Glycoproteins histochemistry of the gills of Odontesthes bonariensis (Teleostei, Atherinopsidae)

Autores
Díaz, Alcira Ofelia; García, A.M.; Escalante, Alicia Haydee; Goldemberg, A.L.
Año de publicación
2010
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The histochemistry of glycoproteins (GP) in the mucous cells of the gills of the silverside Odontesthes bonariensis was identified with: (1) oxidizable vicinal diols; (2) sialic acid and some of their chain variants, carbon 7 (7C), carbon 8 (8C) or carbon 9 (9C); (3) sialic acid residues without O‐acyl substitution and with O‐acyl substitution at 7C, 8C or 9C; (4) carboxyl groups and (5) sulphate groups. A battery of seven biotinylated lectins allowed GPs sugar residues to be distinguished. Mucous cells showed the presence of neutral, sulphated and sialylated GPs. Dolichos biflorus agglutinin (DBA) and Glycine max agglutinin (SBA) showed strong positive staining; Arachis hypogaea agglutinin (PNA), Ricinus communis agglutinin‐I (RCA‐I) and Triticum vulgaris agglutinin (WGA) showed moderate staining, while Ulex europaeus agglutinin‐I (UEA‐I) was completely negative.
Fil: Díaz, Alcira Ofelia. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Departamento de Biología; Argentina
Fil: García, A.M.. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Escalante, Alicia Haydee. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Departamento de Biología; Argentina
Fil: Goldemberg, A.L.. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Departamento de Biología; Argentina
Materia
HISTOCHEMICAL REACTIONS
MUCOUS CELLS
SILVERSIDE
TELEOSTS
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/96392
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/96392
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Glycoproteins histochemistry of the gills of Odontesthes bonariensis (Teleostei, Atherinopsidae)Díaz, Alcira OfeliaGarcía, A.M.Escalante, Alicia HaydeeGoldemberg, A.L.HISTOCHEMICAL REACTIONSMUCOUS CELLSSILVERSIDETELEOSTShttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1https://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1The histochemistry of glycoproteins (GP) in the mucous cells of the gills of the silverside Odontesthes bonariensis was identified with: (1) oxidizable vicinal diols; (2) sialic acid and some of their chain variants, carbon 7 (7C), carbon 8 (8C) or carbon 9 (9C); (3) sialic acid residues without O‐acyl substitution and with O‐acyl substitution at 7C, 8C or 9C; (4) carboxyl groups and (5) sulphate groups. A battery of seven biotinylated lectins allowed GPs sugar residues to be distinguished. Mucous cells showed the presence of neutral, sulphated and sialylated GPs. Dolichos biflorus agglutinin (DBA) and Glycine max agglutinin (SBA) showed strong positive staining; Arachis hypogaea agglutinin (PNA), Ricinus communis agglutinin‐I (RCA‐I) and Triticum vulgaris agglutinin (WGA) showed moderate staining, while Ulex europaeus agglutinin‐I (UEA‐I) was completely negative.Fil: Díaz, Alcira Ofelia. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Departamento de Biología; ArgentinaFil: García, A.M.. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Escalante, Alicia Haydee. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Departamento de Biología; ArgentinaFil: Goldemberg, A.L.. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Departamento de Biología; ArgentinaWiley Blackwell Publishing, Inc2010-11info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/96392Díaz, Alcira Ofelia; García, A.M.; Escalante, Alicia Haydee; Goldemberg, A.L.; Glycoproteins histochemistry of the gills of Odontesthes bonariensis (Teleostei, Atherinopsidae); Wiley Blackwell Publishing, Inc; Journal of Fish Biology; 77; 7; 11-2010; 1665-16730022-1112CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/abs/10.1111/j.1095-8649.2010.02803.xinfo:eu-repo/semantics/altIdentifier/doi/10.1111/j.1095-8649.2010.02803.xinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T10:01:35Zoai:ri.conicet.gov.ar:11336/96392instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 10:01:35.666CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Glycoproteins histochemistry of the gills of Odontesthes bonariensis (Teleostei, Atherinopsidae)
title Glycoproteins histochemistry of the gills of Odontesthes bonariensis (Teleostei, Atherinopsidae)
spellingShingle Glycoproteins histochemistry of the gills of Odontesthes bonariensis (Teleostei, Atherinopsidae)
Díaz, Alcira Ofelia
HISTOCHEMICAL REACTIONS
MUCOUS CELLS
SILVERSIDE
TELEOSTS
title_short Glycoproteins histochemistry of the gills of Odontesthes bonariensis (Teleostei, Atherinopsidae)
title_full Glycoproteins histochemistry of the gills of Odontesthes bonariensis (Teleostei, Atherinopsidae)
title_fullStr Glycoproteins histochemistry of the gills of Odontesthes bonariensis (Teleostei, Atherinopsidae)
title_full_unstemmed Glycoproteins histochemistry of the gills of Odontesthes bonariensis (Teleostei, Atherinopsidae)
title_sort Glycoproteins histochemistry of the gills of Odontesthes bonariensis (Teleostei, Atherinopsidae)
dc.creator.none.fl_str_mv Díaz, Alcira Ofelia
García, A.M.
