Different MK-801 administration schedules induce mild to severe learning impairments in an operant conditioning task: role of buspirone and risperidone in ameliorating these cognit...

Autores
Rapanelli, Maximiliano; Frick, Luciana Romina; Bernardez Vidal, Micaela; Zanutto, Bonifacio Silvano
Año de publicación
2013
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Blockade of N-methyl-d-aspartate receptor (NMDA) by the noncompetitive NMDA receptor (NMDAR) antagonist MK-801 produces behavioral abnormalities and alterations in prefrontal cortex (PFC) functioning. Due to the critical role of the PFC in operant conditioning task learning, we evaluated the effects of acute, repeated postnatal injections of MK-801 (0.1 mg/kg) on learning performance. We injected Long-Evans rats i.p. with MK-801 (0.1 mg/kg) using three different administration schedules: injection 40 min before beginning the task (during) (n = 12); injection twice daily for six consecutive days prior to beginning the experimental procedures (prior) (n = 12); or twice daily subcutaneous injections from postnatal day 7 to 11 (postnatal) (n = 12). Next, we orally administered risperidone (serotonin receptor 2A and dopamine receptor 2 antagonist, 1 mg/kg) or buspirone (serotonin receptor 1A partial agonist, 10 mg/kg) to animals treated with the MK-801 schedule described above. The postnatal and prior administration schedules produced severe learning deficits, whereas injection of MK-801 just before training sessions had only mild effects on acquisition of an operant conditioning. Risperidone was able to reverse the detrimental effect of MK-801 in the animals that were treated with MK-801 during and prior training sessions. In contrast, buspirone was only effective at mitigating the cognitive deficits induced by MK-801 when administered during the training procedures. The data demonstrates that NMDA antagonism disrupts basic mechanisms of learning in a simple PFC-mediated operant conditioning task, and that buspirone and risperidone failed to attenuate the learning deficits when NMDA neurotransmission was blocked in the early stages of the postnatal period.
Fil: Rapanelli, Maximiliano. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental (i); Argentina
Fil: Frick, Luciana Romina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental (i); Argentina
Fil: Bernardez Vidal, Micaela. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental (i); Argentina
Fil: Zanutto, Bonifacio Silvano. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental (i); Argentina
Materia
Buspirone
Nmdar
Perant Conditioning
Serotonin Receptor
Risperidone/Pharmacology
Serotonin Agents/Pharmacology
Rats
Long-Evans
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/2350
Acceder
id CONICETDig_6487de5c50b26104620aa87ddaf495be
oai_identifier_str oai:ri.conicet.gov.ar:11336/2350
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Different MK-801 administration schedules induce mild to severe learning impairments in an operant conditioning task: role of buspirone and risperidone in ameliorating these cognitive deficitsRapanelli, MaximilianoFrick, Luciana RominaBernardez Vidal, MicaelaZanutto, Bonifacio SilvanoBuspironeNmdarPerant ConditioningSerotonin ReceptorRisperidone/PharmacologySerotonin Agents/PharmacologyRatsLong-Evanshttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3https://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3Blockade of N-methyl-d-aspartate receptor (NMDA) by the noncompetitive NMDA receptor (NMDAR) antagonist MK-801 produces behavioral abnormalities and alterations in prefrontal cortex (PFC) functioning. Due to the critical role of the PFC in operant conditioning task learning, we evaluated the effects of acute, repeated postnatal injections of MK-801 (0.1 mg/kg) on learning performance. We injected Long-Evans rats i.p. with MK-801 (0.1 mg/kg) using three different administration schedules: injection 40 min before beginning the task (during) (n = 12); injection twice daily for six consecutive days prior to beginning the experimental procedures (prior) (n = 12); or twice daily subcutaneous injections from postnatal day 7 to 11 (postnatal) (n = 12). Next, we orally administered risperidone (serotonin receptor 2A and dopamine receptor 2 antagonist, 1 mg/kg) or buspirone (serotonin receptor 1A partial agonist, 10 mg/kg) to animals treated with the MK-801 schedule described above. The postnatal and prior administration schedules produced severe learning deficits, whereas injection of MK-801 just before training sessions had only mild effects on acquisition of an operant conditioning. Risperidone was able to reverse the detrimental effect of MK-801 in the animals that were treated with MK-801 during and prior training sessions. In contrast, buspirone was only effective at mitigating the cognitive deficits induced by MK-801 when administered during the training procedures. The data demonstrates that NMDA antagonism disrupts basic mechanisms of learning in a simple PFC-mediated operant conditioning task, and that buspirone and risperidone failed to attenuate the learning deficits when NMDA neurotransmission was blocked in the early stages of the postnatal period.Fil: Rapanelli, Maximiliano. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental (i); ArgentinaFil: Frick, Luciana Romina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental (i); ArgentinaFil: Bernardez Vidal, Micaela. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental (i); ArgentinaFil: Zanutto, Bonifacio Silvano. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental (i); ArgentinaElsevier Science2013-10-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/2350Rapanelli, Maximiliano; Frick, Luciana Romina; Bernardez Vidal, Micaela; Zanutto, Bonifacio Silvano; Different MK-801 administration schedules induce mild to severe learning impairments in an operant conditioning task: role of buspirone and risperidone in ameliorating these cognitive deficits; Elsevier Science; Behavioural Brain Research; 257; 1-10-2013; 156-1650166-4328enginfo:eu-repo/semantics/altIdentifier/doi/doi:10.1016/j.bbr.2013.09.043info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0166432813006013info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:51:20Zoai:ri.conicet.gov.ar:11336/2350instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:51:20.869CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Different MK-801 administration schedules induce mild to severe learning impairments in an operant conditioning task: role of buspirone and risperidone in ameliorating these cognitive deficits
title Different MK-801 administration schedules induce mild to severe learning impairments in an operant conditioning task: role of buspirone and risperidone in ameliorating these cognitive deficits
spellingShingle Different MK-801 administration schedules induce mild to severe learning impairments in an operant conditioning task: role of buspirone and risperidone in ameliorating these cognitive deficits
Rapanelli, Maximiliano
Buspirone
Nmdar
Perant Conditioning
Serotonin Receptor
Risperidone/Pharmacology
Serotonin Agents/Pharmacology
Rats
Long-Evans
title_short Different MK-801 administration schedules induce mild to severe learning impairments in an operant conditioning task: role of buspirone and risperidone in ameliorating these cognitive deficits
title_full Different MK-801 administration schedules induce mild to severe learning impairments in an operant conditioning task: role of buspirone and risperidone in ameliorating these cognitive deficits
title_fullStr Different MK-801 administration schedules induce mild to severe learning impairments in an operant conditioning task: role of buspirone and risperidone in ameliorating these cognitive deficits
title_full_unstemmed Different MK-801 administration schedules induce mild to severe learning impairments in an operant conditioning task: role of buspirone and risperidone in ameliorating these cognitive deficits
title_sort Different MK-801 administration schedules induce mild to severe learning impairments in an operant conditioning task: role of buspirone and risperidone in ameliorating these cognitive deficits
dc.creator.none.fl_str_mv Rapanelli, Maximiliano
Frick, Luciana Romina
Bernardez Vidal, Micaela
Zanutto, Bonifacio Silvano
author Rapanelli, Maximiliano
author_facet Rapanelli, Maximiliano
Frick, Luciana Romina
Bernardez Vidal, Micaela
Zanutto, Bonifacio Silvano
author_role author
author2 Frick, Luciana Romina
Bernardez Vidal, Micaela
Zanutto, Bonifacio Silvano
author2_role author
author
author
dc.subject.none.fl_str_mv Buspirone
Nmdar
Perant Conditioning
Serotonin Receptor
Risperidone/Pharmacology
Serotonin Agents/Pharmacology
Rats
Long-Evans
topic Buspirone
Nmdar
Perant Conditioning
Serotonin Receptor
Risperidone/Pharmacology
Serotonin Agents/Pharmacology
Rats
Long-Evans
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Blockade of N-methyl-d-aspartate receptor (NMDA) by the noncompetitive NMDA receptor (NMDAR) antagonist MK-801 produces behavioral abnormalities and alterations in prefrontal cortex (PFC) functioning. Due to the critical role of the PFC in operant conditioning task learning, we evaluated the effects of acute, repeated postnatal injections of MK-801 (0.1 mg/kg) on learning performance. We injected Long-Evans rats i.p. with MK-801 (0.1 mg/kg) using three different administration schedules: injection 40 min before beginning the task (during) (n = 12); injection twice daily for six consecutive days prior to beginning the experimental procedures (prior) (n = 12); or twice daily subcutaneous injections from postnatal day 7 to 11 (postnatal) (n = 12). Next, we orally administered risperidone (serotonin receptor 2A and dopamine receptor 2 antagonist, 1 mg/kg) or buspirone (serotonin receptor 1A partial agonist, 10 mg/kg) to animals treated with the MK-801 schedule described above. The postnatal and prior administration schedules produced severe learning deficits, whereas injection of MK-801 just before training sessions had only mild effects on acquisition of an operant conditioning. Risperidone was able to reverse the detrimental effect of MK-801 in the animals that were treated with MK-801 during and prior training sessions. In contrast, buspirone was only effective at mitigating the cognitive deficits induced by MK-801 when administered during the training procedures. The data demonstrates that NMDA antagonism disrupts basic mechanisms of learning in a simple PFC-mediated operant conditioning task, and that buspirone and risperidone failed to attenuate the learning deficits when NMDA neurotransmission was blocked in the early stages of the postnatal period.
