Lectin Binding Pattern in the Uterus of Pregnant Mice Infected with Tritrichomonas foetus

Autores
Woudwyk, Mariana Andrea; Gimeno, Eduardo Juan; Soto, Pedro; Barbeito, Claudio Gustavo; Monteavaro, Cristina Esther
Año de publicación
2013
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Bovine genital tritrichomonosis is caused by the protozoon Tritrichomonas foetus and leads to embryonic death and abortion. The complexity of handling bovine experimental systems has led to the development of alternative models. The infection has been reproduced in pregnant BALB/c mice. In the pathogenesis of the disease, adhesion of the protozoon to host cell surface glycoproteins is important. Labelling with soya bean agglutinin (SBA) and peanut agglutinin (PNA) lectins increases in the luminal and glandular uterine epithelium of non-pregnant infected mice. The aim of the present study was to determine whether these changes also occur in pregnant infected BALB/c mice. Female BALB/c mice were inoculated intravaginally with T. foetus and, 15±3days post infection, were paired with males overnight. Infected and control mice were sacrificed 6, 8 and 10days later. Samples of uterus were labelled with a panel of biotinylated lectins. Infected mice showed increased binding of PNA and SBA. There was also increased binding of concanavalin (Con-A) by luminal epithelium and Ricinus communis agglutinin (RCA-1) by glandular epithelium at day 6 post coitum. These changes may be due to the production of enzymes by T. foetus, which could act to enhance adhesion and colonization and thus favour infection.
Fil: Woudwyk, Mariana Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias; Argentina
Fil: Gimeno, Eduardo Juan. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias; Argentina
Fil: Soto, Pedro. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias; Argentina
Fil: Barbeito, Claudio Gustavo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias; Argentina
Fil: Monteavaro, Cristina Esther. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias; Argentina
Materia
Bovine Genital Tritrichomonosis
Lectin Histochemistry
Mouse Model
Pathogenesis
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/76751
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/76751
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Lectin Binding Pattern in the Uterus of Pregnant Mice Infected with Tritrichomonas foetusWoudwyk, Mariana AndreaGimeno, Eduardo JuanSoto, PedroBarbeito, Claudio GustavoMonteavaro, Cristina EstherBovine Genital TritrichomonosisLectin HistochemistryMouse ModelPathogenesishttps://purl.org/becyt/ford/4.3https://purl.org/becyt/ford/4Bovine genital tritrichomonosis is caused by the protozoon Tritrichomonas foetus and leads to embryonic death and abortion. The complexity of handling bovine experimental systems has led to the development of alternative models. The infection has been reproduced in pregnant BALB/c mice. In the pathogenesis of the disease, adhesion of the protozoon to host cell surface glycoproteins is important. Labelling with soya bean agglutinin (SBA) and peanut agglutinin (PNA) lectins increases in the luminal and glandular uterine epithelium of non-pregnant infected mice. The aim of the present study was to determine whether these changes also occur in pregnant infected BALB/c mice. Female BALB/c mice were inoculated intravaginally with T. foetus and, 15±3days post infection, were paired with males overnight. Infected and control mice were sacrificed 6, 8 and 10days later. Samples of uterus were labelled with a panel of biotinylated lectins. Infected mice showed increased binding of PNA and SBA. There was also increased binding of concanavalin (Con-A) by luminal epithelium and Ricinus communis agglutinin (RCA-1) by glandular epithelium at day 6 post coitum. These changes may be due to the production of enzymes by T. foetus, which could act to enhance adhesion and colonization and thus favour infection.Fil: Woudwyk, Mariana Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias; ArgentinaFil: Gimeno, Eduardo Juan. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias; ArgentinaFil: Soto, Pedro. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias; ArgentinaFil: Barbeito, Claudio Gustavo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias; ArgentinaFil: Monteavaro, Cristina Esther. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias; ArgentinaElsevier2013-08info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/76751Woudwyk, Mariana Andrea; Gimeno, Eduardo Juan; Soto, Pedro; Barbeito, Claudio Gustavo; Monteavaro, Cristina Esther; Lectin Binding Pattern in the Uterus of Pregnant Mice Infected with Tritrichomonas foetus; Elsevier; Journal Of Comparative Pathology; 149; 2-3; 8-2013; 341-3450021-9975CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.jcpa.2012.12.004info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0021997512004379info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:11:43Zoai:ri.conicet.gov.ar:11336/76751instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:11:43.561CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Lectin Binding Pattern in the Uterus of Pregnant Mice Infected with Tritrichomonas foetus
title Lectin Binding Pattern in the Uterus of Pregnant Mice Infected with Tritrichomonas foetus
spellingShingle Lectin Binding Pattern in the Uterus of Pregnant Mice Infected with Tritrichomonas foetus
Woudwyk, Mariana Andrea