Escalante, Alicia Haydee
Goldemberg, A.L.
author Díaz, Alcira Ofelia
author_facet Díaz, Alcira Ofelia
García, A.M.
Escalante, Alicia Haydee
Goldemberg, A.L.
author_role author
author2 García, A.M.
Escalante, Alicia Haydee
Goldemberg, A.L.
author2_role author
author
author
dc.subject.none.fl_str_mv HISTOCHEMICAL REACTIONS
MUCOUS CELLS
SILVERSIDE
TELEOSTS
topic HISTOCHEMICAL REACTIONS
MUCOUS CELLS
SILVERSIDE
TELEOSTS
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv The histochemistry of glycoproteins (GP) in the mucous cells of the gills of the silverside Odontesthes bonariensis was identified with: (1) oxidizable vicinal diols; (2) sialic acid and some of their chain variants, carbon 7 (7C), carbon 8 (8C) or carbon 9 (9C); (3) sialic acid residues without O‐acyl substitution and with O‐acyl substitution at 7C, 8C or 9C; (4) carboxyl groups and (5) sulphate groups. A battery of seven biotinylated lectins allowed GPs sugar residues to be distinguished. Mucous cells showed the presence of neutral, sulphated and sialylated GPs. Dolichos biflorus agglutinin (DBA) and Glycine max agglutinin (SBA) showed strong positive staining; Arachis hypogaea agglutinin (PNA), Ricinus communis agglutinin‐I (RCA‐I) and Triticum vulgaris agglutinin (WGA) showed moderate staining, while Ulex europaeus agglutinin‐I (UEA‐I) was completely negative.
Fil: Díaz, Alcira Ofelia. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Departamento de Biología; Argentina
Fil: García, A.M.. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Escalante, Alicia Haydee. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Departamento de Biología; Argentina
Fil: Goldemberg, A.L.. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Departamento de Biología; Argentina
description The histochemistry of glycoproteins (GP) in the mucous cells of the gills of the silverside Odontesthes bonariensis was identified with: (1) oxidizable vicinal diols; (2) sialic acid and some of their chain variants, carbon 7 (7C), carbon 8 (8C) or carbon 9 (9C); (3) sialic acid residues without O‐acyl substitution and with O‐acyl substitution at 7C, 8C or 9C; (4) carboxyl groups and (5) sulphate groups. A battery of seven biotinylated lectins allowed GPs sugar residues to be distinguished. Mucous cells showed the presence of neutral, sulphated and sialylated GPs. Dolichos biflorus agglutinin (DBA) and Glycine max agglutinin (SBA) showed strong positive staining; Arachis hypogaea agglutinin (PNA), Ricinus communis agglutinin‐I (RCA‐I) and Triticum vulgaris agglutinin (WGA) showed moderate staining, while Ulex europaeus agglutinin‐I (UEA‐I) was completely negative.
publishDate 2010
dc.date.none.fl_str_mv 2010-11
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/96392
Díaz, Alcira Ofelia; García, A.M.; Escalante, Alicia Haydee; Goldemberg, A.L.; Glycoproteins histochemistry of the gills of Odontesthes bonariensis (Teleostei, Atherinopsidae); Wiley Blackwell Publishing, Inc; Journal of Fish Biology; 77; 7; 11-2010; 1665-1673
0022-1112
CONICET Digital
CONICET
url http://hdl.handle.net/11336/96392
identifier_str_mv Díaz, Alcira Ofelia; García, A.M.; Escalante, Alicia Haydee; Goldemberg, A.L.; Glycoproteins histochemistry of the gills of Odontesthes bonariensis (Teleostei, Atherinopsidae); Wiley Blackwell Publishing, Inc; Journal of Fish Biology; 77; 7; 11-2010; 1665-1673
0022-1112
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/abs/10.1111/j.1095-8649.2010.02803.x
info:eu-repo/semantics/altIdentifier/doi/10.1111/j.1095-8649.2010.02803.x
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Wiley Blackwell Publishing, Inc
publisher.none.fl_str_mv Wiley Blackwell Publishing, Inc
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1842269705970122752
score 13.13397

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