The small secreted effector protein MiSSP7.6 of Laccaria bicolor is required for the establishment of ectomycorrhizal symbiosis

Autores
Kang, Heng; Chen, Xin; Kemppainen, Minna Johanna; Pardo, Alejandro Guillermo; Veneault Fourrey, Claire; Kohler, Annegret; Martin, Francis M.
Año de publicación
2020
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
To establish and maintain a symbiotic relationship, the ectomycorrhizal fungus Laccaria bicolor releases mycorrhiza-induced small secreted proteins (MiSSPs) into host roots. Here, we have functionally characterized the MYCORRHIZA-iNDUCED SMALL SECRETED PROTEIN OF 7.6 kDa (MiSSP7.6) from L. bicolor by assessing its induced expression in ectomycorrhizae, silencing its expression by RNAi, and tracking in planta subcellular localization of its protein product. We also carried out yeast two-hybrid assays and bimolecular fluorescence complementation analysis to identify possible protein targets of the MiSSP7.6 effector in Populus roots. We showed that MiSSP7.6 expression is upregulated in ectomycorrhizal rootlets and associated extramatrical mycelium during the late stage of symbiosis development. RNAi mutants with a decreased MiSSP7.6 expression have a lower mycorrhization rate, suggesting a key role in the establishment of the symbiosis with plants. MiSSP7.6 is secreted, and it localizes both to the nuclei and cytoplasm in plant cells. MiSSP7.6 protein was shown to interact with two Populus Trihelix transcription factors. Furthermore, when coexpressed with one of the Trihelix transcription factors, MiSSP7.6 is localized to plant nuclei only. Our data suggest that MiSSP7.6 is a novel secreted symbiotic effector and is a potential determinant for ectomycorrhiza formation.
Fil: Kang, Heng. Institut National de la Recherche Agronomique; Francia
Fil: Chen, Xin. Institut National de la Recherche Agronomique; Francia
Fil: Kemppainen, Minna Johanna. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnologia. Instituto de Microbiologia Basica y Aplicada. Laboratorio de Micologia Molecular.; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Pardo, Alejandro Guillermo. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnologia. Instituto de Microbiologia Basica y Aplicada. Laboratorio de Micologia Molecular.; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Veneault Fourrey, Claire. Institut National de la Recherche Agronomique; Francia
Fil: Kohler, Annegret. Institut National de la Recherche Agronomique; Francia
Fil: Martin, Francis M.. Institut National de la Recherche Agronomique; Francia
Materia
ECTOMYCORRHIZAL FUNGUS
EFFECTOR
LACCARIA BICOLOR
SMALL-SECRETED PROTEIN
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/168462
Acceder
id CONICETDig_601774a3031041d431e116705d85e479
oai_identifier_str oai:ri.conicet.gov.ar:11336/168462
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling The small secreted effector protein MiSSP7.6 of Laccaria bicolor is required for the establishment of ectomycorrhizal symbiosisKang, HengChen, XinKemppainen, Minna JohannaPardo, Alejandro GuillermoVeneault Fourrey, ClaireKohler, AnnegretMartin, Francis M.ECTOMYCORRHIZAL FUNGUSEFFECTORLACCARIA BICOLORSMALL-SECRETED PROTEINhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1To establish and maintain a symbiotic relationship, the ectomycorrhizal fungus Laccaria bicolor releases mycorrhiza-induced small secreted proteins (MiSSPs) into host roots. Here, we have functionally characterized the MYCORRHIZA-iNDUCED SMALL SECRETED PROTEIN OF 7.6 kDa (MiSSP7.6) from L. bicolor by assessing its induced expression in ectomycorrhizae, silencing its expression by RNAi, and tracking in planta subcellular localization of its protein product. We also carried out yeast two-hybrid assays and bimolecular fluorescence complementation analysis to identify possible protein targets of the MiSSP7.6 effector in Populus roots. We showed that MiSSP7.6 expression is upregulated in ectomycorrhizal rootlets