Effect of temperature on RNA silencing of a negative‐stranded RNA plant virus: Citrus psorosis virus

Autores
Velázquez, K.; Renovell, A.; Comellas, M.; Serra, P.; Garcia, Maria Laura; Pina, J. A.; Navarro, L.; Moreno, P.; Guerri, J.
Año de publicación
2010
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Citrus psorosis virus (CPsV), genus Ophiovirus, causes a bark scaling disease of citrus. CPsV virions are kinked filaments with three negative-stranded RNA molecules (vRNA) and a 48 kDa coat protein. The effect of temperature on symptom expression, virus accumulation and RNA silencing was examined in sweet orange seedlings (Citrus sinensis) graft-inoculated with three different CPsV isolates and grown in a glasshouse at 26⁄ 18_C or 32⁄26_C (day⁄ night). Most plants kept in the cooler glasshouse showed a shock reaction in the first flush with shoot necrosis, and then moderate to intense chlorotic flecking and spotting in young leaves, whereas plants incubated at 32⁄ 26_C did not exhibit shoot necrosis, and young leaf symptoms were milder. Virus titre estimated by ELISA and by northern and dot blot hybridization paralleled symptom intensity, with significantly higher virus accumulation in plants incubated at 26⁄ 18_C. The amount of CPsV-derived small RNAs (CPsV-sRNAs) slightly increased at 32⁄26_C, with the ratio of CPsV-sRNA⁄vRNA being higher at 32 ⁄ 26_C than at 26⁄18_C. These results suggest that (i) CPsV infection induces RNA silencing in citrus plants, (ii) symptom intensity is associated with virus accumulation, and (iii) temperature increase enhances the RNA silencing response of citrus plants and decreases virus accumulation.
Fil: Velázquez, K.. Institut Valencià D'investigacions Agràries.; España
Fil: Renovell, A.. Institut Valencià D'investigacions Agràries.; España
Fil: Comellas, M.. Institut Valencià D'investigacions Agràries.; España
Fil: Serra, P.. Institut Valencià D'investigacions Agràries.; España
Fil: Garcia, Maria Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; Argentina
Fil: Pina, J. A.. Institut Valencià D'investigacions Agràries.; España
Fil: Navarro, L.. Institut Valencià D'investigacions Agràries.; España
Fil: Moreno, P.. Institut Valencià D'investigacions Agràries.; España
Fil: Guerri, J.. Institut Valencià D'investigacions Agràries.; España
Materia
bark scaling citrus disease
CItrus psorosis virus
siRNA
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/277261
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/277261
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Effect of temperature on RNA silencing of a negative‐stranded RNA plant virus: Citrus psorosis virusVelázquez, K.Renovell, A.Comellas, M.Serra, P.Garcia, Maria LauraPina, J. A.Navarro, L.Moreno, P.Guerri, J.bark scaling citrus diseaseCItrus psorosis virussiRNAhttps://purl.org/becyt/ford/4.4https://purl.org/becyt/ford/4Citrus psorosis virus (CPsV), genus Ophiovirus, causes a bark scaling disease of citrus. CPsV virions are kinked filaments with three negative-stranded RNA molecules (vRNA) and a 48 kDa coat protein. The effect of temperature on symptom expression, virus accumulation and RNA silencing was examined in sweet orange seedlings (Citrus sinensis) graft-inoculated with three different CPsV isolates and grown in a glasshouse at 26⁄ 18_C or 32⁄26_C (day⁄ night). Most plants kept in the cooler glasshouse showed a shock reaction in the first flush with shoot necrosis, and then moderate to intense chlorotic flecking and spotting in young leaves, whereas plants incubated at 32⁄ 26_C did not exhibit shoot necrosis, and young leaf symptoms were milder. Virus titre estimated by ELISA and by northern and dot blot hybridization paralleled symptom intensity, with significantly higher virus accumulation in plants incubated at 26⁄ 18_C. The amount of CPsV-derived small RNAs (CPsV-sRNAs) slightly increased at 32⁄26_C, with the ratio of CPsV-sRNA⁄vRNA being higher at 32 ⁄ 26_C than at 26⁄18_C. These results suggest that (i) CPsV infection induces RNA silencing in citrus plants, (ii) symptom intensity is associated with virus accumulation, and (iii) temperature increase enhances the RNA silencing response of citrus plants and decreases virus accumulation.Fil: Velázquez, K.. Institut Valencià D'investigacions Agràries.; EspañaFil: Renovell, A.. Institut Valencià D'investigacions