Angiotensin-(1-7) reduces norepinephrine release through a nitric oxide mechanism in rat hypothalamus

Autores
Gironacci, Mariela Mercedes; Vatta, Marcelo Sergio; Rodríguez Fermepin, Martin; Fernandez, Belisario Enrique; Peña, Clara
Año de publicación
2000
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Angiotensin (Ang)-(1-7) elicits a facilitatory presynaptic effect on peripheral noradrenergic neurotransmission, and because biological responses to the heptapeptide on occasion are tissue specific, the present investigation was undertaken to study its action on noradrenergic neurotransmission at the central level. In rat hypothalamus labeled with [3H]-norepinephrine, 100 to 600 nmol/L Ang-(1-7) diminished norepinephrine released by 25 mmol/L KCl. This effect was blocked by the selective angiotensin type 2 receptor antagonist PD 123319 (1 μmol/L) and by the specific Ang-(1-7) receptor antagonist [D-Ala7]Ang-(1-7) (1 μmol/L) but not by losartan (10 nmol/L to μmol/L), a selective angiotensin type 1 receptor antagonist. The inhibitory effect on noradrenergic neurotransmission caused by Ang-(1-7) was prevented by 10 μmol/L N(ω)-nitro-L-arginine methylester, an inhibitor of nitric oxide synthase activity, and was restored by 100 μmol/L L-arginine, precursor of nitric oxide synthesis. Methylene blue (10 μmol/L), an inhibitor of guanylate cyclase considered as the target of nitric oxide action, as well as Hoe 140 (10 μmol/L), a bradykinin B2- receptor antagonist, prevented the inhibitory effect of the heptapeptide on neuronal norepinephrine release, whereas no modification was observed in the presence of 0.1 to 10μmol/L indomethacin, a cyclooxygenase inhibitor. Our results indicate that Ang-(1-7) has a tissue-specific neuromodulatory effect on noradrenergic neurotransmission, being inhibitory at the central nervous system by a nitric oxide-dependent mechanism that involves angiotensin type 2 receptors and local bradykinin production.
Fil: Gironacci, Mariela Mercedes. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Físico-Química Biológicas "Prof. Alejandro C. Paladini". Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Química y Físico-Química Biológicas; Argentina
Fil: Vatta, Marcelo Sergio. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Metabolismo del Fármaco. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Química y Metabolismo del Fármaco; Argentina
Fil: Rodríguez Fermepin, Martin. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Ciencias Biológicas. Cátedra de Fisiopatología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina
Fil: Fernandez, Belisario Enrique. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Ciencias Biológicas. Cátedra de Fisiopatología; Argentina
Fil: Peña, Clara. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Físico-Química Biológicas "Prof. Alejandro C. Paladini". Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Química y Físico-Química Biológicas; Argentina
Materia
Angiotensin
Angiotensin Antagonist
Bradykinin
Nitric Oxide
Norepinephrine
Prostaglandins
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/40455
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/40455
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Angiotensin-(1-7) reduces norepinephrine release through a nitric oxide mechanism in rat hypothalamusGironacci, Mariela MercedesVatta, Marcelo SergioRodríguez Fermepin, MartinFernandez, Belisario EnriquePeña, ClaraAngiotensinAngiotensin AntagonistBradykininNitric OxideNorepinephrineProstaglandinshttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3Angiotensin (Ang)-(1-7) elicits a facilitatory presynaptic effect on peripheral noradrenergic neurotransmission, and because biological responses to the heptapeptide on occasion are tissue specific, the present investigation was undertaken to study its action on noradrenergic neurotransmission at the central level. In rat hypothalamus labeled with [3H]-norepinephrine, 100 to 600 nmol/L Ang-(1-7) diminished norepinephrine released by 25 mmol/L KCl. This effect was blocked by the selective angiotensin type 2 receptor antagonist PD 123319 (1 μmol/L) and by the specific Ang-(1-7) receptor antagonist [D-Ala7]Ang-(1-7) (1 μmol/L) but not by losartan (10 nmol/L to μmol/L), a selective angiotensin type 1 receptor antagonist. The inhibitory effect on noradrenergic neurotransmission caused by Ang-(1-7) was prevented by 10 μmol/L N(ω)-nitro-L-arginine methylester, an inhibitor of nitric oxide synthase activity, and was restored by 100 μmol/L L-arginine, precursor of nitric oxide synthesis. Methylene blue (10 μmol/L), an inhibitor of guanylate cyclase considered as the target of nitric oxide action, as well as Hoe 140 (10 μmol/L), a bradykinin B2- receptor antagonist, prevented the inhibitory effect of the heptapeptide on neuronal norepinephrine release, whereas no modification was observed in the presence of 0.1 to 10μmol/L indomethacin, a cyclooxygenase inhibitor. Our results indicate that Ang-(1-7) has a tissue-specific neuromodulatory effect on noradrenergic neurotransmission, being inhibitory at the central nervous system by a nitric oxide-dependent mechanism that involves angiotensin type 2 receptors and local bradykinin production.Fil: Gironacci, Mariela Mercedes. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Físico-Química Biológicas "Prof. Alejandro C. Paladini". Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Química y Físico-Química Biológicas; ArgentinaFil: Vatta, Marcelo Sergio. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Metabolismo del Fármaco. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Química y Metabolismo del Fármaco; ArgentinaFil: Rodríguez Fermepin, Martin. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Ciencias Biológicas. Cátedra de Fisiopatología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; ArgentinaFil: Fernandez, Belisario Enrique. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Ciencias Biológicas. Cátedra de Fisiopatología; ArgentinaFil: Peña, Clara. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Físico-Química Biológicas "Prof. Alejandro C. Paladini". Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Química y Físico-Química Biológicas; ArgentinaLippincott Williams2000-06info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/40455Gironacci, Mariela Mercedes; Vatta, Marcelo Sergio; Rodríguez Fermepin, Martin; Fernandez, Belisario Enrique; Peña, Clara; Angiotensin-(1-7) reduces norepinephrine release through a nitric oxide mechanism in rat hypothalamus; Lippincott Williams; Hypertension; 35; 6; 6-2000; 1248-12520194-911XCONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://hyper.ahajournals.org/content/35/6/1248.longinfo:eu-repo/semantics/altIdentifier/doi/10.1161/01.HYP.35.6.1248info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:46:01Zoai:ri.conicet.gov.ar:11336/40455instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:46:01.927CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Angiotensin-(1-7) reduces norepinephrine release through a nitric oxide mechanism in rat hypothalamus
title Angiotensin-(1-7) reduces norepinephrine release through a nitric oxide mechanism in rat hypothalamus
spellingShingle Angiotensin-(1-7) reduces norepinephrine release through a nitric oxide mechanism in rat hypothalamus
Gironacci, Mariela Mercedes
Angiotensin
Angiotensin Antagonist
Bradykinin
Nitric Oxide
Norepinephrine
Prostaglandins
title_short Angiotensin-(1-7) reduces norepinephrine release through a nitric oxide mechanism in rat hypothalamus
title_full Angiotensin-(1-7) reduces norepinephrine release through a nitric oxide mechanism in rat hypothalamus
title_fullStr Angiotensin-(1-7) reduces norepinephrine release through a nitric oxide mechanism in rat hypothalamus
title_full_unstemmed Angiotensin-(1-7) reduces norepinephrine release through a nitric oxide mechanism in rat hypothalamus
title_sort Angiotensin-(1-7) reduces norepinephrine release through a nitric oxide mechanism in rat hypothalamus
dc.creator.none.fl_str_mv Gironacci, Mariela Mercedes
Vatta, Marcelo Sergio
Rodríguez Fermepin, Martin
Fernandez, Belisario Enrique
Peña, Clara
author Gironacci, Mariela Mercedes
author_facet Gironacci, Mariela Mercedes
Vatta, Marcelo Sergio
Rodríguez Fermepin, Martin
Fernandez, Belisario Enrique
Peña, Clara
author_role author
author2 Vatta, Marcelo Sergio
Rodríguez Fermepin, Martin
Fernandez, Belisario Enrique
Peña, Clara
author2_role author
author
author
author
dc.subject.none.fl_str_mv Angiotensin
Angiotensin Antagonist
Bradykinin
Nitric Oxide
Norepinephrine
Prostaglandins
topic Angiotensin
Angiotensin Antagonist
Bradykinin
Nitric Oxide
Norepinephrine
Prostaglandins
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Angiotensin (Ang)-(1-7) elicits a facilitatory presynaptic effect on peripheral noradrenergic neurotransmission, and because biological responses to the heptapeptide on occasion are tissue specific, the present investigation was undertaken to study its action on noradrenergic neurotransmission at the central level. In rat hypothalamus labeled with [3H]-norepinephrine, 100 to 600 nmol/L Ang-(1-7) diminished norepinephrine released by 25 mmol/L KCl. This effect was blocked by the selective angiotensin type 2 receptor antagonist PD 123319 (1 μmol/L) and by the specific Ang-(1-7) receptor antagonist [D-Ala7]Ang-(1-7) (1 μmol/L) but not by losartan (10 nmol/L to μmol/L), a selective angiotensin type 1 receptor antagonist. The inhibitory effect on noradrenergic neurotransmission caused by Ang-(1-7) was prevented by 10 μmol/L N(ω)-nitro-L-arginine methylester, an inhibitor of nitric oxide synthase activity, and was restored by 100 μmol/L L-arginine, precursor of nitric oxide synthesis. Methylene blue (10 μmol/L), an inhibitor of guanylate cyclase considered as the target of nitric oxide action, as well as Hoe 140 (10 μmol/L), a bradykinin B2- receptor antagonist, prevented the inhibitory effect of the heptapeptide on neuronal norepinephrine release, whereas no modification was observed in the presence of 0.1 to 10μmol/L indomethacin, a cyclooxygenase inhibitor. Our results indicate that Ang-(1-7) has a tissue-specific neuromodulatory effect on noradrenergic neurotransmission, being inhibitory at the central nervous system by a nitric oxide-dependent mechanism that involves angiotensin type 2 receptors and local bradykinin production.
