Cholinoceptor Modulation on Nitric Oxide Regulates Prostaglandin E2 and Metalloproteinase-3 Production in Experimentally Induced Inflammation of Rat Dental Pulp

Autores
de Couto Pita, Alejandra Karina; Borda, Enri Santiago; Ganzinelli, Sabrina Belen; Passafaro, Daniela; Sterin, Leonor Josefina
Año de publicación
2009
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The purpose of this study was to investigate the role of muscarinic acetylcholine receptor (mAChR) activity in the regulation of inducible nitric oxide synthase (iNOS) activity, prostaglandin E2 (PGE2), and metalloproteinase-3 (MMP-3) in experimentally induced inflammation of rat incisors dental pulp. Inflammation was induced by application of bacterial lipopolysaccharide (LPS) to the pulp. Extirpated pulp tissue samples were incubated in saline solution until the various experiments were performed. Saline-treated pulp and healthy pulp were used as controls. NOS activity was measured by the production of [U-14C]-citrulline from [U-14C]-arginine. PGE2 and MMP-3 production were evaluated by an enzyme-linked immunosorbent assay (ELISA) and cyclooxygenase (cox-1 and cox-2) messenger RNA levels were measured using a reverse-transcriptase polymerase chain reaction by coamplification of target complementary DNA with a single set of primers. The application of LPS to the pulp increased NOS activity, PGE2, and MMP-3 production associated with iNOS overactivity. Moreover, PGE2 and MMP-3 production were the result of cox-2 expression. Pilocarpine (5 × 10-11 mol/L to 5 × 10-9 mol/L), acting on mAChRs, triggered a negative effect on NOS activity, PGE2, and MMP-3 production. In control pulp, no action of pilocarpine was observed. Pulpitis changed mAChR conformation, increasing its coupling efficiency to transducing molecules that in turn activate iNOS. The capacity of pilocarpine to prevent iNOS activity, PGE2, and MMP-3 by acting on mAChR mutation induced by pulpitis might be useful therapeutically as a local treatment.
Fil: de Couto Pita, Alejandra Karina. Universidad de Buenos Aires. Facultad de Odontología. Cátedra de Farmacología; Argentina
Fil: Borda, Enri Santiago. Universidad de Buenos Aires. Facultad de Odontología. Cátedra de Farmacología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina
Fil: Ganzinelli, Sabrina Belen. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina. Universidad de Buenos Aires. Facultad de Odontología. Cátedra de Farmacología; Argentina
Fil: Passafaro, Daniela. Universidad de Buenos Aires. Facultad de Odontología. Cátedra de Farmacología; Argentina
Fil: Sterin, Leonor Josefina. Universidad de Buenos Aires. Facultad de Odontología. Cátedra de Farmacología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina
Materia
CYCLOOXYGENASE-1
CYCLOOXYGENASE-2
DENTAL PULP
METALLOPROTEINASE-3
MUSCARINIC ACETYLCHOLINE RECEPTOR
NITRIC OXIDE SYNTHASE
PILOCARPINE
PROSTAGLANDIN E2
PULPITIS
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/114224
Acceder
id CONICETDig_5f396f109854901772dbc1791f73d3f2
oai_identifier_str oai:ri.conicet.gov.ar:11336/114224
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Cholinoceptor Modulation on Nitric Oxide Regulates Prostaglandin E2 and Metalloproteinase-3 Production in Experimentally Induced Inflammation of Rat Dental Pulpde Couto Pita, Alejandra KarinaBorda, Enri SantiagoGanzinelli, Sabrina BelenPassafaro, DanielaSterin, Leonor JosefinaCYCLOOXYGENASE-1CYCLOOXYGENASE-2DENTAL PULPMETALLOPROTEINASE-3MUSCARINIC ACETYLCHOLINE RECEPTORNITRIC OXIDE SYNTHASEPILOCARPINEPROSTAGLANDIN E2PULPITIShttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3The purpose of this