Lectin binding pattern and immunohistochemical antigen detection in the genitalia of Tritrichomonas foetus-infected heifers

Autores
Cobo, Eduardo Ruben; Campero, Carlos Manuel; Gimeno, Eduardo Juan; Barbeito, Claudio Gustavo
Año de publicación
2004
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Heifers inoculated intra-vaginally with Tritrichomonas foetus were examined after long-term infection (70 days) and short-term infection (20 days) by lectin-histochemical, immunohistochemical and cultural techniques. The organism was recovered from the genital tract and T. foetus antigens were detected immunohistochemically in the lumina of uterine glands and cytoplasm of vaginal subepithelial macrophages. An increase of galactosylated residues (galactose and N-acetyl galactose), binding to PNA, was observed in the genital epithelium (vagina, uterus and oviduct) from infected animals. In the oviductal epithelium of short- but not long-term infected heifers, mannose (binding to Con A) was detected, suggesting that the persistent presence of T. foetus and its virulence factors or inflammatory processes result in a change in the glycoproteins of the epithelial surface. The findings have implications for the adhesion of T. foetus to cells and for the pathogenesis of bovine trichomonosis.
Fil: Cobo, Eduardo Ruben. Instituto Nacional de Tecnología Agropecuaria; Argentina
Fil: Campero, Carlos Manuel. Instituto Nacional de Tecnología Agropecuaria; Argentina
Fil: Gimeno, Eduardo Juan. Universidad Nacional de La Plata; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Barbeito, Claudio Gustavo. Universidad Nacional de La Plata; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Materia
Tritrichomonas Foetus
Bovine Genital Tract
Lectin
Immunohistochemistry
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/30461
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/30461
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Lectin binding pattern and immunohistochemical antigen detection in the genitalia of Tritrichomonas foetus-infected heifersCobo, Eduardo RubenCampero, Carlos ManuelGimeno, Eduardo JuanBarbeito, Claudio GustavoTritrichomonas FoetusBovine Genital TractLectinImmunohistochemistryHeifers inoculated intra-vaginally with Tritrichomonas foetus were examined after long-term infection (70 days) and short-term infection (20 days) by lectin-histochemical, immunohistochemical and cultural techniques. The organism was recovered from the genital tract and T. foetus antigens were detected immunohistochemically in the lumina of uterine glands and cytoplasm of vaginal subepithelial macrophages. An increase of galactosylated residues (galactose and N-acetyl galactose), binding to PNA, was observed in the genital epithelium (vagina, uterus and oviduct) from infected animals. In the oviductal epithelium of short- but not long-term infected heifers, mannose (binding to Con A) was detected, suggesting that the persistent presence of T. foetus and its virulence factors or inflammatory processes result in a change in the glycoproteins of the epithelial surface. The findings have implications for the adhesion of T. foetus to cells and for the pathogenesis of bovine trichomonosis.Fil: Cobo, Eduardo Ruben. Instituto Nacional de Tecnología Agropecuaria; ArgentinaFil: Campero, Carlos Manuel. Instituto Nacional de Tecnología Agropecuaria; ArgentinaFil: Gimeno, Eduardo Juan. Universidad Nacional de La Plata; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Barbeito, Claudio Gustavo. Universidad Nacional de La Plata; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaElsevier2004-08info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/30461Cobo, Eduardo Ruben; Campero, Carlos Manuel; Gimeno, Eduardo Juan; Barbeito, Claudio Gustavo; Lectin binding pattern and immunohistochemical antigen detection in the genitalia of Tritrichomonas foetus-infected heifers; Elsevier; Journal Of Comparative Pathology; 131; 2; 8-2004; 127-1340021-9975CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://www.ncbi.nlm.nih.gov/pubmed/15276852info:eu-repo/semantics/altIdentifier/doi/ 10.1016/j.jcpa.2004.02.003info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-22T12:20:28Zoai:ri.conicet.gov.ar:11336/30461instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-22 12:20:28.867CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Lectin binding pattern and immunohistochemical antigen detection in the genitalia of Tritrichomonas foetus-infected heifers
title Lectin binding pattern and immunohistochemical antigen detection in the genitalia of Tritrichomonas foetus-infected heifers
spellingShingle Lectin binding pattern and immunohistochemical antigen detection in the genitalia of Tritrichomonas foetus-infected heifers
Cobo, Eduardo Ruben
Tritrichomonas Foetus
