A comparative pattern of lectin-binding in the endometrial glands of the uterus and placenta of healthy buffaloes and bovines at early gestation

• Autores: Caspe, Sergio Gastón; Konrad, José Luis; Moore, Dadin Prando; Sala, Juan Manuel; Lischinsky, Lilian; Campero, Carlos Manuel; Barbeito, Claudio Gustavo
• Año de publicación: 2021
• Idioma: inglés
• Tipo de recurso: artículo
• Fuente: Biocell, 2021, vol. 45, no. 2, p. 331-337.
• Materia: Lectin-histochemistry; Water buffalo; Placenta; Glycosylation pattern
• Institución: Universidad Nacional del Nordeste

Fil: Caspe, Sergio Gastón. Instituto Nacional de Tecnología Agropecuaria. Experimental Station Mercedes. Laboratory of Pathology; Argentina.
Fil: Konrad, José Luis. Universidad Nacional del Nordeste. Facultad de Ciencias Veterinarias; Argentina.
Fil: Moore, Dadin Prando. Instituto Nacional de Tecnología Agropecuaria. Experimental Station Balcarce. Animal Health Group; Argentina.
Fil: Sala, Juan Manuel. Instituto Nacional de Tecnología Agropecuaria. Experimental Station Mercedes. Laboratory of Pathology; Argentina.
Fil: Lischinsky, Lilian. Instituto Nacional de Tecnología Agropecuaria. Experimental Station Balcarce. Animal Health Group; Argentina.
Fil: Campero, Carlos Manuel. Instituto Nacional de Tecnología Agropecuaria. Experimental Station Balcarce. Animal Health Group; Argentina.
Fil: Barbeito, Claudio Gustavo. Universidad Nacional de La Plata. School of Veterinary Sciences. Laboratory of Histology and Embryology; Argentina.
Water buffalo (Bubalus bubalis) and domestic cattle (Bos taurus) are closely related species. However, embryo transfer interspecies has been attempted without any success. The failure in hybrid embryo-implantation is associated with the glycocode in the maternal-fetal interface. Glycosylation patterns have been studied in different species of ruminants; however, in B. bubalis, only the binucleated cells (BNC) have been analyzed. This glycocode is essential for a successful embryo-implantation and can be defined by Lectin-Histochemistry (LHC). The aim of this study is to compare the glycosylation pattern of placenta and uterus in water buffaloes and cattle by LHC. Tissue samples of placenta and uterus from pregnant Mediterranean female water buffaloes (Buf1) and Angus cows (Bov1) were analyzed. All animals were euthanized at 98 days of gestation. LHC was carried out using twelve lectins (Con A, LCA, PSA, sWGA, PHA-e, SBA, UEA-1, WGA, RCA-1, PNA, DBA, BSA-1). The intensity of lectin binding was semi- quantitatively scored using a scale of 0 (negative) to 3 (strongly positive). Difference between species was found in trophoblast layer by PSA, SWGA, PNA and BSA-1, in BNC, and in the mononuclear cells by LCA, PSA, PHA-e, DBA, BSA-1, PNA. In utero, differences in the apical cellular membrane and the secretion of glands were identified by DBA and RCA-1, and in the cytoplasm of those glandular epithelial cells by PHA-e, BSA-1, WGA, and SBA. In both species, BNC presented a strong positive reaction with DBA and SBA, a moderate response by LCA, PHA-e, BSA-1 and PNA lectin, and a low reaction by PSA, UEA-1, SWGA, WGA, Con A and RCA-1. The results found in this study suggest that although both species are closely related, glycosylation patterns in the placenta and uterus are different, thus providing a possible reason for embryo transfer not being possible between these species.