Role of auxin during intercellular infection of Discaria trinervis by Frankia

Autores
Imanishi, Leandro Ezequiel; Perrine Walker, Francine; Ndour, Adama; Vayssières, Alice; Conejero, Geneviève; Lucas, Mikael; Champion, Anthony; Laplaze, Laurent; Wall, Luis Gabriel; Svistoonoff Sanchez, Sergio
Año de publicación
2014
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Nitrogen-fixing nodules induced by Frankia in the actinorhizal plant Discaria trinervis result from a primitive intercellular root invasion pathway that does not involve root hair deformation and infection threads. Here, we analyzed the role of auxin in this intercellular infection pathway at the molecular level and compared it with our previous work in the intracellular infected actinorhizal plant Casuarina glauca. Immunolocalisation experiments showed that auxin accumulated in Frankia-infected cells in both systems. We then characterized the expression of auxin transporters in D. trinervis nodules. No activation of the heterologous CgAUX1 promoter was detected in infected cells in D. trinervis. These results were confirmed with the endogenous D. trinervis gene, DtAUX1. However, DtAUX1 was expressed in the nodule meristem. Consistently, transgenic D. trinervis plants containing the auxin response marker DR5:VENUS showed expression of the reporter gene in the meristem. Immunolocalisation experiments using an antibody against the auxin efflux carrier PIN1, revealed the presence of this transporter in the plasma membrane of infected cells. Finally, we used in silico cellular models to analyse auxin fluxes in D. trinervis nodules. Our results point to the existence of divergent roles of auxin in intercellularly- and intracellularly-infected actinorhizal plants, an ancestral infection pathways leading to root nodule symbioses.
Fil: Imanishi, Leandro Ezequiel. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Centre National de la Recherche Scientifique. Institut de Recherche pour le Développement; Francia. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología; Argentina
Fil: Perrine Walker, Francine. Centre National de la Recherche Scientifique. Institut de Recherche pour le Développement; Francia
Fil: Ndour, Adama. Centre de Recherche de Bel Air, Laboratoire Commun de Microbiologie; Senegal
Fil: Vayssières, Alice. Centre National de la Recherche Scientifique. Institut de Recherche pour le Développement; Francia
Fil: Conejero, Geneviève. Centre de Recherche de Nantes. Institut National de la Recherche Agronomique; Francia
Fil: Lucas, Mikael. Centre National de la Recherche Scientifique. Institut de Recherche pour le Développement; Francia
Fil: Champion, Anthony. Centre de Recherche de Bel Air, Laboratoire Commun de Microbiologie; Senegal. Centre National de la Recherche Scientifique. Institut de Recherche pour le Développement; Francia
Fil: Laplaze, Laurent. Centre National de la Recherche Scientifique. Institut de Recherche pour le Développement; Francia. Centre de Recherche de Bel Air, Laboratoire Commun de Microbiologie; Senegal
Fil: Wall, Luis Gabriel. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología; Argentina
Fil: Svistoonoff Sanchez, Sergio. Centre de Recherche de Bel Air, Laboratoire Commun de Microbiologie; Senegal. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Centre National de la Recherche Scientifique. Institut de Recherche pour le Développement; Francia
Materia
AUXIN
ACTINORRHIZA
SYSTEMS BIOLOGY
ENDOSYMBIOSIS
MODEL
AUX1
PIN
NITROGEN FIXATION
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/103884
Acceder
id CONICETDig_9be436e539e1d3db86eca698c8deda70
oai_identifier_str oai:ri.conicet.gov.ar:11336/103884
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Role of auxin during intercellular infection of Discaria trinervis by FrankiaImanishi, Leandro EzequielPerrine Walker, FrancineNdour, AdamaVayssières, AliceConejero, GenevièveLucas, MikaelChampion, AnthonyLaplaze, LaurentWall, Luis GabrielSvistoonoff Sanchez, SergioAUXINACTINORRHIZASYSTEMS BIOLOGYENDOSYMBIOSISMODELAUX1PINNITROGEN FIXATIONhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Nitrogen-fixing nodules induced by Frankia in the actinorhizal plant Discaria trinervis result from a primitive intercellular root invasion pathway that does not involve root hair deformation and infection threads. Here, we analyzed the role of auxin in this intercellular infection pathway at the molecular level and compared it with our previous work in the intracellular infected actinorhizal plant Casuarina glauca. Immunolocalisation experiments showed that auxin accumulated in Frankia-infected cells in both systems. We then characterized the expression of auxin transporters in D. trinervis nodules. No activation of the heterologous CgAUX1 promoter was detected in infected cells in D. trinervis. These results were confirmed with the endogenous D. trinervis gene, DtAUX1. However, DtAUX1 was expressed in the nodule meristem. Consistently, transgenic D. trinervis plants containing the auxin response marker DR5:VENUS showed expression of the reporter gene in the meristem. Immunolocalisation experiments using an antibody against the auxin efflux carrier PIN1, revealed the presence of this transporter in the plasma membrane of infected cells. Finally, we used in silico cellular models to analyse auxin fluxes in D. trinervis nodules. Our results point to the existence of divergent roles of auxin in intercellularly- and intracellularly-infected actinorhizal plants, an ancestral infection pathways leading to root nodule symbioses.Fil: Imanishi, Leandro Ezequiel. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Centre National de la Recherche Scientifique. Institut de Recherche pour le Développement; Francia. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología; ArgentinaFil: Perrine Walker, Francine. Centre National de la Recherche Scientifique. Institut de Recherche pour le Développement; FranciaFil: Ndour, Adama. Centre de Recherche de Bel Air, Laboratoire Commun de Microbiologie; SenegalFil: Vayssières, Alice. Centre National de la Recherche Scientifique. Institut de Recherche pour le Développement; FranciaFil: Conejero, Geneviève. Centre de Recherche de Nantes. Institut National de la Recherche Agronomique; FranciaFil: Lucas, Mikael. Centre National de la Recherche Scientifique. Institut de Recherche pour le Développement; FranciaFil: Champion, Anthony. Centre de Recherche de Bel Air, Laboratoire Commun de Microbiologie; Senegal. Centre National de la Recherche Scientifique. Institut de Recherche pour le Développement; FranciaFil: Laplaze, Laurent. Centre National de la Recherche Scientifique. Institut de Recherche pour le Développement; Francia. Centre de Recherche de Bel Air, Laboratoire Commun de Microbiologie; SenegalFil: Wall, Luis Gabriel. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología; ArgentinaFil: Svistoonoff Sanchez, Sergio. Centre de Recherche de Bel Air, Laboratoire Commun de Microbiologie; Senegal. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Centre National de la Recherche Scientifique. Institut de Recherche pour le Développement; FranciaFrontiers in Plant Science2014-08info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/103884Imanishi, Leandro Ezequiel; Perrine Walker, Francine; Ndour, Adama; Vayssières, Alice; Conejero, Geneviève; et al.; Role of auxin during intercellular infection of Discaria trinervis by Frankia; Frontiers in Plant Science; Frontiers in Plant Science; 5; 399; 8-2014; 1-91664-462XCONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.3389/fpls.2014.00399info:eu-repo/semantics/altIdentifier/url/https://www.frontiersin.org/articles/10.3389/fpls.2014.00399/fullinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-11-12T09:42:14Zoai:ri.conicet.gov.ar:11336/103884instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-11-12 09:42:14.596CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Role of auxin during intercellular infection of Discaria trinervis by Frankia
title Role of auxin during intercellular infection of Discaria trinervis by Frankia
spellingShingle Role of auxin during intercellular infection of Discaria trinervis by Frankia
Imanishi, Leandro Ezequiel
AUXIN
ACTINORRHIZA
SYSTEMS BIOLOGY
ENDOSYMBIOSIS
MODEL
AUX1
PIN
NITROGEN FIXATION
title_short Role of auxin during intercellular infection of Discaria trinervis by Frankia
title_full Role of auxin during intercellular infection of Discaria trinervis by Frankia
title_fullStr Role of auxin during intercellular infection of Discaria trinervis by Frankia
title_full_unstemmed Role of auxin during intercellular infection of Discaria trinervis by Frankia
title_sort Role of auxin during intercellular infection of Discaria trinervis by Frankia
dc.creator.none.fl_str_mv Imanishi, Leandro Ezequiel
Perrine Walker, Francine
Ndour, Adama
Vayssières, Alice
Conejero, Geneviève
Lucas, Mikael
Champion, Anthony
Laplaze, Laurent
Wall, Luis Gabriel
Svistoonoff Sanchez, Sergio
author Imanishi, Leandro Ezequiel
author_facet Imanishi, Leandro Ezequiel
Perrine Walker, Francine
Ndour, Adama
Vayssières, Alice
Conejero, Geneviève
Lucas, Mikael
Champion, Anthony
Laplaze, Laurent
Wall, Luis Gabriel
Svistoonoff Sanchez, Sergio
author_role author
author2 Perrine Walker, Francine
Ndour, Adama
Vayssières, Alice
Conejero, Geneviève
Lucas, Mikael
Champion, Anthony
Laplaze, Laurent
Wall, Luis Gabriel
Svistoonoff Sanchez, Sergio
author2_role author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv AUXIN
ACTINORRHIZA
SYSTEMS BIOLOGY
ENDOSYMBIOSIS
MODEL
AUX1
PIN
NITROGEN FIXATION
topic AUXIN
ACTINORRHIZA
SYSTEMS BIOLOGY
ENDOSYMBIOSIS
MODEL
AUX1
PIN
NITROGEN FIXATION
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Nitrogen-fixing nodules induced by Frankia in the actinorhizal plant Discaria trinervis result from a primitive intercellular root invasion pathway that does not involve root hair deformation and infection threads. Here, we analyzed the role of auxin in this intercellular infection pathway at the molecular level and compared it with our previous work in the intracellular infected actinorhizal plant Casuarina glauca. Immunolocalisation experiments showed that auxin accumulated in Frankia-infected cells in both systems. We then characterized the expression of auxin transporters in D. trinervis nodules. No activation of the heterologous CgAUX1 promoter was detected in infected cells in D. trinervis. These results were confirmed with the endogenous D. trinervis gene, DtAUX1. However, DtAUX1 was expressed in the nodule meristem. Consistently, transgenic D. trinervis plants containing the auxin response marker DR5:VENUS showed expression of the reporter gene in the meristem. Immunolocalisation experiments using an antibody against the auxin efflux carrier PIN1, revealed the presence of this transporter in the plasma membrane of infected cells. Finally, we used in silico cellular models to analyse auxin fluxes in D. trinervis nodules. Our results point to the existence of divergent roles of auxin in intercellularly- and intracellularly-infected actinorhizal plants, an ancestral infection pathways leading to root nodule symbioses.
