Unraveling the genetic complexity of a cultivated breeding population of "yerba mate" (Ilex paraguariensis St. Hil.)
- Autores
- Paiva, Daniela Ivana; Cascales, Jimena; Rosetti, Maria Eva Natalia; Scherer, Rafael Alejandro; Gauchat, María Elena; Gottlieb, Alexandra Marina
- Año de publicación
- 2020
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- We report the first comprehensive multilocus molecular characterization of cultivated Ilex paraguariensis plants belonging to a breeding program. Using nuclear and homologous chloroplastidic molecular markers, we have genotyped 158 plants from four plantation sites. Analyses of the nuclear data (187 variable dominant loci) allowed detecting high diversity (0.569), the occurrence of four distinct genetic clusters, and a low but significant differentiation among sites. Additionally, 20 chloroplastidic alleles were identified applying five microsatellite polymorphic markers, and a high chloroplastidic diversity was recognized (0.505); two haplogroups were distinguished amongst the 63 haplotypes detected. Our results from both nuclear and plastidic markers indicate that most genetic variation reside within plantations sites (≥ 95%), and that these plantations were established on highly variable materials (either as seeds or plantlets) derived from, at least, 63 maternal lineages. Moreover, our study suggests that the genetic structure within each plantation site was most likely shaped by past admixture favored by farmers´ practices during the establishment of each plantation. Also, subsequent constraints in gene flow and/or a low level of shared polymorphism among plantations could have contributed to current structure.
Fil: Paiva, Daniela Ivana. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Misiones. Estación Experimental Agropecuaria Montecarlo; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Cascales, Jimena. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Ecología, Genética y Evolución de Buenos Aires. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Ecología, Genética y Evolución de Buenos Aires; Argentina
Fil: Rosetti, Maria Eva Natalia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Ecología, Genética y Evolución de Buenos Aires. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Ecología, Genética y Evolución de Buenos Aires; Argentina
Fil: Scherer, Rafael Alejandro. No especifíca;
Fil: Gauchat, María Elena. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Misiones. Estación Experimental Agropecuaria Montecarlo; Argentina
Fil: Gottlieb, Alexandra Marina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Ecología, Genética y Evolución de Buenos Aires. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Ecología, Genética y Evolución de Buenos Aires; Argentina - Materia
-
CHLOROPLASTIDIC MICROSATELLITES
GENETIC DIVERSITY
GENOTYPING
ISSR MARKERS - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by/2.5/ar/
- Repositorio
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/163610
Ver los metadatos del registro completo
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Unraveling the genetic complexity of a cultivated breeding population of "yerba mate" (Ilex paraguariensis St. Hil.)Paiva, Daniela IvanaCascales, JimenaRosetti, Maria Eva NataliaScherer, Rafael AlejandroGauchat, María ElenaGottlieb, Alexandra MarinaCHLOROPLASTIDIC MICROSATELLITESGENETIC DIVERSITYGENOTYPINGISSR MARKERShttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1We report the first comprehensive multilocus molecular characterization of cultivated Ilex paraguariensis plants belonging to a breeding program. Using nuclear and homologous chloroplastidic molecular markers, we have genotyped 158 plants from four plantation sites. Analyses of the nuclear data (187 variable dominant loci) allowed detecting high diversity (0.569), the occurrence of four distinct genetic clusters, and a low but significant differentiation among sites. Additionally, 20 chloroplastidic alleles were identified applying five microsatellite polymorphic markers, and a high chloroplastidic diversity was recognized (0.505); two haplogroups were distinguished amongst the 63 haplotypes detected. Our results from both nuclear and plastidic markers indicate that most genetic variation reside within plantations sites (≥ 95%), and that these plantations were established on highly variable materials (either as seeds or plantlets) derived from, at least, 63 maternal lineages. Moreover, our study suggests that the genetic structure within each plantation site was most likely shaped by past admixture favored by farmers´ practices during the establishment of each plantation. Also, subsequent constraints in gene flow and/or a low level of shared polymorphism among plantations could have contributed to current structure.Fil: Paiva, Daniela Ivana. