Lectin binding pattern and immunohistochemical antigen detection in the genitalia of Tritrichomonas foetus-infected heifers
- Autores
- Cobo, Eduardo Ruben; Campero, Carlos Manuel; Gimeno, Eduardo Juan; Barbeito, Claudio Gustavo
- Año de publicación
- 2004
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- Heifers inoculated intra-vaginally with Tritrichomonas foetus were examined after long-term infection (70 days) and short-term infection (20 days) by lectin-histochemical, immunohistochemical and cultural techniques. The organism was recovered from the genital tract and T. foetus antigens were detected immunohistochemically in the lumina of uterine glands and cytoplasm of vaginal subepithelial macrophages. An increase of galactosylated residues (galactose and N-acetyl galactose), binding to PNA, was observed in the genital epithelium (vagina, uterus and oviduct) from infected animals. In the oviductal epithelium of short- but not long-term infected heifers, mannose (binding to Con A) was detected, suggesting that the persistent presence of T. foetus and its virulence factors or inflammatory processes result in a change in the glycoproteins of the epithelial surface. The findings have implications for the adhesion of T. foetus to cells and for the pathogenesis of bovine trichomonosis.
Fil: Cobo, Eduardo Ruben. Instituto Nacional de Tecnología Agropecuaria; Argentina
Fil: Campero, Carlos Manuel. Instituto Nacional de Tecnología Agropecuaria; Argentina
Fil: Gimeno, Eduardo Juan. Universidad Nacional de La Plata; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Barbeito, Claudio Gustavo. Universidad Nacional de La Plata; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina - Materia
-
Tritrichomonas Foetus
Bovine Genital Tract
Lectin
Immunohistochemistry - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
- Repositorio
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/30461
Ver los metadatos del registro completo
id |
CONICETDig_fe188575611eaf494b1fea6c6aa41549 |
---|---|
oai_identifier_str |
oai:ri.conicet.gov.ar:11336/30461 |
network_acronym_str |
CONICETDig |
repository_id_str |
3498 |
network_name_str |
CONICET Digital (CONICET) |
spelling |
Lectin binding pattern and immunohistochemical antigen detection in the genitalia of Tritrichomonas foetus-infected heifersCobo, Eduardo RubenCampero, Carlos ManuelGimeno, Eduardo JuanBarbeito, Claudio GustavoTritrichomonas FoetusBovine Genital TractLectinImmunohistochemistryHeifers inoculated intra-vaginally with Tritrichomonas foetus were examined after long-term infection (70 days) and short-term infection (20 days) by lectin-histochemical, immunohistochemical and cultural techniques. The organism was recovered from the genital tract and T. foetus antigens were detected immunohistochemically in the lumina of uterine glands and cytoplasm of vaginal subepithelial macrophages. An increase of galactosylated residues (galactose and N-acetyl galactose), binding to PNA, was observed in the genital epithelium (vagina, uterus and oviduct) from infected animals. In the oviductal epithelium of short- but not long-term infected heifers, mannose (binding to Con A) was detected, suggesting that the persistent presence of T. foetus and its virulence factors or inflammatory processes result in a change in the glycoproteins of the epithelial surface. The findings have implications for the adhesion of T. foetus to cells and for the pathogenesis of bovine trichomonosis.Fil: Cobo, Eduardo Ruben. Instituto Nacional de Tecnología Agropecuaria; ArgentinaFil: Campero, Carlos Manuel. Instituto Nacional de Tecnología Agropecuaria; ArgentinaFil: Gimeno, Eduardo Juan. Universidad Nacional de La Plata; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Barbeito, Claudio Gustavo. Universidad Nacional de La Plata; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaElsevier2004-08info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/30461Cobo, Eduardo Ruben; Campero, Carlos Manuel; Gimeno, Eduardo Juan; Barbeito, Claudio Gustavo; Lectin binding pattern and immunohistochemical antigen detection in the genitalia of Tritrichomonas foetus-infected heifers; Elsevier; Journal Of Comparative Pathology; 131; 2; 8-2004; 127-1340021-9975CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://www.ncbi.nlm.nih.gov/pubmed/15276852info:eu-repo/semantics/altIdentifier/doi/ 10.1016/j.jcpa.2004.02.003info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:47:04Zoai:ri.conicet.gov.ar:11336/30461instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:47:04.442CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
dc.title.none.fl_str_mv |
Lectin binding pattern and immunohistochemical antigen detection in the genitalia of Tritrichomonas foetus-infected heifers |
title |
Lectin binding pattern and immunohistochemical antigen detection in the genitalia of Tritrichomonas foetus-infected heifers |
spellingShingle |
Lectin binding pattern and immunohistochemical antigen detection in the genitalia of Tritrichomonas foetus-infected heifers Cobo, Eduardo Ruben Tritrichomonas Foetus Bovine Genital Tract Lectin Immunohistochemistry |
title_short |
Lectin binding pattern and immunohistochemical antigen detection in the genitalia of Tritrichomonas foetus-infected heifers |
