Transcriptome dynamics of rooting zone and leaves during in vitro adventitious root formation in eucalyptus nitens

Autores
Ayala, Paula Gabriela; Acevedo, Raúl Maximiliano; Luna, Claudia Verónica; Rivarola, Maximo Lisandro; Acuña, Cintia Vanesa; Marcucci Poltri, Susana Noemi; González, Ana M.; Sansberro, Pedro Alfonso
Año de publicación
2022
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Wood properties and agronomic traits associated with fast growth and frost tolerance make Eucalyptus nitens a valuable forest alternative. However, the rapid age-related decline in the adventitious root (AR) formation (herein, meaning induction, initiation, and expression stages) limits its propagation. We analyzed transcriptomic profile variation in leaves and stem bases during AR induction of microcuttings to elucidate the molecular mechanisms involved in AR formation. In addition, we quantified expressions of candidate genes associated with recalcitrance. We delimited the ontogenic phases of root formation using histological techniques and Scarecrow and Short-Root expression quantification for RNA sequencing sample collection. We quantified the gene expressions associated with root meristem formation, auxin biosynthesis, perception, signaling, conjugation, and cytokinin signaling in shoots harvested from 2- to 36-month-old plants. After IBA treatment, 702 transcripts changed their expressions. Several were involved in hormone homeostasis and the signaling pathways that determine cell dedifferentiation, leading to root meristem formation. In part, the age-related decline in the rooting capacity is attributable to the increase in the ARR1 gene expression, which negatively affects auxin homeostasis. The analysis of the transcriptomic variation in the leaves and rooting zones provided profuse information: (1) To elucidate the auxin metabolism; (2) to understand the hormonal and signaling processes involved; (3) to collect data associated with their recalcitrance.
Instituto de Biotecnología
Fil: Ayala, Paula Gabriela. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias. Instituto de Botánica del Nordeste. Laboratorio de Biotecnología Aplicada y Genómica Funcional; Argentina
Fil: Ayala, Paula Gabriela. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Ayala, Paula Gabriela. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Concordia; Argentina
Fil: Acevedo, Raúl M. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias. Instituto de Botánica del Nordeste. Laboratorio de Biotecnología Aplicada y Genómica Funcional; Argentina
Fil: Acevedo, Raúl M. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Luna, Claudia Verónica. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias. Instituto de Botánica del Nordeste. Laboratorio de Biotecnología Aplicada y Genómica Funcional; Argentina
Fil: Luna, Claudia Verónica. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Rivarola, Maximo Lisandro. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina
Fil: Acuña, Cintia Vanesa. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina
Fil: Marcucci Poltri, Susana Noemi. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina
Fil: González, Ana M. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias. Instituto de Botánica del Nordeste. Laboratorio de Biotecnología Aplicada y Genómica Funcional; Argentina
Fil: González, Ana M. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Sansberro, Pedro Alfonso. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias. Instituto de Botánica del Nordeste. Laboratorio de Biotecnología Aplicada y Genómica Funcional; Argentina
Fil: Sansberro, Pedro Alfonso. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fuente
Plants 11 (23) : 3301 (Noviembre 2022)
Materia
Eucalyptus nitens
Raíces Adventicias
Transcriptomas
Auxinas
Adventitious Roots
Transcriptome
Auxins
In vitro
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/13704
Acceder
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network_name_str INTA Digital (INTA)
spelling Transcriptome dynamics of rooting zone and leaves during in vitro adventitious root formation in eucalyptus nitensAyala, Paula GabrielaAcevedo, Raúl MaximilianoLuna, Claudia VerónicaRivarola, Maximo LisandroAcuña, Cintia VanesaMarcucci Poltri, Susana NoemiGonzález, Ana M.Sansberro, Pedro AlfonsoEucalyptus nitensRaíces AdventiciasTranscriptomasAuxinasAdventitious RootsTranscriptomeAuxinsIn vitroWood properties and agronomic traits associated with fast growth and frost tolerance make Eucalyptus nitens a valuable forest alternative. However, the rapid age-related decline in the adventitious root (AR) formation (herein, meaning induction, initiation, and expression stages) limits its propagation. We analyzed transcriptomic profile variation in leaves and stem bases during AR induction of microcuttings to elucidate the molecular mechanisms involved in AR formation. In addition, we quantified expressions of candidate genes associated with recalcitrance. We