and associated extramatrical mycelium during the late stage of symbiosis development. RNAi mutants with a decreased MiSSP7.6 expression have a lower mycorrhization rate, suggesting a key role in the establishment of the symbiosis with plants. MiSSP7.6 is secreted, and it localizes both to the nuclei and cytoplasm in plant cells. MiSSP7.6 protein was shown to interact with two Populus Trihelix transcription factors. Furthermore, when coexpressed with one of the Trihelix transcription factors, MiSSP7.6 is localized to plant nuclei only. Our data suggest that MiSSP7.6 is a novel secreted symbiotic effector and is a potential determinant for ectomycorrhiza formation.Fil: Kang, Heng. Institut National de la Recherche Agronomique; FranciaFil: Chen, Xin. Institut National de la Recherche Agronomique; FranciaFil: Kemppainen, Minna Johanna. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnologia. Instituto de Microbiologia Basica y Aplicada. Laboratorio de Micologia Molecular.; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Pardo, Alejandro Guillermo. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnologia. Instituto de Microbiologia Basica y Aplicada. Laboratorio de Micologia Molecular.; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Veneault Fourrey, Claire. Institut National de la Recherche Agronomique; FranciaFil: Kohler, Annegret. Institut National de la Recherche Agronomique; FranciaFil: Martin, Francis M.. Institut National de la Recherche Agronomique; FranciaWiley Blackwell Publishing, Inc2020-04info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/168462Kang, Heng; Chen, Xin; Kemppainen, Minna Johanna; Pardo, Alejandro Guillermo; Veneault Fourrey, Claire; et al.; The small secreted effector protein MiSSP7.6 of Laccaria bicolor is required for the establishment of ectomycorrhizal symbiosis; Wiley Blackwell Publishing, Inc; Environmental Microbiology; 22; 4; 4-2020; 1435-14461462-2912CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/abs/10.1111/1462-2920.14959info:eu-repo/semantics/altIdentifier/doi/10.1111/1462-2920.14959info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T15:34:03Zoai:ri.conicet.gov.ar:11336/168462instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 15:34:03.715CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv The small secreted effector protein MiSSP7.6 of Laccaria bicolor is required for the establishment of ectomycorrhizal symbiosis
title The small secreted effector protein MiSSP7.6 of Laccaria bicolor is required for the establishment of ectomycorrhizal symbiosis
spellingShingle The small secreted effector protein MiSSP7.6 of Laccaria bicolor is required for the establishment of ectomycorrhizal symbiosis
Kang, Heng
ECTOMYCORRHIZAL FUNGUS
EFFECTOR
LACCARIA BICOLOR
SMALL-SECRETED PROTEIN
title_short The small secreted effector protein MiSSP7.6 of Laccaria bicolor is required for the establishment of ectomycorrhizal symbiosis
title_full The small secreted effector protein MiSSP7.6 of Laccaria bicolor is required for the establishment of ectomycorrhizal symbiosis
title_fullStr The small secreted effector protein MiSSP7.6 of Laccaria bicolor is required for the establishment of ectomycorrhizal symbiosis
title_full_unstemmed The small secreted effector protein MiSSP7.6 of Laccaria bicolor is required for the establishment of ectomycorrhizal symbiosis
title_sort The small secreted effector protein MiSSP7.6 of Laccaria bicolor is required for the establishment of ectomycorrhizal symbiosis
dc.creator.none.fl_str_mv Kang, Heng
Chen, Xin
Kemppainen, Minna Johanna
Pardo, Alejandro Guillermo
Veneault Fourrey, Claire
Kohler, Annegret
Martin, Francis M.
author Kang, Heng
author_facet Kang, Heng
Chen, Xin
Kemppainen, Minna Johanna
Pardo, Alejandro Guillermo
Veneault Fourrey, Claire
Kohler, Annegret
Martin, Francis M.
author_role author
author2 Chen, Xin
Kemppainen, Minna Johanna
Pardo, Alejandro Guillermo
Veneault Fourrey, Claire
Kohler, Annegret
Martin, Francis M.
