Tomato fruit development in the auxin-resistant dgt mutant is induced by pollination but not by auxin treatment
- Autores
- Mignolli, Francesco; Mariotti, Lorenzo; Lombardi, Lara; Vidoz, María Laura; Ceccarelli, Nello; Picciarelli, Piero
- Año de publicación
- 2012
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- In tomato (Solanum lycopersicum Mill.), auxin is believed to play a pivotal role in controlling fruit-set and early ovary growth. In this paper we investigated the effect of the reduced auxin sensitivity exhibited by the diageotropica (dgt) tomato mutant on ovary growth during early stage of fruit development. Here we show that in hand-pollinated ovaries fruit-set was not affected by the dgt lesion while fruit growth was reduced. This reduction was associated with a smaller cell size of mesocarp cells, with a lower mean C values and with a lower gene expression of the expansin gene LeExp2. When a synthetic auxin (4-CPA, chlorophenoxyacetic acid) was applied to the flowers of wild type plants, parthenocarpic ovary growth was induced. Onthe contrary, auxinapplicationto theflowers of dgtplants failed to induceparthenocarpy. Hand-pollinated ovaries of dgt contained higher levels of IAA compared to wild type and this was not associated with high transcript levels of genes encoding a key regulatory enzyme of IAA biosynthesis (ToFZYs) but with lower expression levels of GH3, a gene involved in the conjugation of IAA to amino acids. The expression of diverse Aux/IAA genes and SAUR (small auxin up-regulated RNA) was also altered in the dgt ovaries. The dgt lesion does not seem to affect specific Aux/IAA genes in terms of transcript occurrence but rather in terms of relative levels of expression. Transcript levels of Aux/IAA genes were up regulated in auxin-treated ovaries of wild-type but not in dgt. Together, our results suggest that dgt ovary cells are not able to sense and/or transduce the external auxin signal, whereas pollinated dgt ovary cells are able to detect the IAA present in fertilized ovules promoting fruit development.
Fil: Mignolli, Francesco. Universita Degli Studi Di Pisa; Italia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Nordeste. Instituto de Botánica del Nordeste (i); Argentina
Fil: Mariotti, Lorenzo. Universita Degli Studi Di Pisa; Italia
Fil: Lombardi, Lara. Universita Degli Studi Di Pisa; Italia
Fil: Vidoz, María Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Nordeste. Instituto de Botánica del Nordeste (i); Argentina
Fil: Ceccarelli, Nello . Universita Degli Studi Di Pisa; Italia
Fil: Picciarelli, Piero. Universita Degli Studi Di Pisa; Italia - Materia
-
Auxin Signalling
Diageotropica Mutant
Fruit Development
Indole-3 Acetic-Acid
Tomato - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
- Repositorio
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/6170
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CONICET Digital (CONICET) |
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Tomato fruit development in the auxin-resistant dgt mutant is induced by pollination but not by auxin treatmentMignolli, FrancescoMariotti, LorenzoLombardi, LaraVidoz, María LauraCeccarelli, Nello Picciarelli, PieroAuxin SignallingDiageotropica MutantFruit DevelopmentIndole-3 Acetic-AcidTomatohttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1In tomato (Solanum lycopersicum Mill.), auxin is believed to play a pivotal role in controlling fruit-set and early ovary growth. In this paper we investigated the effect of the reduced auxin sensitivity exhibited by the diageotropica (dgt) tomato mutant on ovary growth during early stage of fruit development. Here we show that in hand-pollinated ovaries fruit-set was not affected by the dgt lesion while fruit growth was reduced. This reduction was associated with a smaller cell size of mesocarp cells, with a lower mean C values and with a lower gene expression of the expansin gene LeExp2. When a synthetic auxin (4-CPA, chlorophenoxyacetic acid) was applied to the flowers of wild type plants, parthenocarpic ovary growth was induced. Onthe contrary, auxinapplicationto theflowers of dgtplants failed to induceparthenocarpy. Hand-pollinated ovaries of dgt contained higher levels of IAA compared to wild type and this was not associated with high transcript levels of genes encoding a key regulatory enzyme of IAA biosynthesis (ToFZYs) but with lower expression levels of GH3, a gene involved in the conjugation of IAA to amino acids. The expression of diverse Aux/IAA genes and SAUR (small auxin up-regulated RNA) was also altered in the dgt ovaries. The dgt lesion does not seem to affect specific Aux/IAA genes in terms of transcript occurrence but rather in terms of relative levels of