FTIR and Raman characterization of DMPC lipid vesicles with different biomolecules

Autores
Corrales Chahar, Fernanda; Lacosegliaz, Mariano José; Sanini, M. V.; Diaz, Silvia Beatriz; Ben Altabef, Aida; Gervasi, Claudio; Álvarez, P.
Año de publicación
2016
Idioma
inglés
Tipo de recurso
documento de conferencia
Estado
versión publicada
Descripción
This work aims at gaining insight into the characteristics of the membrane-biomolecule interaction in order to understand the action mechanisms of the biomolecules, their effectivity and physicochemical properties. Vitamin C (ascorbic acid) is an essential cofactor for certain enzimes that are widely used in medicine and in food industry, annonacin is a mono-THF acetogenin a neurotoxic chemical compound found in some fruits with potential applicability as insecticide, acaricide, fungicide, as well as antiparasitic and antitumor agents, finally, valproic acid is an antiepileptic drug and mood stabilizer with action on different channels of the central nervous system. 1,2-Dimyristoyl-sn-glycero-3-phosphocholine (DMPC) multilamellar vesicles were prepared in the presence of different concentrations of the above-mentioned biomolecules. FTIR and Raman spectroscopies were used to analyse the participation of specific functional groups in the biomolecule-membrane interaction. Spectra recorded for the complexes Biomolecule:DMPC were analysed in comparison with those of the pure liquid, studying spectral bands related to the inner regions of the lipid bilayer (hydrophobic regions) and the interphasial region (hydrophilic region). Low and high-frequency shifts were observed depending on the group and the temperature. The effect of each biomolecule on the transition temperature (Tm) the phase gel transition (Lβ) – liquid crystalline (Lα) of the phospholipids exhibits a feature transition. Raman spectra show variations according to the presence of ascorbica acid, valproic acid or annonacin.
Fil: Corrales Chahar, Fernanda. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Instituto de Física; Argentina
Fil: Lacosegliaz, Mariano José. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Instituto de Física; Argentina
Fil: Sanini, M. V.. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Instituto de Física; Argentina
Fil: Diaz, Silvia Beatriz. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; Argentina
Fil: Ben Altabef, Aida. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto de Química del Noroeste. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Instituto de Química del Noroeste; Argentina
Fil: Gervasi, Claudio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas; Argentina
Fil: Álvarez, P.. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto de Química del Noroeste. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Instituto de Química del Noroeste; Argentina
III Latin American Federation of Biophysical Societies; IX IberoAmerican Congress of Biophysics; XLV Reunión Anual Sociedad Argentina de Biofísica
San Miguel de Tucumán
Argentina
Sociedad Argentina de Biofísica
Materia
DMPC lipid vesicles
Biomolecules
FTIR
Raman
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/237378
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/237378
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling FTIR and Raman characterization of DMPC lipid vesicles with different biomoleculesCorrales Chahar, FernandaLacosegliaz, Mariano JoséSanini, M. V.Diaz, Silvia BeatrizBen Altabef, AidaGervasi, ClaudioÁlvarez, P.DMPC lipid vesiclesBiomoleculesFTIRRamanhttps://purl.org/becyt/ford/1.4https://purl.org/becyt/ford/1This work aims at gaining insight into the characteristics of the membrane-biomolecule interaction in order to understand the action mechanisms of the biomolecules, their effectivity and physicochemical properties. Vitamin C (ascorbic acid) is an essential cofactor for certain enzimes that are widely used in medicine and in food industry, annonacin is a mono-THF acetogenin a neurotoxic chemical compound found in some fruits with potential applicability as insecticide, acaricide, fungicide, as well as antiparasitic and antitumor agents, finally, valproic acid is an antiepileptic drug and mood stabilizer with action on different channels of the central nervous system. 1,2-Dimyristoyl-sn-glycero-3-phosphocholine (DMPC) multilamellar vesicles were prepared in the presence of different concentrations of the above-mentioned biomolecules. FTIR and Raman spectroscopies were used to analyse the participation of specific functional groups in the biomolecule-membrane interaction. Spectra recorded for the complexes Biomolecule:DMPC were analysed in comparison with those of the pure liquid, studying spectral bands related to the inner regions of the lipid bilayer (hydrophobic regions) and the interphasial region (hydrophilic region). Low and high-frequency shifts were observed depending on the group and the temperature. The effect of each biomolecule on the transition temperature (Tm) the phase gel transition (Lβ) – liquid crystalline (Lα) of the phospholipids exhibits a feature transition. Raman spectra show variations according to the presence of ascorbica acid, valproic acid or annonacin.Fil: Corrales Chahar, Fernanda. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Instituto de Física; ArgentinaFil: Lacosegliaz, Mariano José. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Instituto de Física; ArgentinaFil: Sanini, M. V.. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Instituto de Física; ArgentinaFil: Diaz, Silvia Beatriz. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; ArgentinaFil: Ben Altabef, Aida. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto de Química del Noroeste. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Instituto de Química del Noroeste; ArgentinaFil: Gervasi, Claudio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas; ArgentinaFil: Álvarez, P.. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto de Química del Noroeste. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Instituto de Química del Noroeste; ArgentinaIII Latin American Federation of Biophysical Societies; IX IberoAmerican Congress of Biophysics; XLV Reunión Anual Sociedad Argentina de BiofísicaSan Miguel de TucumánArgentinaSociedad Argentina de BiofísicaSociedad Argentina de Biofísica2016info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectCongresoBookhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/mswordapplication/pdfhttp://hdl.handle.net/11336/237378FTIR and Raman characterization of DMPC lipid vesicles with different biomolecules; III Latin American Federation of Biophysical Societies; IX IberoAmerican Congress of Biophysics; XLV Reunión Anual Sociedad Argentina de Biofísica; San Miguel de Tucumán; Argentina; 2016; 358-360CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://biofisica.org.ar/publicaciones/libros-de-resumenes/Nacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:51:10Zoai:ri.conicet.gov.ar:11336/237378instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:51:10.752CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv FTIR and Raman characterization of DMPC lipid vesicles with different biomolecules
title FTIR and Raman characterization of DMPC lipid vesicles with different biomolecules
spellingShingle FTIR and Raman characterization of DMPC lipid vesicles with different biomolecules
Corrales Chahar, Fernanda
DMPC lipid vesicles
Biomolecules
FTIR
Raman
title_short FTIR and Raman characterization of DMPC lipid vesicles with different biomolecules
title_full FTIR and Raman characterization of DMPC lipid vesicles with different biomolecules
title_fullStr FTIR and Raman characterization of DMPC lipid vesicles with different biomolecules
title_full_unstemmed FTIR and Raman characterization of DMPC lipid vesicles with different biomolecules
title_sort FTIR and Raman characterization of DMPC lipid vesicles with different biomolecules
dc.creator.none.fl_str_mv Corrales Chahar, Fernanda
Lacosegliaz, Mariano José
Sanini, M. V.
