Detection and selection of dye-degrading bacteria from surface waters with different degrees of contamination

Autores
Grifes Paisan, Luciana; Carballo, Rodrigo Andrés; Papalia, Mariana Andrea; Fortunato, María Susana; Radice, Marcela Alejandra; Korol, Sonia Edith; Gallego, Alfredo
Año de publicación
2025
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Biodegradability and resistance from indigenous bacterial communities to dyes were tested using samples from both polluted and unpolluted surface waters in Buenos Aires. Five dyes were selected for the study: Acid Black 210, Direct Orange 39, Malachite Green, Gentian Violet, and Alizarin Red. Water quality was assessed by measuring chemical oxygen demand, biochemical oxygen demand, and both Escherichia coli and enterococci counts. Biodegradability was tested using a respirometric method, while resistance was assessed by determining the minimum inhibitory concentration (MIC). No bacterial strains capable of degrading the dyes as the sole carbon source were isolated from the respirometric tests. However, from the MIC tests, 28 strains capable of dye discolouration were identified, using nutrient broth as a supplement. Two of them were able to degrade Malachite Green and Acid Black 210 at a concentration of 50 mg L 1 in less than 24 h and with an efficiency greater than 87%. These strains were identified as Aeromonas sp. and Shewanella sp. through MALDI-TOF/MS and 16S rRNA gene sequencing. The determination of biodegradability and resistance can be used to enhance the characterization of watercourses. Furthermore, this methodology provides a means to isolate biodegrading bacteria that could be applied in effluent treatment processes.
Fil: Grifes Paisan, Luciana. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina
Fil: Carballo, Rodrigo Andrés. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina
Fil: Papalia, Mariana Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Investigaciones en Bacteriología y Virología Molecular; Argentina
Fil: Fortunato, María Susana. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina
Fil: Radice, Marcela Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Investigaciones en Bacteriología y Virología Molecular; Argentina
Fil: Korol, Sonia Edith. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina
Fil: Gallego, Alfredo. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina
Materia
Acid Black 210
Malachite Green
Water pollution
Biodegradation
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/272292
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/272292
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Detection and selection of dye-degrading bacteria from surface waters with different degrees of contaminationGrifes Paisan, LucianaCarballo, Rodrigo AndrésPapalia, Mariana AndreaFortunato, María SusanaRadice, Marcela AlejandraKorol, Sonia EdithGallego, AlfredoAcid Black 210Malachite GreenWater pollutionBiodegradationhttps://purl.org/becyt/ford/1.5https://purl.org/becyt/ford/1Biodegradability and resistance from indigenous bacterial communities to dyes were tested using samples from both polluted and unpolluted surface waters in Buenos Aires. Five dyes were selected for the study: Acid Black 210, Direct Orange 39, Malachite Green, Gentian Violet, and Alizarin Red. Water quality was assessed by measuring chemical oxygen demand, biochemical oxygen demand, and both Escherichia coli and enterococci counts. Biodegradability was tested using a respirometric method, while resistance was assessed by determining the minimum inhibitory concentration (MIC). No bacterial strains capable of degrading the dyes as the sole carbon source were isolated from the respirometric tests. However, from the MIC tests, 28 strains capable of dye discolouration were identified, using nutrient broth as a supplement. Two of them were able to degrade Malachite Green and Acid Black 210 at a concentration of 50 mg L 1 in less than 24 h and with an efficiency greater than 87%. These strains were identified as Aeromonas sp. and Shewanella sp. through MALDI-TOF/MS and 16S rRNA gene sequencing. The determination of biodegradability and resistance can be used to enhance the characterization of watercourses. Furthermore, this methodology provides a means to isolate biodegrading bacteria that could be applied in effluent treatment processes.Fil: Grifes Paisan, Luciana. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; ArgentinaFil: Carballo, Rodrigo Andrés. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; ArgentinaFil: Papalia, Mariana Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Investigaciones en Bacteriología y Virología Molecular; ArgentinaFil: Fortunato, María Susana. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; ArgentinaFil: Radice, Marcela Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Investigaciones en Bacteriología y Virología Molecular; ArgentinaFil: Korol, Sonia Edith. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; ArgentinaFil: Gallego, Alfredo. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; ArgentinaIWA Publishing2025-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/272292Grifes Paisan, Luciana; Carballo, Rodrigo Andrés; Papalia, Mariana Andrea; Fortunato, María Susana; Radice, Marcela Alejandra; et al.; Detection and selection of dye-degrading bacteria from surface waters with different degrees of contamination; IWA Publishing; Journal of Water and Health; 23; 2; 1-2025; 140-1541477-8920CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://iwaponline.com/jwh/article/23/2/140/106731/Detection-and-selection-of-dye-degrading-bacteriainfo:eu-repo/semantics/altIdentifier/doi/10.2166/wh.2025.264info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-22T11:52:46Zoai:ri.conicet.gov.ar:11336/272292instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-22 11:52:46.776CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Detection and selection of dye-degrading bacteria from surface waters with different degrees of contamination
title Detection and selection of dye-degrading bacteria from surface waters with different degrees of contamination
spellingShingle Detection and selection of dye-degrading bacteria from surface waters with different degrees of contamination