Agràries.; EspañaFil: Comellas, M.. Institut Valencià D'investigacions Agràries.; EspañaFil: Serra, P.. Institut Valencià D'investigacions Agràries.; EspañaFil: Garcia, Maria Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; ArgentinaFil: Pina, J. A.. Institut Valencià D'investigacions Agràries.; EspañaFil: Navarro, L.. Institut Valencià D'investigacions Agràries.; EspañaFil: Moreno, P.. Institut Valencià D'investigacions Agràries.; EspañaFil: Guerri, J.. Institut Valencià D'investigacions Agràries.; EspañaWiley Blackwell Publishing, Inc2010-04info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/277261Velázquez, K.; Renovell, A.; Comellas, M.; Serra, P.; Garcia, Maria Laura; et al.; Effect of temperature on RNA silencing of a negative‐stranded RNA plant virus: Citrus psorosis virus; Wiley Blackwell Publishing, Inc; Plant Pathology; 59; 5; 4-2010; 982-9900032-0862CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://bsppjournals.onlinelibrary.wiley.com/doi/10.1111/j.1365-3059.2010.02315.xinfo:eu-repo/semantics/altIdentifier/doi/10.1111/j.1365-3059.2010.02315.xinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-12-23T14:23:24Zoai:ri.conicet.gov.ar:11336/277261instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-12-23 14:23:24.632CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Effect of temperature on RNA silencing of a negative‐stranded RNA plant virus: Citrus psorosis virus
title Effect of temperature on RNA silencing of a negative‐stranded RNA plant virus: Citrus psorosis virus
spellingShingle Effect of temperature on RNA silencing of a negative‐stranded RNA plant virus: Citrus psorosis virus
Velázquez, K.
bark scaling citrus disease
CItrus psorosis virus
siRNA
title_short Effect of temperature on RNA silencing of a negative‐stranded RNA plant virus: Citrus psorosis virus
title_full Effect of temperature on RNA silencing of a negative‐stranded RNA plant virus: Citrus psorosis virus
title_fullStr Effect of temperature on RNA silencing of a negative‐stranded RNA plant virus: Citrus psorosis virus
title_full_unstemmed Effect of temperature on RNA silencing of a negative‐stranded RNA plant virus: Citrus psorosis virus
title_sort Effect of temperature on RNA silencing of a negative‐stranded RNA plant virus: Citrus psorosis virus
dc.creator.none.fl_str_mv Velázquez, K.
Renovell, A.
Comellas, M.
Serra, P.
Garcia, Maria Laura
Pina, J. A.
Navarro, L.
Moreno, P.
Guerri, J.
author Velázquez, K.
author_facet Velázquez, K.
Renovell, A.
Comellas, M.
Serra, P.
Garcia, Maria Laura
Pina, J. A.
Navarro, L.
Moreno, P.
Guerri, J.
author_role author
author2 Renovell, A.
Comellas, M.
Serra, P.
Garcia, Maria Laura
Pina, J. A.
Navarro, L.
Moreno, P.
Guerri, J.
author2_role author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv bark scaling citrus disease
CItrus psorosis virus
siRNA
topic bark scaling citrus disease
CItrus psorosis virus
siRNA
purl_subject.fl_str_mv https://purl.org/becyt/ford/4.4
https://purl.org/becyt/ford/4
dc.description.none.fl_txt_mv Citrus psorosis virus (CPsV), genus Ophiovirus, causes a bark scaling disease of citrus. CPsV virions are kinked filaments with three negative-stranded RNA molecules (vRNA) and a 48 kDa coat protein. The effect of temperature on symptom expression, virus accumulation and RNA silencing was examined in sweet orange seedlings (Citrus sinensis) graft-inoculated with three different CPsV isolates and grown in a glasshouse at 26⁄ 18_C or 32⁄26_C (day⁄ night). Most plants kept in the cooler glasshouse showed a shock reaction in the first flush with shoot necrosis, and then moderate to intense chlorotic flecking and spotting in young leaves, whereas plants incubated at 32⁄ 26_C did not exhibit shoot necrosis, and young leaf symptoms were milder. Virus titre estimated by ELISA and by northern and dot blot hybridization paralleled symptom intensity, with significantly higher virus accumulation in plants incubated at 26⁄ 18_C. The amount of CPsV-derived small RNAs (CPsV-sRNAs) slightly increased at 32⁄26_C, with the ratio of CPsV-sRNA⁄vRNA being higher at 32 ⁄ 26_C than at 26⁄18_C. These results suggest that (i) CPsV infection induces RNA silencing in citrus plants, (ii) symptom intensity is associated with virus accumulation, and (iii) temperature increase enhances the RNA silencing response of citrus plants and decreases virus accumulation.
