Biocontrol and Biostimulant Effect of a Fungal-Derived Extract in Commercial Varieties of Strawberry (Fragaria x ananassa Duch.)

Autores
Hael Conrad, Verónica; Diaz Ricci, Juan Carlos; Salazar, Sergio Miguel
Año de publicación
2022
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Objective: Strawberry postharvest fruits and plants are mainly affected by the fungus Botrytis cinerea (B. cinerea), which causes grey mould rot. Biological control agents and biostimulants can effectively control this fungus and promote plant growth. This study aimed to evaluate the capacity of a novel fungal-derived extract called culture filtrate (CF) to biocontrol grey mould rot in postharvest strawberries and plants while promoting plant growth.Methods: The CF extract was obtained from the liquid culture of a strawberry pathogenic fungus, gauzefiltered and inactivated by autoclave. The Fragaria x ananassa cultivars Camino Real, Benicia, and Merced were used. For the postharvest assay, fruit natural decay was evaluated by simulating real storage conditions. For the phytopathological assays, first-generation plants inoculated with B. cinerea were evaluated 26 days post infection. The effect of CF on plant growth and biomass promotion was evaluated 60 days post treatment (DPT).Results: The treatment with CF yielded healthier fruits of the three varieties, which presented minorseverity of grey mould symptoms compared to the controls treated with water or potato dextrose broth (PDB) by 7 DPT. For instance, 40% of Camino Real, 80% of Benicia, and 20% of Merced fruits presented an incipient lesion (severity 1), whereas no fruits treated with water or PDB showed this minor level of infection. On the contrary, around 50% and 70% of the control fruits presented the highest level of severity. Moreover, CF pre-treatment of Benicia “mother plants” protected first-generation untreated plants. Likewise, the best biostimulant effect was observed for Benicia plants, which presented an increased number of leaves and runner production 60 DPT.
Fil: Hael Conrad, Verónica. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Tucuman-Santiago del Estero. Estación Experimental Agropecuaria Famaillá; Argentina
Fil: Diaz Ricci, Juan Carlos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Instituto de Química Biológica; Argentina
Fil: Salazar, Sergio Miguel. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Tucuman-Santiago del Estero. Estación Experimental Agropecuaria Famaillá; Argentina. Universidad Nacional de Tucumán. Facultad de Agronomía y Zootecnia; Argentina
Materia
BIOLOGICAL CONTROL
BOTRYTIS CINEREA
STRAWBERRY
PHYTOVACCINES
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/228648
Acceder
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network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Biocontrol and Biostimulant Effect of a Fungal-Derived Extract in Commercial Varieties of Strawberry (Fragaria x ananassa Duch.)Hael Conrad, VerónicaDiaz Ricci, Juan CarlosSalazar, Sergio MiguelBIOLOGICAL CONTROLBOTRYTIS CINEREASTRAWBERRYPHYTOVACCINEShttps://purl.org/becyt/ford/4.4https://purl.org/becyt/ford/4Objective: Strawberry postharvest fruits and plants are mainly affected by the fungus Botrytis cinerea (B. cinerea), which causes grey mould rot. Biological control agents and biostimulants can effectively control this fungus and promote plant growth. This study aimed to evaluate the capacity of a novel fungal-derived extract called culture filtrate (CF) to biocontrol grey mould rot in postharvest strawberries and plants while promoting plant growth.Methods: The CF extract was obtained from the liquid culture of a strawberry pathogenic fungus, gauzefiltered and inactivated by autoclave. The Fragaria x ananassa cultivars Camino Real, Benicia, and Merced were used. For the postharvest assay, fruit natural decay was evaluated by simulating real storage conditions. For the phytopathological assays, first-generation plants inoculated with B. cinerea were evaluated 26 days post infection. The effect of CF on plant growth and biomass promotion was evaluated 60 days post treatment (DPT).Results: The treatment with CF yielded healthier fruits of the three varieties, which presented minorseverity of grey mould symptoms compared to the controls treated with water or potato dextrose broth (PDB) by 7 DPT. For instance, 40% of Camino Real, 80% of Benicia, and 20% of Merced fruits presented an incipient lesion (severity 1), whereas no fruits treated with water or PDB showed this minor level of infection. On the contrary, around 50% and 70% of the control fruits presented the highest level of severity. Moreover, CF pre-treatment of Benicia “mother plants” protected first-generation untreated plants. Likewise, the best biostimulant effect was observed for Benicia plants, which presented an increased number of leaves and runner production 60 DPT.Fil: Hael Conrad, Verónica. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Tucuman-Santiago del Estero. Estación Experimental Agropecuaria Famaillá; ArgentinaFil: Diaz Ricci, Juan Carlos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Instituto de Química Biológica; ArgentinaFil: Salazar, Sergio Miguel. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Tucuman-Santiago del Estero. Estación Experimental Agropecuaria Famaillá; Argentina. Universidad Nacional de Tucumán. Facultad de Agronomía y Zootecnia; ArgentinaInnovation Forever Publishing Group2022-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/228648Hael Conrad, Verónica; Diaz Ricci, Juan Carlos; Salazar, Sergio Miguel; Biocontrol and Biostimulant Effect of a Fungal-Derived Extract in Commercial Varieties of Strawberry (Fragaria x ananassa Duch.); Innovation Forever Publishing Group; Journal of Modern Agriculture and Biotechnology; 1(SI); 25; 12-2022; 1-122788-810XCONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.innovationforever.com/article.JMAB20220165info:eu-repo/semantics/altIdentifier/doi/10.53964/jmab.2022025info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-17T11:07:44Zoai:ri.conicet.gov.ar:11336/228648instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-17 11:07:44.477CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Biocontrol and Biostimulant Effect of a Fungal-Derived Extract in Commercial Varieties of Strawberry (Fragaria x ananassa Duch.)
title Biocontrol and Biostimulant Effect of a Fungal-Derived Extract in Commercial Varieties of Strawberry (Fragaria x ananassa Duch.)
spellingShingle Biocontrol and Biostimulant Effect of a Fungal-Derived Extract in Commercial Varieties of Strawberry (Fragaria x ananassa Duch.)
Hael Conrad, Verónica
BIOLOGICAL CONTROL
BOTRYTIS CINEREA
STRAWBERRY
PHYTOVACCINES
title_short Biocontrol and Biostimulant Effect of a Fungal-Derived Extract in Commercial Varieties of Strawberry (Fragaria x ananassa Duch.)
title_full Biocontrol and Biostimulant Effect of a Fungal-Derived Extract in Commercial Varieties of Strawberry (Fragaria x ananassa Duch.)
title_fullStr Biocontrol and Biostimulant Effect of a Fungal-Derived Extract in Commercial Varieties of Strawberry (Fragaria x ananassa Duch.)
title_full_unstemmed Biocontrol and Biostimulant Effect of a Fungal-Derived Extract in Commercial Varieties of Strawberry (Fragaria x ananassa Duch.)
title_sort Biocontrol and Biostimulant Effect of a Fungal-Derived Extract in Commercial Varieties of Strawberry (Fragaria x ananassa Duch.)
dc.creator.none.fl_str_mv Hael Conrad, Verónica
Diaz Ricci, Juan Carlos
Salazar, Sergio Miguel
author Hael Conrad, Verónica
author_facet Hael Conrad, Verónica
Diaz Ricci, Juan Carlos
Salazar, Sergio Miguel
author_role author
author2 Diaz Ricci, Juan Carlos
Salazar, Sergio Miguel
author2_role author
author
dc.subject.none.fl_str_mv BIOLOGICAL CONTROL
BOTRYTIS CINEREA
STRAWBERRY
PHYTOVACCINES
topic BIOLOGICAL CONTROL
BOTRYTIS CINEREA
STRAWBERRY
PHYTOVACCINES
purl_subject.fl_str_mv https://purl.org/becyt/ford/4.4
https://purl.org/becyt/ford/4
dc.description.none.fl_txt_mv Objective: Strawberry postharvest fruits and plants are mainly affected by the fungus Botrytis cinerea (B. cinerea), which causes grey mould rot. Biological control agents and biostimulants can effectively control this fungus and promote plant growth. This study aimed to evaluate the capacity of a novel fungal-derived extract called culture filtrate (CF) to biocontrol grey mould rot in postharvest strawberries and plants while promoting plant growth.Methods: The CF extract was obtained from the liquid culture of a strawberry pathogenic fungus, gauzefiltered and inactivated by autoclave. The Fragaria x ananassa cultivars Camino Real, Benicia, and Merced were used. For the postharvest assay, fruit natural decay was evaluated by simulating real storage conditions. For the phytopathological assays, first-generation plants inoculated with B. cinerea were evaluated 26 days post infection. The effect of CF on plant growth and biomass promotion was evaluated 60 days post treatment (DPT).Results: The treatment with CF yielded healthier fruits of the three varieties, which presented minorseverity of grey mould symptoms compared to the controls treated with water or potato dextrose broth (PDB) by 7 DPT. For instance, 40% of Camino Real, 80% of Benicia, and 20% of Merced fruits presented an incipient lesion (severity 1), whereas no fruits treated with water or PDB showed this minor level of infection. On the contrary, around 50% and 70% of the control fruits presented the highest level of severity. Moreover, CF pre-treatment of Benicia “mother plants” protected first-generation untreated plants. Likewise, the best biostimulant effect was observed for Benicia plants, which presented an increased number of leaves and runner production 60 DPT.