Grifes Paisan, Luciana
Acid Black 210
Malachite Green
Water pollution
Biodegradation
title_short Detection and selection of dye-degrading bacteria from surface waters with different degrees of contamination
title_full Detection and selection of dye-degrading bacteria from surface waters with different degrees of contamination
title_fullStr Detection and selection of dye-degrading bacteria from surface waters with different degrees of contamination
title_full_unstemmed Detection and selection of dye-degrading bacteria from surface waters with different degrees of contamination
title_sort Detection and selection of dye-degrading bacteria from surface waters with different degrees of contamination
dc.creator.none.fl_str_mv Grifes Paisan, Luciana
Carballo, Rodrigo Andrés
Papalia, Mariana Andrea
Fortunato, María Susana
Radice, Marcela Alejandra
Korol, Sonia Edith
Gallego, Alfredo
author Grifes Paisan, Luciana
author_facet Grifes Paisan, Luciana
Carballo, Rodrigo Andrés
Papalia, Mariana Andrea
Fortunato, María Susana
Radice, Marcela Alejandra
Korol, Sonia Edith
Gallego, Alfredo
author_role author
author2 Carballo, Rodrigo Andrés
Papalia, Mariana Andrea
Fortunato, María Susana
Radice, Marcela Alejandra
Korol, Sonia Edith
Gallego, Alfredo
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv Acid Black 210
Malachite Green
Water pollution
Biodegradation
topic Acid Black 210
Malachite Green
Water pollution
Biodegradation
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.5
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Biodegradability and resistance from indigenous bacterial communities to dyes were tested using samples from both polluted and unpolluted surface waters in Buenos Aires. Five dyes were selected for the study: Acid Black 210, Direct Orange 39, Malachite Green, Gentian Violet, and Alizarin Red. Water quality was assessed by measuring chemical oxygen demand, biochemical oxygen demand, and both Escherichia coli and enterococci counts. Biodegradability was tested using a respirometric method, while resistance was assessed by determining the minimum inhibitory concentration (MIC). No bacterial strains capable of degrading the dyes as the sole carbon source were isolated from the respirometric tests. However, from the MIC tests, 28 strains capable of dye discolouration were identified, using nutrient broth as a supplement. Two of them were able to degrade Malachite Green and Acid Black 210 at a concentration of 50 mg L 1 in less than 24 h and with an efficiency greater than 87%. These strains were identified as Aeromonas sp. and Shewanella sp. through MALDI-TOF/MS and 16S rRNA gene sequencing. The determination of biodegradability and resistance can be used to enhance the characterization of watercourses. Furthermore, this methodology provides a means to isolate biodegrading bacteria that could be applied in effluent treatment processes.
Fil: Grifes Paisan, Luciana. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina
Fil: Carballo, Rodrigo Andrés. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina
Fil: Papalia, Mariana Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Investigaciones en Bacteriología y Virología Molecular; Argentina
Fil: Fortunato, María Susana. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina
Fil: Radice, Marcela Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Investigaciones en Bacteriología y Virología Molecular; Argentina
Fil: Korol, Sonia Edith. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina
Fil: Gallego, Alfredo. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina
description Biodegradability and resistance from indigenous bacterial communities to dyes were tested using samples from both polluted and unpolluted surface waters in Buenos Aires. Five dyes were selected for the study: Acid Black 210, Direct Orange 39, Malachite Green, Gentian Violet, and Alizarin Red. Water quality was assessed by measuring chemical oxygen demand, biochemical oxygen demand, and both Escherichia coli and enterococci counts. Biodegradability was tested using a respirometric method, while resistance was assessed by determining the minimum inhibitory concentration (MIC). No bacterial strains capable of degrading the dyes as the sole carbon source were isolated from the respirometric tests. However, from the MIC tests, 28 strains capable of dye discolouration were identified, using nutrient broth as a supplement. Two of them were able to degrade Malachite Green and Acid Black 210 at a concentration of 50 mg L 1 in less than 24 h and with an efficiency greater than 87%. These strains were identified as Aeromonas sp. and Shewanella sp. through MALDI-TOF/MS and 16S rRNA gene sequencing. The determination of biodegradability and resistance can be used to enhance the characterization of watercourses. Furthermore, this methodology provides a means to isolate biodegrading bacteria that could be applied in effluent treatment processes.
publishDate 2025
dc.date.none.fl_str_mv 2025-01
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/272292
Grifes Paisan, Luciana; Carballo, Rodrigo Andrés; Papalia, Mariana Andrea; Fortunato, María Susana; Radice, Marcela Alejandra; et al.; Detection and selection of dye-degrading bacteria from surface waters with different degrees of contamination; IWA Publishing; Journal of Water and Health; 23; 2; 1-2025; 140-154
1477-8920
CONICET Digital
CONICET
url http://hdl.handle.net/11336/272292
identifier_str_mv Grifes Paisan, Luciana; Carballo, Rodrigo Andrés; Papalia, Mariana Andrea; Fortunato, María Susana; Radice, Marcela Alejandra; et al.; Detection and selection of dye-degrading bacteria from surface waters with different degrees of contamination; IWA Publishing; Journal of Water and Health; 23; 2; 1-2025; 140-154
1477-8920
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://iwaponline.com/jwh/article/23/2/140/106731/Detection-and-selection-of-dye-degrading-bacteria
info:eu-repo/semantics/altIdentifier/doi/10.2166/wh.2025.264
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv IWA Publishing
publisher.none.fl_str_mv IWA Publishing
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 12.982451

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