expression. Transcript levels of Aux/IAA genes were up regulated in auxin-treated ovaries of wild-type but not in dgt. Together, our results suggest that dgt ovary cells are not able to sense and/or transduce the external auxin signal, whereas pollinated dgt ovary cells are able to detect the IAA present in fertilized ovules promoting fruit development.Fil: Mignolli, Francesco. Universita Degli Studi Di Pisa; Italia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Nordeste. Instituto de Botánica del Nordeste (i); ArgentinaFil: Mariotti, Lorenzo. Universita Degli Studi Di Pisa; ItaliaFil: Lombardi, Lara. Universita Degli Studi Di Pisa; ItaliaFil: Vidoz, María Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Nordeste. Instituto de Botánica del Nordeste (i); ArgentinaFil: Ceccarelli, Nello . Universita Degli Studi Di Pisa; ItaliaFil: Picciarelli, Piero. Universita Degli Studi Di Pisa; ItaliaElsevier2012-05info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/6170Mignolli, Francesco; Mariotti, Lorenzo; Lombardi, Lara; Vidoz, María Laura; Ceccarelli, Nello ; et al.; Tomato fruit development in the auxin-resistant dgt mutant is induced by pollination but not by auxin treatment; Elsevier; Journal of Plant Physiology; 169; 12; 5-2012; 1165-11720176-1617enginfo:eu-repo/semantics/altIdentifier/doi/info:eu-repo/semantics/altIdentifier/doi/10.1016/j.jplph.2012.04.005info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0176161712001770info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:42:51Zoai:ri.conicet.gov.ar:11336/6170instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:42:51.696CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
dc.title.none.fl_str_mv |
Tomato fruit development in the auxin-resistant dgt mutant is induced by pollination but not by auxin treatment |
title |
Tomato fruit development in the auxin-resistant dgt mutant is induced by pollination but not by auxin treatment |
spellingShingle |
Tomato fruit development in the auxin-resistant dgt mutant is induced by pollination but not by auxin treatment Mignolli, Francesco Auxin Signalling Diageotropica Mutant Fruit Development Indole-3 Acetic-Acid Tomato |
title_short |
Tomato fruit development in the auxin-resistant dgt mutant is induced by pollination but not by auxin treatment |
title_full |
Tomato fruit development in the auxin-resistant dgt mutant is induced by pollination but not by auxin treatment |
title_fullStr |
Tomato fruit development in the auxin-resistant dgt mutant is induced by pollination but not by auxin treatment |
title_full_unstemmed |
Tomato fruit development in the auxin-resistant dgt mutant is induced by pollination but not by auxin treatment |
title_sort |
Tomato fruit development in the auxin-resistant dgt mutant is induced by pollination but not by auxin treatment |
dc.creator.none.fl_str_mv |
Mignolli, Francesco Mariotti, Lorenzo Lombardi, Lara Vidoz, María Laura Ceccarelli, Nello Picciarelli, Piero |
author |
Mignolli, Francesco |
author_facet |
Mignolli, Francesco Mariotti, Lorenzo Lombardi, Lara Vidoz, María Laura Ceccarelli, Nello Picciarelli, Piero |
author_role |
author |
author2 |
Mariotti, Lorenzo Lombardi, Lara Vidoz, María Laura Ceccarelli, Nello Picciarelli, Piero |
author2_role |
author author author author author |
dc.subject.none.fl_str_mv |
Auxin Signalling Diageotropica Mutant Fruit Development Indole-3 Acetic-Acid Tomato |
topic |
Auxin Signalling Diageotropica Mutant Fruit Development Indole-3 Acetic-Acid Tomato |
purl_subject.fl_str_mv |
https://purl.org/becyt/ford/1.6 https://purl.org/becyt/ford/1 |
dc.description.none.fl_txt_mv |
In tomato (Solanum lycopersicum Mill.), auxin is believed to play a pivotal role in controlling fruit-set and early ovary growth. In this paper we investigated the effect of the reduced auxin sensitivity exhibited by the diageotropica (dgt) tomato mutant on ovary growth during early stage of fruit development. Here we show that in hand-pollinated ovaries fruit-set was not affected by the dgt lesion while fruit growth was reduced. This reduction was associated with a smaller cell size of mesocarp cells, with a lower mean C values and with a lower gene expression of the expansin gene LeExp2. When a synthetic auxin (4-CPA, chlorophenoxyacetic acid) was applied to the flowers of wild type plants, parthenocarpic ovary growth was induced. Onthe contrary, auxinapplicationto theflowers of dgtplants failed to induceparthenocarpy. Hand-pollinated ovaries of dgt contained higher levels of IAA compared to wild type and this was not associated with high transcript levels of genes encoding a key regulatory enzyme of IAA biosynthesis (ToFZYs) but with lower expression levels of GH3, a gene involved in the conjugation of IAA to amino