Generation of Sweet Orange Transgenic Lines and Evaluation of Citrus psorosis virus ‐derived Resistance against Psorosis A and Psorosis B

Autores
Reyes Martinez, Carina Andrea; Zanek, Maria Cecilia; Velázquez, Karelia; Costa, Norma; Plata, Maria Inés; Garcia, Maria Laura
Año de publicación
2011
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Citrus psorosis is a widespread serious disease of citrus caused by Citrus psorosis virus (CPsV). In Argentina and Uruguay, this disease is spread by an unknown vector and there is no natural resistance or tolerance to the disease. There are two types of psorosis, described according to the symptoms observed in citrus trees; psorosis A and psorosis B. Psorosis A protects against the severe effects of the more aggressive type psorosis B. We have applied pathogen-derived resistance to create a defence mechanism against this virus disease. Sweet orange transgenic lines were obtained containing three different genes of CPsV (54k, 48k and 24k genes) taken from a psorosis A isolate (CPV-4). Fourteen lines were selected containing 1, 2 or 3 copies of the transgenes and evaluated for their acquired resistance against psorosis A (CPV 4 from USA) and psorosis B (CPsV 189-34 from Argentina) isolates. These lines were susceptible to both isolates when graft-infected, although one of the lines carrying the cp gene (CP-96 line) containing two copies of the transgene and expressing a low level of the coat protein showed a delay in symptom expression when inoculated with the psorosis B isolate.
Fil: Reyes Martinez, Carina Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; Argentina
Fil: Zanek, Maria Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; Argentina
Fil: Velázquez, Karelia. Institut Valencià D'investigacions Agràries.; España
Fil: Costa, Norma. Estación Experimental Agropecuaria Concordia; Argentina
Fil: Plata, Maria Inés. Estación Experimental Agropecuaria Concordia; Argentina
Fil: Garcia, Maria Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; Argentina
Materia
citrus transgenic plants
citrus psorosis virus
ophiovirus
psorosis A psorosis B
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/278099
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/278099
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Generation of Sweet Orange Transgenic Lines and Evaluation of Citrus psorosis virus ‐derived Resistance against Psorosis A and Psorosis BReyes Martinez, Carina AndreaZanek, Maria CeciliaVelázquez, KareliaCosta, NormaPlata, Maria InésGarcia, Maria Lauracitrus transgenic plantscitrus psorosis virusophioviruspsorosis A psorosis Bhttps://purl.org/becyt/ford/4.4https://purl.org/becyt/ford/4Citrus psorosis is a widespread serious disease of citrus caused by Citrus psorosis virus (CPsV). In Argentina and Uruguay, this disease is spread by an unknown vector and there is no natural resistance or tolerance to the disease. There are two types of psorosis, described according to the symptoms observed in citrus trees; psorosis A and psorosis B. Psorosis A protects against the severe effects of the more aggressive type psorosis B. We have applied pathogen-derived resistance to create a defence mechanism against this virus disease. Sweet orange transgenic lines were obtained containing three different genes of CPsV (54k, 48k and 24k genes) taken from a psorosis A isolate (CPV-4). Fourteen lines were selected containing 1, 2 or 3 copies of the transgenes and evaluated for their acquired resistance against psorosis A (CPV 4 from USA) and psorosis B (CPsV 189-34 from Argentina) isolates. These lines were susceptible to both isolates when graft-infected, although one of the lines carrying the cp gene (CP-96 line) containing two copies of the transgene and expressing a low level of the coat protein showed a delay in symptom expression when inoculated with the psorosis B isolate.Fil: Reyes Martinez, Carina Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; ArgentinaFil: Zanek, Maria Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; ArgentinaFil: Velázquez, Karelia. Institut Valencià D'investigacions Agràries.; EspañaFil: Costa, Norma. Estación Experimental Agropecuaria Concordia; ArgentinaFil: Plata, Maria Inés. Estación Experimental Agropecuaria Concordia; ArgentinaFil: Garcia, Maria Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; ArgentinaWiley Blackwell Publishing, Inc2011-04info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/278099Reyes Martinez, Carina Andrea; Zanek, Maria Cecilia; Velázquez, Karelia; Costa, Norma; Plata, Maria Inés; et al.; Generation of Sweet Orange Transgenic Lines and Evaluation of Citrus psorosis virus ‐derived Resistance against Psorosis A and Psorosis B; Wiley Blackwell Publishing, Inc; Journal of Phytopathology-Phytopathologische Zeitschrift; 159; 7-8; 4-2011; 531-5370931-1785CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/10.1111/j.1439-0434.2011.01800.xinfo:eu-repo/semantics/altIdentifier/doi/10.1111/j.1439-0434.2011.01800.xinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-12-23T13:27:00Zoai:ri.conicet.gov.ar:11336/278099instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-12-23 13:27:00.623CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Generation of Sweet Orange Transgenic Lines and Evaluation of Citrus psorosis virus ‐derived Resistance against Psorosis A and Psorosis B
title Generation of Sweet Orange Transgenic Lines and Evaluation of Citrus psorosis virus ‐derived Resistance against Psorosis A and Psorosis B
spellingShingle Generation of Sweet Orange Transgenic Lines and Evaluation of Citrus psorosis virus ‐derived Resistance against Psorosis A and Psorosis B
Reyes Martinez, Carina Andrea
citrus transgenic plants
citrus psorosis virus
ophiovirus
psorosis A psorosis B