Fil: Velázquez, K.. Institut Valencià D'investigacions Agràries.; España
Fil: Renovell, A.. Institut Valencià D'investigacions Agràries.; España
Fil: Comellas, M.. Institut Valencià D'investigacions Agràries.; España
Fil: Serra, P.. Institut Valencià D'investigacions Agràries.; España
Fil: Garcia, Maria Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; Argentina
Fil: Pina, J. A.. Institut Valencià D'investigacions Agràries.; España
Fil: Navarro, L.. Institut Valencià D'investigacions Agràries.; España
Fil: Moreno, P.. Institut Valencià D'investigacions Agràries.; España
Fil: Guerri, J.. Institut Valencià D'investigacions Agràries.; España
description Citrus psorosis virus (CPsV), genus Ophiovirus, causes a bark scaling disease of citrus. CPsV virions are kinked filaments with three negative-stranded RNA molecules (vRNA) and a 48 kDa coat protein. The effect of temperature on symptom expression, virus accumulation and RNA silencing was examined in sweet orange seedlings (Citrus sinensis) graft-inoculated with three different CPsV isolates and grown in a glasshouse at 26⁄ 18_C or 32⁄26_C (day⁄ night). Most plants kept in the cooler glasshouse showed a shock reaction in the first flush with shoot necrosis, and then moderate to intense chlorotic flecking and spotting in young leaves, whereas plants incubated at 32⁄ 26_C did not exhibit shoot necrosis, and young leaf symptoms were milder. Virus titre estimated by ELISA and by northern and dot blot hybridization paralleled symptom intensity, with significantly higher virus accumulation in plants incubated at 26⁄ 18_C. The amount of CPsV-derived small RNAs (CPsV-sRNAs) slightly increased at 32⁄26_C, with the ratio of CPsV-sRNA⁄vRNA being higher at 32 ⁄ 26_C than at 26⁄18_C. These results suggest that (i) CPsV infection induces RNA silencing in citrus plants, (ii) symptom intensity is associated with virus accumulation, and (iii) temperature increase enhances the RNA silencing response of citrus plants and decreases virus accumulation.
publishDate 2010
dc.date.none.fl_str_mv 2010-04
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/277261
Velázquez, K.; Renovell, A.; Comellas, M.; Serra, P.; Garcia, Maria Laura; et al.; Effect of temperature on RNA silencing of a negative‐stranded RNA plant virus: Citrus psorosis virus; Wiley Blackwell Publishing, Inc; Plant Pathology; 59; 5; 4-2010; 982-990
0032-0862
CONICET Digital
CONICET
url http://hdl.handle.net/11336/277261
identifier_str_mv Velázquez, K.; Renovell, A.; Comellas, M.; Serra, P.; Garcia, Maria Laura; et al.; Effect of temperature on RNA silencing of a negative‐stranded RNA plant virus: Citrus psorosis virus; Wiley Blackwell Publishing, Inc; Plant Pathology; 59; 5; 4-2010; 982-990
0032-0862
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://bsppjournals.onlinelibrary.wiley.com/doi/10.1111/j.1365-3059.2010.02315.x
info:eu-repo/semantics/altIdentifier/doi/10.1111/j.1365-3059.2010.02315.x
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Wiley Blackwell Publishing, Inc
publisher.none.fl_str_mv Wiley Blackwell Publishing, Inc
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 12.952241

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