Knocking down expression of the auxin-amidohydrolase IAR3 alters defense responses in Solanaceae family plants

Autores
D'ippólito, Sebastián; Vankova, Radomira; Joosten, Matthieu H. A. J.; Casalongue, Claudia; Fiol, Diego Fernando
Año de publicación
2016
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
In plants, indole-3-acetic acid (IAA) amido hydrolases (AHs) participate in auxin homeostasis by releasing free IAA from IAA-amino acid conjugates. We investigated the role of IAR3, a member of the IAA amido hydrolase family, in the response of Solanaceous plants challenged by biotrophic and hemi-biotrophic pathogens. By means of genome inspection and phylogenic analysis we firstly identified IAA-AH sequences and putative IAR3 orthologs in Nicotiana benthamiana, tomato and potato. We evaluated the involvement of IAR3 genes in defense responses by using virus-induced gene silencing. We observed that N. benthamiana and tomato plants with knocked-down expression of IAR3 genes contained lower levels of free IAA and presented altered responses to pathogen attack, including enhanced basal defenses and higher tolerance to infection in susceptible plants. We showed that IAR3 genes are consistently up-regulated in N. benthamiana and tomato upon inoculation with Phytophthora infestans and Cladosporium fulvum respectively. However, IAR3 expression decreased significantly when hypersensitive response was triggered in transgenic tomato plants coexpressing the Cf-4 resistance gene and the avirulence factor Avr4. Altogether, our results indicate that changes in IAR3 expression lead to alteration in auxin homeostasis that ultimately affects plant defense responses.
Fil: D'ippólito, Sebastián. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Biológicas. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Biológicas; Argentina
Fil: Vankova, Radomira. Academy of Sciences of the Czech Republic; República Checa
Fil: Joosten, Matthieu H. A. J.. Wageningen University; Países Bajos
Fil: Casalongue, Claudia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Biológicas. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Biológicas; Argentina
Fil: Fiol, Diego Fernando. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Biológicas. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Biológicas; Argentina
Materia
Auxin
Biotic Stress
Cladosporium Fulvum
Indole-3-Acetic Acid Amido Hydrolases
Nicotiana Benthamiana
Phytophthora Infestans
Solanum Lycopersicum
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/62632
Acceder
id CONICETDig_114bca16012a2bc9b1974f8a57ec56d1
oai_identifier_str oai:ri.conicet.gov.ar:11336/62632
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Knocking down expression of the auxin-amidohydrolase IAR3 alters defense responses in Solanaceae family plantsD'ippólito, SebastiánVankova, RadomiraJoosten, Matthieu H. A. J.Casalongue, ClaudiaFiol, Diego FernandoAuxinBiotic StressCladosporium FulvumIndole-3-Acetic Acid Amido HydrolasesNicotiana BenthamianaPhytophthora InfestansSolanum Lycopersicumhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1In plants, indole-3-acetic acid (IAA) amido hydrolases (AHs) participate in auxin homeostasis by releasing free IAA from IAA-amino acid conjugates. We investigated the role of IAR3, a member of the IAA amido hydrolase family, in the response of Solanaceous plants challenged by biotrophic and hemi-biotrophic pathogens. By means of genome inspection and phylogenic analysis we firstly identified IAA-AH sequences and putative IAR3 orthologs in Nicotiana benthamiana, tomato and potato. We evaluated the involvement of IAR3 genes in defense responses by using virus-induced gene silencing. We observed that N. benthamiana and tomato plants with knocked-down expression of IAR3 genes contained lower levels of free IAA and presented altered responses to pathogen attack, including enhanced basal defenses and higher tolerance to infection in susceptible plants. We showed that IAR3 genes are consistently up-regulated in N. benthamiana and tomato upon inoculation with Phytophthora infestans and Cladosporium fulvum respectively. However, IAR3 expression decreased significantly when hypersensitive response was triggered in transgenic tomato plants coexpressing the Cf-4 resistance gene and the avirulence factor Avr4. Altogether, our results indicate that changes in IAR3 expression lead to alteration in auxin homeostasis that ultimately affects plant defense responses.Fil: D'ippólito, Sebastián. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Biológicas. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Biológicas; ArgentinaFil: Vankova, Radomira. Academy of Sciences of the Czech Republic; República ChecaFil: Joosten, Matthieu H. A. J.. Wageningen University; Países BajosFil: Casalongue, Claudia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Biológicas. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Biológicas; ArgentinaFil: Fiol, Diego Fernando. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Biológicas. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Biológicas; ArgentinaElsevier Ireland2016-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/62632D'ippólito, Sebastián; Vankova, Radomira; Joosten, Matthieu H. A. J.; Casalongue, Claudia; Fiol, Diego Fernando; Knocking down expression of the auxin-amidohydrolase IAR3 alters defense responses in Solanaceae family plants; Elsevier Ireland; Plant Science; 253; 12-2016; 31-390168-9452CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0168945216304691info:eu-repo/semantics/altIdentifier/doi/10.1016/j.plantsci.2016.09.008info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:51:19Zoai:ri.conicet.gov.ar:11336/62632instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:51:19.393CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Knocking down expression of the auxin-amidohydrolase IAR3 alters defense responses in Solanaceae family plants
title Knocking down expression of the auxin-amidohydrolase IAR3 alters defense responses in Solanaceae family plants
spellingShingle Knocking down expression of the auxin-amidohydrolase IAR3 alters defense responses in Solanaceae family plants
D'ippólito, Sebastián
Auxin
Biotic Stress
Cladosporium Fulvum
Indole-3-Acetic Acid Amido Hydrolases
Nicotiana Benthamiana
Phytophthora Infestans
Solanum Lycopersicum
title_short Knocking down expression of the auxin-amidohydrolase IAR3 alters defense responses in Solanaceae family plants
title_full Knocking down expression of the auxin-amidohydrolase IAR3 alters defense responses in Solanaceae family plants
title_fullStr Knocking down expression of the auxin-amidohydrolase IAR3 alters defense responses in Solanaceae family plants
title_full_unstemmed Knocking down expression of the auxin-amidohydrolase IAR3 alters defense responses in Solanaceae family plants
title_sort Knocking down expression of the auxin-amidohydrolase IAR3 alters defense responses in Solanaceae family plants
dc.creator.none.fl_str_mv D'ippólito, Sebastián
Vankova, Radomira
Joosten, Matthieu H. A. J.
