Rodent models for the analysis of tissue clock function in metabolic rhythms research

• Autores: Tsang, Anthony H.; Astiz, Mariana; Leinweber, Brinja; Oster, Henrik
• Año de publicación: 2017
• Idioma: inglés
• Tipo de recurso: artículo
• Materia: BMAL1; CLOCK GENES; CONDITIONAL KNOCKOUT; CRE-LOXP SYSTEM; GENE TARGETING; METABOLISM
• Institución: Consejo Nacional de Investigaciones Científicas y Técnicas

The circadian timing system consists on a distributed network of cellular clocks that together coordinate 24-h rhythms of physiology and behavior. Clock function and metabolism are tightly coupled, from the cellular to the organismal level. Genetic and non-genetic approaches in rodents have been employed to study circadian clock function in the living organism. Due to the ubiquitous expression of clock genes and the intricate interaction between the circadian system and energy metabolism, genetic approaches targeting specific tissue clocks have been used to assess their contribution in systemic metabolic processes. However, special requirements regarding specificity and efficiency have to be met to allow for valid conclusions from such studies. In this review, we provide a brief summary of different approaches developed for dissecting tissue clock function in the metabolic context in rodents, compare their strengths and weaknesses, and suggest new strategies in assessing tissue clock output and the consequences of circadian clock disruption in vivo.
Fil: Tsang, Anthony H.. University of Lübeck; Alemania. University of Cambridge; Reino Unido
Fil: Astiz, Mariana. University of Lübeck; Alemania. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Leinweber, Brinja. University of Lübeck; Alemania
Fil: Oster, Henrik. University of Lübeck; Alemania