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Misiones. Estación Experimental Agropecuaria Montecarlo; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Cascales, Jimena. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Ecología, Genética y Evolución de Buenos Aires. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Ecología, Genética y Evolución de Buenos Aires; ArgentinaFil: Rosetti, Maria Eva Natalia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Ecología, Genética y Evolución de Buenos Aires. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Ecología, Genética y Evolución de Buenos Aires; ArgentinaFil: Scherer, Rafael Alejandro. No especifíca;Fil: Gauchat, María Elena. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Misiones. Estación Experimental Agropecuaria Montecarlo; ArgentinaFil: Gottlieb, Alexandra Marina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Ecología, Genética y Evolución de Buenos Aires. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Ecología, Genética y Evolución de Buenos Aires; ArgentinaAcademia Brasileira de Ciencias2020-04info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/163610Paiva, Daniela Ivana; Cascales, Jimena; Rosetti, Maria Eva Natalia; Scherer, Rafael Alejandro; Gauchat, María Elena; et al.; Unraveling the genetic complexity of a cultivated breeding population of "yerba mate" (Ilex paraguariensis St. Hil.); Academia Brasileira de Ciencias; Anais da Academia Brasileira de Ciencias; 92; 1; 4-2020; 1-170001-37651678-2690CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1590/0001-3765202020190113info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:43:02Zoai:ri.conicet.gov.ar:11336/163610instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:43:03.105CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
dc.title.none.fl_str_mv |
Unraveling the genetic complexity of a cultivated breeding population of "yerba mate" (Ilex paraguariensis St. Hil.) |
title |
Unraveling the genetic complexity of a cultivated breeding population of "yerba mate" (Ilex paraguariensis St. Hil.) |
spellingShingle |
Unraveling the genetic complexity of a cultivated breeding population of "yerba mate" (Ilex paraguariensis St. Hil.) Paiva, Daniela Ivana CHLOROPLASTIDIC MICROSATELLITES GENETIC DIVERSITY GENOTYPING ISSR MARKERS |
title_short |
Unraveling the genetic complexity of a cultivated breeding population of "yerba mate" (Ilex paraguariensis St. Hil.) |
title_full |
Unraveling the genetic complexity of a cultivated breeding population of "yerba mate" (Ilex paraguariensis St. Hil.) |
title_fullStr |
Unraveling the genetic complexity of a cultivated breeding population of "yerba mate" (Ilex paraguariensis St. Hil.) |
title_full_unstemmed |
Unraveling the genetic complexity of a cultivated breeding population of "yerba mate" (Ilex paraguariensis St. Hil.) |
title_sort |
Unraveling the genetic complexity of a cultivated breeding population of "yerba mate" (Ilex paraguariensis St. Hil.) |
dc.creator.none.fl_str_mv |
Paiva, Daniela Ivana Cascales, Jimena Rosetti, Maria Eva Natalia Scherer, Rafael Alejandro Gauchat, María Elena Gottlieb, Alexandra Marina |
author |
Paiva, Daniela Ivana |
author_facet |
Paiva, Daniela Ivana Cascales, Jimena Rosetti, Maria Eva Natalia Scherer, Rafael Alejandro Gauchat, María Elena Gottlieb, Alexandra Marina |
author_role |
author |
author2 |
Cascales, Jimena Rosetti, Maria Eva Natalia Scherer, Rafael Alejandro Gauchat, María Elena Gottlieb, Alexandra Marina |
author2_role |
author author author author author |
dc.subject.none.fl_str_mv |
CHLOROPLASTIDIC MICROSATELLITES GENETIC DIVERSITY GENOTYPING ISSR MARKERS |
topic |
CHLOROPLASTIDIC MICROSATELLITES GENETIC DIVERSITY GENOTYPING ISSR MARKERS |
purl_subject.fl_str_mv |
https://purl.org/becyt/ford/1.6 https://purl.org/becyt/ford/1 |
dc.description.none.fl_txt_mv |
We report the first comprehensive multilocus molecular characterization of cultivated Ilex paraguariensis plants belonging to a breeding program. Using nuclear and homologous chloroplastidic molecular markers, we have genotyped 158 plants from four plantation sites. Analyses of the nuclear data (187 variable dominant loci) allowed detecting high diversity (0.569), the occurrence of four distinct genetic clusters, and a low but significant differentiation among sites. Additionally, 20 chloroplastidic alleles were identified applying five microsatellite polymorphic markers, and a high chloroplastidic diversity was recognized (0.505); two haplogroups were distinguished amongst the 63 haplotypes detected. Our results from both nuclear and plastidic markers indicate that most genetic variation reside within plantations sites (≥ 95%), and that these plantations were established on highly variable materials (either as seeds or plantlets) derived from, at least, 63 maternal lineages. Moreover, our study suggests that the genetic structure within each plantation site was most likely shaped by past admixture favored by farmers´ practices during the establishment of each plantation. Also, subsequent constraints in gene flow and/or a low level of shared polymorphism among plantations could have contributed to current structure. Fil: Paiva, Daniela Ivana. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Misiones. Estación Experimental Agropecuaria Montecarlo; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Cascales, Jimena. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Ecología, Genética y Evolución de Buenos Aires. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Ecología, Genética y Evolución de Buenos Aires; Argentina Fil: Rosetti, Maria Eva Natalia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Ecología, Genética y Evolución de Buenos Aires. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Ecología, Genética y Evolución de Buenos Aires; Argentina Fil: Scherer, Rafael Alejandro. No especifíca; Fil: Gauchat, María Elena. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Misiones. Estación Experimental Agropecuaria Montecarlo; Argentina Fil: Gottlieb, Alexandra Marina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Ecología, Genética y Evolución de Buenos Aires. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Ecología, Genética y Evolución de Buenos Aires; Argentina |
description |
We report the first comprehensive multilocus molecular characterization of cultivated Ilex paraguariensis plants belonging to a breeding program. Using nuclear and homologous chloroplastidic molecular markers, we have genotyped 158 plants from four plantation sites. Analyses of the nuclear data (187 variable dominant loci) allowed detecting high diversity (0.569), the occurrence of four distinct genetic clusters, and a low but significant differentiation among sites. Additionally, 20 chloroplastidic alleles were identified applying five microsatellite polymorphic markers, and a high chloroplastidic diversity was recognized (0.505); two haplogroups were distinguished amongst the 63 haplotypes detected. Our results from both nuclear and plastidic markers indicate that most genetic variation reside within plantations sites (≥ 95%), and that these plantations were established on highly variable materials (either as seeds or plantlets) derived from, at least, 63 maternal lineages. Moreover, our study suggests that the genetic structure within each plantation site was most likely shaped by past admixture favored by farmers´ practices during the establishment of each plantation. Also, subsequent constraints in gene flow and/or a low level of shared polymorphism among plantations could have contributed to current structure. |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020-04 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/163610 Paiva, Daniela Ivana; Cascales, Jimena; Rosetti, Maria Eva Natalia; Scherer, Rafael Alejandro; Gauchat, María Elena; et al.; Unraveling the genetic complexity of a cultivated breeding population of "yerba mate" (Ilex paraguariensis St. Hil.); Academia Brasileira de Ciencias; Anais da Academia Brasileira de Ciencias; 92; 1; 4-2020; 1-17 0001-3765 1678-2690 CONICET Digital CONICET |
url |
http://hdl.handle.net/11336/163610 |
identifier_str_mv |
Paiva, Daniela Ivana; Cascales, Jimena; Rosetti, Maria Eva Natalia; Scherer, Rafael Alejandro; Gauchat, María Elena; et al.; Unraveling the genetic complexity of a cultivated breeding population of "yerba mate" (Ilex paraguariensis St. Hil.); Academia Brasileira de Ciencias; Anais da Academia Brasileira de Ciencias; 92; 1; 4-2020; 1-17 0001-3765 1678-2690 CONICET Digital CONICET |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/doi/10.1590/0001-3765202020190113 |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by/2.5/ar/ |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by/2.5/ar/ |
dc.format.none.fl_str_mv |
application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
Academia Brasileira de Ciencias |
publisher.none.fl_str_mv |
Academia Brasileira de Ciencias |
dc.source.none.fl_str_mv |
reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas |
reponame_str |
CONICET Digital (CONICET) |
collection |
CONICET Digital (CONICET) |
instname_str |
Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.name.fl_str_mv |
CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.mail.fl_str_mv |
dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
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1844613354756243456 |
score |
13.070432 |