A comparative pattern of lectin-binding in the endometrial glands of the uterus and placenta of healthy buffaloes and bovines at early gestation

Autores
Caspe, Sergio Gastón; Konrad, José Luis; Moore, Dadin Prando; Sala, Juan Manuel; Lischinsky, Lilian; Campero, Carlos Manuel; Barbeito, Claudio Gustavo
Año de publicación
2021
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Fil: Caspe, Sergio Gastón. Instituto Nacional de Tecnología Agropecuaria. Experimental Station Mercedes. Laboratory of Pathology; Argentina.
Fil: Konrad, José Luis. Universidad Nacional del Nordeste. Facultad de Ciencias Veterinarias; Argentina.
Fil: Moore, Dadin Prando. Instituto Nacional de Tecnología Agropecuaria. Experimental Station Balcarce. Animal Health Group; Argentina.
Fil: Sala, Juan Manuel. Instituto Nacional de Tecnología Agropecuaria. Experimental Station Mercedes. Laboratory of Pathology; Argentina.
Fil: Lischinsky, Lilian. Instituto Nacional de Tecnología Agropecuaria. Experimental Station Balcarce. Animal Health Group; Argentina.
Fil: Campero, Carlos Manuel. Instituto Nacional de Tecnología Agropecuaria. Experimental Station Balcarce. Animal Health Group; Argentina.
Fil: Barbeito, Claudio Gustavo. Universidad Nacional de La Plata. School of Veterinary Sciences. Laboratory of Histology and Embryology; Argentina.
Water buffalo (Bubalus bubalis) and domestic cattle (Bos taurus) are closely related species. However, embryo transfer interspecies has been attempted without any success. The failure in hybrid embryo-implantation is associated with the glycocode in the maternal-fetal interface. Glycosylation patterns have been studied in different species of ruminants; however, in B. bubalis, only the binucleated cells (BNC) have been analyzed. This glycocode is essential for a successful embryo-implantation and can be defined by Lectin-Histochemistry (LHC). The aim of this study is to compare the glycosylation pattern of placenta and uterus in water buffaloes and cattle by LHC. Tissue samples of placenta and uterus from pregnant Mediterranean female water buffaloes (Buf1) and Angus cows (Bov1) were analyzed. All animals were euthanized at 98 days of gestation. LHC was carried out using twelve lectins (Con A, LCA, PSA, sWGA, PHA-e, SBA, UEA-1, WGA, RCA-1, PNA, DBA, BSA-1). The intensity of lectin binding was semi- quantitatively scored using a scale of 0 (negative) to 3 (strongly positive). Difference between species was found in trophoblast layer by PSA, SWGA, PNA and BSA-1, in BNC, and in the mononuclear cells by LCA, PSA, PHA-e, DBA, BSA-1, PNA. In utero, differences in the apical cellular membrane and the secretion of glands were identified by DBA and RCA-1, and in the cytoplasm of those glandular epithelial cells by PHA-e, BSA-1, WGA, and SBA. In both species, BNC presented a strong positive reaction with DBA and SBA, a moderate response by LCA, PHA-e, BSA-1 and PNA lectin, and a low reaction by PSA, UEA-1, SWGA, WGA, Con A and RCA-1. The results found in this study suggest that although both species are closely related, glycosylation patterns in the placenta and uterus are different, thus providing a possible reason for embryo transfer not being possible between these species.
Fuente
Biocell, 2021, vol. 45, no. 2, p. 331-337.