title_short Generation of Sweet Orange Transgenic Lines and Evaluation of Citrus psorosis virus ‐derived Resistance against Psorosis A and Psorosis B
title_full Generation of Sweet Orange Transgenic Lines and Evaluation of Citrus psorosis virus ‐derived Resistance against Psorosis A and Psorosis B
title_fullStr Generation of Sweet Orange Transgenic Lines and Evaluation of Citrus psorosis virus ‐derived Resistance against Psorosis A and Psorosis B
title_full_unstemmed Generation of Sweet Orange Transgenic Lines and Evaluation of Citrus psorosis virus ‐derived Resistance against Psorosis A and Psorosis B
title_sort Generation of Sweet Orange Transgenic Lines and Evaluation of Citrus psorosis virus ‐derived Resistance against Psorosis A and Psorosis B
dc.creator.none.fl_str_mv Reyes Martinez, Carina Andrea
Zanek, Maria Cecilia
Velázquez, Karelia
Costa, Norma
Plata, Maria Inés
Garcia, Maria Laura
author Reyes Martinez, Carina Andrea
author_facet Reyes Martinez, Carina Andrea
Zanek, Maria Cecilia
Velázquez, Karelia
Costa, Norma
Plata, Maria Inés
Garcia, Maria Laura
author_role author
author2 Zanek, Maria Cecilia
Velázquez, Karelia
Costa, Norma
Plata, Maria Inés
Garcia, Maria Laura
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv citrus transgenic plants
citrus psorosis virus
ophiovirus
psorosis A psorosis B
topic citrus transgenic plants
citrus psorosis virus
ophiovirus
psorosis A psorosis B
purl_subject.fl_str_mv https://purl.org/becyt/ford/4.4
https://purl.org/becyt/ford/4
dc.description.none.fl_txt_mv Citrus psorosis is a widespread serious disease of citrus caused by Citrus psorosis virus (CPsV). In Argentina and Uruguay, this disease is spread by an unknown vector and there is no natural resistance or tolerance to the disease. There are two types of psorosis, described according to the symptoms observed in citrus trees; psorosis A and psorosis B. Psorosis A protects against the severe effects of the more aggressive type psorosis B. We have applied pathogen-derived resistance to create a defence mechanism against this virus disease. Sweet orange transgenic lines were obtained containing three different genes of CPsV (54k, 48k and 24k genes) taken from a psorosis A isolate (CPV-4). Fourteen lines were selected containing 1, 2 or 3 copies of the transgenes and evaluated for their acquired resistance against psorosis A (CPV 4 from USA) and psorosis B (CPsV 189-34 from Argentina) isolates. These lines were susceptible to both isolates when graft-infected, although one of the lines carrying the cp gene (CP-96 line) containing two copies of the transgene and expressing a low level of the coat protein showed a delay in symptom expression when inoculated with the psorosis B isolate.
Fil: Reyes Martinez, Carina Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; Argentina
Fil: Zanek, Maria Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; Argentina
Fil: Velázquez, Karelia. Institut Valencià D'investigacions Agràries.; España
Fil: Costa, Norma. Estación Experimental Agropecuaria Concordia; Argentina
Fil: Plata, Maria Inés. Estación Experimental Agropecuaria Concordia; Argentina
Fil: Garcia, Maria Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; Argentina
description Citrus psorosis is a widespread serious disease of citrus caused by Citrus psorosis virus (CPsV). In Argentina and Uruguay, this disease is spread by an unknown vector and there is no natural resistance or tolerance to the disease. There are two types of psorosis, described according to the symptoms observed in citrus trees; psorosis A and psorosis B. Psorosis A protects against the severe effects of the more aggressive type psorosis B. We have applied pathogen-derived resistance to create a defence mechanism against this virus disease. Sweet orange transgenic lines were obtained containing three different genes of CPsV (54k, 48k and 24k genes) taken from a psorosis A isolate (CPV-4). Fourteen lines were selected containing 1, 2 or 3 copies of the transgenes and evaluated for their acquired resistance against psorosis A (CPV 4 from USA) and psorosis B (CPsV 189-34 from Argentina) isolates. These lines were susceptible to both isolates when graft-infected, although one of the lines carrying the cp gene (CP-96 line) containing two copies of the transgene and expressing a low level of the coat protein showed a delay in symptom expression when inoculated with the psorosis B isolate.
publishDate 2011
dc.date.none.fl_str_mv 2011-04
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/278099
Reyes Martinez, Carina Andrea; Zanek, Maria Cecilia; Velázquez, Karelia; Costa, Norma; Plata, Maria Inés; et al.; Generation of Sweet Orange Transgenic Lines and Evaluation of Citrus psorosis virus ‐derived Resistance against Psorosis A and Psorosis B; Wiley Blackwell Publishing, Inc; Journal of Phytopathology-Phytopathologische Zeitschrift; 159; 7-8; 4-2011; 531-537
0931-1785
CONICET Digital
CONICET
url http://hdl.handle.net/11336/278099
identifier_str_mv Reyes Martinez, Carina Andrea; Zanek, Maria Cecilia; Velázquez, Karelia; Costa, Norma; Plata, Maria Inés; et al.; Generation of Sweet Orange Transgenic Lines and Evaluation of Citrus psorosis virus ‐derived Resistance against Psorosis A and Psorosis B; Wiley Blackwell Publishing, Inc; Journal of Phytopathology-Phytopathologische Zeitschrift; 159; 7-8; 4-2011; 531-537
0931-1785
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/10.1111/j.1439-0434.2011.01800.x
info:eu-repo/semantics/altIdentifier/doi/10.1111/j.1439-0434.2011.01800.x
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Wiley Blackwell Publishing, Inc
publisher.none.fl_str_mv Wiley Blackwell Publishing, Inc
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 12.952241

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