acids. The expression of diverse Aux/IAA genes and SAUR (small auxin up-regulated RNA) was also altered in the dgt ovaries. The dgt lesion does not seem to affect specific Aux/IAA genes in terms of transcript occurrence but rather in terms of relative levels of expression. Transcript levels of Aux/IAA genes were up regulated in auxin-treated ovaries of wild-type but not in dgt. Together, our results suggest that dgt ovary cells are not able to sense and/or transduce the external auxin signal, whereas pollinated dgt ovary cells are able to detect the IAA present in fertilized ovules promoting fruit development. Fil: Mignolli, Francesco. Universita Degli Studi Di Pisa; Italia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Nordeste. Instituto de Botánica del Nordeste (i); Argentina Fil: Mariotti, Lorenzo. Universita Degli Studi Di Pisa; Italia Fil: Lombardi, Lara. Universita Degli Studi Di Pisa; Italia Fil: Vidoz, María Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Nordeste. Instituto de Botánica del Nordeste (i); Argentina Fil: Ceccarelli, Nello . Universita Degli Studi Di Pisa; Italia Fil: Picciarelli, Piero. Universita Degli Studi Di Pisa; Italia |
description |
In tomato (Solanum lycopersicum Mill.), auxin is believed to play a pivotal role in controlling fruit-set and early ovary growth. In this paper we investigated the effect of the reduced auxin sensitivity exhibited by the diageotropica (dgt) tomato mutant on ovary growth during early stage of fruit development. Here we show that in hand-pollinated ovaries fruit-set was not affected by the dgt lesion while fruit growth was reduced. This reduction was associated with a smaller cell size of mesocarp cells, with a lower mean C values and with a lower gene expression of the expansin gene LeExp2. When a synthetic auxin (4-CPA, chlorophenoxyacetic acid) was applied to the flowers of wild type plants, parthenocarpic ovary growth was induced. Onthe contrary, auxinapplicationto theflowers of dgtplants failed to induceparthenocarpy. Hand-pollinated ovaries of dgt contained higher levels of IAA compared to wild type and this was not associated with high transcript levels of genes encoding a key regulatory enzyme of IAA biosynthesis (ToFZYs) but with lower expression levels of GH3, a gene involved in the conjugation of IAA to amino acids. The expression of diverse Aux/IAA genes and SAUR (small auxin up-regulated RNA) was also altered in the dgt ovaries. The dgt lesion does not seem to affect specific Aux/IAA genes in terms of transcript occurrence but rather in terms of relative levels of expression. Transcript levels of Aux/IAA genes were up regulated in auxin-treated ovaries of wild-type but not in dgt. Together, our results suggest that dgt ovary cells are not able to sense and/or transduce the external auxin signal, whereas pollinated dgt ovary cells are able to detect the IAA present in fertilized ovules promoting fruit development. |
publishDate |
2012 |
dc.date.none.fl_str_mv |
2012-05 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/6170 Mignolli, Francesco; Mariotti, Lorenzo; Lombardi, Lara; Vidoz, María Laura; Ceccarelli, Nello ; et al.; Tomato fruit development in the auxin-resistant dgt mutant is induced by pollination but not by auxin treatment; Elsevier; Journal of Plant Physiology; 169; 12; 5-2012; 1165-1172 0176-1617 |
url |
http://hdl.handle.net/11336/6170 |
identifier_str_mv |
Mignolli, Francesco; Mariotti, Lorenzo; Lombardi, Lara; Vidoz, María Laura; Ceccarelli, Nello ; et al.; Tomato fruit development in the auxin-resistant dgt mutant is induced by pollination but not by auxin treatment; Elsevier; Journal of Plant Physiology; 169; 12; 5-2012; 1165-1172 0176-1617 |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/doi/ info:eu-repo/semantics/altIdentifier/doi/10.1016/j.jplph.2012.04.005 info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0176161712001770 |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-nd/2.5/ar/ |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/ |
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application/pdf application/pdf application/pdf application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
Elsevier |
publisher.none.fl_str_mv |
Elsevier |
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reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas |
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Consejo Nacional de Investigaciones Científicas y Técnicas |
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CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
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dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
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