Morphology, morphometry, histochemistry and lectin histochemistry of the vagina of the plains viscacha (Lagostomus maximus)

Autores
Flamini, Mirta Alicia; Díaz, Alcira Ofelia; Barbeito, Claudio Gustavo; Portiansky, Enrique Leo
Año de publicación
2012
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The aim of the present work was to describe the morphology of the vagina in Lagostomus maximus and to characterize its epithelial cells using morphometric and histochemical techniques (variations of PAS, Alcian blue and lectin histochemistry). Thirty-fi ve sexually mature adult females were captured in their natural environment during four periods of the year and their genital organs were dissected. The vaginal wall of the viscacha has three tunics: mucosa, muscularis and adventitia or serosa according to the region. The epithelium is stratifi ed in both cranial and caudal regions, but its characteristics vary depending on the physiological state. In anestrous, nonpregnant females have a stratifi ed epithelium of two to three cellular layers with columnar PAS-positive superfi cial mucous cells. During the follicular phase, the epithelium of the vagina is stratifi ed squamous and cornifi ed. Females at early, middle and term pregnancy have a columnar stratifi ed epithelium with mucous cells. Glycoproteins in the mucous cells were detected using PAS, PA ∗ S, KOH/PA ∗ /BH/PAS; and Alcian blue, pH 0.5, pH 1, pH 2.5 and 0.006 M). Lectin histochemistry showed that UEA-I and RCA-1 lectins reacted strongly or moderately with epithelial cells in all stages analyzed. These results indicate the presence of L-fucose and â -galactose. Binding with other lectins was variable.
Fil: Flamini, Mirta Alicia. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias; Argentina
Fil: Díaz, Alcira Ofelia. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales; Argentina
Fil: Barbeito, Claudio Gustavo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias; Argentina. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Departamento de Biología; Argentina
Fil: Portiansky, Enrique Leo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias; Argentina
Materia
Histochemistry
Histricomorph
Lectinhistochemistry
Mucous cells
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/272993
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/272993
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Morphology, morphometry, histochemistry and lectin histochemistry of the vagina of the plains viscacha (Lagostomus maximus)Flamini, Mirta AliciaDíaz, Alcira OfeliaBarbeito, Claudio GustavoPortiansky, Enrique LeoHistochemistryHistricomorphLectinhistochemistryMucous cellshttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1The aim of the present work was to describe the morphology of the vagina in Lagostomus maximus and to characterize its epithelial cells using morphometric and histochemical techniques (variations of PAS, Alcian blue and lectin histochemistry). Thirty-fi ve sexually mature adult females were captured in their natural environment during four periods of the year and their genital organs were dissected. The vaginal wall of the viscacha has three tunics: mucosa, muscularis and adventitia or serosa according to the region. The epithelium is stratifi ed in both cranial and caudal regions, but its characteristics vary depending on the physiological state. In anestrous, nonpregnant females have a stratifi ed epithelium of two to three cellular layers with columnar PAS-positive superfi cial mucous cells. During the follicular phase, the epithelium of the vagina is stratifi ed squamous and cornifi ed. Females at early, middle and term pregnancy have a columnar stratifi ed epithelium with mucous cells. Glycoproteins in the mucous cells were detected using PAS, PA ∗ S, KOH/PA ∗ /BH/PAS; and Alcian blue, pH 0.5, pH 1, pH 2.5 and 0.006 M). Lectin histochemistry showed that UEA-I and RCA-1 lectins reacted strongly or moderately with epithelial cells in all stages analyzed. These results indicate the presence of L-fucose and â -galactose. Binding with other lectins was variable.Fil: Flamini, Mirta Alicia. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias; ArgentinaFil: Díaz, Alcira Ofelia. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales; ArgentinaFil: Barbeito, Claudio Gustavo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias; Argentina. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Departamento de Biología; ArgentinaFil: Portiansky, Enrique Leo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias; ArgentinaInforma Healthcare2012-05info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/272993Flamini, Mirta Alicia; Díaz, Alcira Ofelia; Barbeito, Claudio Gustavo; Portiansky, Enrique Leo; Morphology, morphometry, histochemistry and lectin histochemistry of the vagina of the plains viscacha (Lagostomus maximus); Informa Healthcare; Biotechnic and Histochemistry; 87; 2; 5-2012; 81-941052-0295CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.tandfonline.com/doi/full/10.3109/10520295.2010.518497info:eu-repo/semantics/altIdentifier/doi/10.3109/10520295.2010.518497info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T15:12:01Zoai:ri.conicet.gov.ar:11336/272993instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 15:12:02.244CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Morphology, morphometry, histochemistry and lectin histochemistry of the vagina of the plains viscacha (Lagostomus maximus)
title Morphology, morphometry, histochemistry and lectin histochemistry of the vagina of the plains viscacha (Lagostomus maximus)
spellingShingle Morphology, morphometry, histochemistry and lectin histochemistry of the vagina of the plains viscacha (Lagostomus maximus)
Flamini, Mirta Alicia
