Polysaccharide localization in the sporophyte cell wall of Adenocystis utricularis (Ectocarpales s.l., Phaeophyceae)

Autores
Ponce, Nora Marta Andrea; Leonardi, Patricia Ines; Flores, Maria Lujan; Stortz, Carlos Arturo; Rodríguez, María Cecilia
Año de publicación
2007
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The chemical composition of the sporophyte cell wall of Adenocystis utricularis was examined by using a number of histochemical techniques for the localization of polysaccharides, including labeling with a fluorescent alginate probe. Histochemical staining allowed confirmation of a low alginate/fucoidan ratio in the cell wall indicated by exhaustive extraction of polysaccharides from the algal thalli. Alginate was located in the inner portion of cell walls. “Egg-box” structure of alginate in inner cortical cells may be related to the mechanism of algal growth. Abundance of fucoidan was confirmed by extraction on semithin sections and staining. Weak staining on nonextracted material was in agreement with previously proposed blockage of sulfate groups in these polysaccharides.
Fil: Ponce, Nora Marta Andrea. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Orgánica; Argentina
Fil: Leonardi, Patricia Ines. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; Argentina
Fil: Flores, Maria Lujan. Universidad Nacional de la Patagonia "san Juan Bosco". Facultad de Cs.naturales - Sede Comodoro. Departamento de Farmacia. Cátedra de Farmacognosia; Argentina
Fil: Stortz, Carlos Arturo. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Orgánica; Argentina
Fil: Rodríguez, María Cecilia. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Biodiversidad y Biología Experimental; Argentina
Materia
Adenocystis Utricularis
Alginate, Fucoidan
Histochemistry
Cell Wall Polysaccharides
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/30432
Acceder
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network_name_str CONICET Digital (CONICET)
spelling Polysaccharide localization in the sporophyte cell wall of Adenocystis utricularis (Ectocarpales s.l., Phaeophyceae)Ponce, Nora Marta AndreaLeonardi, Patricia InesFlores, Maria LujanStortz, Carlos ArturoRodríguez, María CeciliaAdenocystis UtricularisAlginate, FucoidanHistochemistryCell Wall Polysaccharideshttps://purl.org/becyt/ford/3.3https://purl.org/becyt/ford/3The chemical composition of the sporophyte cell wall of Adenocystis utricularis was examined by using a number of histochemical techniques for the localization of polysaccharides, including labeling with a fluorescent alginate probe. Histochemical staining allowed confirmation of a low alginate/fucoidan ratio in the cell wall indicated by exhaustive extraction of polysaccharides from the algal thalli. Alginate was located in the inner portion of cell walls. “Egg-box” structure of alginate in inner cortical cells may be related to the mechanism of algal growth. Abundance of fucoidan was confirmed by extraction on semithin sections and staining. Weak staining on nonextracted material was in agreement with previously proposed blockage of sulfate groups in these polysaccharides.Fil: Ponce, Nora Marta Andrea. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Orgánica; ArgentinaFil: Leonardi, Patricia Ines. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; ArgentinaFil: Flores, Maria Lujan. Universidad Nacional de la Patagonia "san Juan Bosco". Facultad de Cs.naturales - Sede Comodoro. Departamento de Farmacia. Cátedra de Farmacognosia; ArgentinaFil: Stortz, Carlos Arturo. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Orgánica; ArgentinaFil: Rodríguez, María Cecilia. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Biodiversidad y Biología Experimental; ArgentinaInt Phycological Soc2007-11info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/30432Ponce, Nora Marta Andrea; Leonardi, Patricia Ines; Flores, Maria Lujan; Stortz, Carlos Arturo; Rodríguez, María Cecilia; Polysaccharide localization in the sporophyte cell wall of Adenocystis utricularis (Ectocarpales s.l., Phaeophyceae); Int Phycological Soc; Phycologia; 46; 6; 11-2007; 675-6790031-8884CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://www.phycologia.org/doi/abs/10.2216/06-102.1?code=iphy-siteinfo:eu-repo/semantics/altIdentifier/doi/10.2216/06-102.1info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:56:57Zoai:ri.conicet.gov.ar:11336/30432instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:56:58.014CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Polysaccharide localization in the sporophyte cell wall of Adenocystis utricularis (Ectocarpales s.l., Phaeophyceae)
title Polysaccharide localization in the sporophyte cell wall of Adenocystis utricularis (Ectocarpales s.l., Phaeophyceae)
spellingShingle Polysaccharide localization in the sporophyte cell wall of Adenocystis utricularis (Ectocarpales s.l., Phaeophyceae)
Ponce, Nora Marta Andrea
