Synthesis and modification of supports with an Alkylamine and their use in Albumin adsorption

Autores
Gomez, Cesar Gerardo; Strumia, Miriam Cristina
Año de publicación
2008
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The morphological effect of polymeric networks (R) modified with terminal amino groups was studied on the adsorption of bovine serum albumin (BSA). Networks of ethylene glycol dimethacrylate and 2-hydroxyethyl methacrylate [poly (EGDMA-co-HEMA)] were synthesized by suspension polymerization, using different EGDMA contents and agitation speeds. These matrices were characterized by FTIR, mercury intrusion porosimetry, SEM, and swelling degree. The increase of the EGDMA concentration led to the formation of networks with the highest crosslinking degree and porosity. An earlier phase separation yielded a higher aggregation of rigid microspheres, also forming stable pore systems. The increase in coalescence frequency, together with the impeller speed, and the decrease of the stabilizer molecules led to an increment in drop size. Large fused aggregates of microspheres were formed with additional loss of small pores as the stirring was increased, attaining also a higher pore volume (Vp) and a slight decrease of the surface area. Once characterized, networks were activated with butanediolglycidyl ether (BDGE), and then reacted with hexamethylenediamine (HMDA) through coupling reaction. Only the RBDGE- HMDA networks synthesized with the highest EGDMA content and agitation speed showed BSA adsorption. Their base matrices exhibited a Vp higher than 1.4 mL/g, which allows easier protein diffusion into the support.
Fil: Gomez, Cesar Gerardo. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Departamento de Química Orgánica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Strumia, Miriam Cristina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto Multidisciplinario de Biología Vegetal. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Instituto Multidisciplinario de Biología Vegetal; Argentina. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Departamento de Química Orgánica; Argentina
Materia
Albumin
Adsorption
Heterogeneous Networks
Macroporous Polymers
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/27060
Acceder
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network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Synthesis and modification of supports with an Alkylamine and their use in Albumin adsorptionGomez, Cesar GerardoStrumia, Miriam CristinaAlbuminAdsorptionHeterogeneous NetworksMacroporous Polymershttps://purl.org/becyt/ford/1.4https://purl.org/becyt/ford/1The morphological effect of polymeric networks (R) modified with terminal amino groups was studied on the adsorption of bovine serum albumin (BSA). Networks of ethylene glycol dimethacrylate and 2-hydroxyethyl methacrylate [poly (EGDMA-co-HEMA)] were synthesized by suspension polymerization, using different EGDMA contents and agitation speeds. These matrices were characterized by FTIR, mercury intrusion porosimetry, SEM, and swelling degree. The increase of the EGDMA concentration led to the formation of networks with the highest crosslinking degree and porosity. An earlier phase separation yielded a higher aggregation of rigid microspheres, also forming stable pore systems. The increase in coalescence frequency, together with the impeller speed, and the decrease of the stabilizer molecules led to an increment in drop size. Large fused aggregates of microspheres were formed with additional loss of small pores as the stirring was increased, attaining also a higher pore volume (Vp) and a slight decrease of the surface area. Once characterized, networks were activated with butanediolglycidyl ether (BDGE), and then reacted with hexamethylenediamine (HMDA) through coupling reaction. Only the RBDGE- HMDA networks synthesized with the highest EGDMA content and agitation speed showed BSA adsorption. Their base matrices exhibited a Vp higher than 1.4 mL/g, which allows easier protein diffusion into the support.Fil: Gomez, Cesar Gerardo. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Departamento de Química Orgánica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Strumia, Miriam Cristina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto Multidisciplinario de Biología Vegetal. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Instituto Multidisciplinario de Biología Vegetal; Argentina. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Departamento de Química Orgánica; ArgentinaWiley2008-02info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/27060Gomez, Cesar Gerardo; Strumia, Miriam Cristina; Synthesis and modification of supports with an Alkylamine and their use in Albumin adsorption; Wiley; Journal of Polymer Science Part A: Polymer Chemistry; 46; 7; 2-2008; 2557-25660887-624X1099-0518CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1002/pola.22587info:eu-repo/semantics/altIdentifier/url/http://onlinelibrary.wiley.com/doi/10.1002/pola.22587/abstractinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:53:49Zoai:ri.conicet.gov.ar:11336/27060instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:53:49.976CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Synthesis and modification of supports with an Alkylamine and their use in Albumin adsorption
title Synthesis and modification of supports with an Alkylamine and their use in Albumin adsorption
spellingShingle Synthesis and modification of supports with an Alkylamine and their use in Albumin adsorption
