Transmission electron microscopy as a relevant tool in the characterization of hybrid nanostructures of Au Bio-Mineralization by Electroactive Bacteria.

Autores
Muñoz, Vanesa; Inchaurrondo, Joaquín; Busalmen, Juan Pablo; Ordoñez, Maria Victoria
Año de publicación
2020
Idioma
inglés
Tipo de recurso
documento de conferencia
Estado
versión publicada
Descripción
Geobacter sulfurreducens is an anaerobic gram(?) bacteria capable of using a wide range of electron acceptor, including insoluble Fe(III) (hydr)oxides, and anode electrodes, thus expelling electrons beyond cells limits. Outer membrane cytochromes (C-type) are the main redox proteins involved in external electron transfer.We have previously observed the capacity of Geobacter to use Au(III) ions as electron acceptor, thusforming AuNPs that help improve current production in electro active biofilms. In this work we goforward on the isolation and characterization of nanostructures synthetized by Geobacter cultures grown in batch. Au respiration was performed at standard growing conditions, with 20, 100 or 200 mM AuHCl4 for 1 week period. Soluble fraction containing gold NPs as well as biological material was then obtained and applied to a sucrose discontinuous gradient to achieve nanoparticle size separation. Both Au-containing cultures (previously fixed) and sucrose gradient fractions were placed in 200-mesh Formvar film copper grids and were studied by transmission electron microscopy in a JEOL JEM 2100 at 80 and 120 kV.Micrographs corresponding to Geobacter cells producing AuNPs revealed the localization of suchstructures mostly in contact with external membrane, forming cumulus at several spots (Figure 1).Cumulus formation correlate to initial Au(III) concentration added to the stationary grown cultures.Results obtained from fractioning showed that NPs separation from cellular debris was achieved finding smaller NPs (2-10 nm) in 30% sucrose fraction and larger NPs in 40 to 60% sucrose fraction, most of which have an spherical shape (Figure 2). Results indicate conjugates of cytochromes-AuNPs would form, thus obtaining hybrid nanostructures.
Fil: Muñoz, Vanesa. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones en Ciencia y Tecnología de Materiales. Universidad Nacional de Mar del Plata. Facultad de Ingeniería. Instituto de Investigaciones en Ciencia y Tecnología de Materiales; Argentina
Fil: Inchaurrondo, Joaquín. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones en Ciencia y Tecnología de Materiales. Universidad Nacional de Mar del Plata. Facultad de Ingeniería. Instituto de Investigaciones en Ciencia y Tecnología de Materiales; Argentina
Fil: Busalmen, Juan Pablo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones en Ciencia y Tecnología de Materiales. Universidad Nacional de Mar del Plata. Facultad de Ingeniería. Instituto de Investigaciones en Ciencia y Tecnología de Materiales; Argentina
Fil: Ordoñez, Maria Victoria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones en Ciencia y Tecnología de Materiales. Universidad Nacional de Mar del Plata. Facultad de Ingeniería. Instituto de Investigaciones en Ciencia y Tecnología de Materiales; Argentina
15th Interamerican Microscopy Congress CIASEM - SAMIC
Ciudad Autónoma de Buenos Aires
Argentina
Sociedades de Microscopía Electrónica
Materia
TRANSMISSION ELECTRON MICROSCOPY
AU - NANOPARTICLES
GEOBACTER SULFURREDUCENS
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/245230
Acceder
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network_acronym_str CONICETDig
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network_name_str CONICET Digital (CONICET)
