Degradation of Metsulfuron Methyl by Agaricus blazei Murrill Spent Compost Enzymes

Autores
González Matute, Ramiro; Figlas, Norma Débora; Mockel, Gabriela Carolina; Curvetto, Nestor Raul
Año de publicación
2012
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Metsulfuron methyl (MM) is an herbicide used in cereal crops. The white rot mushroom Agaricus blazei Murrill is an important edible and medicinal mushroom reported to be a major laccase producer, a lignindegrading enzyme with low substrate specificity. A search for assaying the potential use of A. blazei spent mushroom compost (SMC) as a remediation tool for cleaning MM polluted soils was carried out. A phytotoxic dose of this herbicide was separately incubated with two enzyme preparations obtained from the SMC after the second mushroom fruiting flush; the phytotoxicity of the resulting reaction mixtures was then assayed by using a plantlet growing test with Brassica napus L. Thus, the crude enzyme SMC extract preparation (I) or the partially purified enzyme SMC extract (II) and their dilutions, 1:10 and 1:100, were mixed withMM(5 × 10−3 ppm final concentration) and incubated at 25◦C for 24, 48, 72, and 96 h. Plantlets separately exposed for 72 and 96 h to the resulting reaction mixtures betweenMMand those enzyme preparations showed a highly significant increase in their hypocotyl length with respect to plantlets exposed to MM alone. It was thus demonstrated the ability that complex enzyme fractions present in A. blazei SMC have to degrade MM during the right incubation time to compounds with no or lower phytotoxicity than this herbicide.
Fil: González Matute, Ramiro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; Argentina. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas; Argentina
Fil: Figlas, Norma Débora. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; Argentina. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas; Argentina
Fil: Mockel, Gabriela Carolina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; Argentina
Fil: Curvetto, Nestor Raul. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; Argentina
Materia
Agaricus brasiliensis
Bioremediation
Phytotoxicity
Lignin-modifying enzymes
White rot fungi
Laccase
Spent mushroom compost
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/247479
Acceder
id CONICETDig_6664b25cfc799bcc4807b6f4f3a86c8d
oai_identifier_str oai:ri.conicet.gov.ar:11336/247479
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Degradation of Metsulfuron Methyl by Agaricus blazei Murrill Spent Compost EnzymesGonzález Matute, RamiroFiglas, Norma DéboraMockel, Gabriela CarolinaCurvetto, Nestor RaulAgaricus brasiliensisBioremediationPhytotoxicityLignin-modifying enzymesWhite rot fungiLaccaseSpent mushroom composthttps://purl.org/becyt/ford/4.4https://purl.org/becyt/ford/4Metsulfuron methyl (MM) is an herbicide used in cereal crops. The white rot mushroom Agaricus blazei Murrill is an important edible and medicinal mushroom reported to be a major laccase producer, a lignindegrading enzyme with low substrate specificity. A search for assaying the potential use of A. blazei spent mushroom compost (SMC) as a remediation tool for cleaning MM polluted soils was carried out. A phytotoxic dose of this herbicide was separately incubated with two enzyme preparations obtained from the SMC after the second mushroom fruiting flush; the phytotoxicity of the resulting reaction mixtures was then assayed by using a plantlet growing test with Brassica napus L. Thus, the crude enzyme SMC extract preparation (I) or the partially purified enzyme SMC extract (II) and their dilutions, 1:10 and 1:100, were mixed withMM(5 × 10−3 ppm final concentration) and incubated at 25◦C for 24, 48, 72, and 96 h. Plantlets separately exposed for 72 and 96 h to the resulting reaction mixtures betweenMMand those enzyme preparations showed a highly significant increase in their hypocotyl length with respect to plantlets exposed to MM alone. It was thus demonstrated the ability that complex enzyme fractions present in A. blazei SMC have to degrade MM during the right incubation time to compounds with no or lower phytotoxicity than this herbicide.Fil: González Matute, Ramiro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; Argentina. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas; ArgentinaFil: Figlas, Norma Débora. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; Argentina. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas; ArgentinaFil: Mockel, Gabriela Carolina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; ArgentinaFil: Curvetto, Nestor Raul. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; ArgentinaTaylor & Francis2012-02-29info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/247479González Matute, Ramiro; Figlas, Norma Débora; Mockel, Gabriela Carolina; Curvetto, Nestor Raul; Degradation of Metsulfuron Methyl by Agaricus blazei Murrill Spent Compost Enzymes; Taylor & Francis; Bioremediation Journal; 16; 1; 29-2-2012; 31-371088-9868CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1080/10889868.2011.628353info:eu-repo/semantics/altIdentifier/url/https://www.tandfonline.com/doi/abs/10.1080/10889868.2011.628353info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:48:19Zoai:ri.conicet.gov.ar:11336/247479instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:48:19.343CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Degradation of Metsulfuron Methyl by Agaricus blazei Murrill Spent Compost Enzymes
title Degradation of Metsulfuron Methyl by Agaricus blazei Murrill Spent Compost Enzymes
spellingShingle Degradation of Metsulfuron Methyl by Agaricus blazei Murrill Spent Compost Enzymes
González Matute, Ramiro
