Tomato fruit development in the auxin-resistant dgt mutant is induced by pollination but not by auxin treatment

Autores
Mignolli, Francesco; Mariotti, Lorenzo; Lombardi, Lara; Vidoz, María Laura; Ceccarelli, Nello; Picciarelli, Piero
Año de publicación
2012
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
In tomato (Solanum lycopersicum Mill.), auxin is believed to play a pivotal role in controlling fruit-set and early ovary growth. In this paper we investigated the effect of the reduced auxin sensitivity exhibited by the diageotropica (dgt) tomato mutant on ovary growth during early stage of fruit development. Here we show that in hand-pollinated ovaries fruit-set was not affected by the dgt lesion while fruit growth was reduced. This reduction was associated with a smaller cell size of mesocarp cells, with a lower mean C values and with a lower gene expression of the expansin gene LeExp2. When a synthetic auxin (4-CPA, chlorophenoxyacetic acid) was applied to the flowers of wild type plants, parthenocarpic ovary growth was induced. Onthe contrary, auxinapplicationto theflowers of dgtplants failed to induceparthenocarpy. Hand-pollinated ovaries of dgt contained higher levels of IAA compared to wild type and this was not associated with high transcript levels of genes encoding a key regulatory enzyme of IAA biosynthesis (ToFZYs) but with lower expression levels of GH3, a gene involved in the conjugation of IAA to amino acids. The expression of diverse Aux/IAA genes and SAUR (small auxin up-regulated RNA) was also altered in the dgt ovaries. The dgt lesion does not seem to affect specific Aux/IAA genes in terms of transcript occurrence but rather in terms of relative levels of expression. Transcript levels of Aux/IAA genes were up regulated in auxin-treated ovaries of wild-type but not in dgt. Together, our results suggest that dgt ovary cells are not able to sense and/or transduce the external auxin signal, whereas pollinated dgt ovary cells are able to detect the IAA present in fertilized ovules promoting fruit development.
Fil: Mignolli, Francesco. Universita Degli Studi Di Pisa; Italia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Nordeste. Instituto de Botánica del Nordeste (i); Argentina
Fil: Mariotti, Lorenzo. Universita Degli Studi Di Pisa; Italia
Fil: Lombardi, Lara. Universita Degli Studi Di Pisa; Italia
Fil: Vidoz, María Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Nordeste. Instituto de Botánica del Nordeste (i); Argentina
Fil: Ceccarelli, Nello . Universita Degli Studi Di Pisa; Italia
Fil: Picciarelli, Piero. Universita Degli Studi Di Pisa; Italia
Materia
Auxin Signalling
Diageotropica Mutant
Fruit Development
Indole-3 Acetic-Acid
Tomato
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/6170
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/6170
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Tomato fruit development in the auxin-resistant dgt mutant is induced by pollination but not by auxin treatmentMignolli, FrancescoMariotti, LorenzoLombardi, LaraVidoz, María LauraCeccarelli, Nello Picciarelli, PieroAuxin SignallingDiageotropica MutantFruit DevelopmentIndole-3 Acetic-AcidTomatohttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1In tomato (Solanum lycopersicum Mill.), auxin is believed to play a pivotal role in controlling fruit-set and early ovary growth. In this paper we investigated the effect of the reduced auxin sensitivity exhibited by the diageotropica (dgt) tomato mutant on ovary growth during early stage of fruit development. Here we show that in hand-pollinated ovaries fruit-set was not affected by the dgt lesion while fruit growth was reduced. This reduction was associated with a smaller cell size of mesocarp cells, with a lower mean C values and with a lower gene expression of the expansin gene LeExp2. When a synthetic auxin (4-CPA, chlorophenoxyacetic acid) was applied to the flowers of wild type plants, parthenocarpic ovary growth was induced. Onthe contrary, auxinapplicationto theflowers of dgtplants failed to induceparthenocarpy. Hand-pollinated ovaries of dgt contained higher levels of IAA compared to wild type and this was not associated with high transcript levels of genes encoding a key regulatory enzyme of IAA biosynthesis (ToFZYs) but with lower expression levels of GH3, a gene involved in the conjugation of IAA to amino acids. The expression of diverse Aux/IAA genes and SAUR (small auxin up-regulated RNA) was also altered in the dgt ovaries. The dgt lesion does not seem to affect specific Aux/IAA genes in terms of transcript occurrence but rather in terms of relative levels of expression. Transcript levels of Aux/IAA genes were up regulated in auxin-treated ovaries of wild-type but not in dgt. Together, our results suggest that dgt ovary cells are not able to sense and/or transduce the external auxin signal, whereas pollinated dgt ovary cells are able to