Gomez, Cesar Gerardo
Albumin
Adsorption
Heterogeneous Networks
Macroporous Polymers
title_short Synthesis and modification of supports with an Alkylamine and their use in Albumin adsorption
title_full Synthesis and modification of supports with an Alkylamine and their use in Albumin adsorption
title_fullStr Synthesis and modification of supports with an Alkylamine and their use in Albumin adsorption
title_full_unstemmed Synthesis and modification of supports with an Alkylamine and their use in Albumin adsorption
title_sort Synthesis and modification of supports with an Alkylamine and their use in Albumin adsorption
dc.creator.none.fl_str_mv Gomez, Cesar Gerardo
Strumia, Miriam Cristina
author Gomez, Cesar Gerardo
author_facet Gomez, Cesar Gerardo
Strumia, Miriam Cristina
author_role author
author2 Strumia, Miriam Cristina
author2_role author
dc.subject.none.fl_str_mv Albumin
Adsorption
Heterogeneous Networks
Macroporous Polymers
topic Albumin
Adsorption
Heterogeneous Networks
Macroporous Polymers
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.4
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv The morphological effect of polymeric networks (R) modified with terminal amino groups was studied on the adsorption of bovine serum albumin (BSA). Networks of ethylene glycol dimethacrylate and 2-hydroxyethyl methacrylate [poly (EGDMA-co-HEMA)] were synthesized by suspension polymerization, using different EGDMA contents and agitation speeds. These matrices were characterized by FTIR, mercury intrusion porosimetry, SEM, and swelling degree. The increase of the EGDMA concentration led to the formation of networks with the highest crosslinking degree and porosity. An earlier phase separation yielded a higher aggregation of rigid microspheres, also forming stable pore systems. The increase in coalescence frequency, together with the impeller speed, and the decrease of the stabilizer molecules led to an increment in drop size. Large fused aggregates of microspheres were formed with additional loss of small pores as the stirring was increased, attaining also a higher pore volume (Vp) and a slight decrease of the surface area. Once characterized, networks were activated with butanediolglycidyl ether (BDGE), and then reacted with hexamethylenediamine (HMDA) through coupling reaction. Only the RBDGE- HMDA networks synthesized with the highest EGDMA content and agitation speed showed BSA adsorption. Their base matrices exhibited a Vp higher than 1.4 mL/g, which allows easier protein diffusion into the support.
Fil: Gomez, Cesar Gerardo. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Departamento de Química Orgánica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Strumia, Miriam Cristina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto Multidisciplinario de Biología Vegetal. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Instituto Multidisciplinario de Biología Vegetal; Argentina. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Departamento de Química Orgánica; Argentina
description The morphological effect of polymeric networks (R) modified with terminal amino groups was studied on the adsorption of bovine serum albumin (BSA). Networks of ethylene glycol dimethacrylate and 2-hydroxyethyl methacrylate [poly (EGDMA-co-HEMA)] were synthesized by suspension polymerization, using different EGDMA contents and agitation speeds. These matrices were characterized by FTIR, mercury intrusion porosimetry, SEM, and swelling degree. The increase of the EGDMA concentration led to the formation of networks with the highest crosslinking degree and porosity. An earlier phase separation yielded a higher aggregation of rigid microspheres, also forming stable pore systems. The increase in coalescence frequency, together with the impeller speed, and the decrease of the stabilizer molecules led to an increment in drop size. Large fused aggregates of microspheres were formed with additional loss of small pores as the stirring was increased, attaining also a higher pore volume (Vp) and a slight decrease of the surface area. Once characterized, networks were activated with butanediolglycidyl ether (BDGE), and then reacted with hexamethylenediamine (HMDA) through coupling reaction. Only the RBDGE- HMDA networks synthesized with the highest EGDMA content and agitation speed showed BSA adsorption. Their base matrices exhibited a Vp higher than 1.4 mL/g, which allows easier protein diffusion into the support.
publishDate 2008
dc.date.none.fl_str_mv 2008-02
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/27060
Gomez, Cesar Gerardo; Strumia, Miriam Cristina; Synthesis and modification of supports with an Alkylamine and their use in Albumin adsorption; Wiley; Journal of Polymer Science Part A: Polymer Chemistry; 46; 7; 2-2008; 2557-2566
0887-624X
1099-0518
CONICET Digital
CONICET
url http://hdl.handle.net/11336/27060
identifier_str_mv Gomez, Cesar Gerardo; Strumia, Miriam Cristina; Synthesis and modification of supports with an Alkylamine and their use in Albumin adsorption; Wiley; Journal of Polymer Science Part A: Polymer Chemistry; 46; 7; 2-2008; 2557-2566
0887-624X
1099-0518
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1002/pola.22587
info:eu-repo/semantics/altIdentifier/url/http://onlinelibrary.wiley.com/doi/10.1002/pola.22587/abstract
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Wiley
publisher.none.fl_str_mv Wiley
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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