Fil: Hael Conrad, Verónica. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Tucuman-Santiago del Estero. Estación Experimental Agropecuaria Famaillá; Argentina
Fil: Diaz Ricci, Juan Carlos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Instituto de Química Biológica; Argentina
Fil: Salazar, Sergio Miguel. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Tucuman-Santiago del Estero. Estación Experimental Agropecuaria Famaillá; Argentina. Universidad Nacional de Tucumán. Facultad de Agronomía y Zootecnia; Argentina
description Objective: Strawberry postharvest fruits and plants are mainly affected by the fungus Botrytis cinerea (B. cinerea), which causes grey mould rot. Biological control agents and biostimulants can effectively control this fungus and promote plant growth. This study aimed to evaluate the capacity of a novel fungal-derived extract called culture filtrate (CF) to biocontrol grey mould rot in postharvest strawberries and plants while promoting plant growth.Methods: The CF extract was obtained from the liquid culture of a strawberry pathogenic fungus, gauzefiltered and inactivated by autoclave. The Fragaria x ananassa cultivars Camino Real, Benicia, and Merced were used. For the postharvest assay, fruit natural decay was evaluated by simulating real storage conditions. For the phytopathological assays, first-generation plants inoculated with B. cinerea were evaluated 26 days post infection. The effect of CF on plant growth and biomass promotion was evaluated 60 days post treatment (DPT).Results: The treatment with CF yielded healthier fruits of the three varieties, which presented minorseverity of grey mould symptoms compared to the controls treated with water or potato dextrose broth (PDB) by 7 DPT. For instance, 40% of Camino Real, 80% of Benicia, and 20% of Merced fruits presented an incipient lesion (severity 1), whereas no fruits treated with water or PDB showed this minor level of infection. On the contrary, around 50% and 70% of the control fruits presented the highest level of severity. Moreover, CF pre-treatment of Benicia “mother plants” protected first-generation untreated plants. Likewise, the best biostimulant effect was observed for Benicia plants, which presented an increased number of leaves and runner production 60 DPT.
publishDate 2022
dc.date.none.fl_str_mv 2022-12
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/228648
Hael Conrad, Verónica; Diaz Ricci, Juan Carlos; Salazar, Sergio Miguel; Biocontrol and Biostimulant Effect of a Fungal-Derived Extract in Commercial Varieties of Strawberry (Fragaria x ananassa Duch.); Innovation Forever Publishing Group; Journal of Modern Agriculture and Biotechnology; 1(SI); 25; 12-2022; 1-12
2788-810X
CONICET Digital
CONICET
url http://hdl.handle.net/11336/228648
identifier_str_mv Hael Conrad, Verónica; Diaz Ricci, Juan Carlos; Salazar, Sergio Miguel; Biocontrol and Biostimulant Effect of a Fungal-Derived Extract in Commercial Varieties of Strawberry (Fragaria x ananassa Duch.); Innovation Forever Publishing Group; Journal of Modern Agriculture and Biotechnology; 1(SI); 25; 12-2022; 1-12
2788-810X
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.innovationforever.com/article.JMAB20220165
info:eu-repo/semantics/altIdentifier/doi/10.53964/jmab.2022025
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Innovation Forever Publishing Group
publisher.none.fl_str_mv Innovation Forever Publishing Group
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.001348

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