Bovine Genital Tritrichomonosis
Lectin Histochemistry
Mouse Model
Pathogenesis
title_short Lectin Binding Pattern in the Uterus of Pregnant Mice Infected with Tritrichomonas foetus
title_full Lectin Binding Pattern in the Uterus of Pregnant Mice Infected with Tritrichomonas foetus
title_fullStr Lectin Binding Pattern in the Uterus of Pregnant Mice Infected with Tritrichomonas foetus
title_full_unstemmed Lectin Binding Pattern in the Uterus of Pregnant Mice Infected with Tritrichomonas foetus
title_sort Lectin Binding Pattern in the Uterus of Pregnant Mice Infected with Tritrichomonas foetus
dc.creator.none.fl_str_mv Woudwyk, Mariana Andrea
Gimeno, Eduardo Juan
Soto, Pedro
Barbeito, Claudio Gustavo
Monteavaro, Cristina Esther
author Woudwyk, Mariana Andrea
author_facet Woudwyk, Mariana Andrea
Gimeno, Eduardo Juan
Soto, Pedro
Barbeito, Claudio Gustavo
Monteavaro, Cristina Esther
author_role author
author2 Gimeno, Eduardo Juan
Soto, Pedro
Barbeito, Claudio Gustavo
Monteavaro, Cristina Esther
author2_role author
author
author
author
dc.subject.none.fl_str_mv Bovine Genital Tritrichomonosis
Lectin Histochemistry
Mouse Model
Pathogenesis
topic Bovine Genital Tritrichomonosis
Lectin Histochemistry
Mouse Model
Pathogenesis
purl_subject.fl_str_mv https://purl.org/becyt/ford/4.3
https://purl.org/becyt/ford/4
dc.description.none.fl_txt_mv Bovine genital tritrichomonosis is caused by the protozoon Tritrichomonas foetus and leads to embryonic death and abortion. The complexity of handling bovine experimental systems has led to the development of alternative models. The infection has been reproduced in pregnant BALB/c mice. In the pathogenesis of the disease, adhesion of the protozoon to host cell surface glycoproteins is important. Labelling with soya bean agglutinin (SBA) and peanut agglutinin (PNA) lectins increases in the luminal and glandular uterine epithelium of non-pregnant infected mice. The aim of the present study was to determine whether these changes also occur in pregnant infected BALB/c mice. Female BALB/c mice were inoculated intravaginally with T. foetus and, 15±3days post infection, were paired with males overnight. Infected and control mice were sacrificed 6, 8 and 10days later. Samples of uterus were labelled with a panel of biotinylated lectins. Infected mice showed increased binding of PNA and SBA. There was also increased binding of concanavalin (Con-A) by luminal epithelium and Ricinus communis agglutinin (RCA-1) by glandular epithelium at day 6 post coitum. These changes may be due to the production of enzymes by T. foetus, which could act to enhance adhesion and colonization and thus favour infection.
Fil: Woudwyk, Mariana Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias; Argentina
Fil: Gimeno, Eduardo Juan. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias; Argentina
Fil: Soto, Pedro. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias; Argentina
Fil: Barbeito, Claudio Gustavo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias; Argentina
Fil: Monteavaro, Cristina Esther. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias; Argentina
description Bovine genital tritrichomonosis is caused by the protozoon Tritrichomonas foetus and leads to embryonic death and abortion. The complexity of handling bovine experimental systems has led to the development of alternative models. The infection has been reproduced in pregnant BALB/c mice. In the pathogenesis of the disease, adhesion of the protozoon to host cell surface glycoproteins is important. Labelling with soya bean agglutinin (SBA) and peanut agglutinin (PNA) lectins increases in the luminal and glandular uterine epithelium of non-pregnant infected mice. The aim of the present study was to determine whether these changes also occur in pregnant infected BALB/c mice. Female BALB/c mice were inoculated intravaginally with T. foetus and, 15±3days post infection, were paired with males overnight. Infected and control mice were sacrificed 6, 8 and 10days later. Samples of uterus were labelled with a panel of biotinylated lectins. Infected mice showed increased binding of PNA and SBA. There was also increased binding of concanavalin (Con-A) by luminal epithelium and Ricinus communis agglutinin (RCA-1) by glandular epithelium at day 6 post coitum. These changes may be due to the production of enzymes by T. foetus, which could act to enhance adhesion and colonization and thus favour infection.
publishDate 2013
dc.date.none.fl_str_mv 2013-08
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/76751
Woudwyk, Mariana Andrea; Gimeno, Eduardo Juan; Soto, Pedro; Barbeito, Claudio Gustavo; Monteavaro, Cristina Esther; Lectin Binding Pattern in the Uterus of Pregnant Mice Infected with Tritrichomonas foetus; Elsevier; Journal Of Comparative Pathology; 149; 2-3; 8-2013; 341-345
0021-9975
CONICET Digital
CONICET
url http://hdl.handle.net/11336/76751
identifier_str_mv Woudwyk, Mariana Andrea; Gimeno, Eduardo Juan; Soto, Pedro; Barbeito, Claudio Gustavo; Monteavaro, Cristina Esther; Lectin Binding Pattern in the Uterus of Pregnant Mice Infected with Tritrichomonas foetus; Elsevier; Journal Of Comparative Pathology; 149; 2-3; 8-2013; 341-345
0021-9975
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1016/j.jcpa.2012.12.004
info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0021997512004379
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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