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv ECTOMYCORRHIZAL FUNGUS
EFFECTOR
LACCARIA BICOLOR
SMALL-SECRETED PROTEIN
topic ECTOMYCORRHIZAL FUNGUS
EFFECTOR
LACCARIA BICOLOR
SMALL-SECRETED PROTEIN
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv To establish and maintain a symbiotic relationship, the ectomycorrhizal fungus Laccaria bicolor releases mycorrhiza-induced small secreted proteins (MiSSPs) into host roots. Here, we have functionally characterized the MYCORRHIZA-iNDUCED SMALL SECRETED PROTEIN OF 7.6 kDa (MiSSP7.6) from L. bicolor by assessing its induced expression in ectomycorrhizae, silencing its expression by RNAi, and tracking in planta subcellular localization of its protein product. We also carried out yeast two-hybrid assays and bimolecular fluorescence complementation analysis to identify possible protein targets of the MiSSP7.6 effector in Populus roots. We showed that MiSSP7.6 expression is upregulated in ectomycorrhizal rootlets and associated extramatrical mycelium during the late stage of symbiosis development. RNAi mutants with a decreased MiSSP7.6 expression have a lower mycorrhization rate, suggesting a key role in the establishment of the symbiosis with plants. MiSSP7.6 is secreted, and it localizes both to the nuclei and cytoplasm in plant cells. MiSSP7.6 protein was shown to interact with two Populus Trihelix transcription factors. Furthermore, when coexpressed with one of the Trihelix transcription factors, MiSSP7.6 is localized to plant nuclei only. Our data suggest that MiSSP7.6 is a novel secreted symbiotic effector and is a potential determinant for ectomycorrhiza formation.
Fil: Kang, Heng. Institut National de la Recherche Agronomique; Francia
Fil: Chen, Xin. Institut National de la Recherche Agronomique; Francia
Fil: Kemppainen, Minna Johanna. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnologia. Instituto de Microbiologia Basica y Aplicada. Laboratorio de Micologia Molecular.; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Pardo, Alejandro Guillermo. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnologia. Instituto de Microbiologia Basica y Aplicada. Laboratorio de Micologia Molecular.; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Veneault Fourrey, Claire. Institut National de la Recherche Agronomique; Francia
Fil: Kohler, Annegret. Institut National de la Recherche Agronomique; Francia
Fil: Martin, Francis M.. Institut National de la Recherche Agronomique; Francia
description To establish and maintain a symbiotic relationship, the ectomycorrhizal fungus Laccaria bicolor releases mycorrhiza-induced small secreted proteins (MiSSPs) into host roots. Here, we have functionally characterized the MYCORRHIZA-iNDUCED SMALL SECRETED PROTEIN OF 7.6 kDa (MiSSP7.6) from L. bicolor by assessing its induced expression in ectomycorrhizae, silencing its expression by RNAi, and tracking in planta subcellular localization of its protein product. We also carried out yeast two-hybrid assays and bimolecular fluorescence complementation analysis to identify possible protein targets of the MiSSP7.6 effector in Populus roots. We showed that MiSSP7.6 expression is upregulated in ectomycorrhizal rootlets and associated extramatrical mycelium during the late stage of symbiosis development. RNAi mutants with a decreased MiSSP7.6 expression have a lower mycorrhization rate, suggesting a key role in the establishment of the symbiosis with plants. MiSSP7.6 is secreted, and it localizes both to the nuclei and cytoplasm in plant cells. MiSSP7.6 protein was shown to interact with two Populus Trihelix transcription factors. Furthermore, when coexpressed with one of the Trihelix transcription factors, MiSSP7.6 is localized to plant nuclei only. Our data suggest that MiSSP7.6 is a novel secreted symbiotic effector and is a potential determinant for ectomycorrhiza formation.
publishDate 2020
dc.date.none.fl_str_mv 2020-04
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/168462
Kang, Heng; Chen, Xin; Kemppainen, Minna Johanna; Pardo, Alejandro Guillermo; Veneault Fourrey, Claire; et al.; The small secreted effector protein MiSSP7.6 of Laccaria bicolor is required for the establishment of ectomycorrhizal symbiosis; Wiley Blackwell Publishing, Inc; Environmental Microbiology; 22; 4; 4-2020; 1435-1446
1462-2912
CONICET Digital
CONICET
url http://hdl.handle.net/11336/168462
identifier_str_mv Kang, Heng; Chen, Xin; Kemppainen, Minna Johanna; Pardo, Alejandro Guillermo; Veneault Fourrey, Claire; et al.; The small secreted effector protein MiSSP7.6 of Laccaria bicolor is required for the establishment of ectomycorrhizal symbiosis; Wiley Blackwell Publishing, Inc; Environmental Microbiology; 22; 4; 4-2020; 1435-1446
1462-2912
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/abs/10.1111/1462-2920.14959
info:eu-repo/semantics/altIdentifier/doi/10.1111/1462-2920.14959
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Wiley Blackwell Publishing, Inc
publisher.none.fl_str_mv Wiley Blackwell Publishing, Inc
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.22299

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