Fil: Gironacci, Mariela Mercedes. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Físico-Química Biológicas "Prof. Alejandro C. Paladini". Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Química y Físico-Química Biológicas; Argentina
Fil: Vatta, Marcelo Sergio. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Metabolismo del Fármaco. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Química y Metabolismo del Fármaco; Argentina
Fil: Rodríguez Fermepin, Martin. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Ciencias Biológicas. Cátedra de Fisiopatología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina
Fil: Fernandez, Belisario Enrique. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Ciencias Biológicas. Cátedra de Fisiopatología; Argentina
Fil: Peña, Clara. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Físico-Química Biológicas "Prof. Alejandro C. Paladini". Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Química y Físico-Química Biológicas; Argentina
description Angiotensin (Ang)-(1-7) elicits a facilitatory presynaptic effect on peripheral noradrenergic neurotransmission, and because biological responses to the heptapeptide on occasion are tissue specific, the present investigation was undertaken to study its action on noradrenergic neurotransmission at the central level. In rat hypothalamus labeled with [3H]-norepinephrine, 100 to 600 nmol/L Ang-(1-7) diminished norepinephrine released by 25 mmol/L KCl. This effect was blocked by the selective angiotensin type 2 receptor antagonist PD 123319 (1 μmol/L) and by the specific Ang-(1-7) receptor antagonist [D-Ala7]Ang-(1-7) (1 μmol/L) but not by losartan (10 nmol/L to μmol/L), a selective angiotensin type 1 receptor antagonist. The inhibitory effect on noradrenergic neurotransmission caused by Ang-(1-7) was prevented by 10 μmol/L N(ω)-nitro-L-arginine methylester, an inhibitor of nitric oxide synthase activity, and was restored by 100 μmol/L L-arginine, precursor of nitric oxide synthesis. Methylene blue (10 μmol/L), an inhibitor of guanylate cyclase considered as the target of nitric oxide action, as well as Hoe 140 (10 μmol/L), a bradykinin B2- receptor antagonist, prevented the inhibitory effect of the heptapeptide on neuronal norepinephrine release, whereas no modification was observed in the presence of 0.1 to 10μmol/L indomethacin, a cyclooxygenase inhibitor. Our results indicate that Ang-(1-7) has a tissue-specific neuromodulatory effect on noradrenergic neurotransmission, being inhibitory at the central nervous system by a nitric oxide-dependent mechanism that involves angiotensin type 2 receptors and local bradykinin production.
publishDate 2000
dc.date.none.fl_str_mv 2000-06
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/40455
Gironacci, Mariela Mercedes; Vatta, Marcelo Sergio; Rodríguez Fermepin, Martin; Fernandez, Belisario Enrique; Peña, Clara; Angiotensin-(1-7) reduces norepinephrine release through a nitric oxide mechanism in rat hypothalamus; Lippincott Williams; Hypertension; 35; 6; 6-2000; 1248-1252
0194-911X
CONICET Digital
CONICET
url http://hdl.handle.net/11336/40455
identifier_str_mv Gironacci, Mariela Mercedes; Vatta, Marcelo Sergio; Rodríguez Fermepin, Martin; Fernandez, Belisario Enrique; Peña, Clara; Angiotensin-(1-7) reduces norepinephrine release through a nitric oxide mechanism in rat hypothalamus; Lippincott Williams; Hypertension; 35; 6; 6-2000; 1248-1252
0194-911X
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://hyper.ahajournals.org/content/35/6/1248.long
info:eu-repo/semantics/altIdentifier/doi/10.1161/01.HYP.35.6.1248
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Lippincott Williams
publisher.none.fl_str_mv Lippincott Williams
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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