713CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://xlink.rsc.org/?DOI=D3PM00053Binfo:eu-repo/semantics/altIdentifier/doi/10.1039/D3PM00053Binfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:58:05Zoai:ri.conicet.gov.ar:11336/261026instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:58:05.504CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Indocyanine green within glycosylated polymeric micelles as potential image agents to map sentinel lymph nodes and breast cancer
title Indocyanine green within glycosylated polymeric micelles as potential image agents to map sentinel lymph nodes and breast cancer
spellingShingle Indocyanine green within glycosylated polymeric micelles as potential image agents to map sentinel lymph nodes and breast cancer
Lecot, Nicole
INDOCYANINE GREEN
POLYMERIC MICELLES
LYMPH NODES
BREAST CANCER
IMAGE AGENTS
title_short Indocyanine green within glycosylated polymeric micelles as potential image agents to map sentinel lymph nodes and breast cancer
title_full Indocyanine green within glycosylated polymeric micelles as potential image agents to map sentinel lymph nodes and breast cancer
title_fullStr Indocyanine green within glycosylated polymeric micelles as potential image agents to map sentinel lymph nodes and breast cancer
title_full_unstemmed Indocyanine green within glycosylated polymeric micelles as potential image agents to map sentinel lymph nodes and breast cancer
title_sort Indocyanine green within glycosylated polymeric micelles as potential image agents to map sentinel lymph nodes and breast cancer
dc.creator.none.fl_str_mv Lecot, Nicole
Fernández Lomónaco, Marcelo
Cerecetto, Hugo
Gambini, Juan Pablo
Cabral, Pablo
Glisoni, Romina Julieta
author Lecot, Nicole
author_facet Lecot, Nicole
Fernández Lomónaco, Marcelo
Cerecetto, Hugo
Gambini, Juan Pablo
Cabral, Pablo
Glisoni, Romina Julieta
author_role author
author2 Fernández Lomónaco, Marcelo
Cerecetto, Hugo
Gambini, Juan Pablo
Cabral, Pablo
Glisoni, Romina Julieta
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv INDOCYANINE GREEN
POLYMERIC MICELLES
LYMPH NODES
BREAST CANCER
IMAGE AGENTS
topic INDOCYANINE GREEN
POLYMERIC MICELLES
LYMPH NODES
BREAST CANCER
IMAGE AGENTS
purl_subject.fl_str_mv https://purl.org/becyt/ford/2.10
https://purl.org/becyt/ford/2
dc.description.none.fl_txt_mv Indocyanine green (ICG) is an FDA-approved near-infrared (NIR) dye used as a contrast agent for medical diagnosis in such techniques as image-guided surgery (IGS) and IGS-supported mapping for sentinel lymph node biopsy (SNLB). However, there are numerous disadvantages to its use in clinical applications: (i) self-aggregation in solution, (ii) poor targeting and (iii) short half-life in vivo, due to the rapid uptake by the liver. Herein, to overcome these obstacles, we utilized polymeric micelles (PMs) based on the amphiphilic linear and branched block poly(ethylene oxide)–poly(propylene oxide) (PEO–PPO) copolymers (Pluronic® and Tetronic®) for ICG stabilization, vehicleization and to directionally target breast cancer tissues. Because of their singular properties, PMs offer several advantages such as the ability to modify their surfaces with a variety of receptor-targeting ligands and their nano-scale size, which is suitable for taking advantage of the enhanced permeability and retention (EPR) effect for cancer diagnosis. In this work, we prepared ICG within pristine F127 and T1307 and their glucosylated derivatives (F127-Glu and T1307-Glu, respectively). These systems have a sub-30 nm-nanosized hydrodynamic diameter (19–27 nm), moderate negative Z-potentials (until −10 mV), and satisfactory stability in water even after lyophilisation and reconstitution, at 25 and 37 °C, respectively. Particularly, ICG within T1307-Glu PMs displayed maximum solubility and excellent encapsulation efficiency (100%), with a potentially large in vivo uptake according to high specificity and efficacious capture in lymph nodes (LNs) and tumors. All the results presented in this work, indicate that ICG-loaded PMs can potentially be used as image probe agents for IGS, SLNB and breast cancer imaging.
Fil: Lecot, Nicole. Universidad de la República; Uruguay
Fil: Fernández Lomónaco, Marcelo. Universidad de la República; Uruguay
Fil: Cerecetto, Hugo. Universidad de la República; Uruguay
Fil: Gambini, Juan Pablo. Universidad de la República; Uruguay
Fil: Cabral, Pablo. Universidad de la República; Uruguay
Fil: Glisoni, Romina Julieta. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Nanobiotecnología. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Nanobiotecnología; Argentina
description Indocyanine green (ICG) is an FDA-approved near-infrared (NIR) dye used as a contrast agent for medical diagnosis in such techniques as image-guided surgery (IGS) and IGS-supported mapping for sentinel lymph node biopsy (SNLB). However, there are numerous disadvantages to its use in clinical applications: (i) self-aggregation in solution, (ii) poor targeting and (iii) short half-life in vivo, due to the rapid uptake by the liver. Herein, to overcome these obstacles, we utilized polymeric micelles (PMs) based on the amphiphilic linear and branched block poly(ethylene oxide)–poly(propylene oxide) (PEO–PPO) copolymers (Pluronic® and Tetronic®) for ICG stabilization, vehicleization and to directionally target breast cancer tissues. Because of their singular properties, PMs offer several advantages such as the ability to modify their surfaces with a variety of receptor-targeting ligands and their nano-scale size, which is suitable for taking advantage of the enhanced permeability and retention (EPR) effect for cancer diagnosis. In this work, we prepared ICG within pristine F127 and T1307 and their glucosylated derivatives (F127-Glu and T1307-Glu, respectively). These systems have a sub-30 nm-nanosized hydrodynamic diameter (19–27 nm), moderate negative Z-potentials (until −10 mV), and satisfactory stability in water even after lyophilisation and reconstitution, at 25 and 37 °C, respectively. Particularly, ICG within T1307-Glu PMs displayed maximum solubility and excellent encapsulation efficiency (100%), with a potentially large in vivo uptake according to high specificity and efficacious capture in lymph nodes (LNs) and tumors. All the results presented in this work, indicate that ICG-loaded PMs can potentially be used as image probe agents for IGS, SLNB and breast cancer imaging.
publishDate 2024
dc.date.none.fl_str_mv 2024-02
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/261026
Lecot, Nicole; Fernández Lomónaco, Marcelo; Cerecetto, Hugo; Gambini, Juan Pablo; Cabral, Pablo; et al.; Indocyanine green within glycosylated polymeric micelles as potential image agents to map sentinel lymph nodes and breast cancer; Royal Society of Chemistry; RSC Pharmaceutics; 1; 1; 2-2024; 57-67
2976-8713
CONICET Digital
CONICET
url http://hdl.handle.net/11336/261026
identifier_str_mv Lecot, Nicole; Fernández Lomónaco, Marcelo; Cerecetto, Hugo; Gambini, Juan Pablo; Cabral, Pablo; et al.; Indocyanine green within glycosylated polymeric micelles as potential image agents to map sentinel lymph nodes and breast cancer; Royal Society of Chemistry; RSC Pharmaceutics; 1; 1; 2-2024; 57-67
2976-8713
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://xlink.rsc.org/?DOI=D3PM00053B
info:eu-repo/semantics/altIdentifier/doi/10.1039/D3PM00053B
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Royal Society of Chemistry
publisher.none.fl_str_mv Royal Society of Chemistry
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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