Citrus psorosis virus 24K protein interacts with citrus miRNA precursors, affects their processing and subsequent miRNA accumulation and target expression

Autores
Reyes Martinez, Carina Andrea; Ocolotobiche, Eliana Evelina; Marmisollé, Facundo Ernesto; Robles Luna, Gabriel; Borniego, María Belén; Bazinni, Ariel Alejandro; Asurmendi, Sebastian; Garcia, Maria Laura
Año de publicación
2015
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Sweet orange (Citrus sinensis), one of the most important fruit crops worldwide, may suffer from disease symptoms induced by virus infections, thus resulting in dramatic economic losses. Here we show that the infection of sweet orange plants with two isolates of Citrus psorosis virus (CPsV) expressing different symptomatology alters the accumulation of a set of endogenous microRNAs (miRNAs). Within these miRNAs, miR156, miR167 and miR171 were the most downregulated with almost a 3?]fold reduction in the infected samples. This downregulation led to a concomitant upregulation of some of their targets, such as Squamosa promoter binding protein like 9 and 13 as well as Scarecrow?]like 6. The processing of miRNA precursors, pre?]miR156 and pre?]miR171, in sweet orange seems to be affected by the virus. For instance, the virus infection increases the level of unprocessed precursors, which is accompanied by the concomitant decrease of mature species accumulation. MiR156a primary transcript accumulation remained unaltered, thus strongly suggesting a processing deregulation for this transcript. The co?]immunoprecipitation of viral 24K protein with pre?]miR156a or pre?]miR171a suggests that the alteration in the processing of these precursors might be due to a direct or indirect interaction with this particular viral protein. This result is also consistent with the nuclear localization of both miRNA precursors and CPsV 24K protein. This study contributes to the understanding of the manner in which virus can alter host regulatory mechanisms, particularly miRNA biogenesis and target expression.
Fil: Reyes Martinez, Carina Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; Argentina
Fil: Ocolotobiche, Eliana Evelina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; Argentina
Fil: Marmisollé, Facundo Ernesto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; Argentina
Fil: Robles Luna, Gabriel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; Argentina
Fil: Borniego, María Belén. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; Argentina
Fil: Bazinni, Ariel Alejandro. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Virología; Argentina
Fil: Asurmendi, Sebastian. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Biotecnología; Argentina
Fil: Garcia, Maria Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; Argentina
Materia
Citrus Psorosis Virus
Mirnas
Symptons
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/41744
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/41744
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Citrus psorosis virus 24K protein interacts with citrus miRNA precursors, affects their processing and subsequent miRNA accumulation and target expressionReyes Martinez, Carina AndreaOcolotobiche, Eliana EvelinaMarmisollé, Facundo ErnestoRobles Luna, GabrielBorniego, María BelénBazinni, Ariel AlejandroAsurmendi, SebastianGarcia, Maria LauraCitrus Psorosis VirusMirnasSymptonshttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Sweet orange (Citrus sinensis), one of the most important fruit crops worldwide, may suffer from disease symptoms induced by virus infections, thus resulting in dramatic economic losses. Here we show that the infection of sweet orange plants with two isolates of Citrus psorosis virus (CPsV) expressing different symptomatology alters the accumulation of a set of endogenous microRNAs (miRNAs). Within these miRNAs, miR156, miR167 and miR171 were the most downregulated with almost a 3?]fold reduction in the infected samples. This downregulation led to a concomitant upregulation of some of their targets, such as Squamosa promoter binding protein like 9 and 13 as well as Scarecrow?]like 6. The processing of miRNA precursors, pre?]miR156 and pre?]miR171, in sweet orange seems to be affected by the virus. For instance, the virus infection increases the level of unprocessed precursors, which is accompanied by the concomitant decrease of mature species accumulation. MiR156a primary transcript accumulation remained unaltered, thus strongly suggesting a processing deregulation for this transcript. The co?]immunoprecipitation of viral 24K protein with pre?]miR156a or pre?]miR171a suggests that the alteration in the processing of these precursors might be due to a direct or indirect interaction with this particular viral protein. This result is also consistent with the nuclear localization of both miRNA precursors and CPsV 24K protein. This study contributes to the understanding of the manner in which virus can alter host regulatory mechanisms, particularly miRNA biogenesis and target expression.Fil: Reyes Martinez, Carina Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; ArgentinaFil: Ocolotobiche, Eliana Evelina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; ArgentinaFil: Marmisollé, Facundo Ernesto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; ArgentinaFil: Robles Luna, Gabriel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; ArgentinaFil: Borniego, María Belén. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; ArgentinaFil: Bazinni, Ariel Alejandro. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Virología; ArgentinaFil: Asurmendi, Sebastian. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Biotecnología; ArgentinaFil: Garcia, Maria Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; ArgentinaWiley Blackwell Publishing, Inc2015-05info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/41744Reyes Martinez, Carina Andrea; Ocolotobiche, Eliana Evelina; Marmisollé, Facundo Ernesto; Robles Luna, Gabriel; Borniego, María Belén; et al.; Citrus psorosis virus 24K protein interacts with citrus miRNA precursors, affects their processing and subsequent miRNA accumulation and target expression; Wiley Blackwell Publishing, Inc; Molecular Plant Pathology; 17; 3; 5-2015; 317-3291464-6722CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1111/mpp.12282info:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/abs/10.1111/mpp.12282info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:47:23Zoai:ri.conicet.gov.ar:11336/41744instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:47:24.194CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Citrus psorosis virus 24K protein interacts with citrus miRNA precursors, affects their processing and subsequent miRNA accumulation and target expression
title Citrus psorosis virus 24K protein interacts with citrus miRNA precursors, affects their processing and subsequent miRNA accumulation and target expression
spellingShingle Citrus psorosis virus 24K protein interacts with citrus miRNA precursors, affects their processing and subsequent miRNA accumulation and target expression
Reyes Martinez, Carina Andrea
Citrus Psorosis Virus
Mirnas
Symptons
title_short Citrus psorosis virus 24K protein interacts with citrus miRNA precursors, affects their processing and subsequent miRNA accumulation and target expression
title_full Citrus psorosis virus 24K protein interacts with citrus miRNA precursors, affects their processing and subsequent miRNA accumulation and target expression
title_fullStr Citrus psorosis virus 24K protein interacts with citrus miRNA precursors, affects their processing and subsequent miRNA accumulation and target expression
title_full_unstemmed Citrus psorosis virus 24K protein interacts with citrus miRNA precursors, affects their processing and subsequent miRNA accumulation and target expression
title_sort Citrus psorosis virus 24K protein interacts with citrus miRNA precursors, affects their processing and subsequent miRNA accumulation and target expression
dc.creator.none.fl_str_mv Reyes Martinez, Carina Andrea
Ocolotobiche, Eliana Evelina
Marmisollé, Facundo Ernesto
Robles Luna, Gabriel
Borniego, María Belén
Bazinni, Ariel Alejandro
Asurmendi, Sebastian
Garcia, Maria Laura
author Reyes Martinez, Carina Andrea
author_facet Reyes Martinez, Carina Andrea
Ocolotobiche, Eliana Evelina
Marmisollé, Facundo Ernesto
Robles Luna, Gabriel
Borniego, María Belén
Bazinni, Ariel Alejandro
Asurmendi, Sebastian
Garcia, Maria Laura
author_role author
author2 Ocolotobiche, Eliana Evelina
Marmisollé, Facundo Ernesto
Robles Luna, Gabriel
Borniego, María Belén
Bazinni, Ariel Alejandro
Asurmendi, Sebastian
Garcia, Maria Laura
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Citrus Psorosis Virus
Mirnas
Symptons
topic Citrus Psorosis Virus
Mirnas
Symptons
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Sweet orange (Citrus sinensis), one of the most important fruit crops worldwide, may suffer from disease symptoms induced by virus infections, thus resulting in dramatic economic losses. Here we show that the infection of sweet orange plants with two isolates of Citrus psorosis virus (CPsV) expressing different symptomatology alters the accumulation of a set of endogenous microRNAs (miRNAs). Within these miRNAs, miR156, miR167 and miR171 were the most downregulated with almost a 3?]fold reduction in the infected samples. This downregulation led to a concomitant upregulation of some of their targets, such as Squamosa promoter binding protein like 9 and 13 as well as Scarecrow?]like 6. The processing of miRNA precursors, pre?]miR156 and pre?]miR171, in sweet orange seems to be affected by the virus. For instance, the virus infection increases the level of unprocessed precursors, which is accompanied by the concomitant decrease of mature species accumulation. MiR156a primary transcript accumulation remained unaltered, thus strongly suggesting a processing deregulation for this transcript. The co?]immunoprecipitation of viral 24K protein with pre?]miR156a or pre?]miR171a suggests that the alteration in the processing of these precursors might be due to a direct or indirect interaction with this particular viral protein. This result is also consistent with the nuclear localization of both miRNA precursors and CPsV 24K protein. This study contributes to the understanding of the manner in which virus can alter host regulatory mechanisms, particularly miRNA biogenesis and target expression.
