Behavioral phenotyping of Nestin-Cre mice: Implications for genetic mouse models of psychiatric disorders

Autores
Giusti, Sebastian Alejandro; Vercelli, Claudia Alejandra; Vogl, Annette M.; Kolarz, Adam W.; Pino, Natalia S.; Deussing, Jan M.; Refojo, Damian
Año de publicación
2014
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Genetic mouse models based on the Cre-loxP system have been extensively used to explore the influence of specific gene deletions on different aspects of behavioral neurobiology. However, the interpretation of the effects attributed to the gene deletion might be obscured by potential side effects secondary to the Cre recombinase transgene insertion or Cre activity, usually neither controlled nor reported. Here, we performed a comprehensive behavioral analysis of endophenotypes of neuropsychiatric disorders in the extensively used NestinCre mouse line, commonly employed to restrict genetic modifications to the CNS. We observed no alterations in locomotion, general exploratory activity, learning and memory, sociability, startle response and sensorimotor gating. Although the overall response to stimuli triggering anxiety-like behaviors remained unaltered in NestinCre mice, a strong impairment in the acquisition of both contextual- and cued-conditioned fear was observed. These results underline the importance of adequately controlling the behavioral performance of the employed Cre-lines per-se in pre-clinical neurobehavioral research.
Fil: Giusti, Sebastian Alejandro. Max Planck Institute of Psychiatry; Alemania
Fil: Vercelli, Claudia Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigación En Biomedicina de Buenos Aires; Argentina
Fil: Vogl, Annette M.. Max Planck Institute of Psychiatry; Alemania
Fil: Kolarz, Adam W.. Max Planck Institute of Psychiatry; Alemania
Fil: Pino, Natalia S.. Max Planck Institute of Psychiatry; Alemania
Fil: Deussing, Jan M.. Max Planck Institute of Psychiatry; Alemania
Fil: Refojo, Damian. Max Planck Institute of Psychiatry; Alemania
Materia
Cre-Loxp System
Nestin Cre
Mouse Model
Anxiety
Fear Conditioning
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/12313
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/12313
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Behavioral phenotyping of Nestin-Cre mice: Implications for genetic mouse models of psychiatric disordersGiusti, Sebastian AlejandroVercelli, Claudia AlejandraVogl, Annette M.Kolarz, Adam W.Pino, Natalia S.Deussing, Jan M.Refojo, DamianCre-Loxp SystemNestin CreMouse ModelAnxietyFear Conditioninghttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3Genetic mouse models based on the Cre-loxP system have been extensively used to explore the influence of specific gene deletions on different aspects of behavioral neurobiology. However, the interpretation of the effects attributed to the gene deletion might be obscured by potential side effects secondary to the Cre recombinase transgene insertion or Cre activity, usually neither controlled nor reported. Here, we performed a comprehensive behavioral analysis of endophenotypes of neuropsychiatric disorders in the extensively used NestinCre mouse line, commonly employed to restrict genetic modifications to the CNS. We observed no alterations in locomotion, general exploratory activity, learning and memory, sociability, startle response and sensorimotor gating. Although the overall response to stimuli triggering anxiety-like behaviors remained unaltered in NestinCre mice, a strong impairment in the acquisition of both contextual- and cued-conditioned fear was observed. These results underline the importance of adequately controlling the behavioral performance of the employed Cre-lines per-se in pre-clinical neurobehavioral research.Fil: Giusti, Sebastian Alejandro. Max Planck Institute of Psychiatry; AlemaniaFil: Vercelli, Claudia Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigación En Biomedicina de Buenos Aires; ArgentinaFil: Vogl, Annette M.. Max Planck Institute of Psychiatry; AlemaniaFil: Kolarz, Adam W.. Max Planck Institute of Psychiatry; AlemaniaFil: Pino, Natalia S.. Max Planck Institute of Psychiatry; AlemaniaFil: Deussing, Jan M.. Max Planck Institute of Psychiatry; AlemaniaFil: Refojo, Damian. Max Planck Institute of Psychiatry; AlemaniaElsevier2014-04info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/12313Giusti, Sebastian Alejandro; Vercelli, Claudia Alejandra; Vogl, Annette M.; Kolarz, Adam W.; Pino, Natalia S.; et al.; Behavioral phenotyping of Nestin-Cre mice: Implications for genetic mouse models of psychiatric disorders; Elsevier; Journal Of Psychiatric Research; 55; 4-2014; 87-950022-3956enginfo:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0022395614001083info:eu-repo/semantics/altIdentifier/doi/10.1016/j.jpsychires.2014.04.002info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:55:31Zoai:ri.conicet.gov.ar:11336/12313instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:55:32.079CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Behavioral phenotyping of Nestin-Cre mice: Implications for genetic mouse models of psychiatric disorders
title Behavioral phenotyping of Nestin-Cre mice: Implications for genetic mouse models of psychiatric disorders
spellingShingle Behavioral phenotyping of Nestin-Cre mice: Implications for genetic mouse models of psychiatric disorders
Giusti, Sebastian Alejandro
Cre-Loxp System
Nestin Cre
Mouse Model
Anxiety
Fear Conditioning
title_short Behavioral phenotyping of Nestin-Cre mice: Implications for genetic mouse models of psychiatric disorders
title_full Behavioral phenotyping of Nestin-Cre mice: Implications for genetic mouse models of psychiatric disorders
title_fullStr Behavioral phenotyping of Nestin-Cre mice: Implications for genetic mouse models of psychiatric disorders
title_full_unstemmed Behavioral phenotyping of Nestin-Cre mice: Implications for genetic mouse models of psychiatric disorders
title_sort Behavioral phenotyping of Nestin-Cre mice: Implications for genetic mouse models of psychiatric disorders
dc.creator.none.fl_str_mv Giusti, Sebastian Alejandro
Vercelli, Claudia Alejandra
Vogl, Annette M.
