The landscape of Arabidopsis tRNA aminoacylation

• Autores: Ceriotti, Luis Federico; Warren, Jessica M.; Sánchez Puerta, María Virginia; Sloan, Daniel B.
• Año de publicación: 2024
• Idioma: inglés
• Tipo de recurso: artículo
• Materia: MITOCHONDRIA; T-ELEMENTS; POST-TRANSCRIPTIONAL MODIFICATION; ARABIDOPSIS
• Institución: Consejo Nacional de Investigaciones Científicas y Técnicas

The function of transfer RNAs (tRNAs) depends on enzymes that cleave primary transcript ends, add a 30 CCA tail, introduce post-transcriptional base modifications, and charge (aminoacylate) mature tRNAs with the correct amino acid. Maintaining an available pool of the resulting aminoacylated tRNAs is essential for protein synthesis. High-throughput sequencing techniques have recently been developed to provide a com- prehensive view of aminoacylation state in a tRNA-specific fashion. However, these methods have never been applied to plants. Here, we treated Arabidopsis thaliana RNA samples with periodate and then per- formed tRNA-seq to distinguish between aminoacylated and uncharged tRNAs. This approach successfully captured every tRNA isodecoder family and detected expression of additional tRNA-like transcripts. We found that estimated aminoacylation rates and CCA tail integrity were significantly higher on average for organellar (mitochondrial and plastid) tRNAs than for nuclear/cytosolic tRNAs. Reanalysis of previously pub- lished human cell line data showed a similar pattern. Base modifications result in nucleotide misincorpora- tions and truncations during reverse transcription, which we quantified and used to test for relationships with aminoacylation levels. We also determined that the Arabidopsis tRNA-like sequences (t-elements) that are cleaved from the ends of some mitochondrial messenger RNAs have post-transcriptionally modified bases and CCA-tail addition. However, these t-elements are not aminoacylated, indicating that they are only recognized by a subset of tRNA-interacting enzymes and do not play a role in translation. Overall, this work provides a characterization of the baseline landscape of plant tRNA aminoacylation rates and demonstrates an approach for investigating environmental and genetic perturbations to plant translation machinery.
Fil: Ceriotti, Luis Federico. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Biología Agrícola de Mendoza. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza; Argentina
Fil: Warren, Jessica M.. State University of Colorado - Fort Collins; Estados Unidos
Fil: Sánchez Puerta, María Virginia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Biología Agrícola de Mendoza. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza; Argentina
Fil: Sloan, Daniel B.. State University of Colorado - Fort Collins; Estados Unidos