Agaricus brasiliensis
Bioremediation
Phytotoxicity
Lignin-modifying enzymes
White rot fungi
Laccase
Spent mushroom compost
title_short Degradation of Metsulfuron Methyl by Agaricus blazei Murrill Spent Compost Enzymes
title_full Degradation of Metsulfuron Methyl by Agaricus blazei Murrill Spent Compost Enzymes
title_fullStr Degradation of Metsulfuron Methyl by Agaricus blazei Murrill Spent Compost Enzymes
title_full_unstemmed Degradation of Metsulfuron Methyl by Agaricus blazei Murrill Spent Compost Enzymes
title_sort Degradation of Metsulfuron Methyl by Agaricus blazei Murrill Spent Compost Enzymes
dc.creator.none.fl_str_mv González Matute, Ramiro
Figlas, Norma Débora
Mockel, Gabriela Carolina
Curvetto, Nestor Raul
author González Matute, Ramiro
author_facet González Matute, Ramiro
Figlas, Norma Débora
Mockel, Gabriela Carolina
Curvetto, Nestor Raul
author_role author
author2 Figlas, Norma Débora
Mockel, Gabriela Carolina
Curvetto, Nestor Raul
author2_role author
author
author
dc.subject.none.fl_str_mv Agaricus brasiliensis
Bioremediation
Phytotoxicity
Lignin-modifying enzymes
White rot fungi
Laccase
Spent mushroom compost
topic Agaricus brasiliensis
Bioremediation
Phytotoxicity
Lignin-modifying enzymes
White rot fungi
Laccase
Spent mushroom compost
purl_subject.fl_str_mv https://purl.org/becyt/ford/4.4
https://purl.org/becyt/ford/4
dc.description.none.fl_txt_mv Metsulfuron methyl (MM) is an herbicide used in cereal crops. The white rot mushroom Agaricus blazei Murrill is an important edible and medicinal mushroom reported to be a major laccase producer, a lignindegrading enzyme with low substrate specificity. A search for assaying the potential use of A. blazei spent mushroom compost (SMC) as a remediation tool for cleaning MM polluted soils was carried out. A phytotoxic dose of this herbicide was separately incubated with two enzyme preparations obtained from the SMC after the second mushroom fruiting flush; the phytotoxicity of the resulting reaction mixtures was then assayed by using a plantlet growing test with Brassica napus L. Thus, the crude enzyme SMC extract preparation (I) or the partially purified enzyme SMC extract (II) and their dilutions, 1:10 and 1:100, were mixed withMM(5 × 10−3 ppm final concentration) and incubated at 25◦C for 24, 48, 72, and 96 h. Plantlets separately exposed for 72 and 96 h to the resulting reaction mixtures betweenMMand those enzyme preparations showed a highly significant increase in their hypocotyl length with respect to plantlets exposed to MM alone. It was thus demonstrated the ability that complex enzyme fractions present in A. blazei SMC have to degrade MM during the right incubation time to compounds with no or lower phytotoxicity than this herbicide.
Fil: González Matute, Ramiro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; Argentina. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas; Argentina
Fil: Figlas, Norma Débora. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; Argentina. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas; Argentina
Fil: Mockel, Gabriela Carolina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; Argentina
Fil: Curvetto, Nestor Raul. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; Argentina
description Metsulfuron methyl (MM) is an herbicide used in cereal crops. The white rot mushroom Agaricus blazei Murrill is an important edible and medicinal mushroom reported to be a major laccase producer, a lignindegrading enzyme with low substrate specificity. A search for assaying the potential use of A. blazei spent mushroom compost (SMC) as a remediation tool for cleaning MM polluted soils was carried out. A phytotoxic dose of this herbicide was separately incubated with two enzyme preparations obtained from the SMC after the second mushroom fruiting flush; the phytotoxicity of the resulting reaction mixtures was then assayed by using a plantlet growing test with Brassica napus L. Thus, the crude enzyme SMC extract preparation (I) or the partially purified enzyme SMC extract (II) and their dilutions, 1:10 and 1:100, were mixed withMM(5 × 10−3 ppm final concentration) and incubated at 25◦C for 24, 48, 72, and 96 h. Plantlets separately exposed for 72 and 96 h to the resulting reaction mixtures betweenMMand those enzyme preparations showed a highly significant increase in their hypocotyl length with respect to plantlets exposed to MM alone. It was thus demonstrated the ability that complex enzyme fractions present in A. blazei SMC have to degrade MM during the right incubation time to compounds with no or lower phytotoxicity than this herbicide.
publishDate 2012
dc.date.none.fl_str_mv 2012-02-29
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/247479
González Matute, Ramiro; Figlas, Norma Débora; Mockel, Gabriela Carolina; Curvetto, Nestor Raul; Degradation of Metsulfuron Methyl by Agaricus blazei Murrill Spent Compost Enzymes; Taylor & Francis; Bioremediation Journal; 16; 1; 29-2-2012; 31-37
1088-9868
CONICET Digital
CONICET
url http://hdl.handle.net/11336/247479
identifier_str_mv González Matute, Ramiro; Figlas, Norma Débora; Mockel, Gabriela Carolina; Curvetto, Nestor Raul; Degradation of Metsulfuron Methyl by Agaricus blazei Murrill Spent Compost Enzymes; Taylor & Francis; Bioremediation Journal; 16; 1; 29-2-2012; 31-37
1088-9868
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1080/10889868.2011.628353
info:eu-repo/semantics/altIdentifier/url/https://www.tandfonline.com/doi/abs/10.1080/10889868.2011.628353
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Taylor & Francis
publisher.none.fl_str_mv Taylor & Francis
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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