Enhancement of laccase production and malachite green decolorization by co-culturing Ganoderma lucidum and Trametes versicolor in solid-state fermentation

Autores
Kuhar, José Francisco; Castiglia, Valeria Carolina; Levin, Laura Noemi
Año de publicación
2015
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Optimization of laccase production in white-rot fungi has been extensively studied. Metallic and aromatic compounds have been found to enhance enzyme production, but the development of bioremediation as an application field of this enzyme requires clean technologies. In this work, co-cultivation of Ganoderma lucidum and Trametes versicolor was performed, showing remarkable enhancement of laccase activity. Dual cultures were assayed for malachite green degradation (MG) in solid state fermentation (SSF) using a sawdust-based medium. The time for achieving complete decolorization of MG in co-cultivation was markedly shorter than that observed in monocultures. Dual-species treatment did not differ in wood dry weight loss and lignin, cellulose and hemicellulose degradation, compared to monocultures; but the selectivity index (lignin loss/cellulose loss) of dual cultures was markedly higher than those attained by monocultures. Moreover, a modified isoenzymatic laccase pattern was observed, showing one isoenzyme that was absent in monocultures. Dual cultures were able to decolorize and detoxify the dye more efficiently than the monocultures. The noticeable increase in laccase activity along with the more efficient decolorization and detoxification of MG by co-cultures of G. lucidum and T. versicolor in SSF makes this system a viable strategy for large scale application of white-rot cultures in bioremediation.
Fil: Kuhar, José Francisco. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Biodiversidad y Biología Experimental. Laboratorio de Micología Experimental; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Castiglia, Valeria Carolina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Biodiversidad y Biología Experimental. Laboratorio de Micología Experimental; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Levin, Laura Noemi. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Biodiversidad y Biología Experimental. Laboratorio de Micología Experimental; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Materia
Co-Cultivation
Malachite Green
Laccase
White-Rot Fungi
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/16133
Acceder
id CONICETDig_811ea355f158bdafe6abfea98a085964
oai_identifier_str oai:ri.conicet.gov.ar:11336/16133
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Enhancement of laccase production and malachite green decolorization by co-culturing Ganoderma lucidum and Trametes versicolor in solid-state fermentationKuhar, José FranciscoCastiglia, Valeria CarolinaLevin, Laura NoemiCo-CultivationMalachite GreenLaccaseWhite-Rot Fungihttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Optimization of laccase production in white-rot fungi has been extensively studied. Metallic and aromatic compounds have been found to enhance enzyme production, but the development of bioremediation as an application field of this enzyme requires clean technologies. In this work, co-cultivation of Ganoderma lucidum and Trametes versicolor was performed, showing remarkable enhancement of laccase activity. Dual cultures were assayed for malachite green degradation (MG) in solid state fermentation (SSF) using a sawdust-based medium. The time for achieving complete decolorization of MG in co-cultivation was markedly shorter than that observed in monocultures. Dual-species treatment did not differ in wood dry weight loss and lignin, cellulose and hemicellulose degradation, compared to monocultures; but the selectivity index (lignin loss/cellulose loss) of dual cultures was markedly higher than those attained by monocultures. Moreover, a modified isoenzymatic laccase pattern was observed, showing one isoenzyme that was absent in monocultures. Dual cultures were able to decolorize and detoxify the dye more efficiently than the monocultures. The noticeable increase in laccase activity along with the more efficient decolorization and detoxification of MG by co-cultures of G. lucidum and T. versicolor in SSF makes this system a viable strategy for large scale application of white-rot cultures in bioremediation.Fil: Kuhar, José Francisco. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Biodiversidad y Biología Experimental. Laboratorio de Micología Experimental; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Castiglia, Valeria Carolina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Biodiversidad y Biología Experimental. Laboratorio de Micología Experimental; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Levin, Laura Noemi. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Biodiversidad y Biología Experimental. Laboratorio de Micología Experimental; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaElsevier2015-10info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/16133Kuhar, José Francisco; Castiglia, Valeria Carolina; Levin, Laura Noemi; Enhancement of laccase production and malachite green decolorization by co-culturing Ganoderma lucidum and Trametes versicolor in solid-state fermentation; Elsevier; International Biodeterioration and Biodegradation; 104; 10-2015; 238-2430964-8305enginfo:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0964830515300378info:eu-repo/semantics/altIdentifier/doi/10.1016/j.ibiod.2015.06.017info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:46:08Zoai:ri.conicet.gov.ar:11336/16133instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:46:08.716CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Enhancement of laccase production and malachite green decolorization by co-culturing Ganoderma lucidum and Trametes versicolor in solid-state fermentation
title Enhancement of laccase production and malachite green decolorization by co-culturing Ganoderma lucidum and Trametes versicolor in solid-state fermentation
