Endothelial Lipase Activity Predicts High-Density Lipoprotein Catabolism in Hemodialysis

Autores
Miksztowicz, Veronica Julieta; McCoy, Mary G.; Schreier, Laura Ester; Cacciagiú, Leonardo Damian; Elbert, Alicia; González, Ana Inés; Billheimer, Jeffrey; Eacho, Patrick; Rader, Daniel J.; Berg, Gabriela Alicia
Año de publicación
2012
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Objective: A novel phospholipase assay was used to measure for the first time the behavior of endothelial and hepatic phospholipase activities in postheparin human plasma of hemodialyzed patients and its relationship with atherogenic and antiatherogenic lipoprotein levels.Methods and Results:Endothelial and hepatic phospholipase activity was assessed in a total SN1-specific phospholipase assay, using (1-decanoylthio-1-deoxy-2-decanoyl-sn-glycero-3-phosphoryl) ethylene glycol as the substrate. Hemodialyzed patients presented lower values of total and hepatic phospholipase activity than controls: 4.4 (1.9?9.0) versus 7.5 (3.6-18.0) and 2.6 (0.7-6.2) versus 6.6 (1.3-15.2) μmol of fatty acid released per milliliter of postheparin plasma per hour, respectively (P<0.001); however, endothelial lipase (EL) phospholipase activity was increased in patients: 1.7 (0.8-3.0) versus 1.1 (0.1-2.7) μmol of fatty acid released per milliliter of postheparin plasma per hour (P=0.008). EL was negatively associated with high-density lipoprotein (HDL)-cholesterol (r=-0.427; P=0.001), and apolipoprotein A-I levels, total phospholipase, and hepatic lipase activity were directly associated with low-density lipoprotein-cholesterol and apolipoprotein B. The association of EL and HDL-cholesterol remained significant when adjusting for waist circumference (β=-0.26; P=0.05), and the effect of hepatic lipase on low-density lipoprotein-cholesterol continued after adjusting for age (β=0.46; P= 0.001).Conclusion: Our results support the hypothesis that EL is the predominant enzyme responsible for lipolytic catabolism of HDLs in hemodialyzed patients and resolve the apparent paradox observed between low hepatic lipase activity and decreased HDL-cholesterol levels observed in these patients. In addition, the ability to assess total hepatic lipase and EL phospholipase activity in plasma will increase our knowledge of the mechanisms involved in controlling HDL levels and cardiovascular risk in hemodialyzed patients, as well as other populations with low levels of HDL-cholesterol.
Fil: Miksztowicz, Veronica Julieta. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Bioquímica Clínica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: McCoy, Mary G.. Institute For Translational Medicine And Therapeutics; Estados Unidos
Fil: Schreier, Laura Ester. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Bioquímica Clínica; Argentina
Fil: Cacciagiú, Leonardo Damian. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Bioquímica Clínica; Argentina
Fil: Elbert, Alicia. No especifíca;
Fil: González, Ana Inés. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Bioquímica Clínica; Argentina
Fil: Billheimer, Jeffrey. Institute For Translational Medicine And Therapeutics; Estados Unidos
Fil: Eacho, Patrick. Lilly Research Laboratories, Eli Lilly And Company; Estados Unidos
Fil: Rader, Daniel J.. Institute For Translational Medicine And Therapeutics; Estados Unidos
Fil: Berg, Gabriela Alicia. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Bioquímica Clínica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Materia
Chronic kidney disease
Endothelial lipase
Hepatic lipase
High-density lipoprotein-cholesterol
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/272669
Acceder
id CONICETDig_637c04f3675ab6470917dd43c6d99af7
oai_identifier_str oai:ri.conicet.gov.ar:11336/272669
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Endothelial Lipase Activity Predicts High-Density Lipoprotein Catabolism in HemodialysisMiksztowicz, Veronica JulietaMcCoy, Mary G.Schreier, Laura EsterCacciagiú, Leonardo DamianElbert, AliciaGonzález, Ana InésBillheimer, JeffreyEacho, PatrickRader, Daniel J.Berg, Gabriela AliciaChronic kidney diseaseEndothelial lipaseHepatic lipaseHigh-density lipoprotein-cholesterolhttps://purl.org/becyt/ford/3.3https://purl.org/becyt/ford/3Objective: A novel phospholipase assay was used to measure for the first time the behavior of endothelial and hepatic phospholipase activities in postheparin human plasma of hemodialyzed patients and its relationship with atherogenic and antiatherogenic lipoprotein levels.Methods and Results:Endothelial and hepatic