Kolarz, Adam W.
Pino, Natalia S.
Deussing, Jan M.
Refojo, Damian
author Giusti, Sebastian Alejandro
author_facet Giusti, Sebastian Alejandro
Vercelli, Claudia Alejandra
Vogl, Annette M.
Kolarz, Adam W.
Pino, Natalia S.
Deussing, Jan M.
Refojo, Damian
author_role author
author2 Vercelli, Claudia Alejandra
Vogl, Annette M.
Kolarz, Adam W.
Pino, Natalia S.
Deussing, Jan M.
Refojo, Damian
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv Cre-Loxp System
Nestin Cre
Mouse Model
Anxiety
Fear Conditioning
topic Cre-Loxp System
Nestin Cre
Mouse Model
Anxiety
Fear Conditioning
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Genetic mouse models based on the Cre-loxP system have been extensively used to explore the influence of specific gene deletions on different aspects of behavioral neurobiology. However, the interpretation of the effects attributed to the gene deletion might be obscured by potential side effects secondary to the Cre recombinase transgene insertion or Cre activity, usually neither controlled nor reported. Here, we performed a comprehensive behavioral analysis of endophenotypes of neuropsychiatric disorders in the extensively used NestinCre mouse line, commonly employed to restrict genetic modifications to the CNS. We observed no alterations in locomotion, general exploratory activity, learning and memory, sociability, startle response and sensorimotor gating. Although the overall response to stimuli triggering anxiety-like behaviors remained unaltered in NestinCre mice, a strong impairment in the acquisition of both contextual- and cued-conditioned fear was observed. These results underline the importance of adequately controlling the behavioral performance of the employed Cre-lines per-se in pre-clinical neurobehavioral research.
Fil: Giusti, Sebastian Alejandro. Max Planck Institute of Psychiatry; Alemania
Fil: Vercelli, Claudia Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigación En Biomedicina de Buenos Aires; Argentina
Fil: Vogl, Annette M.. Max Planck Institute of Psychiatry; Alemania
Fil: Kolarz, Adam W.. Max Planck Institute of Psychiatry; Alemania
Fil: Pino, Natalia S.. Max Planck Institute of Psychiatry; Alemania
Fil: Deussing, Jan M.. Max Planck Institute of Psychiatry; Alemania
Fil: Refojo, Damian. Max Planck Institute of Psychiatry; Alemania
description Genetic mouse models based on the Cre-loxP system have been extensively used to explore the influence of specific gene deletions on different aspects of behavioral neurobiology. However, the interpretation of the effects attributed to the gene deletion might be obscured by potential side effects secondary to the Cre recombinase transgene insertion or Cre activity, usually neither controlled nor reported. Here, we performed a comprehensive behavioral analysis of endophenotypes of neuropsychiatric disorders in the extensively used NestinCre mouse line, commonly employed to restrict genetic modifications to the CNS. We observed no alterations in locomotion, general exploratory activity, learning and memory, sociability, startle response and sensorimotor gating. Although the overall response to stimuli triggering anxiety-like behaviors remained unaltered in NestinCre mice, a strong impairment in the acquisition of both contextual- and cued-conditioned fear was observed. These results underline the importance of adequately controlling the behavioral performance of the employed Cre-lines per-se in pre-clinical neurobehavioral research.
publishDate 2014
dc.date.none.fl_str_mv 2014-04
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/12313
Giusti, Sebastian Alejandro; Vercelli, Claudia Alejandra; Vogl, Annette M.; Kolarz, Adam W.; Pino, Natalia S.; et al.; Behavioral phenotyping of Nestin-Cre mice: Implications for genetic mouse models of psychiatric disorders; Elsevier; Journal Of Psychiatric Research; 55; 4-2014; 87-95
0022-3956
url http://hdl.handle.net/11336/12313
identifier_str_mv Giusti, Sebastian Alejandro; Vercelli, Claudia Alejandra; Vogl, Annette M.; Kolarz, Adam W.; Pino, Natalia S.; et al.; Behavioral phenotyping of Nestin-Cre mice: Implications for genetic mouse models of psychiatric disorders; Elsevier; Journal Of Psychiatric Research; 55; 4-2014; 87-95
0022-3956
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0022395614001083
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.jpsychires.2014.04.002
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1844613674071752704
score 13.070432

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