Structural and functional studies of the Mitochondrial Cysteine Desulfurase from Arabidopsis thaliana

Autores
Turowski, Valeria Rosana; Busi, María Victoria; Gomez Casati, Diego Fabian
Año de publicación
2012
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
AtNfs1 is the Arabidopsis thaliana mitochondrial homolog of the bacterial cysteine desulfurases NifS and IscS, having an essential role in cellular Fe–S cluster assembly. Homology modeling of AtNfs1m predicts a high global similarity with E. coli IscS showing a full conservation of residues involved in the catalytic site, whereas the chloroplastic AtNfs2 is more similar to the Synechocystis sp. SufS. Pull-down assays showed that the recombinant mature form, AtNfs1m, specifically binds to Arabidopsis frataxin (AtFH). A hysteretic behavior, with a lag phase of several minutes, was observed and hysteretic parameters were affected by pre-incubation with AtFH. Moreover, AtFH modulates AtNfs1m kinetics, increasing Vmax and decreasing the S0.5 value for cysteine. Results suggest that AtFH plays an important role in the early steps of Fe–S cluster formation by regulating AtNfs1 activity in plant mitochondria.
Fil: Turowski, Valeria Rosana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Centro de Estudios Fotosintéticos y Bioquímicos (i); Argentina. Universidad Nacional de Rosario; Argentina. Universidad Nacional de San Martín; Argentina
Fil: Busi, María Victoria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Centro de Estudios Fotosintéticos y Bioquímicos (i); Argentina. Universidad Nacional de Rosario; Argentina. Universidad Nacional de San Martín; Argentina
Fil: Gomez Casati, Diego Fabian. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Centro de Estudios Fotosintéticos y Bioquímicos (i); Argentina. Universidad Nacional de Rosario; Argentina. Universidad Nacional de San Martín; Argentina
Materia
Cysteine Desulfurase
Fe-S Biogenesis
Mitochondria
Arabidopsis
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/15370
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/15370
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Structural and functional studies of the Mitochondrial Cysteine Desulfurase from Arabidopsis thalianaTurowski, Valeria RosanaBusi, María VictoriaGomez Casati, Diego FabianCysteine DesulfuraseFe-S BiogenesisMitochondriaArabidopsishttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1AtNfs1 is the Arabidopsis thaliana mitochondrial homolog of the bacterial cysteine desulfurases NifS and IscS, having an essential role in cellular Fe–S cluster assembly. Homology modeling of AtNfs1m predicts a high global similarity with E. coli IscS showing a full conservation of residues involved in the catalytic site, whereas the chloroplastic AtNfs2 is more similar to the Synechocystis sp. SufS. Pull-down assays showed that the recombinant mature form, AtNfs1m, specifically binds to Arabidopsis frataxin (AtFH). A hysteretic behavior, with a lag phase of several minutes, was observed and hysteretic parameters were affected by pre-incubation with AtFH. Moreover, AtFH modulates AtNfs1m kinetics, increasing Vmax and decreasing the S0.5 value for cysteine. Results suggest that AtFH plays an important role in the early steps of Fe–S cluster formation by regulating AtNfs1 activity in plant mitochondria.Fil: Turowski, Valeria Rosana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Centro de Estudios Fotosintéticos y Bioquímicos (i); Argentina. Universidad Nacional de Rosario; Argentina. Universidad Nacional de San Martín; ArgentinaFil: Busi, María Victoria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Centro de Estudios Fotosintéticos y Bioquímicos (i); Argentina. Universidad Nacional de Rosario; Argentina. Universidad Nacional de San Martín; ArgentinaFil: Gomez Casati, Diego Fabian. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Centro de Estudios Fotosintéticos y Bioquímicos (i); Argentina. Universidad Nacional de Rosario; Argentina. Universidad Nacional de San Martín; ArgentinaOxford University Press2012-09info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/15370Turowski, Valeria Rosana; Busi, María Victoria; Gomez Casati, Diego Fabian; Structural and functional studies of the Mitochondrial Cysteine Desulfurase from Arabidopsis thaliana; Oxford University Press; Molecular Plant; 5; 5; 9-2012; 1001-10101674-2052enginfo:eu-repo/semantics/altIdentifier/doi/10.1093/mp/sss037info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S167420521460068Xinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T15:29:15Zoai:ri.conicet.gov.ar:11336/15370instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 15:29:16.244CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Structural and functional studies of the Mitochondrial Cysteine Desulfurase from Arabidopsis thaliana
title Structural and functional studies of the Mitochondrial Cysteine Desulfurase from Arabidopsis thaliana
