Study of Amino ligands fixation to Macroporous supports and their influence on Albumin adsorption

Autores
Gomez, Cesar Gerardo; Strumia, Miriam Cristina
Año de publicación
2009
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Heterogeneous networks of ethylene glycol dimethacrylate and 2-hydroxyethyl methacrylate [poly(EGDMA-co-HEMA)] were synthesized by suspension polymerization using different EGDMA contents and agitation speeds. The networks were actived with epichlorhydrine (Ech) or 1,4-butanediol diglycidyl ether (BDGE), and then hexamethylenediamine (HMDA) or ethylenediamine (EDA) were conjugated to the support by coupling reaction. Here, a higher alkyldiamine concentration and temperature, and a longer reaction time led to higher yields. Amino ligands of the support III were used to analyze their adsorption performance of bovine serum albumin (BSA) from the adsorption kinetic. A more external location of HMDA amino ligands into network led to get the maximum adsorption in a time shorter than that with EDA. Due to its bigger size, the HMDA molecule was attached mostly to the network surface between larger pores, which favored a faster protein adsorption. When derivatives containing BDGE were compared, the EDA ligand displayed a BSA retention higher than that with HMDA, because a shorter separation between the ammonium groups along the spacer arm yielded a stronger electrostatic attraction on the protein. Clearly, the balance obtained between the pores system and the reagents molecular structure used in the formation of Ech-HMDA generated the most efficient BSA adsorption.
Fil: Gomez, Cesar Gerardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto Multidisciplinario de Biología Vegetal. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Instituto Multidisciplinario de Biología Vegetal; Argentina
Fil: Strumia, Miriam Cristina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto Multidisciplinario de Biología Vegetal. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Instituto Multidisciplinario de Biología Vegetal; Argentina
Materia
Albumin Adsorption
Hema
Heterogeneous Polymers
Ion Exchanger
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/24290
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/24290
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Study of Amino ligands fixation to Macroporous supports and their influence on Albumin adsorptionGomez, Cesar GerardoStrumia, Miriam CristinaAlbumin AdsorptionHemaHeterogeneous PolymersIon Exchangerhttps://purl.org/becyt/ford/1.4https://purl.org/becyt/ford/1Heterogeneous networks of ethylene glycol dimethacrylate and 2-hydroxyethyl methacrylate [poly(EGDMA-co-HEMA)] were synthesized by suspension polymerization using different EGDMA contents and agitation speeds. The networks were actived with epichlorhydrine (Ech) or 1,4-butanediol diglycidyl ether (BDGE), and then hexamethylenediamine (HMDA) or ethylenediamine (EDA) were conjugated to the support by coupling reaction. Here, a higher alkyldiamine concentration and temperature, and a longer reaction time led to higher yields. Amino ligands of the support III were used to analyze their adsorption performance of bovine serum albumin (BSA) from the adsorption kinetic. A more external location of HMDA amino ligands into network led to get the maximum adsorption in a time shorter than that with EDA. Due to its bigger size, the HMDA molecule was attached mostly to the network surface between larger pores, which favored a faster protein adsorption. When derivatives containing BDGE were compared, the EDA ligand displayed a BSA retention higher than that with HMDA, because a shorter separation between the ammonium groups along the spacer arm yielded a stronger electrostatic attraction on the protein. Clearly, the balance obtained between the pores system and the reagents molecular structure used in the formation of Ech-HMDA generated the most efficient BSA adsorption.Fil: Gomez, Cesar Gerardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto Multidisciplinario de Biología Vegetal. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Instituto Multidisciplinario de Biología Vegetal; ArgentinaFil: Strumia, Miriam Cristina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto Multidisciplinario de Biología Vegetal. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Instituto Multidisciplinario de Biología Vegetal; ArgentinaWiley2009-11info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/24290Gomez, Cesar Gerardo; Strumia, Miriam Cristina; Study of Amino ligands fixation to Macroporous supports and their influence on Albumin adsorption; Wiley; Journal of Polymer Science Part A: Polymer Chemistry; 47; 24; 11-2009; 6771-67820887-624X1099-0518CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1002/pola.23717info:eu-repo/semantics/altIdentifier/url/http://onlinelibrary.wiley.com/doi/10.1002/pola.23717/abstractinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:31:43Zoai:ri.conicet.gov.ar:11336/24290instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:31:43.841CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Study of Amino ligands fixation to Macroporous supports and their influence on Albumin adsorption
title Study of Amino ligands fixation to Macroporous supports and their influence on Albumin adsorption
spellingShingle Study of Amino ligands fixation to Macroporous supports and their influence on Albumin adsorption
