A comparative study of different microchips for capillary electrophoresis with electrochemical detection

Autores
Erro, Eustaquio Martín; Ortiz, Patricia Ines
Año de publicación
2014
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
This work describes a comparison of the performance of different microchip configuration for microchip capillary electrophoresis with electrochemical detection. Two electrodes gold and multistrand carbon fibers and two microchip construction materials polydimethylsiloxane/polydimethylsiloxane (PDMS/PDMS) and polydimethylsiloxane/glass hybrid (PDMS/glass) were analyzed. The electrode is integrated into the microchip for in-channel triple pulse amperometric detection. Two different mixtures were analyzed (i.e., paracetamol (PA)-4-aminophenol (4-AP) and Dopamine (DO)-Dopac) to demonstrate the electrode and microchips performance. Other variables, such as injection and separation potentials, buffer pH, surfactants addition and injection time, were also analyzed. Hydrodynamic voltammograms were used to select working potential values, and +0.9 V for PA and 4-AP and +0.8 V for DO and dopac were chosen. The migration potential was modified in the 1,500–2,500 V range, and the employed value depends on the microchip materials. The separation process was tested by analyzing the current and migration time variation coefficients. The experimental results demonstrated that the hybrid PDMS/glass microchip with a carbon fiber electrode exhibited a better performance for both samples analyzed.
Fil: Erro, Eustaquio Martín. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Físico-química de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Investigaciones en Físico-química de Córdoba; Argentina
Fil: Ortiz, Patricia Ines. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Físico-química de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Investigaciones en Físico-química de Córdoba; Argentina
Materia
Microchip Capillary Electrophoresis
Gold And Carbon Fiber Electrode
Pdms/Pdms And Pdms/Glass Microchip
Triple Pulse Amperometric Detection
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/31233
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/31233
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling A comparative study of different microchips for capillary electrophoresis with electrochemical detectionErro, Eustaquio MartínOrtiz, Patricia InesMicrochip Capillary ElectrophoresisGold And Carbon Fiber ElectrodePdms/Pdms And Pdms/Glass MicrochipTriple Pulse Amperometric Detectionhttps://purl.org/becyt/ford/1.4https://purl.org/becyt/ford/1This work describes a comparison of the performance of different microchip configuration for microchip capillary electrophoresis with electrochemical detection. Two electrodes gold and multistrand carbon fibers and two microchip construction materials polydimethylsiloxane/polydimethylsiloxane (PDMS/PDMS) and polydimethylsiloxane/glass hybrid (PDMS/glass) were analyzed. The electrode is integrated into the microchip for in-channel triple pulse amperometric detection. Two different mixtures were analyzed (i.e., paracetamol (PA)-4-aminophenol (4-AP) and Dopamine (DO)-Dopac) to demonstrate the electrode and microchips performance. Other variables, such as injection and separation potentials, buffer pH, surfactants addition and injection time, were also analyzed. Hydrodynamic voltammograms were used to select working potential values, and +0.9 V for PA and 4-AP and +0.8 V for DO and dopac were chosen. The migration potential was modified in the 1,500–2,500 V range, and the employed value depends on the microchip materials. The separation process was tested by analyzing the current and migration time variation coefficients. The experimental results demonstrated that the hybrid PDMS/glass microchip with a carbon fiber electrode exhibited a better performance for both samples analyzed.Fil: Erro, Eustaquio Martín. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Físico-química de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Investigaciones en Físico-química de Córdoba; ArgentinaFil: Ortiz, Patricia Ines. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Físico-química de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Investigaciones en Físico-química de Córdoba; ArgentinaSpringer2014-06info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/31233Erro, Eustaquio Martín; Ortiz, Patricia Ines; A comparative study of different microchips for capillary electrophoresis with electrochemical detection; Springer; Journal of Applied Electrochemistry; 44; 12; 6-2014; 1393-13990021-891XCONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1007/s10800-014-0764-1info:eu-repo/semantics/altIdentifier/url/https://link.springer.com/article/10.1007%2Fs10800-014-0764-1info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T14:57:33Zoai:ri.conicet.gov.ar:11336/31233instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 14:57:33.932CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv A comparative study of different microchips for capillary electrophoresis with electrochemical detection
title A comparative study of different microchips for capillary electrophoresis with electrochemical detection
spellingShingle A comparative study of different microchips for capillary electrophoresis with electrochemical detection
Erro, Eustaquio Martín
Microchip Capillary Electrophoresis