spelling Transmission electron microscopy as a relevant tool in the characterization of hybrid nanostructures of Au Bio-Mineralization by Electroactive Bacteria.Muñoz, VanesaInchaurrondo, JoaquínBusalmen, Juan PabloOrdoñez, Maria VictoriaTRANSMISSION ELECTRON MICROSCOPYAU - NANOPARTICLESGEOBACTER SULFURREDUCENShttps://purl.org/becyt/ford/2.10https://purl.org/becyt/ford/2Geobacter sulfurreducens is an anaerobic gram(?) bacteria capable of using a wide range of electron acceptor, including insoluble Fe(III) (hydr)oxides, and anode electrodes, thus expelling electrons beyond cells limits. Outer membrane cytochromes (C-type) are the main redox proteins involved in external electron transfer.We have previously observed the capacity of Geobacter to use Au(III) ions as electron acceptor, thusforming AuNPs that help improve current production in electro active biofilms. In this work we goforward on the isolation and characterization of nanostructures synthetized by Geobacter cultures grown in batch. Au respiration was performed at standard growing conditions, with 20, 100 or 200 mM AuHCl4 for 1 week period. Soluble fraction containing gold NPs as well as biological material was then obtained and applied to a sucrose discontinuous gradient to achieve nanoparticle size separation. Both Au-containing cultures (previously fixed) and sucrose gradient fractions were placed in 200-mesh Formvar film copper grids and were studied by transmission electron microscopy in a JEOL JEM 2100 at 80 and 120 kV.Micrographs corresponding to Geobacter cells producing AuNPs revealed the localization of suchstructures mostly in contact with external membrane, forming cumulus at several spots (Figure 1).Cumulus formation correlate to initial Au(III) concentration added to the stationary grown cultures.Results obtained from fractioning showed that NPs separation from cellular debris was achieved finding smaller NPs (2-10 nm) in 30% sucrose fraction and larger NPs in 40 to 60% sucrose fraction, most of which have an spherical shape (Figure 2). Results indicate conjugates of cytochromes-AuNPs would form, thus obtaining hybrid nanostructures.Fil: Muñoz, Vanesa. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones en Ciencia y Tecnología de Materiales. Universidad Nacional de Mar del Plata. Facultad de Ingeniería. Instituto de Investigaciones en Ciencia y Tecnología de Materiales; ArgentinaFil: Inchaurrondo, Joaquín. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones en Ciencia y Tecnología de Materiales. Universidad Nacional de Mar del Plata. Facultad de Ingeniería. Instituto de Investigaciones en Ciencia y Tecnología de Materiales; ArgentinaFil: Busalmen, Juan Pablo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones en Ciencia y Tecnología de Materiales. Universidad Nacional de Mar del Plata. Facultad de Ingeniería. Instituto de Investigaciones en Ciencia y Tecnología de Materiales; ArgentinaFil: Ordoñez, Maria Victoria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones en Ciencia y Tecnología de Materiales. Universidad Nacional de Mar del Plata. Facultad de Ingeniería. Instituto de Investigaciones en Ciencia y Tecnología de Materiales; Argentina15th Interamerican Microscopy Congress CIASEM - SAMICCiudad Autónoma de Buenos AiresArgentinaSociedades de Microscopía ElectrónicaCambridge University Press2020info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectCongresoJournalhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/245230Transmission electron microscopy as a relevant tool in the characterization of hybrid nanostructures of Au Bio-Mineralization by Electroactive Bacteria.; 15th Interamerican Microscopy Congress CIASEM - SAMIC; Ciudad Autónoma de Buenos Aires; Argentina; 2019; 189-1901431-9276CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.cambridge.org/core/journals/microscopy-and-microanalysis/article/transmission-electron-microscopy-as-a-relevant-tool-in-the-characterization-of-hybrid-nanostructures-of-au-biomineralization-by-electroactive-bacteria/364EC1CB212C87CCE6DB39D9C305FDA7info:eu-repo/semantics/altIdentifier/doi/10.1017/S1431927620001166Internacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:46:01Zoai:ri.conicet.gov.ar:11336/245230instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:46:02.018CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Transmission electron microscopy as a relevant tool in the characterization of hybrid nanostructures of Au Bio-Mineralization by Electroactive Bacteria.
title Transmission electron microscopy as a relevant tool in the characterization of hybrid nanostructures of Au Bio-Mineralization by Electroactive Bacteria.
spellingShingle Transmission electron microscopy as a relevant tool in the characterization of hybrid nanostructures of Au Bio-Mineralization by Electroactive Bacteria.