spellingShingle Structural and functional studies of the Mitochondrial Cysteine Desulfurase from Arabidopsis thaliana
Turowski, Valeria Rosana
Cysteine Desulfurase
Fe-S Biogenesis
Mitochondria
Arabidopsis
title_short Structural and functional studies of the Mitochondrial Cysteine Desulfurase from Arabidopsis thaliana
title_full Structural and functional studies of the Mitochondrial Cysteine Desulfurase from Arabidopsis thaliana
title_fullStr Structural and functional studies of the Mitochondrial Cysteine Desulfurase from Arabidopsis thaliana
title_full_unstemmed Structural and functional studies of the Mitochondrial Cysteine Desulfurase from Arabidopsis thaliana
title_sort Structural and functional studies of the Mitochondrial Cysteine Desulfurase from Arabidopsis thaliana
dc.creator.none.fl_str_mv Turowski, Valeria Rosana
Busi, María Victoria
Gomez Casati, Diego Fabian
author Turowski, Valeria Rosana
author_facet Turowski, Valeria Rosana
Busi, María Victoria
Gomez Casati, Diego Fabian
author_role author
author2 Busi, María Victoria
Gomez Casati, Diego Fabian
author2_role author
author
dc.subject.none.fl_str_mv Cysteine Desulfurase
Fe-S Biogenesis
Mitochondria
Arabidopsis
topic Cysteine Desulfurase
Fe-S Biogenesis
Mitochondria
Arabidopsis
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv AtNfs1 is the Arabidopsis thaliana mitochondrial homolog of the bacterial cysteine desulfurases NifS and IscS, having an essential role in cellular Fe–S cluster assembly. Homology modeling of AtNfs1m predicts a high global similarity with E. coli IscS showing a full conservation of residues involved in the catalytic site, whereas the chloroplastic AtNfs2 is more similar to the Synechocystis sp. SufS. Pull-down assays showed that the recombinant mature form, AtNfs1m, specifically binds to Arabidopsis frataxin (AtFH). A hysteretic behavior, with a lag phase of several minutes, was observed and hysteretic parameters were affected by pre-incubation with AtFH. Moreover, AtFH modulates AtNfs1m kinetics, increasing Vmax and decreasing the S0.5 value for cysteine. Results suggest that AtFH plays an important role in the early steps of Fe–S cluster formation by regulating AtNfs1 activity in plant mitochondria.
Fil: Turowski, Valeria Rosana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Centro de Estudios Fotosintéticos y Bioquímicos (i); Argentina. Universidad Nacional de Rosario; Argentina. Universidad Nacional de San Martín; Argentina
Fil: Busi, María Victoria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Centro de Estudios Fotosintéticos y Bioquímicos (i); Argentina. Universidad Nacional de Rosario; Argentina. Universidad Nacional de San Martín; Argentina
Fil: Gomez Casati, Diego Fabian. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Centro de Estudios Fotosintéticos y Bioquímicos (i); Argentina. Universidad Nacional de Rosario; Argentina. Universidad Nacional de San Martín; Argentina
description AtNfs1 is the Arabidopsis thaliana mitochondrial homolog of the bacterial cysteine desulfurases NifS and IscS, having an essential role in cellular Fe–S cluster assembly. Homology modeling of AtNfs1m predicts a high global similarity with E. coli IscS showing a full conservation of residues involved in the catalytic site, whereas the chloroplastic AtNfs2 is more similar to the Synechocystis sp. SufS. Pull-down assays showed that the recombinant mature form, AtNfs1m, specifically binds to Arabidopsis frataxin (AtFH). A hysteretic behavior, with a lag phase of several minutes, was observed and hysteretic parameters were affected by pre-incubation with AtFH. Moreover, AtFH modulates AtNfs1m kinetics, increasing Vmax and decreasing the S0.5 value for cysteine. Results suggest that AtFH plays an important role in the early steps of Fe–S cluster formation by regulating AtNfs1 activity in plant mitochondria.
publishDate 2012
dc.date.none.fl_str_mv 2012-09
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/15370
Turowski, Valeria Rosana; Busi, María Victoria; Gomez Casati, Diego Fabian; Structural and functional studies of the Mitochondrial Cysteine Desulfurase from Arabidopsis thaliana; Oxford University Press; Molecular Plant; 5; 5; 9-2012; 1001-1010
1674-2052
url http://hdl.handle.net/11336/15370
identifier_str_mv Turowski, Valeria Rosana; Busi, María Victoria; Gomez Casati, Diego Fabian; Structural and functional studies of the Mitochondrial Cysteine Desulfurase from Arabidopsis thaliana; Oxford University Press; Molecular Plant; 5; 5; 9-2012; 1001-1010
1674-2052
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1093/mp/sss037
info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S167420521460068X
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Oxford University Press
publisher.none.fl_str_mv Oxford University Press
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.22299

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