Differential signalling pathways involved in cholinoceptor‐dependent stimulation of nitric oxide isoforms in dental pulp

Autores
Sterin, Leonor Josefina; Furlan, C.; Reina, Silvia Lorena; Orman, Betina; Borda, Enri Santiago
Año de publicación
2007
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
AIM: To investigate the role of muscarinic acetylcholine receptor (mAChR) subtype activity in the regulation of endothelial- (e) and neuronal- (n) nitric oxide synthase (NOS) expression and activity. METHODOLOGY: Rat dental pulp tissue was used throughout the study. The e-nos and n-nos mRNA levels were specifically measured using reverse transcriptase polymerase chain reaction procedures that involve simultaneous co-amplification of both target cDNA and a reference template with a single set of primers. NOS activity was measured by the production of [U-(14)C]-citrulline from [U-(14)C]-arginine. RESULTS: Stimulation of M(1)/M(2) and M(3)/M(4) mAChRs with pilocarpine caused an increase in e-nos and n-nos mRNA levels and NOS activity in the dental pulp. The specific mAChR subtype antagonists, L-NMMA, l-NIO and N(2)-propyl-L-arginine but not aminoguanidine attenuated all these effects. Inhibitors of phospholipase C (PLC), protein kinase C (PKC) and calcium/calmodulin (CaM) prevented the pilocarpine-dependent increase in n-nos and e-nos mRNA levels and NOS activity. CONCLUSIONS: Activation of mAChR subtypes stimulated NOS activity by increasing the production of NO through e-nos and n-nos gene expression and NOS activity. The mechanism appears to occur secondarily to stimulation of CaM and PKC enzymatic activity.
Fil: Sterin, Leonor Josefina. Universidad de Buenos Aires. Facultad de Medicina. Cátedra de Farmacología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina
Fil: Furlan, C.. Universidad de Buenos Aires. Facultad de Medicina. Cátedra de Farmacología; Argentina
Fil: Reina, Silvia Lorena. Universidad de Buenos Aires. Facultad de Medicina. Cátedra de Farmacología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina
Fil: Orman, Betina. Universidad de Buenos Aires. Facultad de Medicina. Cátedra de Farmacología; Argentina
Fil: Borda, Enri Santiago. Universidad de Buenos Aires. Facultad de Medicina. Cátedra de Farmacología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina
Materia
dental pulp
n, NOS, e-nos mNRNA
nitric oxide synthase
pilocarpine
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/241728
Acceder
id CONICETDig_c02b9bde4166b97e2a9a6121573ae3b7
oai_identifier_str oai:ri.conicet.gov.ar:11336/241728
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Differential signalling pathways involved in cholinoceptor‐dependent stimulation of nitric oxide isoforms in dental pulpSterin, Leonor JosefinaFurlan, C.Reina, Silvia LorenaOrman, BetinaBorda, Enri Santiagodental pulpn, NOS, e-nos mNRNAnitric oxide synthasepilocarpinehttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3AIM: To investigate the role of muscarinic acetylcholine receptor (mAChR) subtype activity in the regulation of endothelial- (e) and neuronal- (n) nitric oxide synthase (NOS) expression and activity. METHODOLOGY: Rat dental pulp tissue was used throughout the study. The e-nos and n-nos mRNA levels were specifically measured using reverse transcriptase polymerase chain reaction procedures that involve simultaneous co-amplification of both target cDNA and a reference template with a single set of primers. NOS activity was measured by the production of [U-(14)C]-citrulline from [U-(14)C]-arginine. RESULTS: Stimulation of M(1)/M(2) and M(3)/M(4) mAChRs with pilocarpine caused an increase in e-nos and n-nos mRNA levels and NOS activity in the dental pulp. The specific mAChR subtype antagonists, L-NMMA, l-NIO and N(2)-propyl-L-arginine but not aminoguanidine attenuated all these effects. Inhibitors of phospholipase C (PLC), protein kinase C (PKC) and calcium/calmodulin (CaM) prevented the pilocarpine-dependent increase in n-nos and e-nos mRNA levels and NOS activity. CONCLUSIONS: Activation of mAChR subtypes stimulated NOS activity by increasing the production of NO through e-nos and n-nos gene expression and NOS activity. The mechanism appears to occur secondarily to stimulation of CaM and PKC enzymatic activity.Fil: Sterin, Leonor Josefina. Universidad de Buenos Aires. Facultad de Medicina. Cátedra de Farmacología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; ArgentinaFil: Furlan, C.. Universidad de Buenos Aires. Facultad de Medicina. Cátedra de Farmacología; ArgentinaFil: Reina, Silvia Lorena. Universidad de Buenos Aires. Facultad de Medicina. Cátedra de Farmacología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; ArgentinaFil: Orman, Betina. Universidad de Buenos Aires. Facultad de Medicina. Cátedra de Farmacología; ArgentinaFil: Borda, Enri Santiago. Universidad de Buenos Aires. Facultad de Medicina. Cátedra de Farmacología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; ArgentinaWiley Blackwell Publishing, Inc2007-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/241728Sterin, Leonor Josefina; Furlan, C.; Reina, Silvia Lorena; Orman, Betina; Borda, Enri Santiago; Differential signalling pathways involved in cholinoceptor‐dependent stimulation of nitric oxide isoforms in dental pulp; Wiley Blackwell Publishing, Inc; International Endodontic Journal; 40; 7; 12-2007; 544-5520143-2885CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/full/10.1111/j.1365-2591.2007.01259.xinfo:eu-repo/semantics/altIdentifier/doi/10.1111/j.1365-2591.2007.01259.xinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:42:30Zoai:ri.conicet.gov.ar:11336/241728instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:42:31.169CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Differential signalling pathways involved in cholinoceptor‐dependent stimulation of nitric oxide isoforms in dental pulp
title Differential signalling pathways involved in cholinoceptor‐dependent stimulation of nitric oxide isoforms in dental pulp
spellingShingle Differential signalling pathways involved in cholinoceptor‐dependent stimulation of nitric oxide isoforms in dental pulp
Sterin, Leonor Josefina
dental pulp
n, NOS, e-nos mNRNA
nitric oxide synthase
pilocarpine
title_short Differential signalling pathways involved in cholinoceptor‐dependent stimulation of nitric oxide isoforms in dental pulp
title_full Differential signalling pathways involved in cholinoceptor‐dependent stimulation of nitric oxide isoforms in dental pulp
title_fullStr Differential signalling pathways involved in cholinoceptor‐dependent stimulation of nitric oxide isoforms in dental pulp
title_full_unstemmed Differential signalling pathways involved in cholinoceptor‐dependent stimulation of nitric oxide isoforms in dental pulp
title_sort Differential signalling pathways involved in cholinoceptor‐dependent stimulation of nitric oxide isoforms in dental pulp
dc.creator.none.fl_str_mv Sterin, Leonor Josefina
Furlan, C.
Reina, Silvia Lorena
Orman, Betina
Borda, Enri Santiago
author Sterin, Leonor Josefina
author_facet Sterin, Leonor Josefina
Furlan, C.
Reina, Silvia Lorena
Orman, Betina
Borda, Enri Santiago
author_role author
author2 Furlan, C.
Reina, Silvia Lorena
Orman, Betina
Borda, Enri Santiago
author2_role author
author
author
author
dc.subject.none.fl_str_mv dental pulp
n, NOS, e-nos mNRNA
nitric oxide synthase
pilocarpine
topic dental pulp
n, NOS, e-nos mNRNA
nitric oxide synthase
pilocarpine
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv AIM: To investigate the role of muscarinic acetylcholine receptor (mAChR) subtype activity in the regulation of endothelial- (e) and neuronal- (n) nitric oxide synthase (NOS) expression and activity. METHODOLOGY: Rat dental pulp tissue was used throughout the study. The e-nos and n-nos mRNA levels were specifically measured using reverse transcriptase polymerase chain reaction procedures that involve simultaneous co-amplification of both target cDNA and a reference template with a single set of primers. NOS activity was measured by the production of [U-(14)C]-citrulline from [U-(14)C]-arginine. RESULTS: Stimulation of M(1)/M(2) and M(3)/M(4) mAChRs with pilocarpine caused an increase in e-nos and n-nos mRNA levels and NOS activity in the dental pulp. The specific mAChR subtype antagonists, L-NMMA, l-NIO and N(2)-propyl-L-arginine but not aminoguanidine attenuated all these effects. Inhibitors of phospholipase C (PLC), protein kinase C (PKC) and calcium/calmodulin (CaM) prevented the pilocarpine-dependent increase in n-nos and e-nos mRNA levels and NOS activity. CONCLUSIONS: Activation of mAChR subtypes stimulated NOS activity by increasing the production of NO through e-nos and n-nos gene expression and NOS activity. The mechanism appears to occur secondarily to stimulation of CaM and PKC enzymatic activity.
