Proving Geobacter biofilm connectivity with confocal Raman microscopy

Autores
Robuschi, Luciana; Tomba, Juan Pablo; Busalmen, Juan Pablo
Año de publicación
2016
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
We report here an . in-vivo, non-invasive, depth profiling study on an electrically-active biofilm by Confocal Raman Microscopy. Films based on . Geobacter sulfurreducens bacteria were grown onto an indium tin oxide (ITO) conducting glass, where the ITO piece plays a double role: it acts as a supporting polarized electrode for the bacteria population but also as a transparent window that gives direct access to the biofilm body. A confocal Raman microscope was used to examine the biofilm redox state at increasing distances from the ITO surface, upon variable polarization and under the presence or not of a chemical electron donor or acceptor. Resonant Raman effect allowed to selectively probe the redox response of biofilm cytochromes to the applied potential and chemicals, revealing internal connectivity details of the bacterial population. Full methodological details for this application case are given, while the importance of confocal Raman for the analysis of biological or electrochemically active systems in non-invasive way is highlighted.
Fil: Robuschi, Luciana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones en Ciencia y Tecnología de Materiales. Universidad Nacional de Mar del Plata. Facultad de Ingeniería. Instituto de Investigaciones en Ciencia y Tecnología de Materiales; Argentina
Fil: Tomba, Juan Pablo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones en Ciencia y Tecnología de Materiales. Universidad Nacional de Mar del Plata. Facultad de Ingeniería. Instituto de Investigaciones en Ciencia y Tecnología de Materiales; Argentina
Fil: Busalmen, Juan Pablo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones en Ciencia y Tecnología de Materiales. Universidad Nacional de Mar del Plata. Facultad de Ingeniería. Instituto de Investigaciones en Ciencia y Tecnología de Materiales; Argentina
Materia
Confocal Raman Microscopy
Geobacter Sulfurreducens
Biofilm
Redox State
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/34057
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/34057
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Proving Geobacter biofilm connectivity with confocal Raman microscopyRobuschi, LucianaTomba, Juan PabloBusalmen, Juan PabloConfocal Raman MicroscopyGeobacter SulfurreducensBiofilmRedox Statehttps://purl.org/becyt/ford/1.4https://purl.org/becyt/ford/1https://purl.org/becyt/ford/2.5https://purl.org/becyt/ford/2We report here an . in-vivo, non-invasive, depth profiling study on an electrically-active biofilm by Confocal Raman Microscopy. Films based on . Geobacter sulfurreducens bacteria were grown onto an indium tin oxide (ITO) conducting glass, where the ITO piece plays a double role: it acts as a supporting polarized electrode for the bacteria population but also as a transparent window that gives direct access to the biofilm body. A confocal Raman microscope was used to examine the biofilm redox state at increasing distances from the ITO surface, upon variable polarization and under the presence or not of a chemical electron donor or acceptor. Resonant Raman effect allowed to selectively probe the redox response of biofilm cytochromes to the applied potential and chemicals, revealing internal connectivity details of the bacterial population. Full methodological details for this application case are given, while the importance of confocal Raman for the analysis of biological or electrochemically active systems in non-invasive way is highlighted.Fil: Robuschi, Luciana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones en Ciencia y Tecnología de Materiales. Universidad Nacional de Mar del Plata. Facultad de Ingeniería. Instituto de Investigaciones en Ciencia y Tecnología de Materiales; ArgentinaFil: Tomba, Juan Pablo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones en Ciencia y Tecnología de Materiales. Universidad Nacional de Mar del Plata. Facultad de Ingeniería. Instituto de Investigaciones en Ciencia y Tecnología de Materiales; ArgentinaFil: Busalmen, Juan Pablo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones en Ciencia y Tecnología de Materiales. Universidad Nacional de Mar del Plata. Facultad de Ingeniería. Instituto de Investigaciones en Ciencia y Tecnología de Materiales; ArgentinaElsevier Science Sa2016-11-04info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/34057Robuschi, Luciana; Tomba, Juan Pablo; Busalmen, Juan Pablo; Proving Geobacter biofilm connectivity with confocal Raman microscopy; Elsevier Science Sa; Journal of Electroanalytical Chemistry; 793; 4-11-2016; 99-1031572-6657CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.jelechem.2016.11.005info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S1572665716306087?via%3Dihubinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T14:21:39Zoai:ri.conicet.gov.ar:11336/34057instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 14:21:39.699CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Proving Geobacter biofilm connectivity with confocal Raman microscopy