phospholipase activity was assessed in a total SN1-specific phospholipase assay, using (1-decanoylthio-1-deoxy-2-decanoyl-sn-glycero-3-phosphoryl) ethylene glycol as the substrate. Hemodialyzed patients presented lower values of total and hepatic phospholipase activity than controls: 4.4 (1.9?9.0) versus 7.5 (3.6-18.0) and 2.6 (0.7-6.2) versus 6.6 (1.3-15.2) μmol of fatty acid released per milliliter of postheparin plasma per hour, respectively (P<0.001); however, endothelial lipase (EL) phospholipase activity was increased in patients: 1.7 (0.8-3.0) versus 1.1 (0.1-2.7) μmol of fatty acid released per milliliter of postheparin plasma per hour (P=0.008). EL was negatively associated with high-density lipoprotein (HDL)-cholesterol (r=-0.427; P=0.001), and apolipoprotein A-I levels, total phospholipase, and hepatic lipase activity were directly associated with low-density lipoprotein-cholesterol and apolipoprotein B. The association of EL and HDL-cholesterol remained significant when adjusting for waist circumference (β=-0.26; P=0.05), and the effect of hepatic lipase on low-density lipoprotein-cholesterol continued after adjusting for age (β=0.46; P= 0.001).Conclusion: Our results support the hypothesis that EL is the predominant enzyme responsible for lipolytic catabolism of HDLs in hemodialyzed patients and resolve the apparent paradox observed between low hepatic lipase activity and decreased HDL-cholesterol levels observed in these patients. In addition, the ability to assess total hepatic lipase and EL phospholipase activity in plasma will increase our knowledge of the mechanisms involved in controlling HDL levels and cardiovascular risk in hemodialyzed patients, as well as other populations with low levels of HDL-cholesterol.Fil: Miksztowicz, Veronica Julieta. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Bioquímica Clínica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: McCoy, Mary G.. Institute For Translational Medicine And Therapeutics; Estados UnidosFil: Schreier, Laura Ester. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Bioquímica Clínica; ArgentinaFil: Cacciagiú, Leonardo Damian. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Bioquímica Clínica; ArgentinaFil: Elbert, Alicia. No especifíca;Fil: González, Ana Inés. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Bioquímica Clínica; ArgentinaFil: Billheimer, Jeffrey. Institute For Translational Medicine And Therapeutics; Estados UnidosFil: Eacho, Patrick. Lilly Research Laboratories, Eli Lilly And Company; Estados UnidosFil: Rader, Daniel J.. Institute For Translational Medicine And Therapeutics; Estados UnidosFil: Berg, Gabriela Alicia. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Bioquímica Clínica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaLippincott Williams2012-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/272669Miksztowicz, Veronica Julieta; McCoy, Mary G.; Schreier, Laura Ester; Cacciagiú, Leonardo Damian; Elbert, Alicia; et al.; Endothelial Lipase Activity Predicts High-Density Lipoprotein Catabolism in Hemodialysis; Lippincott Williams; Arteriosclerosis, Thrombosis, and Vascular Biology; 32; 12; 12-2012; 3033-30401079-5642CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.ahajournals.org/doi/10.1161/atvbaha.112.300110info:eu-repo/semantics/altIdentifier/doi/10.1161/ATVBAHA.112.300110info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T15:12:12Zoai:ri.conicet.gov.ar:11336/272669instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 15:12:12.285CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Endothelial Lipase Activity Predicts High-Density Lipoprotein Catabolism in Hemodialysis
title Endothelial Lipase Activity Predicts High-Density Lipoprotein Catabolism in Hemodialysis
spellingShingle Endothelial Lipase Activity Predicts High-Density Lipoprotein Catabolism in Hemodialysis
Miksztowicz, Veronica Julieta
Chronic kidney disease
Endothelial lipase
Hepatic lipase
High-density lipoprotein-cholesterol
title_short Endothelial Lipase Activity Predicts High-Density Lipoprotein Catabolism in Hemodialysis
title_full Endothelial Lipase Activity Predicts High-Density Lipoprotein Catabolism in Hemodialysis
title_fullStr Endothelial Lipase Activity Predicts High-Density Lipoprotein Catabolism in Hemodialysis
title_full_unstemmed Endothelial Lipase Activity Predicts High-Density Lipoprotein Catabolism in Hemodialysis
title_sort Endothelial Lipase Activity Predicts High-Density Lipoprotein Catabolism in Hemodialysis
dc.creator.none.fl_str_mv Miksztowicz, Veronica Julieta
McCoy, Mary G.