Materia
Lectin-histochemistry
Water buffalo
Placenta
Glycosylation pattern
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
Repositorio Institucional de la Universidad Nacional del Nordeste (UNNE)
Institución
Universidad Nacional del Nordeste
OAI Identificador
oai:repositorio.unne.edu.ar:123456789/53157

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network_acronym_str RIUNNE
repository_id_str 4871
network_name_str Repositorio Institucional de la Universidad Nacional del Nordeste (UNNE)
spelling A comparative pattern of lectin-binding in the endometrial glands of the uterus and placenta of healthy buffaloes and bovines at early gestationCaspe, Sergio GastónKonrad, José LuisMoore, Dadin PrandoSala, Juan ManuelLischinsky, LilianCampero, Carlos ManuelBarbeito, Claudio GustavoLectin-histochemistryWater buffaloPlacentaGlycosylation patternFil: Caspe, Sergio Gastón. Instituto Nacional de Tecnología Agropecuaria. Experimental Station Mercedes. Laboratory of Pathology; Argentina.Fil: Konrad, José Luis. Universidad Nacional del Nordeste. Facultad de Ciencias Veterinarias; Argentina.Fil: Moore, Dadin Prando. Instituto Nacional de Tecnología Agropecuaria. Experimental Station Balcarce. Animal Health Group; Argentina.Fil: Sala, Juan Manuel. Instituto Nacional de Tecnología Agropecuaria. Experimental Station Mercedes. Laboratory of Pathology; Argentina.Fil: Lischinsky, Lilian. Instituto Nacional de Tecnología Agropecuaria. Experimental Station Balcarce. Animal Health Group; Argentina.Fil: Campero, Carlos Manuel. Instituto Nacional de Tecnología Agropecuaria. Experimental Station Balcarce. Animal Health Group; Argentina.Fil: Barbeito, Claudio Gustavo. Universidad Nacional de La Plata. School of Veterinary Sciences. Laboratory of Histology and Embryology; Argentina.Water buffalo (Bubalus bubalis) and domestic cattle (Bos taurus) are closely related species. However, embryo transfer interspecies has been attempted without any success. The failure in hybrid embryo-implantation is associated with the glycocode in the maternal-fetal interface. Glycosylation patterns have been studied in different species of ruminants; however, in B. bubalis, only the binucleated cells (BNC) have been analyzed. This glycocode is essential for a successful embryo-implantation and can be defined by Lectin-Histochemistry (LHC). The aim of this study is to compare the glycosylation pattern of placenta and uterus in water buffaloes and cattle by LHC. Tissue samples of placenta and uterus from pregnant Mediterranean female water buffaloes (Buf1) and Angus cows (Bov1) were analyzed. All animals were euthanized at 98 days of gestation. LHC was carried out using twelve lectins (Con A, LCA, PSA, sWGA, PHA-e, SBA, UEA-1, WGA, RCA-1, PNA, DBA, BSA-1). The intensity of lectin binding was semi- quantitatively scored using a scale of 0 (negative) to 3 (strongly positive). Difference between species was found in trophoblast layer by PSA, SWGA, PNA and BSA-1, in BNC, and in the mononuclear cells by LCA, PSA, PHA-e, DBA, BSA-1, PNA. In utero, differences in the apical cellular membrane and the secretion of glands were identified by DBA and RCA-1, and in the cytoplasm of those glandular epithelial cells by PHA-e, BSA-1, WGA, and SBA. In both species, BNC presented a strong positive reaction with DBA and SBA, a moderate response by LCA, PHA-e, BSA-1 and PNA lectin, and a low reaction by PSA, UEA-1, SWGA, WGA, Con A and RCA-1. The results found in this study suggest that although both species are closely related, glycosylation patterns in the placenta and uterus are different, thus providing a possible reason for embryo transfer not being possible between these species.Instituto de Histología y Embriología2021info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfp. 331-337application/pdfCaspe, Sergio Gastón, et al., 2021. A comparative pattern of lectin-binding in the endometrial glands of the uterus and placenta of healthy