Histochemistry
Histricomorph
Lectinhistochemistry
Mucous cells
title_short Morphology, morphometry, histochemistry and lectin histochemistry of the vagina of the plains viscacha (Lagostomus maximus)
title_full Morphology, morphometry, histochemistry and lectin histochemistry of the vagina of the plains viscacha (Lagostomus maximus)
title_fullStr Morphology, morphometry, histochemistry and lectin histochemistry of the vagina of the plains viscacha (Lagostomus maximus)
title_full_unstemmed Morphology, morphometry, histochemistry and lectin histochemistry of the vagina of the plains viscacha (Lagostomus maximus)
title_sort Morphology, morphometry, histochemistry and lectin histochemistry of the vagina of the plains viscacha (Lagostomus maximus)
dc.creator.none.fl_str_mv Flamini, Mirta Alicia
Díaz, Alcira Ofelia
Barbeito, Claudio Gustavo
Portiansky, Enrique Leo
author Flamini, Mirta Alicia
author_facet Flamini, Mirta Alicia
Díaz, Alcira Ofelia
Barbeito, Claudio Gustavo
Portiansky, Enrique Leo
author_role author
author2 Díaz, Alcira Ofelia
Barbeito, Claudio Gustavo
Portiansky, Enrique Leo
author2_role author
author
author
dc.subject.none.fl_str_mv Histochemistry
Histricomorph
Lectinhistochemistry
Mucous cells
topic Histochemistry
Histricomorph
Lectinhistochemistry
Mucous cells
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv The aim of the present work was to describe the morphology of the vagina in Lagostomus maximus and to characterize its epithelial cells using morphometric and histochemical techniques (variations of PAS, Alcian blue and lectin histochemistry). Thirty-fi ve sexually mature adult females were captured in their natural environment during four periods of the year and their genital organs were dissected. The vaginal wall of the viscacha has three tunics: mucosa, muscularis and adventitia or serosa according to the region. The epithelium is stratifi ed in both cranial and caudal regions, but its characteristics vary depending on the physiological state. In anestrous, nonpregnant females have a stratifi ed epithelium of two to three cellular layers with columnar PAS-positive superfi cial mucous cells. During the follicular phase, the epithelium of the vagina is stratifi ed squamous and cornifi ed. Females at early, middle and term pregnancy have a columnar stratifi ed epithelium with mucous cells. Glycoproteins in the mucous cells were detected using PAS, PA ∗ S, KOH/PA ∗ /BH/PAS; and Alcian blue, pH 0.5, pH 1, pH 2.5 and 0.006 M). Lectin histochemistry showed that UEA-I and RCA-1 lectins reacted strongly or moderately with epithelial cells in all stages analyzed. These results indicate the presence of L-fucose and â -galactose. Binding with other lectins was variable.
Fil: Flamini, Mirta Alicia. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias; Argentina
Fil: Díaz, Alcira Ofelia. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales; Argentina
Fil: Barbeito, Claudio Gustavo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias; Argentina. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Departamento de Biología; Argentina
Fil: Portiansky, Enrique Leo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias; Argentina
description The aim of the present work was to describe the morphology of the vagina in Lagostomus maximus and to characterize its epithelial cells using morphometric and histochemical techniques (variations of PAS, Alcian blue and lectin histochemistry). Thirty-fi ve sexually mature adult females were captured in their natural environment during four periods of the year and their genital organs were dissected. The vaginal wall of the viscacha has three tunics: mucosa, muscularis and adventitia or serosa according to the region. The epithelium is stratifi ed in both cranial and caudal regions, but its characteristics vary depending on the physiological state. In anestrous, nonpregnant females have a stratifi ed epithelium of two to three cellular layers with columnar PAS-positive superfi cial mucous cells. During the follicular phase, the epithelium of the vagina is stratifi ed squamous and cornifi ed. Females at early, middle and term pregnancy have a columnar stratifi ed epithelium with mucous cells. Glycoproteins in the mucous cells were detected using PAS, PA ∗ S, KOH/PA ∗ /BH/PAS; and Alcian blue, pH 0.5, pH 1, pH 2.5 and 0.006 M). Lectin histochemistry showed that UEA-I and RCA-1 lectins reacted strongly or moderately with epithelial cells in all stages analyzed. These results indicate the presence of L-fucose and â -galactose. Binding with other lectins was variable.
publishDate 2012
dc.date.none.fl_str_mv 2012-05
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/272993
Flamini, Mirta Alicia; Díaz, Alcira Ofelia; Barbeito, Claudio Gustavo; Portiansky, Enrique Leo; Morphology, morphometry, histochemistry and lectin histochemistry of the vagina of the plains viscacha (Lagostomus maximus); Informa Healthcare; Biotechnic and Histochemistry; 87; 2; 5-2012; 81-94
1052-0295
CONICET Digital
CONICET
url http://hdl.handle.net/11336/272993
identifier_str_mv Flamini, Mirta Alicia; Díaz, Alcira Ofelia; Barbeito, Claudio Gustavo; Portiansky, Enrique Leo; Morphology, morphometry, histochemistry and lectin histochemistry of the vagina of the plains viscacha (Lagostomus maximus); Informa Healthcare; Biotechnic and Histochemistry; 87; 2; 5-2012; 81-94
1052-0295
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.tandfonline.com/doi/full/10.3109/10520295.2010.518497
info:eu-repo/semantics/altIdentifier/doi/10.3109/10520295.2010.518497
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Informa Healthcare
publisher.none.fl_str_mv Informa Healthcare
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.22299

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