study was to investigate the role of muscarinic acetylcholine receptor (mAChR) activity in the regulation of inducible nitric oxide synthase (iNOS) activity, prostaglandin E2 (PGE2), and metalloproteinase-3 (MMP-3) in experimentally induced inflammation of rat incisors dental pulp. Inflammation was induced by application of bacterial lipopolysaccharide (LPS) to the pulp. Extirpated pulp tissue samples were incubated in saline solution until the various experiments were performed. Saline-treated pulp and healthy pulp were used as controls. NOS activity was measured by the production of [U-14C]-citrulline from [U-14C]-arginine. PGE2 and MMP-3 production were evaluated by an enzyme-linked immunosorbent assay (ELISA) and cyclooxygenase (cox-1 and cox-2) messenger RNA levels were measured using a reverse-transcriptase polymerase chain reaction by coamplification of target complementary DNA with a single set of primers. The application of LPS to the pulp increased NOS activity, PGE2, and MMP-3 production associated with iNOS overactivity. Moreover, PGE2 and MMP-3 production were the result of cox-2 expression. Pilocarpine (5 × 10-11 mol/L to 5 × 10-9 mol/L), acting on mAChRs, triggered a negative effect on NOS activity, PGE2, and MMP-3 production. In control pulp, no action of pilocarpine was observed. Pulpitis changed mAChR conformation, increasing its coupling efficiency to transducing molecules that in turn activate iNOS. The capacity of pilocarpine to prevent iNOS activity, PGE2, and MMP-3 by acting on mAChR mutation induced by pulpitis might be useful therapeutically as a local treatment.Fil: de Couto Pita, Alejandra Karina. Universidad de Buenos Aires. Facultad de Odontología. Cátedra de Farmacología; ArgentinaFil: Borda, Enri Santiago. Universidad de Buenos Aires. Facultad de Odontología. Cátedra de Farmacología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; ArgentinaFil: Ganzinelli, Sabrina Belen. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina. Universidad de Buenos Aires. Facultad de Odontología. Cátedra de Farmacología; ArgentinaFil: Passafaro, Daniela. Universidad de Buenos Aires. Facultad de Odontología. Cátedra de Farmacología; ArgentinaFil: Sterin, Leonor Josefina. Universidad de Buenos Aires. Facultad de Odontología. Cátedra de Farmacología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; ArgentinaElsevier Science Inc2009-04info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/114224de Couto Pita, Alejandra Karina; Borda, Enri Santiago; Ganzinelli, Sabrina Belen; Passafaro, Daniela; Sterin, Leonor Josefina; Cholinoceptor Modulation on Nitric Oxide Regulates Prostaglandin E2 and Metalloproteinase-3 Production in Experimentally Induced Inflammation of Rat Dental Pulp; Elsevier Science Inc; Journal Of Endodontics; 35; 4; 4-2009; 529-5360099-2399CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/abs/pii/S009923990900051Xinfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.joen.2009.01.004info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:08:25Zoai:ri.conicet.gov.ar:11336/114224instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:08:26.187CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Cholinoceptor Modulation on Nitric Oxide Regulates Prostaglandin E2 and Metalloproteinase-3 Production in Experimentally Induced Inflammation of Rat Dental Pulp
title Cholinoceptor Modulation on Nitric Oxide Regulates Prostaglandin E2 and Metalloproteinase-3 Production in Experimentally Induced Inflammation of Rat Dental Pulp
spellingShingle Cholinoceptor Modulation on Nitric Oxide Regulates Prostaglandin E2 and Metalloproteinase-3 Production in Experimentally Induced Inflammation of Rat Dental Pulp