Muñoz, Vanesa
TRANSMISSION ELECTRON MICROSCOPY
AU - NANOPARTICLES
GEOBACTER SULFURREDUCENS
title_short Transmission electron microscopy as a relevant tool in the characterization of hybrid nanostructures of Au Bio-Mineralization by Electroactive Bacteria.
title_full Transmission electron microscopy as a relevant tool in the characterization of hybrid nanostructures of Au Bio-Mineralization by Electroactive Bacteria.
title_fullStr Transmission electron microscopy as a relevant tool in the characterization of hybrid nanostructures of Au Bio-Mineralization by Electroactive Bacteria.
title_full_unstemmed Transmission electron microscopy as a relevant tool in the characterization of hybrid nanostructures of Au Bio-Mineralization by Electroactive Bacteria.
title_sort Transmission electron microscopy as a relevant tool in the characterization of hybrid nanostructures of Au Bio-Mineralization by Electroactive Bacteria.
dc.creator.none.fl_str_mv Muñoz, Vanesa
Inchaurrondo, Joaquín
Busalmen, Juan Pablo
Ordoñez, Maria Victoria
author Muñoz, Vanesa
author_facet Muñoz, Vanesa
Inchaurrondo, Joaquín
Busalmen, Juan Pablo
Ordoñez, Maria Victoria
author_role author
author2 Inchaurrondo, Joaquín
Busalmen, Juan Pablo
Ordoñez, Maria Victoria
author2_role author
author
author
dc.subject.none.fl_str_mv TRANSMISSION ELECTRON MICROSCOPY
AU - NANOPARTICLES
GEOBACTER SULFURREDUCENS
topic TRANSMISSION ELECTRON MICROSCOPY
AU - NANOPARTICLES
GEOBACTER SULFURREDUCENS
purl_subject.fl_str_mv https://purl.org/becyt/ford/2.10
https://purl.org/becyt/ford/2
dc.description.none.fl_txt_mv Geobacter sulfurreducens is an anaerobic gram(?) bacteria capable of using a wide range of electron acceptor, including insoluble Fe(III) (hydr)oxides, and anode electrodes, thus expelling electrons beyond cells limits. Outer membrane cytochromes (C-type) are the main redox proteins involved in external electron transfer.We have previously observed the capacity of Geobacter to use Au(III) ions as electron acceptor, thusforming AuNPs that help improve current production in electro active biofilms. In this work we goforward on the isolation and characterization of nanostructures synthetized by Geobacter cultures grown in batch. Au respiration was performed at standard growing conditions, with 20, 100 or 200 mM AuHCl4 for 1 week period. Soluble fraction containing gold NPs as well as biological material was then obtained and applied to a sucrose discontinuous gradient to achieve nanoparticle size separation. Both Au-containing cultures (previously fixed) and sucrose gradient fractions were placed in 200-mesh Formvar film copper grids and were studied by transmission electron microscopy in a JEOL JEM 2100 at 80 and 120 kV.Micrographs corresponding to Geobacter cells producing AuNPs revealed the localization of suchstructures mostly in contact with external membrane, forming cumulus at several spots (Figure 1).Cumulus formation correlate to initial Au(III) concentration added to the stationary grown cultures.Results obtained from fractioning showed that NPs separation from cellular debris was achieved finding smaller NPs (2-10 nm) in 30% sucrose fraction and larger NPs in 40 to 60% sucrose fraction, most of which have an spherical shape (Figure 2). Results indicate conjugates of cytochromes-AuNPs would form, thus obtaining hybrid nanostructures.