title_full |
Lectin binding pattern and immunohistochemical antigen detection in the genitalia of Tritrichomonas foetus-infected heifers |
title_fullStr |
Lectin binding pattern and immunohistochemical antigen detection in the genitalia of Tritrichomonas foetus-infected heifers |
title_full_unstemmed |
Lectin binding pattern and immunohistochemical antigen detection in the genitalia of Tritrichomonas foetus-infected heifers |
title_sort |
Lectin binding pattern and immunohistochemical antigen detection in the genitalia of Tritrichomonas foetus-infected heifers |
dc.creator.none.fl_str_mv |
Cobo, Eduardo Ruben Campero, Carlos Manuel Gimeno, Eduardo Juan Barbeito, Claudio Gustavo |
author |
Cobo, Eduardo Ruben |
author_facet |
Cobo, Eduardo Ruben Campero, Carlos Manuel Gimeno, Eduardo Juan Barbeito, Claudio Gustavo |
author_role |
author |
author2 |
Campero, Carlos Manuel Gimeno, Eduardo Juan Barbeito, Claudio Gustavo |
author2_role |
author author author |
dc.subject.none.fl_str_mv |
Tritrichomonas Foetus Bovine Genital Tract Lectin Immunohistochemistry |
topic |
Tritrichomonas Foetus Bovine Genital Tract Lectin Immunohistochemistry |
dc.description.none.fl_txt_mv |
Heifers inoculated intra-vaginally with Tritrichomonas foetus were examined after long-term infection (70 days) and short-term infection (20 days) by lectin-histochemical, immunohistochemical and cultural techniques. The organism was recovered from the genital tract and T. foetus antigens were detected immunohistochemically in the lumina of uterine glands and cytoplasm of vaginal subepithelial macrophages. An increase of galactosylated residues (galactose and N-acetyl galactose), binding to PNA, was observed in the genital epithelium (vagina, uterus and oviduct) from infected animals. In the oviductal epithelium of short- but not long-term infected heifers, mannose (binding to Con A) was detected, suggesting that the persistent presence of T. foetus and its virulence factors or inflammatory processes result in a change in the glycoproteins of the epithelial surface. The findings have implications for the adhesion of T. foetus to cells and for the pathogenesis of bovine trichomonosis. Fil: Cobo, Eduardo Ruben. Instituto Nacional de Tecnología Agropecuaria; Argentina Fil: Campero, Carlos Manuel. Instituto Nacional de Tecnología Agropecuaria; Argentina Fil: Gimeno, Eduardo Juan. Universidad Nacional de La Plata; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Barbeito, Claudio Gustavo. Universidad Nacional de La Plata; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina |
description |
Heifers inoculated intra-vaginally with Tritrichomonas foetus were examined after long-term infection (70 days) and short-term infection (20 days) by lectin-histochemical, immunohistochemical and cultural techniques. The organism was recovered from the genital tract and T. foetus antigens were detected immunohistochemically in the lumina of uterine glands and cytoplasm of vaginal subepithelial macrophages. An increase of galactosylated residues (galactose and N-acetyl galactose), binding to PNA, was observed in the genital epithelium (vagina, uterus and oviduct) from infected animals. In the oviductal epithelium of short- but not long-term infected heifers, mannose (binding to Con A) was detected, suggesting that the persistent presence of T. foetus and its virulence factors or inflammatory processes result in a change in the glycoproteins of the epithelial surface. The findings have implications for the adhesion of T. foetus to cells and for the pathogenesis of bovine trichomonosis. |
publishDate |
2004 |
dc.date.none.fl_str_mv |
2004-08 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/30461 Cobo, Eduardo Ruben; Campero, Carlos Manuel; Gimeno, Eduardo Juan; Barbeito, Claudio Gustavo; Lectin binding pattern and immunohistochemical antigen detection in the genitalia of Tritrichomonas foetus-infected heifers; Elsevier; Journal Of Comparative Pathology; 131; 2; 8-2004; 127-134 0021-9975 CONICET Digital CONICET |
url |
http://hdl.handle.net/11336/30461 |
identifier_str_mv |
Cobo, Eduardo Ruben; Campero, Carlos Manuel; Gimeno, Eduardo Juan; Barbeito, Claudio Gustavo; Lectin binding pattern and immunohistochemical antigen detection in the genitalia of Tritrichomonas foetus-infected heifers; Elsevier; Journal Of Comparative Pathology; 131; 2; 8-2004; 127-134 0021-9975 CONICET Digital CONICET |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/url/http://www.ncbi.nlm.nih.gov/pubmed/15276852 info:eu-repo/semantics/altIdentifier/doi/ 10.1016/j.jcpa.2004.02.003 |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
dc.format.none.fl_str_mv |
application/pdf application/pdf application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
Elsevier |
publisher.none.fl_str_mv |
Elsevier |
dc.source.none.fl_str_mv |
reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas |
reponame_str |
CONICET Digital (CONICET) |
collection |
CONICET Digital (CONICET) |
instname_str |
Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.name.fl_str_mv |
CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.mail.fl_str_mv |
dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
_version_ |
1844614513838522368 |
score |
13.070432 |