Fil: Rapanelli, Maximiliano. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental (i); Argentina
Fil: Frick, Luciana Romina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental (i); Argentina
Fil: Bernardez Vidal, Micaela. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental (i); Argentina
Fil: Zanutto, Bonifacio Silvano. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental (i); Argentina
description Blockade of N-methyl-d-aspartate receptor (NMDA) by the noncompetitive NMDA receptor (NMDAR) antagonist MK-801 produces behavioral abnormalities and alterations in prefrontal cortex (PFC) functioning. Due to the critical role of the PFC in operant conditioning task learning, we evaluated the effects of acute, repeated postnatal injections of MK-801 (0.1 mg/kg) on learning performance. We injected Long-Evans rats i.p. with MK-801 (0.1 mg/kg) using three different administration schedules: injection 40 min before beginning the task (during) (n = 12); injection twice daily for six consecutive days prior to beginning the experimental procedures (prior) (n = 12); or twice daily subcutaneous injections from postnatal day 7 to 11 (postnatal) (n = 12). Next, we orally administered risperidone (serotonin receptor 2A and dopamine receptor 2 antagonist, 1 mg/kg) or buspirone (serotonin receptor 1A partial agonist, 10 mg/kg) to animals treated with the MK-801 schedule described above. The postnatal and prior administration schedules produced severe learning deficits, whereas injection of MK-801 just before training sessions had only mild effects on acquisition of an operant conditioning. Risperidone was able to reverse the detrimental effect of MK-801 in the animals that were treated with MK-801 during and prior training sessions. In contrast, buspirone was only effective at mitigating the cognitive deficits induced by MK-801 when administered during the training procedures. The data demonstrates that NMDA antagonism disrupts basic mechanisms of learning in a simple PFC-mediated operant conditioning task, and that buspirone and risperidone failed to attenuate the learning deficits when NMDA neurotransmission was blocked in the early stages of the postnatal period.
publishDate 2013
dc.date.none.fl_str_mv 2013-10-01
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/2350
Rapanelli, Maximiliano; Frick, Luciana Romina; Bernardez Vidal, Micaela; Zanutto, Bonifacio Silvano; Different MK-801 administration schedules induce mild to severe learning impairments in an operant conditioning task: role of buspirone and risperidone in ameliorating these cognitive deficits; Elsevier Science; Behavioural Brain Research; 257; 1-10-2013; 156-165
0166-4328
url http://hdl.handle.net/11336/2350
identifier_str_mv Rapanelli, Maximiliano; Frick, Luciana Romina; Bernardez Vidal, Micaela; Zanutto, Bonifacio Silvano; Different MK-801 administration schedules induce mild to severe learning impairments in an operant conditioning task: role of buspirone and risperidone in ameliorating these cognitive deficits; Elsevier Science; Behavioural Brain Research; 257; 1-10-2013; 156-165
0166-4328
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/doi:10.1016/j.bbr.2013.09.043
info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0166432813006013
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Science
publisher.none.fl_str_mv Elsevier Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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