Fil: Muñoz, Vanesa. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones en Ciencia y Tecnología de Materiales. Universidad Nacional de Mar del Plata. Facultad de Ingeniería. Instituto de Investigaciones en Ciencia y Tecnología de Materiales; Argentina
Fil: Inchaurrondo, Joaquín. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones en Ciencia y Tecnología de Materiales. Universidad Nacional de Mar del Plata. Facultad de Ingeniería. Instituto de Investigaciones en Ciencia y Tecnología de Materiales; Argentina
Fil: Busalmen, Juan Pablo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones en Ciencia y Tecnología de Materiales. Universidad Nacional de Mar del Plata. Facultad de Ingeniería. Instituto de Investigaciones en Ciencia y Tecnología de Materiales; Argentina
Fil: Ordoñez, Maria Victoria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones en Ciencia y Tecnología de Materiales. Universidad Nacional de Mar del Plata. Facultad de Ingeniería. Instituto de Investigaciones en Ciencia y Tecnología de Materiales; Argentina
15th Interamerican Microscopy Congress CIASEM - SAMIC
Ciudad Autónoma de Buenos Aires
Argentina
Sociedades de Microscopía Electrónica
description Geobacter sulfurreducens is an anaerobic gram(?) bacteria capable of using a wide range of electron acceptor, including insoluble Fe(III) (hydr)oxides, and anode electrodes, thus expelling electrons beyond cells limits. Outer membrane cytochromes (C-type) are the main redox proteins involved in external electron transfer.We have previously observed the capacity of Geobacter to use Au(III) ions as electron acceptor, thusforming AuNPs that help improve current production in electro active biofilms. In this work we goforward on the isolation and characterization of nanostructures synthetized by Geobacter cultures grown in batch. Au respiration was performed at standard growing conditions, with 20, 100 or 200 mM AuHCl4 for 1 week period. Soluble fraction containing gold NPs as well as biological material was then obtained and applied to a sucrose discontinuous gradient to achieve nanoparticle size separation. Both Au-containing cultures (previously fixed) and sucrose gradient fractions were placed in 200-mesh Formvar film copper grids and were studied by transmission electron microscopy in a JEOL JEM 2100 at 80 and 120 kV.Micrographs corresponding to Geobacter cells producing AuNPs revealed the localization of suchstructures mostly in contact with external membrane, forming cumulus at several spots (Figure 1).Cumulus formation correlate to initial Au(III) concentration added to the stationary grown cultures.Results obtained from fractioning showed that NPs separation from cellular debris was achieved finding smaller NPs (2-10 nm) in 30% sucrose fraction and larger NPs in 40 to 60% sucrose fraction, most of which have an spherical shape (Figure 2). Results indicate conjugates of cytochromes-AuNPs would form, thus obtaining hybrid nanostructures.
publishDate 2020
dc.date.none.fl_str_mv 2020
dc.type.none.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/conferenceObject
Congreso
Journal
http://purl.org/coar/resource_type/c_5794
info:ar-repo/semantics/documentoDeConferencia
status_str publishedVersion
format conferenceObject
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/245230
Transmission electron microscopy as a relevant tool in the characterization of hybrid nanostructures of Au Bio-Mineralization by Electroactive Bacteria.; 15th Interamerican Microscopy Congress CIASEM - SAMIC; Ciudad Autónoma de Buenos Aires; Argentina; 2019; 189-190
1431-9276
CONICET Digital
CONICET
url http://hdl.handle.net/11336/245230
identifier_str_mv Transmission electron microscopy as a relevant tool in the characterization of hybrid nanostructures of Au Bio-Mineralization by Electroactive Bacteria.; 15th Interamerican Microscopy Congress CIASEM - SAMIC; Ciudad Autónoma de Buenos Aires; Argentina; 2019; 189-190
1431-9276
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.cambridge.org/core/journals/microscopy-and-microanalysis/article/transmission-electron-microscopy-as-a-relevant-tool-in-the-characterization-of-hybrid-nanostructures-of-au-biomineralization-by-electroactive-bacteria/364EC1CB212C87CCE6DB39D9C305FDA7
info:eu-repo/semantics/altIdentifier/doi/10.1017/S1431927620001166
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.coverage.none.fl_str_mv Internacional
dc.publisher.none.fl_str_mv Cambridge University Press
publisher.none.fl_str_mv Cambridge University Press
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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