detect the IAA present in fertilized ovules promoting fruit development.Fil: Mignolli, Francesco. Universita Degli Studi Di Pisa; Italia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Nordeste. Instituto de Botánica del Nordeste (i); ArgentinaFil: Mariotti, Lorenzo. Universita Degli Studi Di Pisa; ItaliaFil: Lombardi, Lara. Universita Degli Studi Di Pisa; ItaliaFil: Vidoz, María Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Nordeste. Instituto de Botánica del Nordeste (i); ArgentinaFil: Ceccarelli, Nello . Universita Degli Studi Di Pisa; ItaliaFil: Picciarelli, Piero. Universita Degli Studi Di Pisa; ItaliaElsevier2012-05info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/6170Mignolli, Francesco; Mariotti, Lorenzo; Lombardi, Lara; Vidoz, María Laura; Ceccarelli, Nello ; et al.; Tomato fruit development in the auxin-resistant dgt mutant is induced by pollination but not by auxin treatment; Elsevier; Journal of Plant Physiology; 169; 12; 5-2012; 1165-11720176-1617enginfo:eu-repo/semantics/altIdentifier/doi/info:eu-repo/semantics/altIdentifier/doi/10.1016/j.jplph.2012.04.005info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0176161712001770info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T14:35:57Zoai:ri.conicet.gov.ar:11336/6170instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 14:35:57.66CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Tomato fruit development in the auxin-resistant dgt mutant is induced by pollination but not by auxin treatment
title Tomato fruit development in the auxin-resistant dgt mutant is induced by pollination but not by auxin treatment
spellingShingle Tomato fruit development in the auxin-resistant dgt mutant is induced by pollination but not by auxin treatment
Mignolli, Francesco
Auxin Signalling
Diageotropica Mutant
Fruit Development
Indole-3 Acetic-Acid
Tomato
title_short Tomato fruit development in the auxin-resistant dgt mutant is induced by pollination but not by auxin treatment
title_full Tomato fruit development in the auxin-resistant dgt mutant is induced by pollination but not by auxin treatment
title_fullStr Tomato fruit development in the auxin-resistant dgt mutant is induced by pollination but not by auxin treatment
title_full_unstemmed Tomato fruit development in the auxin-resistant dgt mutant is induced by pollination but not by auxin treatment
title_sort Tomato fruit development in the auxin-resistant dgt mutant is induced by pollination but not by auxin treatment
dc.creator.none.fl_str_mv Mignolli, Francesco
Mariotti, Lorenzo
Lombardi, Lara
Vidoz, María Laura
Ceccarelli, Nello
Picciarelli, Piero
author Mignolli, Francesco
author_facet Mignolli, Francesco
Mariotti, Lorenzo
Lombardi, Lara
Vidoz, María Laura
Ceccarelli, Nello
Picciarelli, Piero
author_role author
author2 Mariotti, Lorenzo
Lombardi, Lara
Vidoz, María Laura
Ceccarelli, Nello
Picciarelli, Piero
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Auxin Signalling
Diageotropica Mutant
Fruit Development
Indole-3 Acetic-Acid
Tomato
topic Auxin Signalling
Diageotropica Mutant
Fruit Development
Indole-3 Acetic-Acid
Tomato
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv In tomato (Solanum lycopersicum Mill.), auxin is believed to play a pivotal role in controlling fruit-set and early ovary growth. In this paper we investigated the effect of the reduced auxin sensitivity exhibited by the diageotropica (dgt) tomato mutant on ovary growth during early stage of fruit development. Here we show that in hand-pollinated ovaries fruit-set was not affected by the dgt lesion while fruit growth was reduced. This reduction was associated with a smaller cell size of mesocarp cells, with a lower mean C values and with a lower gene expression of the expansin gene LeExp2. When a synthetic auxin (4-CPA, chlorophenoxyacetic acid) was applied to the flowers of wild type plants, parthenocarpic ovary growth was induced. Onthe contrary, auxinapplicationto theflowers of dgtplants failed to induceparthenocarpy. Hand-pollinated ovaries of dgt contained higher levels of IAA compared to wild type and this was not associated with high transcript levels of genes encoding a key regulatory enzyme of IAA biosynthesis (ToFZYs) but with lower expression levels of GH3, a gene involved in the conjugation of IAA to amino acids. The expression of diverse Aux/IAA genes and SAUR (small auxin up-regulated RNA) was also altered in the dgt ovaries. The dgt lesion does not seem to affect specific Aux/IAA genes in terms of transcript occurrence but rather in terms of relative levels of expression. Transcript levels of Aux/IAA genes were up regulated in auxin-treated ovaries of wild-type but not in dgt. Together, our results suggest that dgt ovary cells are not able to sense and/or transduce the external auxin signal, whereas pollinated dgt ovary cells are able to detect the IAA present in fertilized ovules promoting fruit development.