Schreier, Laura Ester
Cacciagiú, Leonardo Damian
Elbert, Alicia
González, Ana Inés
Billheimer, Jeffrey
Eacho, Patrick
Rader, Daniel J.
Berg, Gabriela Alicia
author Miksztowicz, Veronica Julieta
author_facet Miksztowicz, Veronica Julieta
McCoy, Mary G.
Schreier, Laura Ester
Cacciagiú, Leonardo Damian
Elbert, Alicia
González, Ana Inés
Billheimer, Jeffrey
Eacho, Patrick
Rader, Daniel J.
Berg, Gabriela Alicia
author_role author
author2 McCoy, Mary G.
Schreier, Laura Ester
Cacciagiú, Leonardo Damian
Elbert, Alicia
González, Ana Inés
Billheimer, Jeffrey
Eacho, Patrick
Rader, Daniel J.
Berg, Gabriela Alicia
author2_role author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Chronic kidney disease
Endothelial lipase
Hepatic lipase
High-density lipoprotein-cholesterol
topic Chronic kidney disease
Endothelial lipase
Hepatic lipase
High-density lipoprotein-cholesterol
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.3
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Objective: A novel phospholipase assay was used to measure for the first time the behavior of endothelial and hepatic phospholipase activities in postheparin human plasma of hemodialyzed patients and its relationship with atherogenic and antiatherogenic lipoprotein levels.Methods and Results:Endothelial and hepatic phospholipase activity was assessed in a total SN1-specific phospholipase assay, using (1-decanoylthio-1-deoxy-2-decanoyl-sn-glycero-3-phosphoryl) ethylene glycol as the substrate. Hemodialyzed patients presented lower values of total and hepatic phospholipase activity than controls: 4.4 (1.9?9.0) versus 7.5 (3.6-18.0) and 2.6 (0.7-6.2) versus 6.6 (1.3-15.2) μmol of fatty acid released per milliliter of postheparin plasma per hour, respectively (P<0.001); however, endothelial lipase (EL) phospholipase activity was increased in patients: 1.7 (0.8-3.0) versus 1.1 (0.1-2.7) μmol of fatty acid released per milliliter of postheparin plasma per hour (P=0.008). EL was negatively associated with high-density lipoprotein (HDL)-cholesterol (r=-0.427; P=0.001), and apolipoprotein A-I levels, total phospholipase, and hepatic lipase activity were directly associated with low-density lipoprotein-cholesterol and apolipoprotein B. The association of EL and HDL-cholesterol remained significant when adjusting for waist circumference (β=-0.26; P=0.05), and the effect of hepatic lipase on low-density lipoprotein-cholesterol continued after adjusting for age (β=0.46; P= 0.001).Conclusion: Our results support the hypothesis that EL is the predominant enzyme responsible for lipolytic catabolism of HDLs in hemodialyzed patients and resolve the apparent paradox observed between low hepatic lipase activity and decreased HDL-cholesterol levels observed in these patients. In addition, the ability to assess total hepatic lipase and EL phospholipase activity in plasma will increase our knowledge of the mechanisms involved in controlling HDL levels and cardiovascular risk in hemodialyzed patients, as well as other populations with low levels of HDL-cholesterol.