Fil: Sterin, Leonor Josefina. Universidad de Buenos Aires. Facultad de Medicina. Cátedra de Farmacología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina
Fil: Furlan, C.. Universidad de Buenos Aires. Facultad de Medicina. Cátedra de Farmacología; Argentina
Fil: Reina, Silvia Lorena. Universidad de Buenos Aires. Facultad de Medicina. Cátedra de Farmacología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina
Fil: Orman, Betina. Universidad de Buenos Aires. Facultad de Medicina. Cátedra de Farmacología; Argentina
Fil: Borda, Enri Santiago. Universidad de Buenos Aires. Facultad de Medicina. Cátedra de Farmacología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina
description AIM: To investigate the role of muscarinic acetylcholine receptor (mAChR) subtype activity in the regulation of endothelial- (e) and neuronal- (n) nitric oxide synthase (NOS) expression and activity. METHODOLOGY: Rat dental pulp tissue was used throughout the study. The e-nos and n-nos mRNA levels were specifically measured using reverse transcriptase polymerase chain reaction procedures that involve simultaneous co-amplification of both target cDNA and a reference template with a single set of primers. NOS activity was measured by the production of [U-(14)C]-citrulline from [U-(14)C]-arginine. RESULTS: Stimulation of M(1)/M(2) and M(3)/M(4) mAChRs with pilocarpine caused an increase in e-nos and n-nos mRNA levels and NOS activity in the dental pulp. The specific mAChR subtype antagonists, L-NMMA, l-NIO and N(2)-propyl-L-arginine but not aminoguanidine attenuated all these effects. Inhibitors of phospholipase C (PLC), protein kinase C (PKC) and calcium/calmodulin (CaM) prevented the pilocarpine-dependent increase in n-nos and e-nos mRNA levels and NOS activity. CONCLUSIONS: Activation of mAChR subtypes stimulated NOS activity by increasing the production of NO through e-nos and n-nos gene expression and NOS activity. The mechanism appears to occur secondarily to stimulation of CaM and PKC enzymatic activity.
publishDate 2007
dc.date.none.fl_str_mv 2007-12
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/241728
Sterin, Leonor Josefina; Furlan, C.; Reina, Silvia Lorena; Orman, Betina; Borda, Enri Santiago; Differential signalling pathways involved in cholinoceptor‐dependent stimulation of nitric oxide isoforms in dental pulp; Wiley Blackwell Publishing, Inc; International Endodontic Journal; 40; 7; 12-2007; 544-552
0143-2885
CONICET Digital
CONICET
url http://hdl.handle.net/11336/241728
identifier_str_mv Sterin, Leonor Josefina; Furlan, C.; Reina, Silvia Lorena; Orman, Betina; Borda, Enri Santiago; Differential signalling pathways involved in cholinoceptor‐dependent stimulation of nitric oxide isoforms in dental pulp; Wiley Blackwell Publishing, Inc; International Endodontic Journal; 40; 7; 12-2007; 544-552
0143-2885
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/full/10.1111/j.1365-2591.2007.01259.x
info:eu-repo/semantics/altIdentifier/doi/10.1111/j.1365-2591.2007.01259.x
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Wiley Blackwell Publishing, Inc
publisher.none.fl_str_mv Wiley Blackwell Publishing, Inc
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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