Casalongue, Claudia
Fiol, Diego Fernando
author D'ippólito, Sebastián
author_facet D'ippólito, Sebastián
Vankova, Radomira
Joosten, Matthieu H. A. J.
Casalongue, Claudia
Fiol, Diego Fernando
author_role author
author2 Vankova, Radomira
Joosten, Matthieu H. A. J.
Casalongue, Claudia
Fiol, Diego Fernando
author2_role author
author
author
author
dc.subject.none.fl_str_mv Auxin
Biotic Stress
Cladosporium Fulvum
Indole-3-Acetic Acid Amido Hydrolases
Nicotiana Benthamiana
Phytophthora Infestans
Solanum Lycopersicum
topic Auxin
Biotic Stress
Cladosporium Fulvum
Indole-3-Acetic Acid Amido Hydrolases
Nicotiana Benthamiana
Phytophthora Infestans
Solanum Lycopersicum
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv In plants, indole-3-acetic acid (IAA) amido hydrolases (AHs) participate in auxin homeostasis by releasing free IAA from IAA-amino acid conjugates. We investigated the role of IAR3, a member of the IAA amido hydrolase family, in the response of Solanaceous plants challenged by biotrophic and hemi-biotrophic pathogens. By means of genome inspection and phylogenic analysis we firstly identified IAA-AH sequences and putative IAR3 orthologs in Nicotiana benthamiana, tomato and potato. We evaluated the involvement of IAR3 genes in defense responses by using virus-induced gene silencing. We observed that N. benthamiana and tomato plants with knocked-down expression of IAR3 genes contained lower levels of free IAA and presented altered responses to pathogen attack, including enhanced basal defenses and higher tolerance to infection in susceptible plants. We showed that IAR3 genes are consistently up-regulated in N. benthamiana and tomato upon inoculation with Phytophthora infestans and Cladosporium fulvum respectively. However, IAR3 expression decreased significantly when hypersensitive response was triggered in transgenic tomato plants coexpressing the Cf-4 resistance gene and the avirulence factor Avr4. Altogether, our results indicate that changes in IAR3 expression lead to alteration in auxin homeostasis that ultimately affects plant defense responses.
Fil: D'ippólito, Sebastián. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Biológicas. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Biológicas; Argentina
Fil: Vankova, Radomira. Academy of Sciences of the Czech Republic; República Checa
Fil: Joosten, Matthieu H. A. J.. Wageningen University; Países Bajos
Fil: Casalongue, Claudia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Biológicas. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Biológicas; Argentina
Fil: Fiol, Diego Fernando. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Biológicas. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Biológicas; Argentina
description In plants, indole-3-acetic acid (IAA) amido hydrolases (AHs) participate in auxin homeostasis by releasing free IAA from IAA-amino acid conjugates. We investigated the role of IAR3, a member of the IAA amido hydrolase family, in the response of Solanaceous plants challenged by biotrophic and hemi-biotrophic pathogens. By means of genome inspection and phylogenic analysis we firstly identified IAA-AH sequences and putative IAR3 orthologs in Nicotiana benthamiana, tomato and potato. We evaluated the involvement of IAR3 genes in defense responses by using virus-induced gene silencing. We observed that N. benthamiana and tomato plants with knocked-down expression of IAR3 genes contained lower levels of free IAA and presented altered responses to pathogen attack, including enhanced basal defenses and higher tolerance to infection in susceptible plants. We showed that IAR3 genes are consistently up-regulated in N. benthamiana and tomato upon inoculation with Phytophthora infestans and Cladosporium fulvum respectively. However, IAR3 expression decreased significantly when hypersensitive response was triggered in transgenic tomato plants coexpressing the Cf-4 resistance gene and the avirulence factor Avr4. Altogether, our results indicate that changes in IAR3 expression lead to alteration in auxin homeostasis that ultimately affects plant defense responses.
publishDate 2016
dc.date.none.fl_str_mv 2016-12
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/62632
D'ippólito, Sebastián; Vankova, Radomira; Joosten, Matthieu H. A. J.; Casalongue, Claudia; Fiol, Diego Fernando; Knocking down expression of the auxin-amidohydrolase IAR3 alters defense responses in Solanaceae family plants; Elsevier Ireland; Plant Science; 253; 12-2016; 31-39
0168-9452
CONICET Digital
CONICET
url http://hdl.handle.net/11336/62632
identifier_str_mv D'ippólito, Sebastián; Vankova, Radomira; Joosten, Matthieu H. A. J.; Casalongue, Claudia; Fiol, Diego Fernando; Knocking down expression of the auxin-amidohydrolase IAR3 alters defense responses in Solanaceae family plants; Elsevier Ireland; Plant Science; 253; 12-2016; 31-39
0168-9452
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0168945216304691
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.plantsci.2016.09.008
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Ireland
publisher.none.fl_str_mv Elsevier Ireland
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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