delimited the ontogenic phases of root formation using histological techniques and Scarecrow and Short-Root expression quantification for RNA sequencing sample collection. We quantified the gene expressions associated with root meristem formation, auxin biosynthesis, perception, signaling, conjugation, and cytokinin signaling in shoots harvested from 2- to 36-month-old plants. After IBA treatment, 702 transcripts changed their expressions. Several were involved in hormone homeostasis and the signaling pathways that determine cell dedifferentiation, leading to root meristem formation. In part, the age-related decline in the rooting capacity is attributable to the increase in the ARR1 gene expression, which negatively affects auxin homeostasis. The analysis of the transcriptomic variation in the leaves and rooting zones provided profuse information: (1) To elucidate the auxin metabolism; (2) to understand the hormonal and signaling processes involved; (3) to collect data associated with their recalcitrance.Instituto de BiotecnologíaFil: Ayala, Paula Gabriela. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias. Instituto de Botánica del Nordeste. Laboratorio de Biotecnología Aplicada y Genómica Funcional; ArgentinaFil: Ayala, Paula Gabriela. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Ayala, Paula Gabriela. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Concordia; ArgentinaFil: Acevedo, Raúl M. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias. Instituto de Botánica del Nordeste. Laboratorio de Biotecnología Aplicada y Genómica Funcional; ArgentinaFil: Acevedo, Raúl M. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Luna, Claudia Verónica. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias. Instituto de Botánica del Nordeste. Laboratorio de Biotecnología Aplicada y Genómica Funcional; ArgentinaFil: Luna, Claudia Verónica. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Rivarola, Maximo Lisandro. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; ArgentinaFil: Acuña, Cintia Vanesa. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; ArgentinaFil: Marcucci Poltri, Susana Noemi. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; ArgentinaFil: González, Ana M. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias. Instituto de Botánica del Nordeste. Laboratorio de Biotecnología Aplicada y Genómica Funcional; ArgentinaFil: González, Ana M. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Sansberro, Pedro Alfonso. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias. Instituto de Botánica del Nordeste. Laboratorio de Biotecnología Aplicada y Genómica Funcional; ArgentinaFil: Sansberro, Pedro Alfonso. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaMDPI2022-12-26T13:34:28Z2022-12-26T13:34:28Z2022-11info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/13704https://www.mdpi.com/2223-7747/11/23/33012223-7747https://doi.org/10.3390/plants11233301Plants 11 (23) : 3301 (Noviembre 2022)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)2025-09-04T09:49:41Zoai:localhost:20.500.12123/13704instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-04 09:49:41.44INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Transcriptome dynamics of rooting zone and leaves during in vitro adventitious root formation in eucalyptus nitens
title Transcriptome dynamics of rooting zone and leaves during in vitro adventitious root formation in eucalyptus nitens
spellingShingle Transcriptome dynamics of rooting zone and leaves during in vitro adventitious root formation in eucalyptus nitens
Ayala, Paula Gabriela
Eucalyptus nitens
Raíces Adventicias
Transcriptomas
Auxinas
Adventitious Roots
Transcriptome
Auxins
In vitro
title_short Transcriptome dynamics of rooting zone and leaves during in vitro adventitious root formation in eucalyptus nitens
title_full Transcriptome dynamics of rooting zone and leaves during in vitro adventitious root formation in eucalyptus nitens
title_fullStr Transcriptome dynamics of rooting zone and leaves during in vitro adventitious root formation in eucalyptus nitens
title_full_unstemmed Transcriptome dynamics of rooting zone and leaves during in vitro adventitious root formation in eucalyptus nitens
title_sort Transcriptome dynamics of rooting zone and leaves during in vitro adventitious root formation in eucalyptus nitens
dc.creator.none.fl_str_mv Ayala, Paula Gabriela
Acevedo, Raúl Maximiliano
Luna, Claudia Verónica
Rivarola, Maximo Lisandro
Acuña, Cintia Vanesa
Marcucci Poltri, Susana Noemi
González, Ana M.
Sansberro, Pedro Alfonso
author Ayala, Paula Gabriela
author_facet Ayala, Paula Gabriela
Acevedo, Raúl Maximiliano
Luna, Claudia Verónica
Rivarola, Maximo Lisandro
Acuña, Cintia Vanesa
Marcucci Poltri, Susana Noemi
González, Ana M.
Sansberro, Pedro Alfonso
author_role author
author2 Acevedo, Raúl Maximiliano
Luna, Claudia Verónica
Rivarola, Maximo Lisandro
Acuña, Cintia Vanesa
Marcucci Poltri, Susana Noemi
González, Ana M.