Fil: Mignolli, Francesco. Universita Degli Studi Di Pisa; Italia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Nordeste. Instituto de Botánica del Nordeste (i); Argentina
Fil: Mariotti, Lorenzo. Universita Degli Studi Di Pisa; Italia
Fil: Lombardi, Lara. Universita Degli Studi Di Pisa; Italia
Fil: Vidoz, María Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Nordeste. Instituto de Botánica del Nordeste (i); Argentina
Fil: Ceccarelli, Nello . Universita Degli Studi Di Pisa; Italia
Fil: Picciarelli, Piero. Universita Degli Studi Di Pisa; Italia
description In tomato (Solanum lycopersicum Mill.), auxin is believed to play a pivotal role in controlling fruit-set and early ovary growth. In this paper we investigated the effect of the reduced auxin sensitivity exhibited by the diageotropica (dgt) tomato mutant on ovary growth during early stage of fruit development. Here we show that in hand-pollinated ovaries fruit-set was not affected by the dgt lesion while fruit growth was reduced. This reduction was associated with a smaller cell size of mesocarp cells, with a lower mean C values and with a lower gene expression of the expansin gene LeExp2. When a synthetic auxin (4-CPA, chlorophenoxyacetic acid) was applied to the flowers of wild type plants, parthenocarpic ovary growth was induced. Onthe contrary, auxinapplicationto theflowers of dgtplants failed to induceparthenocarpy. Hand-pollinated ovaries of dgt contained higher levels of IAA compared to wild type and this was not associated with high transcript levels of genes encoding a key regulatory enzyme of IAA biosynthesis (ToFZYs) but with lower expression levels of GH3, a gene involved in the conjugation of IAA to amino acids. The expression of diverse Aux/IAA genes and SAUR (small auxin up-regulated RNA) was also altered in the dgt ovaries. The dgt lesion does not seem to affect specific Aux/IAA genes in terms of transcript occurrence but rather in terms of relative levels of expression. Transcript levels of Aux/IAA genes were up regulated in auxin-treated ovaries of wild-type but not in dgt. Together, our results suggest that dgt ovary cells are not able to sense and/or transduce the external auxin signal, whereas pollinated dgt ovary cells are able to detect the IAA present in fertilized ovules promoting fruit development.
publishDate 2012
dc.date.none.fl_str_mv 2012-05
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/6170
Mignolli, Francesco; Mariotti, Lorenzo; Lombardi, Lara; Vidoz, María Laura; Ceccarelli, Nello ; et al.; Tomato fruit development in the auxin-resistant dgt mutant is induced by pollination but not by auxin treatment; Elsevier; Journal of Plant Physiology; 169; 12; 5-2012; 1165-1172
0176-1617
url http://hdl.handle.net/11336/6170
identifier_str_mv Mignolli, Francesco; Mariotti, Lorenzo; Lombardi, Lara; Vidoz, María Laura; Ceccarelli, Nello ; et al.; Tomato fruit development in the auxin-resistant dgt mutant is induced by pollination but not by auxin treatment; Elsevier; Journal of Plant Physiology; 169; 12; 5-2012; 1165-1172
0176-1617
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.jplph.2012.04.005
info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0176161712001770
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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