Fil: Imanishi, Leandro Ezequiel. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Centre National de la Recherche Scientifique. Institut de Recherche pour le Développement; Francia. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología; Argentina
Fil: Perrine Walker, Francine. Centre National de la Recherche Scientifique. Institut de Recherche pour le Développement; Francia
Fil: Ndour, Adama. Centre de Recherche de Bel Air, Laboratoire Commun de Microbiologie; Senegal
Fil: Vayssières, Alice. Centre National de la Recherche Scientifique. Institut de Recherche pour le Développement; Francia
Fil: Conejero, Geneviève. Centre de Recherche de Nantes. Institut National de la Recherche Agronomique; Francia
Fil: Lucas, Mikael. Centre National de la Recherche Scientifique. Institut de Recherche pour le Développement; Francia
Fil: Champion, Anthony. Centre de Recherche de Bel Air, Laboratoire Commun de Microbiologie; Senegal. Centre National de la Recherche Scientifique. Institut de Recherche pour le Développement; Francia
Fil: Laplaze, Laurent. Centre National de la Recherche Scientifique. Institut de Recherche pour le Développement; Francia. Centre de Recherche de Bel Air, Laboratoire Commun de Microbiologie; Senegal
Fil: Wall, Luis Gabriel. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología; Argentina
Fil: Svistoonoff Sanchez, Sergio. Centre de Recherche de Bel Air, Laboratoire Commun de Microbiologie; Senegal. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Centre National de la Recherche Scientifique. Institut de Recherche pour le Développement; Francia
description Nitrogen-fixing nodules induced by Frankia in the actinorhizal plant Discaria trinervis result from a primitive intercellular root invasion pathway that does not involve root hair deformation and infection threads. Here, we analyzed the role of auxin in this intercellular infection pathway at the molecular level and compared it with our previous work in the intracellular infected actinorhizal plant Casuarina glauca. Immunolocalisation experiments showed that auxin accumulated in Frankia-infected cells in both systems. We then characterized the expression of auxin transporters in D. trinervis nodules. No activation of the heterologous CgAUX1 promoter was detected in infected cells in D. trinervis. These results were confirmed with the endogenous D. trinervis gene, DtAUX1. However, DtAUX1 was expressed in the nodule meristem. Consistently, transgenic D. trinervis plants containing the auxin response marker DR5:VENUS showed expression of the reporter gene in the meristem. Immunolocalisation experiments using an antibody against the auxin efflux carrier PIN1, revealed the presence of this transporter in the plasma membrane of infected cells. Finally, we used in silico cellular models to analyse auxin fluxes in D. trinervis nodules. Our results point to the existence of divergent roles of auxin in intercellularly- and intracellularly-infected actinorhizal plants, an ancestral infection pathways leading to root nodule symbioses.
publishDate 2014
dc.date.none.fl_str_mv 2014-08
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/103884
Imanishi, Leandro Ezequiel; Perrine Walker, Francine; Ndour, Adama; Vayssières, Alice; Conejero, Geneviève; et al.; Role of auxin during intercellular infection of Discaria trinervis by Frankia; Frontiers in Plant Science; Frontiers in Plant Science; 5; 399; 8-2014; 1-9
1664-462X
CONICET Digital
CONICET
url http://hdl.handle.net/11336/103884
identifier_str_mv Imanishi, Leandro Ezequiel; Perrine Walker, Francine; Ndour, Adama; Vayssières, Alice; Conejero, Geneviève; et al.; Role of auxin during intercellular infection of Discaria trinervis by Frankia; Frontiers in Plant Science; Frontiers in Plant Science; 5; 399; 8-2014; 1-9
1664-462X
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.3389/fpls.2014.00399
info:eu-repo/semantics/altIdentifier/url/https://www.frontiersin.org/articles/10.3389/fpls.2014.00399/full
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Frontiers in Plant Science
publisher.none.fl_str_mv Frontiers in Plant Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.223955

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