Adenocystis Utricularis
Alginate, Fucoidan
Histochemistry
Cell Wall Polysaccharides
title_short Polysaccharide localization in the sporophyte cell wall of Adenocystis utricularis (Ectocarpales s.l., Phaeophyceae)
title_full Polysaccharide localization in the sporophyte cell wall of Adenocystis utricularis (Ectocarpales s.l., Phaeophyceae)
title_fullStr Polysaccharide localization in the sporophyte cell wall of Adenocystis utricularis (Ectocarpales s.l., Phaeophyceae)
title_full_unstemmed Polysaccharide localization in the sporophyte cell wall of Adenocystis utricularis (Ectocarpales s.l., Phaeophyceae)
title_sort Polysaccharide localization in the sporophyte cell wall of Adenocystis utricularis (Ectocarpales s.l., Phaeophyceae)
dc.creator.none.fl_str_mv Ponce, Nora Marta Andrea
Leonardi, Patricia Ines
Flores, Maria Lujan
Stortz, Carlos Arturo
Rodríguez, María Cecilia
author Ponce, Nora Marta Andrea
author_facet Ponce, Nora Marta Andrea
Leonardi, Patricia Ines
Flores, Maria Lujan
Stortz, Carlos Arturo
Rodríguez, María Cecilia
author_role author
author2 Leonardi, Patricia Ines
Flores, Maria Lujan
Stortz, Carlos Arturo
Rodríguez, María Cecilia
author2_role author
author
author
author
dc.subject.none.fl_str_mv Adenocystis Utricularis
Alginate, Fucoidan
Histochemistry
Cell Wall Polysaccharides
topic Adenocystis Utricularis
Alginate, Fucoidan
Histochemistry
Cell Wall Polysaccharides
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.3
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv The chemical composition of the sporophyte cell wall of Adenocystis utricularis was examined by using a number of histochemical techniques for the localization of polysaccharides, including labeling with a fluorescent alginate probe. Histochemical staining allowed confirmation of a low alginate/fucoidan ratio in the cell wall indicated by exhaustive extraction of polysaccharides from the algal thalli. Alginate was located in the inner portion of cell walls. “Egg-box” structure of alginate in inner cortical cells may be related to the mechanism of algal growth. Abundance of fucoidan was confirmed by extraction on semithin sections and staining. Weak staining on nonextracted material was in agreement with previously proposed blockage of sulfate groups in these polysaccharides.
Fil: Ponce, Nora Marta Andrea. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Orgánica; Argentina
Fil: Leonardi, Patricia Ines. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; Argentina
Fil: Flores, Maria Lujan. Universidad Nacional de la Patagonia "san Juan Bosco". Facultad de Cs.naturales - Sede Comodoro. Departamento de Farmacia. Cátedra de Farmacognosia; Argentina
Fil: Stortz, Carlos Arturo. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Orgánica; Argentina
Fil: Rodríguez, María Cecilia. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Biodiversidad y Biología Experimental; Argentina
description The chemical composition of the sporophyte cell wall of Adenocystis utricularis was examined by using a number of histochemical techniques for the localization of polysaccharides, including labeling with a fluorescent alginate probe. Histochemical staining allowed confirmation of a low alginate/fucoidan ratio in the cell wall indicated by exhaustive extraction of polysaccharides from the algal thalli. Alginate was located in the inner portion of cell walls. “Egg-box” structure of alginate in inner cortical cells may be related to the mechanism of algal growth. Abundance of fucoidan was confirmed by extraction on semithin sections and staining. Weak staining on nonextracted material was in agreement with previously proposed blockage of sulfate groups in these polysaccharides.
publishDate 2007
dc.date.none.fl_str_mv 2007-11
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/30432
Ponce, Nora Marta Andrea; Leonardi, Patricia Ines; Flores, Maria Lujan; Stortz, Carlos Arturo; Rodríguez, María Cecilia; Polysaccharide localization in the sporophyte cell wall of Adenocystis utricularis (Ectocarpales s.l., Phaeophyceae); Int Phycological Soc; Phycologia; 46; 6; 11-2007; 675-679
0031-8884
CONICET Digital
CONICET
url http://hdl.handle.net/11336/30432
identifier_str_mv Ponce, Nora Marta Andrea; Leonardi, Patricia Ines; Flores, Maria Lujan; Stortz, Carlos Arturo; Rodríguez, María Cecilia; Polysaccharide localization in the sporophyte cell wall of Adenocystis utricularis (Ectocarpales s.l., Phaeophyceae); Int Phycological Soc; Phycologia; 46; 6; 11-2007; 675-679
0031-8884
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://www.phycologia.org/doi/abs/10.2216/06-102.1?code=iphy-site
info:eu-repo/semantics/altIdentifier/doi/10.2216/06-102.1
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Int Phycological Soc
publisher.none.fl_str_mv Int Phycological Soc
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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