Bovine Genital Tract
Lectin
Immunohistochemistry
title_short Lectin binding pattern and immunohistochemical antigen detection in the genitalia of Tritrichomonas foetus-infected heifers
title_full Lectin binding pattern and immunohistochemical antigen detection in the genitalia of Tritrichomonas foetus-infected heifers
title_fullStr Lectin binding pattern and immunohistochemical antigen detection in the genitalia of Tritrichomonas foetus-infected heifers
title_full_unstemmed Lectin binding pattern and immunohistochemical antigen detection in the genitalia of Tritrichomonas foetus-infected heifers
title_sort Lectin binding pattern and immunohistochemical antigen detection in the genitalia of Tritrichomonas foetus-infected heifers
dc.creator.none.fl_str_mv Cobo, Eduardo Ruben
Campero, Carlos Manuel
Gimeno, Eduardo Juan
Barbeito, Claudio Gustavo
author Cobo, Eduardo Ruben
author_facet Cobo, Eduardo Ruben
Campero, Carlos Manuel
Gimeno, Eduardo Juan
Barbeito, Claudio Gustavo
author_role author
author2 Campero, Carlos Manuel
Gimeno, Eduardo Juan
Barbeito, Claudio Gustavo
author2_role author
author
author
dc.subject.none.fl_str_mv Tritrichomonas Foetus
Bovine Genital Tract
Lectin
Immunohistochemistry
topic Tritrichomonas Foetus
Bovine Genital Tract
Lectin
Immunohistochemistry
dc.description.none.fl_txt_mv Heifers inoculated intra-vaginally with Tritrichomonas foetus were examined after long-term infection (70 days) and short-term infection (20 days) by lectin-histochemical, immunohistochemical and cultural techniques. The organism was recovered from the genital tract and T. foetus antigens were detected immunohistochemically in the lumina of uterine glands and cytoplasm of vaginal subepithelial macrophages. An increase of galactosylated residues (galactose and N-acetyl galactose), binding to PNA, was observed in the genital epithelium (vagina, uterus and oviduct) from infected animals. In the oviductal epithelium of short- but not long-term infected heifers, mannose (binding to Con A) was detected, suggesting that the persistent presence of T. foetus and its virulence factors or inflammatory processes result in a change in the glycoproteins of the epithelial surface. The findings have implications for the adhesion of T. foetus to cells and for the pathogenesis of bovine trichomonosis.
Fil: Cobo, Eduardo Ruben. Instituto Nacional de Tecnología Agropecuaria; Argentina
Fil: Campero, Carlos Manuel. Instituto Nacional de Tecnología Agropecuaria; Argentina
Fil: Gimeno, Eduardo Juan. Universidad Nacional de La Plata; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Barbeito, Claudio Gustavo. Universidad Nacional de La Plata; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
description Heifers inoculated intra-vaginally with Tritrichomonas foetus were examined after long-term infection (70 days) and short-term infection (20 days) by lectin-histochemical, immunohistochemical and cultural techniques. The organism was recovered from the genital tract and T. foetus antigens were detected immunohistochemically in the lumina of uterine glands and cytoplasm of vaginal subepithelial macrophages. An increase of galactosylated residues (galactose and N-acetyl galactose), binding to PNA, was observed in the genital epithelium (vagina, uterus and oviduct) from infected animals. In the oviductal epithelium of short- but not long-term infected heifers, mannose (binding to Con A) was detected, suggesting that the persistent presence of T. foetus and its virulence factors or inflammatory processes result in a change in the glycoproteins of the epithelial surface. The findings have implications for the adhesion of T. foetus to cells and for the pathogenesis of bovine trichomonosis.
publishDate 2004
dc.date.none.fl_str_mv 2004-08
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/30461
Cobo, Eduardo Ruben; Campero, Carlos Manuel; Gimeno, Eduardo Juan; Barbeito, Claudio Gustavo; Lectin binding pattern and immunohistochemical antigen detection in the genitalia of Tritrichomonas foetus-infected heifers; Elsevier; Journal Of Comparative Pathology; 131; 2; 8-2004; 127-134
0021-9975
CONICET Digital
CONICET
url http://hdl.handle.net/11336/30461
identifier_str_mv Cobo, Eduardo Ruben; Campero, Carlos Manuel; Gimeno, Eduardo Juan; Barbeito, Claudio Gustavo; Lectin binding pattern and immunohistochemical antigen detection in the genitalia of Tritrichomonas foetus-infected heifers; Elsevier; Journal Of Comparative Pathology; 131; 2; 8-2004; 127-134
0021-9975
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://www.ncbi.nlm.nih.gov/pubmed/15276852
info:eu-repo/semantics/altIdentifier/doi/ 10.1016/j.jcpa.2004.02.003
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1846782652225421312
score 13.199325

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