High expression of AID and active class switch recombination might account for a more aggressive disease in unmutated CLL patients: Link with an activated microenvironment in CLL d...

Autores
Palacios, Florencia; Moreno, Pilar; Morande, Pablo Elías; Abreu, Cecilia; Correa, Agustín; Porro, Valentina; Landoni, Ana Ines; Gabus, Raul; Giordano, Mirta Nilda; Dighiero, Guillermo; Pritsch, Otto; Oppezzo, Pablo
Año de publicación
2010
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Interaction of chronic lymphocytic leukemia (CLL) B cells with tissue microenvironment has been suggested to favor disease progression by promoting malignant B-cell growth. Previous work has shown expression in peripheral blood (PB) of CLL B cells of activation-induced cytidine deaminase (AID) among CLL patients with an unmutated (UM) profile of immunoglobulin genes and with ongoing class switch recombination (CSR) process. Because AID expression results from interaction with activated tissue microenvironment, we speculated whether the small subset with ongoing CSR is responsible for high levels of AID expression and could be derived from this particular microenvironment. In this work, we quantified AID expression and ongoing CSR in PB of 50 CLL patients and characterized the expression of different molecules related to microenvironment interaction. Our results show that among UM patients (1) high AID expression is restricted to the subpopulation of tumoral cells ongoing CSR; (2) this small subset expresses high levels of proliferation, antiapoptotic and progression markers (Ki-67, c-myc, Bcl-2, CD49d, and CCL3/4 chemokines). Overall, this work outlines the importance of a cellular subset in PB of UM CLL patients with a poor clinical outcome, high AID levels, and ongoing CSR, whose presence might be a hallmark of a recent contact with the microenvironment. © 2010 by The American Society of Hematology.
Fil: Palacios, Florencia. Instituto Pasteur de Montevideo; Uruguay
Fil: Moreno, Pilar. Instituto Pasteur de Montevideo; Uruguay
Fil: Morande, Pablo Elías. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Academia Nacional de Medicina de Buenos Aires. Instituto de Investigaciones Hematológicas "Mariano R. Castex". Departamento de Inmunología y Medicina Experimental; Argentina
Fil: Abreu, Cecilia. Instituto Pasteur de Montevideo; Uruguay
Fil: Correa, Agustín. Instituto Pasteur de Montevideo; Uruguay
Fil: Porro, Valentina. Instituto Pasteur de Montevideo; Uruguay
Fil: Landoni, Ana Ines. Hospital Maciel; Uruguay
Fil: Gabus, Raul. Hospital Maciel; Uruguay
Fil: Giordano, Mirta Nilda. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Academia Nacional de Medicina de Buenos Aires. Instituto de Investigaciones Hematológicas "Mariano R. Castex". Departamento de Inmunología y Medicina Experimental; Argentina
Fil: Dighiero, Guillermo. Instituto Pasteur de Montevideo; Uruguay
Fil: Pritsch, Otto. Instituto Pasteur de Montevideo; Uruguay
Fil: Oppezzo, Pablo. Instituto Pasteur de Montevideo; Uruguay
Materia
LEUKEMIA
AID
MICROENVIRONMENT
UNMUTATED
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/52947
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/52947
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling High expression of AID and active class switch recombination might account for a more aggressive disease in unmutated CLL patients: Link with an activated microenvironment in CLL diseasePalacios, FlorenciaMoreno, PilarMorande, Pablo ElíasAbreu, CeciliaCorrea, AgustínPorro, ValentinaLandoni, Ana InesGabus, RaulGiordano, Mirta NildaDighiero, GuillermoPritsch, OttoOppezzo, PabloLEUKEMIAAIDMICROENVIRONMENTUNMUTATEDhttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3Interaction of chronic lymphocytic leukemia (CLL) B cells with tissue microenvironment has been suggested to favor disease progression by promoting malignant B-cell growth. Previous work has shown expression in peripheral blood (PB) of CLL B cells of activation-induced cytidine deaminase (AID) among CLL patients with an unmutated (UM) profile of immunoglobulin genes and with ongoing class switch recombination (CSR) process. Because AID expression results from interaction with activated tissue microenvironment, we speculated whether the small subset with ongoing CSR is responsible for high levels of AID expression and could be derived from this particular microenvironment. In this work, we quantified AID expression and ongoing CSR in PB of 50 CLL patients and characterized the expression of different molecules related to microenvironment interaction. Our results show that among UM patients (1) high AID expression is restricted to the subpopulation of tumoral cells ongoing CSR; (2) this small subset expresses high levels of proliferation, antiapoptotic and progression markers (Ki-67, c-myc, Bcl-2, CD49d, and CCL3/4 chemokines). Overall, this work outlines the importance of a cellular subset in PB of UM CLL patients with a poor clinical outcome, high AID levels, and ongoing CSR, whose presence might be a hallmark of a recent contact with the microenvironment. © 2010 by The American Society of Hematology.Fil: Palacios, Florencia. Instituto Pasteur de Montevideo; UruguayFil: Moreno, Pilar. Instituto Pasteur de Montevideo; UruguayFil: Morande, Pablo Elías. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Academia Nacional de Medicina de Buenos Aires. Instituto de Investigaciones Hematológicas "Mariano R. Castex". Departamento de Inmunología y Medicina Experimental; ArgentinaFil: Abreu, Cecilia. Instituto Pasteur de Montevideo; UruguayFil: Correa, Agustín. Instituto Pasteur de Montevideo; UruguayFil: Porro, Valentina. Instituto Pasteur de Montevideo; UruguayFil: Landoni, Ana Ines. Hospital Maciel; UruguayFil: Gabus, Raul. Hospital Maciel; UruguayFil: Giordano, Mirta Nilda. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Academia Nacional de Medicina de Buenos Aires. Instituto de Investigaciones Hematológicas "Mariano R. Castex". Departamento de Inmunología y Medicina Experimental; ArgentinaFil: Dighiero, Guillermo. Instituto Pasteur de Montevideo; UruguayFil: Pritsch, Otto. Instituto Pasteur de Montevideo; UruguayFil: Oppezzo, Pablo. Instituto Pasteur de Montevideo; UruguayAmerican Society of Hematology2010-06info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/52947Palacios, Florencia; Moreno, Pilar; Morande, Pablo Elías; Abreu, Cecilia; Correa, Agustín; et al.; High expression of AID and active class switch recombination might account for a more aggressive disease in unmutated CLL patients: Link with an activated microenvironment in CLL disease; American Society of Hematology; Blood; 115; 22; 6-2010; 4488-44960006-4971CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1182/blood-2009-12-257758info:eu-repo/semantics/altIdentifier/url/.bloodjournal.org/content/115/22/4488info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:35:10Zoai:ri.conicet.gov.ar:11336/52947instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:35:10.396CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv High expression of AID and active class switch recombination might account for a more aggressive disease in unmutated CLL patients: Link with an activated microenvironment in CLL disease
title High expression of AID and active class switch recombination might account for a more aggressive disease in unmutated CLL patients: Link with an activated microenvironment in CLL disease
spellingShingle High expression of AID and active class switch recombination might account for a more aggressive disease in unmutated CLL patients: Link with an activated microenvironment in CLL disease
Palacios, Florencia
LEUKEMIA
AID
MICROENVIRONMENT
UNMUTATED
title_short High expression of AID and active class switch recombination might account for a more aggressive disease in unmutated CLL patients: Link with an activated microenvironment in CLL disease
title_full High expression of AID and active class switch recombination might account for a more aggressive disease in unmutated CLL patients: Link with an activated microenvironment in CLL disease
title_fullStr High expression of AID and active class switch recombination might account for a more aggressive disease in unmutated CLL patients: Link with an activated microenvironment in CLL disease
title_full_unstemmed High expression of AID and active class switch recombination might account for a more aggressive disease in unmutated CLL patients: Link with an activated microenvironment in CLL disease
title_sort High expression of AID and active class switch recombination might account for a more aggressive disease in unmutated CLL patients: Link with an activated microenvironment in CLL disease
dc.creator.none.fl_str_mv Palacios, Florencia
Moreno, Pilar
Morande, Pablo Elías
Abreu, Cecilia
Correa, Agustín
Porro, Valentina
Landoni, Ana Ines
Gabus, Raul
Giordano, Mirta Nilda
Dighiero, Guillermo
Pritsch, Otto
Oppezzo, Pablo
author Palacios, Florencia
author_facet Palacios, Florencia
Moreno, Pilar
Morande, Pablo Elías
Abreu, Cecilia
Correa, Agustín
Porro, Valentina
Landoni, Ana Ines
Gabus, Raul
Giordano, Mirta Nilda
Dighiero, Guillermo
Pritsch, Otto
Oppezzo, Pablo
author_role author
author2 Moreno, Pilar
Morande, Pablo Elías
Abreu, Cecilia
Correa, Agustín
Porro, Valentina
Landoni, Ana Ines
Gabus, Raul
Giordano, Mirta Nilda
Dighiero, Guillermo
Pritsch, Otto
Oppezzo, Pablo
author2_role author
author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv LEUKEMIA
AID
MICROENVIRONMENT
UNMUTATED
topic LEUKEMIA
AID
MICROENVIRONMENT
UNMUTATED
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Interaction of chronic lymphocytic leukemia (CLL) B cells with tissue microenvironment has been suggested to favor disease progression by promoting malignant B-cell growth. Previous work has shown expression in peripheral blood (PB) of CLL B cells of activation-induced cytidine deaminase (AID) among CLL patients with an unmutated (UM) profile of immunoglobulin genes and with ongoing class switch recombination (CSR) process. Because AID expression results from interaction with activated tissue microenvironment, we speculated whether the small subset with ongoing CSR is responsible for high levels of AID expression and could be derived from this particular microenvironment. In this work, we quantified AID expression and ongoing CSR in PB of 50 CLL patients and characterized the expression of different molecules related to microenvironment interaction. Our results show that among UM patients (1) high AID expression is restricted to the subpopulation of tumoral cells ongoing CSR; (2) this small subset expresses high levels of proliferation, antiapoptotic and progression markers (Ki-67, c-myc, Bcl-2, CD49d, and CCL3/4 chemokines). Overall, this work outlines the importance of a cellular subset in PB of UM CLL patients with a poor clinical outcome, high AID levels, and ongoing CSR, whose presence might be a hallmark of a recent contact with the microenvironment. © 2010 by The American Society of Hematology.