buffaloes and bovines at early gestation. Biocell. Mendoza: Instituto de Histología y Embriología, vol. 45, no. 2, p. 331-337. E-ISSN 1667-5746.0327-9545http://repositorio.unne.edu.ar/handle/123456789/53157Biocell, 2021, vol. 45, no. 2, p. 331-337.reponame:Repositorio Institucional de la Universidad Nacional del Nordeste (UNNE)instname:Universidad Nacional del Nordesteenginfo:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-nd/2.5/ar/Atribución-NoComercial-SinDerivadas 2.5 Argentina2025-10-16T10:06:23Zoai:repositorio.unne.edu.ar:123456789/53157instacron:UNNEInstitucionalhttp://repositorio.unne.edu.ar/Universidad públicaNo correspondehttp://repositorio.unne.edu.ar/oaiososa@bib.unne.edu.ar;sergio.alegria@unne.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:48712025-10-16 10:06:24.333Repositorio Institucional de la Universidad Nacional del Nordeste (UNNE) - Universidad Nacional del Nordestefalse
dc.title.none.fl_str_mv A comparative pattern of lectin-binding in the endometrial glands of the uterus and placenta of healthy buffaloes and bovines at early gestation
title A comparative pattern of lectin-binding in the endometrial glands of the uterus and placenta of healthy buffaloes and bovines at early gestation
spellingShingle A comparative pattern of lectin-binding in the endometrial glands of the uterus and placenta of healthy buffaloes and bovines at early gestation
Caspe, Sergio Gastón
Lectin-histochemistry
Water buffalo
Placenta
Glycosylation pattern
title_short A comparative pattern of lectin-binding in the endometrial glands of the uterus and placenta of healthy buffaloes and bovines at early gestation
title_full A comparative pattern of lectin-binding in the endometrial glands of the uterus and placenta of healthy buffaloes and bovines at early gestation
title_fullStr A comparative pattern of lectin-binding in the endometrial glands of the uterus and placenta of healthy buffaloes and bovines at early gestation
title_full_unstemmed A comparative pattern of lectin-binding in the endometrial glands of the uterus and placenta of healthy buffaloes and bovines at early gestation
title_sort A comparative pattern of lectin-binding in the endometrial glands of the uterus and placenta of healthy buffaloes and bovines at early gestation
dc.creator.none.fl_str_mv Caspe, Sergio Gastón
Konrad, José Luis
Moore, Dadin Prando
Sala, Juan Manuel
Lischinsky, Lilian
Campero, Carlos Manuel
Barbeito, Claudio Gustavo
author Caspe, Sergio Gastón
author_facet Caspe, Sergio Gastón
Konrad, José Luis
Moore, Dadin Prando
Sala, Juan Manuel
Lischinsky, Lilian
Campero, Carlos Manuel
Barbeito, Claudio Gustavo
author_role author
author2 Konrad, José Luis
Moore, Dadin Prando
Sala, Juan Manuel
Lischinsky, Lilian
Campero, Carlos Manuel
Barbeito, Claudio Gustavo
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv Lectin-histochemistry
Water buffalo
Placenta
Glycosylation pattern
topic Lectin-histochemistry
Water buffalo
Placenta
Glycosylation pattern
dc.description.none.fl_txt_mv Fil: Caspe, Sergio Gastón. Instituto Nacional de Tecnología Agropecuaria. Experimental Station Mercedes. Laboratory of Pathology; Argentina.
Fil: Konrad, José Luis. Universidad Nacional del Nordeste. Facultad de Ciencias Veterinarias; Argentina.
Fil: Moore, Dadin Prando. Instituto Nacional de Tecnología Agropecuaria. Experimental Station Balcarce. Animal Health Group; Argentina.
Fil: Sala, Juan Manuel. Instituto Nacional de Tecnología Agropecuaria. Experimental Station Mercedes. Laboratory of Pathology; Argentina.
Fil: Lischinsky, Lilian. Instituto Nacional de Tecnología Agropecuaria. Experimental Station Balcarce. Animal Health Group; Argentina.
Fil: Campero, Carlos Manuel. Instituto Nacional de Tecnología Agropecuaria. Experimental Station Balcarce. Animal Health Group; Argentina.
Fil: Barbeito, Claudio Gustavo. Universidad Nacional de La Plata. School of Veterinary Sciences. Laboratory of Histology and Embryology; Argentina.