Fil: Reyes Martinez, Carina Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; Argentina
Fil: Ocolotobiche, Eliana Evelina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; Argentina
Fil: Marmisollé, Facundo Ernesto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; Argentina
Fil: Robles Luna, Gabriel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; Argentina
Fil: Borniego, María Belén. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; Argentina
Fil: Bazinni, Ariel Alejandro. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Virología; Argentina
Fil: Asurmendi, Sebastian. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Biotecnología; Argentina
Fil: Garcia, Maria Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; Argentina
description Sweet orange (Citrus sinensis), one of the most important fruit crops worldwide, may suffer from disease symptoms induced by virus infections, thus resulting in dramatic economic losses. Here we show that the infection of sweet orange plants with two isolates of Citrus psorosis virus (CPsV) expressing different symptomatology alters the accumulation of a set of endogenous microRNAs (miRNAs). Within these miRNAs, miR156, miR167 and miR171 were the most downregulated with almost a 3?]fold reduction in the infected samples. This downregulation led to a concomitant upregulation of some of their targets, such as Squamosa promoter binding protein like 9 and 13 as well as Scarecrow?]like 6. The processing of miRNA precursors, pre?]miR156 and pre?]miR171, in sweet orange seems to be affected by the virus. For instance, the virus infection increases the level of unprocessed precursors, which is accompanied by the concomitant decrease of mature species accumulation. MiR156a primary transcript accumulation remained unaltered, thus strongly suggesting a processing deregulation for this transcript. The co?]immunoprecipitation of viral 24K protein with pre?]miR156a or pre?]miR171a suggests that the alteration in the processing of these precursors might be due to a direct or indirect interaction with this particular viral protein. This result is also consistent with the nuclear localization of both miRNA precursors and CPsV 24K protein. This study contributes to the understanding of the manner in which virus can alter host regulatory mechanisms, particularly miRNA biogenesis and target expression.
publishDate 2015
dc.date.none.fl_str_mv 2015-05
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/41744
Reyes Martinez, Carina Andrea; Ocolotobiche, Eliana Evelina; Marmisollé, Facundo Ernesto; Robles Luna, Gabriel; Borniego, María Belén; et al.; Citrus psorosis virus 24K protein interacts with citrus miRNA precursors, affects their processing and subsequent miRNA accumulation and target expression; Wiley Blackwell Publishing, Inc; Molecular Plant Pathology; 17; 3; 5-2015; 317-329
1464-6722
CONICET Digital
CONICET
url http://hdl.handle.net/11336/41744
identifier_str_mv Reyes Martinez, Carina Andrea; Ocolotobiche, Eliana Evelina; Marmisollé, Facundo Ernesto; Robles Luna, Gabriel; Borniego, María Belén; et al.; Citrus psorosis virus 24K protein interacts with citrus miRNA precursors, affects their processing and subsequent miRNA accumulation and target expression; Wiley Blackwell Publishing, Inc; Molecular Plant Pathology; 17; 3; 5-2015; 317-329
1464-6722
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1111/mpp.12282
info:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/abs/10.1111/mpp.12282
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Wiley Blackwell Publishing, Inc
publisher.none.fl_str_mv Wiley Blackwell Publishing, Inc
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
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instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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