de Couto Pita, Alejandra Karina
CYCLOOXYGENASE-1
CYCLOOXYGENASE-2
DENTAL PULP
METALLOPROTEINASE-3
MUSCARINIC ACETYLCHOLINE RECEPTOR
NITRIC OXIDE SYNTHASE
PILOCARPINE
PROSTAGLANDIN E2
PULPITIS
title_short Cholinoceptor Modulation on Nitric Oxide Regulates Prostaglandin E2 and Metalloproteinase-3 Production in Experimentally Induced Inflammation of Rat Dental Pulp
title_full Cholinoceptor Modulation on Nitric Oxide Regulates Prostaglandin E2 and Metalloproteinase-3 Production in Experimentally Induced Inflammation of Rat Dental Pulp
title_fullStr Cholinoceptor Modulation on Nitric Oxide Regulates Prostaglandin E2 and Metalloproteinase-3 Production in Experimentally Induced Inflammation of Rat Dental Pulp
title_full_unstemmed Cholinoceptor Modulation on Nitric Oxide Regulates Prostaglandin E2 and Metalloproteinase-3 Production in Experimentally Induced Inflammation of Rat Dental Pulp
title_sort Cholinoceptor Modulation on Nitric Oxide Regulates Prostaglandin E2 and Metalloproteinase-3 Production in Experimentally Induced Inflammation of Rat Dental Pulp
dc.creator.none.fl_str_mv de Couto Pita, Alejandra Karina
Borda, Enri Santiago
Ganzinelli, Sabrina Belen
Passafaro, Daniela
Sterin, Leonor Josefina
author de Couto Pita, Alejandra Karina
author_facet de Couto Pita, Alejandra Karina
Borda, Enri Santiago
Ganzinelli, Sabrina Belen
Passafaro, Daniela
Sterin, Leonor Josefina
author_role author
author2 Borda, Enri Santiago
Ganzinelli, Sabrina Belen
Passafaro, Daniela
Sterin, Leonor Josefina
author2_role author
author
author
author
dc.subject.none.fl_str_mv CYCLOOXYGENASE-1
CYCLOOXYGENASE-2
DENTAL PULP
METALLOPROTEINASE-3
MUSCARINIC ACETYLCHOLINE RECEPTOR
NITRIC OXIDE SYNTHASE
PILOCARPINE
PROSTAGLANDIN E2
PULPITIS
topic CYCLOOXYGENASE-1
CYCLOOXYGENASE-2
DENTAL PULP
METALLOPROTEINASE-3
MUSCARINIC ACETYLCHOLINE RECEPTOR
NITRIC OXIDE SYNTHASE
PILOCARPINE
PROSTAGLANDIN E2
PULPITIS
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv The purpose of this study was to investigate the role of muscarinic acetylcholine receptor (mAChR) activity in the regulation of inducible nitric oxide synthase (iNOS) activity, prostaglandin E2 (PGE2), and metalloproteinase-3 (MMP-3) in experimentally induced inflammation of rat incisors dental pulp. Inflammation was induced by application of bacterial lipopolysaccharide (LPS) to the pulp. Extirpated pulp tissue samples were incubated in saline solution until the various experiments were performed. Saline-treated pulp and healthy pulp were used as controls. NOS activity was measured by the production of [U-14C]-citrulline from [U-14C]-arginine. PGE2 and MMP-3 production were evaluated by an enzyme-linked immunosorbent assay (ELISA) and cyclooxygenase (cox-1 and cox-2) messenger RNA levels were measured using a reverse-transcriptase polymerase chain reaction by coamplification of target complementary DNA with a single set of primers. The application of LPS to the pulp increased NOS activity, PGE2, and MMP-3 production associated with iNOS overactivity. Moreover, PGE2 and MMP-3 production were the result of cox-2 expression. Pilocarpine (5 × 10-11 mol/L to 5 × 10-9 mol/L), acting on mAChRs, triggered a negative effect on NOS activity, PGE2, and MMP-3 production. In control pulp, no action of pilocarpine was observed. Pulpitis changed mAChR conformation, increasing its coupling efficiency to transducing molecules that in turn activate iNOS. The capacity of pilocarpine to prevent iNOS activity, PGE2, and MMP-3 by acting on mAChR mutation induced by pulpitis might be useful therapeutically as a local treatment.