Water buffalo (Bubalus bubalis) and domestic cattle (Bos taurus) are closely related species. However, embryo transfer interspecies has been attempted without any success. The failure in hybrid embryo-implantation is associated with the glycocode in the maternal-fetal interface. Glycosylation patterns have been studied in different species of ruminants; however, in B. bubalis, only the binucleated cells (BNC) have been analyzed. This glycocode is essential for a successful embryo-implantation and can be defined by Lectin-Histochemistry (LHC). The aim of this study is to compare the glycosylation pattern of placenta and uterus in water buffaloes and cattle by LHC. Tissue samples of placenta and uterus from pregnant Mediterranean female water buffaloes (Buf1) and Angus cows (Bov1) were analyzed. All animals were euthanized at 98 days of gestation. LHC was carried out using twelve lectins (Con A, LCA, PSA, sWGA, PHA-e, SBA, UEA-1, WGA, RCA-1, PNA, DBA, BSA-1). The intensity of lectin binding was semi- quantitatively scored using a scale of 0 (negative) to 3 (strongly positive). Difference between species was found in trophoblast layer by PSA, SWGA, PNA and BSA-1, in BNC, and in the mononuclear cells by LCA, PSA, PHA-e, DBA, BSA-1, PNA. In utero, differences in the apical cellular membrane and the secretion of glands were identified by DBA and RCA-1, and in the cytoplasm of those glandular epithelial cells by PHA-e, BSA-1, WGA, and SBA. In both species, BNC presented a strong positive reaction with DBA and SBA, a moderate response by LCA, PHA-e, BSA-1 and PNA lectin, and a low reaction by PSA, UEA-1, SWGA, WGA, Con A and RCA-1. The results found in this study suggest that although both species are closely related, glycosylation patterns in the placenta and uterus are different, thus providing a possible reason for embryo transfer not being possible between these species.
description Fil: Caspe, Sergio Gastón. Instituto Nacional de Tecnología Agropecuaria. Experimental Station Mercedes. Laboratory of Pathology; Argentina.
publishDate 2021
dc.date.none.fl_str_mv 2021
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv Caspe, Sergio Gastón, et al., 2021. A comparative pattern of lectin-binding in the endometrial glands of the uterus and placenta of healthy buffaloes and bovines at early gestation. Biocell. Mendoza: Instituto de Histología y Embriología, vol. 45, no. 2, p. 331-337. E-ISSN 1667-5746.
0327-9545
http://repositorio.unne.edu.ar/handle/123456789/53157
identifier_str_mv Caspe, Sergio Gastón, et al., 2021. A comparative pattern of lectin-binding in the endometrial glands of the uterus and placenta of healthy buffaloes and bovines at early gestation. Biocell. Mendoza: Instituto de Histología y Embriología, vol. 45, no. 2, p. 331-337. E-ISSN 1667-5746.
0327-9545
url http://repositorio.unne.edu.ar/handle/123456789/53157
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Atribución-NoComercial-SinDerivadas 2.5 Argentina
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Atribución-NoComercial-SinDerivadas 2.5 Argentina
dc.format.none.fl_str_mv application/pdf
p. 331-337
application/pdf
dc.publisher.none.fl_str_mv Instituto de Histología y Embriología
publisher.none.fl_str_mv Instituto de Histología y Embriología
dc.source.none.fl_str_mv Biocell, 2021, vol. 45, no. 2, p. 331-337.
reponame:Repositorio Institucional de la Universidad Nacional del Nordeste (UNNE)
instname:Universidad Nacional del Nordeste
reponame_str Repositorio Institucional de la Universidad Nacional del Nordeste (UNNE)
collection Repositorio Institucional de la Universidad Nacional del Nordeste (UNNE)
instname_str Universidad Nacional del Nordeste
repository.name.fl_str_mv Repositorio Institucional de la Universidad Nacional del Nordeste (UNNE) - Universidad Nacional del Nordeste
repository.mail.fl_str_mv ososa@bib.unne.edu.ar;sergio.alegria@unne.edu.ar
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