title Proving Geobacter biofilm connectivity with confocal Raman microscopy
spellingShingle Proving Geobacter biofilm connectivity with confocal Raman microscopy
Robuschi, Luciana
Confocal Raman Microscopy
Geobacter Sulfurreducens
Biofilm
Redox State
title_short Proving Geobacter biofilm connectivity with confocal Raman microscopy
title_full Proving Geobacter biofilm connectivity with confocal Raman microscopy
title_fullStr Proving Geobacter biofilm connectivity with confocal Raman microscopy
title_full_unstemmed Proving Geobacter biofilm connectivity with confocal Raman microscopy
title_sort Proving Geobacter biofilm connectivity with confocal Raman microscopy
dc.creator.none.fl_str_mv Robuschi, Luciana
Tomba, Juan Pablo
Busalmen, Juan Pablo
author Robuschi, Luciana
author_facet Robuschi, Luciana
Tomba, Juan Pablo
Busalmen, Juan Pablo
author_role author
author2 Tomba, Juan Pablo
Busalmen, Juan Pablo
author2_role author
author
dc.subject.none.fl_str_mv Confocal Raman Microscopy
Geobacter Sulfurreducens
Biofilm
Redox State
topic Confocal Raman Microscopy
Geobacter Sulfurreducens
Biofilm
Redox State
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.4
https://purl.org/becyt/ford/1
https://purl.org/becyt/ford/2.5
https://purl.org/becyt/ford/2
dc.description.none.fl_txt_mv We report here an . in-vivo, non-invasive, depth profiling study on an electrically-active biofilm by Confocal Raman Microscopy. Films based on . Geobacter sulfurreducens bacteria were grown onto an indium tin oxide (ITO) conducting glass, where the ITO piece plays a double role: it acts as a supporting polarized electrode for the bacteria population but also as a transparent window that gives direct access to the biofilm body. A confocal Raman microscope was used to examine the biofilm redox state at increasing distances from the ITO surface, upon variable polarization and under the presence or not of a chemical electron donor or acceptor. Resonant Raman effect allowed to selectively probe the redox response of biofilm cytochromes to the applied potential and chemicals, revealing internal connectivity details of the bacterial population. Full methodological details for this application case are given, while the importance of confocal Raman for the analysis of biological or electrochemically active systems in non-invasive way is highlighted.
Fil: Robuschi, Luciana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones en Ciencia y Tecnología de Materiales. Universidad Nacional de Mar del Plata. Facultad de Ingeniería. Instituto de Investigaciones en Ciencia y Tecnología de Materiales; Argentina
Fil: Tomba, Juan Pablo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones en Ciencia y Tecnología de Materiales. Universidad Nacional de Mar del Plata. Facultad de Ingeniería. Instituto de Investigaciones en Ciencia y Tecnología de Materiales; Argentina
Fil: Busalmen, Juan Pablo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones en Ciencia y Tecnología de Materiales. Universidad Nacional de Mar del Plata. Facultad de Ingeniería. Instituto de Investigaciones en Ciencia y Tecnología de Materiales; Argentina
description We report here an . in-vivo, non-invasive, depth profiling study on an electrically-active biofilm by Confocal Raman Microscopy. Films based on . Geobacter sulfurreducens bacteria were grown onto an indium tin oxide (ITO) conducting glass, where the ITO piece plays a double role: it acts as a supporting polarized electrode for the bacteria population but also as a transparent window that gives direct access to the biofilm body. A confocal Raman microscope was used to examine the biofilm redox state at increasing distances from the ITO surface, upon variable polarization and under the presence or not of a chemical electron donor or acceptor. Resonant Raman effect allowed to selectively probe the redox response of biofilm cytochromes to the applied potential and chemicals, revealing internal connectivity details of the bacterial population. Full methodological details for this application case are given, while the importance of confocal Raman for the analysis of biological or electrochemically active systems in non-invasive way is highlighted.
publishDate 2016
dc.date.none.fl_str_mv 2016-11-04
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/34057
Robuschi, Luciana; Tomba, Juan Pablo; Busalmen, Juan Pablo; Proving Geobacter biofilm connectivity with confocal Raman microscopy; Elsevier Science Sa; Journal of Electroanalytical Chemistry; 793; 4-11-2016; 99-103
1572-6657
CONICET Digital
CONICET
url http://hdl.handle.net/11336/34057
identifier_str_mv Robuschi, Luciana; Tomba, Juan Pablo; Busalmen, Juan Pablo; Proving Geobacter biofilm connectivity with confocal Raman microscopy; Elsevier Science Sa; Journal of Electroanalytical Chemistry; 793; 4-11-2016; 99-103
1572-6657
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1016/j.jelechem.2016.11.005
info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S1572665716306087?via%3Dihub
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Science Sa
publisher.none.fl_str_mv Elsevier Science Sa
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.22299

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