Gomez, Cesar Gerardo
Albumin Adsorption
Hema
Heterogeneous Polymers
Ion Exchanger
title_short Study of Amino ligands fixation to Macroporous supports and their influence on Albumin adsorption
title_full Study of Amino ligands fixation to Macroporous supports and their influence on Albumin adsorption
title_fullStr Study of Amino ligands fixation to Macroporous supports and their influence on Albumin adsorption
title_full_unstemmed Study of Amino ligands fixation to Macroporous supports and their influence on Albumin adsorption
title_sort Study of Amino ligands fixation to Macroporous supports and their influence on Albumin adsorption
dc.creator.none.fl_str_mv Gomez, Cesar Gerardo
Strumia, Miriam Cristina
author Gomez, Cesar Gerardo
author_facet Gomez, Cesar Gerardo
Strumia, Miriam Cristina
author_role author
author2 Strumia, Miriam Cristina
author2_role author
dc.subject.none.fl_str_mv Albumin Adsorption
Hema
Heterogeneous Polymers
Ion Exchanger
topic Albumin Adsorption
Hema
Heterogeneous Polymers
Ion Exchanger
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.4
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Heterogeneous networks of ethylene glycol dimethacrylate and 2-hydroxyethyl methacrylate [poly(EGDMA-co-HEMA)] were synthesized by suspension polymerization using different EGDMA contents and agitation speeds. The networks were actived with epichlorhydrine (Ech) or 1,4-butanediol diglycidyl ether (BDGE), and then hexamethylenediamine (HMDA) or ethylenediamine (EDA) were conjugated to the support by coupling reaction. Here, a higher alkyldiamine concentration and temperature, and a longer reaction time led to higher yields. Amino ligands of the support III were used to analyze their adsorption performance of bovine serum albumin (BSA) from the adsorption kinetic. A more external location of HMDA amino ligands into network led to get the maximum adsorption in a time shorter than that with EDA. Due to its bigger size, the HMDA molecule was attached mostly to the network surface between larger pores, which favored a faster protein adsorption. When derivatives containing BDGE were compared, the EDA ligand displayed a BSA retention higher than that with HMDA, because a shorter separation between the ammonium groups along the spacer arm yielded a stronger electrostatic attraction on the protein. Clearly, the balance obtained between the pores system and the reagents molecular structure used in the formation of Ech-HMDA generated the most efficient BSA adsorption.
Fil: Gomez, Cesar Gerardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto Multidisciplinario de Biología Vegetal. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Instituto Multidisciplinario de Biología Vegetal; Argentina
Fil: Strumia, Miriam Cristina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto Multidisciplinario de Biología Vegetal. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Instituto Multidisciplinario de Biología Vegetal; Argentina
description Heterogeneous networks of ethylene glycol dimethacrylate and 2-hydroxyethyl methacrylate [poly(EGDMA-co-HEMA)] were synthesized by suspension polymerization using different EGDMA contents and agitation speeds. The networks were actived with epichlorhydrine (Ech) or 1,4-butanediol diglycidyl ether (BDGE), and then hexamethylenediamine (HMDA) or ethylenediamine (EDA) were conjugated to the support by coupling reaction. Here, a higher alkyldiamine concentration and temperature, and a longer reaction time led to higher yields. Amino ligands of the support III were used to analyze their adsorption performance of bovine serum albumin (BSA) from the adsorption kinetic. A more external location of HMDA amino ligands into network led to get the maximum adsorption in a time shorter than that with EDA. Due to its bigger size, the HMDA molecule was attached mostly to the network surface between larger pores, which favored a faster protein adsorption. When derivatives containing BDGE were compared, the EDA ligand displayed a BSA retention higher than that with HMDA, because a shorter separation between the ammonium groups along the spacer arm yielded a stronger electrostatic attraction on the protein. Clearly, the balance obtained between the pores system and the reagents molecular structure used in the formation of Ech-HMDA generated the most efficient BSA adsorption.
publishDate 2009
dc.date.none.fl_str_mv 2009-11
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/24290
Gomez, Cesar Gerardo; Strumia, Miriam Cristina; Study of Amino ligands fixation to Macroporous supports and their influence on Albumin adsorption; Wiley; Journal of Polymer Science Part A: Polymer Chemistry; 47; 24; 11-2009; 6771-6782
0887-624X
1099-0518
CONICET Digital
CONICET
url http://hdl.handle.net/11336/24290
identifier_str_mv Gomez, Cesar Gerardo; Strumia, Miriam Cristina; Study of Amino ligands fixation to Macroporous supports and their influence on Albumin adsorption; Wiley; Journal of Polymer Science Part A: Polymer Chemistry; 47; 24; 11-2009; 6771-6782
0887-624X
1099-0518
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1002/pola.23717
info:eu-repo/semantics/altIdentifier/url/http://onlinelibrary.wiley.com/doi/10.1002/pola.23717/abstract
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Wiley
publisher.none.fl_str_mv Wiley
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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