Sansberro, Pedro Alfonso
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Eucalyptus nitens
Raíces Adventicias
Transcriptomas
Auxinas
Adventitious Roots
Transcriptome
Auxins
In vitro
topic Eucalyptus nitens
Raíces Adventicias
Transcriptomas
Auxinas
Adventitious Roots
Transcriptome
Auxins
In vitro
dc.description.none.fl_txt_mv Wood properties and agronomic traits associated with fast growth and frost tolerance make Eucalyptus nitens a valuable forest alternative. However, the rapid age-related decline in the adventitious root (AR) formation (herein, meaning induction, initiation, and expression stages) limits its propagation. We analyzed transcriptomic profile variation in leaves and stem bases during AR induction of microcuttings to elucidate the molecular mechanisms involved in AR formation. In addition, we quantified expressions of candidate genes associated with recalcitrance. We delimited the ontogenic phases of root formation using histological techniques and Scarecrow and Short-Root expression quantification for RNA sequencing sample collection. We quantified the gene expressions associated with root meristem formation, auxin biosynthesis, perception, signaling, conjugation, and cytokinin signaling in shoots harvested from 2- to 36-month-old plants. After IBA treatment, 702 transcripts changed their expressions. Several were involved in hormone homeostasis and the signaling pathways that determine cell dedifferentiation, leading to root meristem formation. In part, the age-related decline in the rooting capacity is attributable to the increase in the ARR1 gene expression, which negatively affects auxin homeostasis. The analysis of the transcriptomic variation in the leaves and rooting zones provided profuse information: (1) To elucidate the auxin metabolism; (2) to understand the hormonal and signaling processes involved; (3) to collect data associated with their recalcitrance.
Instituto de Biotecnología
Fil: Ayala, Paula Gabriela. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias. Instituto de Botánica del Nordeste. Laboratorio de Biotecnología Aplicada y Genómica Funcional; Argentina
Fil: Ayala, Paula Gabriela. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Ayala, Paula Gabriela. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Concordia; Argentina
Fil: Acevedo, Raúl M. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias. Instituto de Botánica del Nordeste. Laboratorio de Biotecnología Aplicada y Genómica Funcional; Argentina
Fil: Acevedo, Raúl M. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Luna, Claudia Verónica. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias. Instituto de Botánica del Nordeste. Laboratorio de Biotecnología Aplicada y Genómica Funcional; Argentina
Fil: Luna, Claudia Verónica. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Rivarola, Maximo Lisandro. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina
Fil: Acuña, Cintia Vanesa. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina
Fil: Marcucci Poltri, Susana Noemi. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina
Fil: González, Ana M. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias. Instituto de Botánica del Nordeste. Laboratorio de Biotecnología Aplicada y Genómica Funcional; Argentina
Fil: González, Ana M. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Sansberro, Pedro Alfonso. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias. Instituto de Botánica del Nordeste. Laboratorio de Biotecnología Aplicada y Genómica Funcional; Argentina
Fil: Sansberro, Pedro Alfonso. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
description Wood properties and agronomic traits associated with fast growth and frost tolerance make Eucalyptus nitens a valuable forest alternative. However, the rapid age-related decline in the adventitious root (AR) formation (herein, meaning induction, initiation, and expression stages) limits its propagation. We analyzed transcriptomic profile variation in leaves and stem bases during AR induction of microcuttings to elucidate the molecular mechanisms involved in AR formation. In addition, we quantified expressions of candidate genes associated with recalcitrance. We delimited the ontogenic phases of root formation using histological techniques and Scarecrow and Short-Root expression quantification for RNA sequencing sample collection. We quantified the gene expressions associated with root meristem formation, auxin biosynthesis, perception, signaling, conjugation, and cytokinin signaling in shoots harvested from 2- to 36-month-old plants. After IBA treatment, 702 transcripts changed their expressions. Several were involved in hormone homeostasis and the signaling pathways that determine cell dedifferentiation, leading to root meristem formation. In part, the age-related decline in the rooting capacity is attributable to the increase in the ARR1 gene expression, which negatively affects auxin homeostasis. The analysis of the transcriptomic variation in the leaves and rooting zones provided profuse information: (1) To elucidate the auxin metabolism; (2) to understand the hormonal and signaling processes involved; (3) to collect data associated with their recalcitrance.
publishDate 2022
dc.date.none.fl_str_mv 2022-12-26T13:34:28Z
2022-12-26T13:34:28Z
2022-11
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/13704
https://www.mdpi.com/2223-7747/11/23/3301
2223-7747
https://doi.org/10.3390/plants11233301
url http://hdl.handle.net/20.500.12123/13704
https://www.mdpi.com/2223-7747/11/23/3301
https://doi.org/10.3390/plants11233301
identifier_str_mv 2223-7747
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
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Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv Plants 11 (23) : 3301 (Noviembre 2022)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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