Fil: Palacios, Florencia. Instituto Pasteur de Montevideo; Uruguay
Fil: Moreno, Pilar. Instituto Pasteur de Montevideo; Uruguay
Fil: Morande, Pablo Elías. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Academia Nacional de Medicina de Buenos Aires. Instituto de Investigaciones Hematológicas "Mariano R. Castex". Departamento de Inmunología y Medicina Experimental; Argentina
Fil: Abreu, Cecilia. Instituto Pasteur de Montevideo; Uruguay
Fil: Correa, Agustín. Instituto Pasteur de Montevideo; Uruguay
Fil: Porro, Valentina. Instituto Pasteur de Montevideo; Uruguay
Fil: Landoni, Ana Ines. Hospital Maciel; Uruguay
Fil: Gabus, Raul. Hospital Maciel; Uruguay
Fil: Giordano, Mirta Nilda. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Academia Nacional de Medicina de Buenos Aires. Instituto de Investigaciones Hematológicas "Mariano R. Castex". Departamento de Inmunología y Medicina Experimental; Argentina
Fil: Dighiero, Guillermo. Instituto Pasteur de Montevideo; Uruguay
Fil: Pritsch, Otto. Instituto Pasteur de Montevideo; Uruguay
Fil: Oppezzo, Pablo. Instituto Pasteur de Montevideo; Uruguay
description Interaction of chronic lymphocytic leukemia (CLL) B cells with tissue microenvironment has been suggested to favor disease progression by promoting malignant B-cell growth. Previous work has shown expression in peripheral blood (PB) of CLL B cells of activation-induced cytidine deaminase (AID) among CLL patients with an unmutated (UM) profile of immunoglobulin genes and with ongoing class switch recombination (CSR) process. Because AID expression results from interaction with activated tissue microenvironment, we speculated whether the small subset with ongoing CSR is responsible for high levels of AID expression and could be derived from this particular microenvironment. In this work, we quantified AID expression and ongoing CSR in PB of 50 CLL patients and characterized the expression of different molecules related to microenvironment interaction. Our results show that among UM patients (1) high AID expression is restricted to the subpopulation of tumoral cells ongoing CSR; (2) this small subset expresses high levels of proliferation, antiapoptotic and progression markers (Ki-67, c-myc, Bcl-2, CD49d, and CCL3/4 chemokines). Overall, this work outlines the importance of a cellular subset in PB of UM CLL patients with a poor clinical outcome, high AID levels, and ongoing CSR, whose presence might be a hallmark of a recent contact with the microenvironment. © 2010 by The American Society of Hematology.
publishDate 2010
dc.date.none.fl_str_mv 2010-06
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/52947
Palacios, Florencia; Moreno, Pilar; Morande, Pablo Elías; Abreu, Cecilia; Correa, Agustín; et al.; High expression of AID and active class switch recombination might account for a more aggressive disease in unmutated CLL patients: Link with an activated microenvironment in CLL disease; American Society of Hematology; Blood; 115; 22; 6-2010; 4488-4496
0006-4971
CONICET Digital
CONICET
url http://hdl.handle.net/11336/52947
identifier_str_mv Palacios, Florencia; Moreno, Pilar; Morande, Pablo Elías; Abreu, Cecilia; Correa, Agustín; et al.; High expression of AID and active class switch recombination might account for a more aggressive disease in unmutated CLL patients: Link with an activated microenvironment in CLL disease; American Society of Hematology; Blood; 115; 22; 6-2010; 4488-4496
0006-4971
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1182/blood-2009-12-257758
info:eu-repo/semantics/altIdentifier/url/.bloodjournal.org/content/115/22/4488
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv American Society of Hematology
publisher.none.fl_str_mv American Society of Hematology
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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