Gold And Carbon Fiber Electrode
Pdms/Pdms And Pdms/Glass Microchip
Triple Pulse Amperometric Detection
title_short A comparative study of different microchips for capillary electrophoresis with electrochemical detection
title_full A comparative study of different microchips for capillary electrophoresis with electrochemical detection
title_fullStr A comparative study of different microchips for capillary electrophoresis with electrochemical detection
title_full_unstemmed A comparative study of different microchips for capillary electrophoresis with electrochemical detection
title_sort A comparative study of different microchips for capillary electrophoresis with electrochemical detection
dc.creator.none.fl_str_mv Erro, Eustaquio Martín
Ortiz, Patricia Ines
author Erro, Eustaquio Martín
author_facet Erro, Eustaquio Martín
Ortiz, Patricia Ines
author_role author
author2 Ortiz, Patricia Ines
author2_role author
dc.subject.none.fl_str_mv Microchip Capillary Electrophoresis
Gold And Carbon Fiber Electrode
Pdms/Pdms And Pdms/Glass Microchip
Triple Pulse Amperometric Detection
topic Microchip Capillary Electrophoresis
Gold And Carbon Fiber Electrode
Pdms/Pdms And Pdms/Glass Microchip
Triple Pulse Amperometric Detection
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.4
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv This work describes a comparison of the performance of different microchip configuration for microchip capillary electrophoresis with electrochemical detection. Two electrodes gold and multistrand carbon fibers and two microchip construction materials polydimethylsiloxane/polydimethylsiloxane (PDMS/PDMS) and polydimethylsiloxane/glass hybrid (PDMS/glass) were analyzed. The electrode is integrated into the microchip for in-channel triple pulse amperometric detection. Two different mixtures were analyzed (i.e., paracetamol (PA)-4-aminophenol (4-AP) and Dopamine (DO)-Dopac) to demonstrate the electrode and microchips performance. Other variables, such as injection and separation potentials, buffer pH, surfactants addition and injection time, were also analyzed. Hydrodynamic voltammograms were used to select working potential values, and +0.9 V for PA and 4-AP and +0.8 V for DO and dopac were chosen. The migration potential was modified in the 1,500–2,500 V range, and the employed value depends on the microchip materials. The separation process was tested by analyzing the current and migration time variation coefficients. The experimental results demonstrated that the hybrid PDMS/glass microchip with a carbon fiber electrode exhibited a better performance for both samples analyzed.
Fil: Erro, Eustaquio Martín. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Físico-química de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Investigaciones en Físico-química de Córdoba; Argentina
Fil: Ortiz, Patricia Ines. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Físico-química de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Investigaciones en Físico-química de Córdoba; Argentina
description This work describes a comparison of the performance of different microchip configuration for microchip capillary electrophoresis with electrochemical detection. Two electrodes gold and multistrand carbon fibers and two microchip construction materials polydimethylsiloxane/polydimethylsiloxane (PDMS/PDMS) and polydimethylsiloxane/glass hybrid (PDMS/glass) were analyzed. The electrode is integrated into the microchip for in-channel triple pulse amperometric detection. Two different mixtures were analyzed (i.e., paracetamol (PA)-4-aminophenol (4-AP) and Dopamine (DO)-Dopac) to demonstrate the electrode and microchips performance. Other variables, such as injection and separation potentials, buffer pH, surfactants addition and injection time, were also analyzed. Hydrodynamic voltammograms were used to select working potential values, and +0.9 V for PA and 4-AP and +0.8 V for DO and dopac were chosen. The migration potential was modified in the 1,500–2,500 V range, and the employed value depends on the microchip materials. The separation process was tested by analyzing the current and migration time variation coefficients. The experimental results demonstrated that the hybrid PDMS/glass microchip with a carbon fiber electrode exhibited a better performance for both samples analyzed.
publishDate 2014
dc.date.none.fl_str_mv 2014-06
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/31233
Erro, Eustaquio Martín; Ortiz, Patricia Ines; A comparative study of different microchips for capillary electrophoresis with electrochemical detection; Springer; Journal of Applied Electrochemistry; 44; 12; 6-2014; 1393-1399
0021-891X
CONICET Digital
CONICET
url http://hdl.handle.net/11336/31233
identifier_str_mv Erro, Eustaquio Martín; Ortiz, Patricia Ines; A comparative study of different microchips for capillary electrophoresis with electrochemical detection; Springer; Journal of Applied Electrochemistry; 44; 12; 6-2014; 1393-1399
0021-891X
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1007/s10800-014-0764-1
info:eu-repo/semantics/altIdentifier/url/https://link.springer.com/article/10.1007%2Fs10800-014-0764-1
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Springer
publisher.none.fl_str_mv Springer
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.22299

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