Fil: de Couto Pita, Alejandra Karina. Universidad de Buenos Aires. Facultad de Odontología. Cátedra de Farmacología; Argentina
Fil: Borda, Enri Santiago. Universidad de Buenos Aires. Facultad de Odontología. Cátedra de Farmacología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina
Fil: Ganzinelli, Sabrina Belen. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina. Universidad de Buenos Aires. Facultad de Odontología. Cátedra de Farmacología; Argentina
Fil: Passafaro, Daniela. Universidad de Buenos Aires. Facultad de Odontología. Cátedra de Farmacología; Argentina
Fil: Sterin, Leonor Josefina. Universidad de Buenos Aires. Facultad de Odontología. Cátedra de Farmacología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina
description The purpose of this study was to investigate the role of muscarinic acetylcholine receptor (mAChR) activity in the regulation of inducible nitric oxide synthase (iNOS) activity, prostaglandin E2 (PGE2), and metalloproteinase-3 (MMP-3) in experimentally induced inflammation of rat incisors dental pulp. Inflammation was induced by application of bacterial lipopolysaccharide (LPS) to the pulp. Extirpated pulp tissue samples were incubated in saline solution until the various experiments were performed. Saline-treated pulp and healthy pulp were used as controls. NOS activity was measured by the production of [U-14C]-citrulline from [U-14C]-arginine. PGE2 and MMP-3 production were evaluated by an enzyme-linked immunosorbent assay (ELISA) and cyclooxygenase (cox-1 and cox-2) messenger RNA levels were measured using a reverse-transcriptase polymerase chain reaction by coamplification of target complementary DNA with a single set of primers. The application of LPS to the pulp increased NOS activity, PGE2, and MMP-3 production associated with iNOS overactivity. Moreover, PGE2 and MMP-3 production were the result of cox-2 expression. Pilocarpine (5 × 10-11 mol/L to 5 × 10-9 mol/L), acting on mAChRs, triggered a negative effect on NOS activity, PGE2, and MMP-3 production. In control pulp, no action of pilocarpine was observed. Pulpitis changed mAChR conformation, increasing its coupling efficiency to transducing molecules that in turn activate iNOS. The capacity of pilocarpine to prevent iNOS activity, PGE2, and MMP-3 by acting on mAChR mutation induced by pulpitis might be useful therapeutically as a local treatment.
publishDate 2009
dc.date.none.fl_str_mv 2009-04
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/114224
de Couto Pita, Alejandra Karina; Borda, Enri Santiago; Ganzinelli, Sabrina Belen; Passafaro, Daniela; Sterin, Leonor Josefina; Cholinoceptor Modulation on Nitric Oxide Regulates Prostaglandin E2 and Metalloproteinase-3 Production in Experimentally Induced Inflammation of Rat Dental Pulp; Elsevier Science Inc; Journal Of Endodontics; 35; 4; 4-2009; 529-536
0099-2399
CONICET Digital
CONICET
url http://hdl.handle.net/11336/114224
identifier_str_mv de Couto Pita, Alejandra Karina; Borda, Enri Santiago; Ganzinelli, Sabrina Belen; Passafaro, Daniela; Sterin, Leonor Josefina; Cholinoceptor Modulation on Nitric Oxide Regulates Prostaglandin E2 and Metalloproteinase-3 Production in Experimentally Induced Inflammation of Rat Dental Pulp; Elsevier Science Inc; Journal Of Endodontics; 35; 4; 4-2009; 529-536
0099-2399
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/abs/pii/S009923990900051X
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.joen.2009.01.004
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Science Inc
publisher.none.fl_str_mv Elsevier Science Inc
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1844613951551176704
score 13.070432

Publicaciones similares