spellingShingle Enhancement of laccase production and malachite green decolorization by co-culturing Ganoderma lucidum and Trametes versicolor in solid-state fermentation
Kuhar, José Francisco
Co-Cultivation
Malachite Green
Laccase
White-Rot Fungi
title_short Enhancement of laccase production and malachite green decolorization by co-culturing Ganoderma lucidum and Trametes versicolor in solid-state fermentation
title_full Enhancement of laccase production and malachite green decolorization by co-culturing Ganoderma lucidum and Trametes versicolor in solid-state fermentation
title_fullStr Enhancement of laccase production and malachite green decolorization by co-culturing Ganoderma lucidum and Trametes versicolor in solid-state fermentation
title_full_unstemmed Enhancement of laccase production and malachite green decolorization by co-culturing Ganoderma lucidum and Trametes versicolor in solid-state fermentation
title_sort Enhancement of laccase production and malachite green decolorization by co-culturing Ganoderma lucidum and Trametes versicolor in solid-state fermentation
dc.creator.none.fl_str_mv Kuhar, José Francisco
Castiglia, Valeria Carolina
Levin, Laura Noemi
author Kuhar, José Francisco
author_facet Kuhar, José Francisco
Castiglia, Valeria Carolina
Levin, Laura Noemi
author_role author
author2 Castiglia, Valeria Carolina
Levin, Laura Noemi
author2_role author
author
dc.subject.none.fl_str_mv Co-Cultivation
Malachite Green
Laccase
White-Rot Fungi
topic Co-Cultivation
Malachite Green
Laccase
White-Rot Fungi
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Optimization of laccase production in white-rot fungi has been extensively studied. Metallic and aromatic compounds have been found to enhance enzyme production, but the development of bioremediation as an application field of this enzyme requires clean technologies. In this work, co-cultivation of Ganoderma lucidum and Trametes versicolor was performed, showing remarkable enhancement of laccase activity. Dual cultures were assayed for malachite green degradation (MG) in solid state fermentation (SSF) using a sawdust-based medium. The time for achieving complete decolorization of MG in co-cultivation was markedly shorter than that observed in monocultures. Dual-species treatment did not differ in wood dry weight loss and lignin, cellulose and hemicellulose degradation, compared to monocultures; but the selectivity index (lignin loss/cellulose loss) of dual cultures was markedly higher than those attained by monocultures. Moreover, a modified isoenzymatic laccase pattern was observed, showing one isoenzyme that was absent in monocultures. Dual cultures were able to decolorize and detoxify the dye more efficiently than the monocultures. The noticeable increase in laccase activity along with the more efficient decolorization and detoxification of MG by co-cultures of G. lucidum and T. versicolor in SSF makes this system a viable strategy for large scale application of white-rot cultures in bioremediation.
Fil: Kuhar, José Francisco. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Biodiversidad y Biología Experimental. Laboratorio de Micología Experimental; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Castiglia, Valeria Carolina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Biodiversidad y Biología Experimental. Laboratorio de Micología Experimental; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Levin, Laura Noemi. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Biodiversidad y Biología Experimental. Laboratorio de Micología Experimental; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
description Optimization of laccase production in white-rot fungi has been extensively studied. Metallic and aromatic compounds have been found to enhance enzyme production, but the development of bioremediation as an application field of this enzyme requires clean technologies. In this work, co-cultivation of Ganoderma lucidum and Trametes versicolor was performed, showing remarkable enhancement of laccase activity. Dual cultures were assayed for malachite green degradation (MG) in solid state fermentation (SSF) using a sawdust-based medium. The time for achieving complete decolorization of MG in co-cultivation was markedly shorter than that observed in monocultures. Dual-species treatment did not differ in wood dry weight loss and lignin, cellulose and hemicellulose degradation, compared to monocultures; but the selectivity index (lignin loss/cellulose loss) of dual cultures was markedly higher than those attained by monocultures. Moreover, a modified isoenzymatic laccase pattern was observed, showing one isoenzyme that was absent in monocultures. Dual cultures were able to decolorize and detoxify the dye more efficiently than the monocultures. The noticeable increase in laccase activity along with the more efficient decolorization and detoxification of MG by co-cultures of G. lucidum and T. versicolor in SSF makes this system a viable strategy for large scale application of white-rot cultures in bioremediation.
publishDate 2015
dc.date.none.fl_str_mv 2015-10
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/16133
Kuhar, José Francisco; Castiglia, Valeria Carolina; Levin, Laura Noemi; Enhancement of laccase production and malachite green decolorization by co-culturing Ganoderma lucidum and Trametes versicolor in solid-state fermentation; Elsevier; International Biodeterioration and Biodegradation; 104; 10-2015; 238-243
0964-8305
url http://hdl.handle.net/11336/16133
identifier_str_mv Kuhar, José Francisco; Castiglia, Valeria Carolina; Levin, Laura Noemi; Enhancement of laccase production and malachite green decolorization by co-culturing Ganoderma lucidum and Trametes versicolor in solid-state fermentation; Elsevier; International Biodeterioration and Biodegradation; 104; 10-2015; 238-243
0964-8305
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0964830515300378
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.ibiod.2015.06.017
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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