Fil: Miksztowicz, Veronica Julieta. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Bioquímica Clínica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: McCoy, Mary G.. Institute For Translational Medicine And Therapeutics; Estados Unidos
Fil: Schreier, Laura Ester. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Bioquímica Clínica; Argentina
Fil: Cacciagiú, Leonardo Damian. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Bioquímica Clínica; Argentina
Fil: Elbert, Alicia. No especifíca;
Fil: González, Ana Inés. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Bioquímica Clínica; Argentina
Fil: Billheimer, Jeffrey. Institute For Translational Medicine And Therapeutics; Estados Unidos
Fil: Eacho, Patrick. Lilly Research Laboratories, Eli Lilly And Company; Estados Unidos
Fil: Rader, Daniel J.. Institute For Translational Medicine And Therapeutics; Estados Unidos
Fil: Berg, Gabriela Alicia. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Bioquímica Clínica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
description Objective: A novel phospholipase assay was used to measure for the first time the behavior of endothelial and hepatic phospholipase activities in postheparin human plasma of hemodialyzed patients and its relationship with atherogenic and antiatherogenic lipoprotein levels.Methods and Results:Endothelial and hepatic phospholipase activity was assessed in a total SN1-specific phospholipase assay, using (1-decanoylthio-1-deoxy-2-decanoyl-sn-glycero-3-phosphoryl) ethylene glycol as the substrate. Hemodialyzed patients presented lower values of total and hepatic phospholipase activity than controls: 4.4 (1.9?9.0) versus 7.5 (3.6-18.0) and 2.6 (0.7-6.2) versus 6.6 (1.3-15.2) μmol of fatty acid released per milliliter of postheparin plasma per hour, respectively (P<0.001); however, endothelial lipase (EL) phospholipase activity was increased in patients: 1.7 (0.8-3.0) versus 1.1 (0.1-2.7) μmol of fatty acid released per milliliter of postheparin plasma per hour (P=0.008). EL was negatively associated with high-density lipoprotein (HDL)-cholesterol (r=-0.427; P=0.001), and apolipoprotein A-I levels, total phospholipase, and hepatic lipase activity were directly associated with low-density lipoprotein-cholesterol and apolipoprotein B. The association of EL and HDL-cholesterol remained significant when adjusting for waist circumference (β=-0.26; P=0.05), and the effect of hepatic lipase on low-density lipoprotein-cholesterol continued after adjusting for age (β=0.46; P= 0.001).Conclusion: Our results support the hypothesis that EL is the predominant enzyme responsible for lipolytic catabolism of HDLs in hemodialyzed patients and resolve the apparent paradox observed between low hepatic lipase activity and decreased HDL-cholesterol levels observed in these patients. In addition, the ability to assess total hepatic lipase and EL phospholipase activity in plasma will increase our knowledge of the mechanisms involved in controlling HDL levels and cardiovascular risk in hemodialyzed patients, as well as other populations with low levels of HDL-cholesterol.
publishDate 2012
dc.date.none.fl_str_mv 2012-12
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/272669
Miksztowicz, Veronica Julieta; McCoy, Mary G.; Schreier, Laura Ester; Cacciagiú, Leonardo Damian; Elbert, Alicia; et al.; Endothelial Lipase Activity Predicts High-Density Lipoprotein Catabolism in Hemodialysis; Lippincott Williams; Arteriosclerosis, Thrombosis, and Vascular Biology; 32; 12; 12-2012; 3033-3040
1079-5642
CONICET Digital
CONICET
url http://hdl.handle.net/11336/272669
identifier_str_mv Miksztowicz, Veronica Julieta; McCoy, Mary G.; Schreier, Laura Ester; Cacciagiú, Leonardo Damian; Elbert, Alicia; et al.; Endothelial Lipase Activity Predicts High-Density Lipoprotein Catabolism in Hemodialysis; Lippincott Williams; Arteriosclerosis, Thrombosis, and Vascular Biology; 32; 12; 12-2012; 3033-3040
1079-5642
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.ahajournals.org/doi/10.1161/atvbaha.112.300110
info:eu-repo/semantics/altIdentifier/doi/10.1161/ATVBAHA.112.300110
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
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dc.publisher.none.fl_str_mv Lippincott Williams
publisher.none.fl_str_mv Lippincott Williams
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.22299

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