Diaz, Silvia Beatriz
Ben Altabef, Aida
Gervasi, Claudio
Álvarez, P.
author Corrales Chahar, Fernanda
author_facet Corrales Chahar, Fernanda
Lacosegliaz, Mariano José
Sanini, M. V.
Diaz, Silvia Beatriz
Ben Altabef, Aida
Gervasi, Claudio
Álvarez, P.
author_role author
author2 Lacosegliaz, Mariano José
Sanini, M. V.
Diaz, Silvia Beatriz
Ben Altabef, Aida
Gervasi, Claudio
Álvarez, P.
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv DMPC lipid vesicles
Biomolecules
FTIR
Raman
topic DMPC lipid vesicles
Biomolecules
FTIR
Raman
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.4
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv This work aims at gaining insight into the characteristics of the membrane-biomolecule interaction in order to understand the action mechanisms of the biomolecules, their effectivity and physicochemical properties. Vitamin C (ascorbic acid) is an essential cofactor for certain enzimes that are widely used in medicine and in food industry, annonacin is a mono-THF acetogenin a neurotoxic chemical compound found in some fruits with potential applicability as insecticide, acaricide, fungicide, as well as antiparasitic and antitumor agents, finally, valproic acid is an antiepileptic drug and mood stabilizer with action on different channels of the central nervous system. 1,2-Dimyristoyl-sn-glycero-3-phosphocholine (DMPC) multilamellar vesicles were prepared in the presence of different concentrations of the above-mentioned biomolecules. FTIR and Raman spectroscopies were used to analyse the participation of specific functional groups in the biomolecule-membrane interaction. Spectra recorded for the complexes Biomolecule:DMPC were analysed in comparison with those of the pure liquid, studying spectral bands related to the inner regions of the lipid bilayer (hydrophobic regions) and the interphasial region (hydrophilic region). Low and high-frequency shifts were observed depending on the group and the temperature. The effect of each biomolecule on the transition temperature (Tm) the phase gel transition (Lβ) – liquid crystalline (Lα) of the phospholipids exhibits a feature transition. Raman spectra show variations according to the presence of ascorbica acid, valproic acid or annonacin.
Fil: Corrales Chahar, Fernanda. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Instituto de Física; Argentina
Fil: Lacosegliaz, Mariano José. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Instituto de Física; Argentina
Fil: Sanini, M. V.. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Instituto de Física; Argentina
Fil: Diaz, Silvia Beatriz. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; Argentina
Fil: Ben Altabef, Aida. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto de Química del Noroeste. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Instituto de Química del Noroeste; Argentina
Fil: Gervasi, Claudio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas; Argentina
Fil: Álvarez, P.. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto de Química del Noroeste. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Instituto de Química del Noroeste; Argentina
III Latin American Federation of Biophysical Societies; IX IberoAmerican Congress of Biophysics; XLV Reunión Anual Sociedad Argentina de Biofísica
San Miguel de Tucumán
Argentina
Sociedad Argentina de Biofísica
description This work aims at gaining insight into the characteristics of the membrane-biomolecule interaction in order to understand the action mechanisms of the biomolecules, their effectivity and physicochemical properties. Vitamin C (ascorbic acid) is an essential cofactor for certain enzimes that are widely used in medicine and in food industry, annonacin is a mono-THF acetogenin a neurotoxic chemical compound found in some fruits with potential applicability as insecticide, acaricide, fungicide, as well as antiparasitic and antitumor agents, finally, valproic acid is an antiepileptic drug and mood stabilizer with action on different channels of the central nervous system. 1,2-Dimyristoyl-sn-glycero-3-phosphocholine (DMPC) multilamellar vesicles were prepared in the presence of different concentrations of the above-mentioned biomolecules. FTIR and Raman spectroscopies were used to analyse the participation of specific functional groups in the biomolecule-membrane interaction. Spectra recorded for the complexes Biomolecule:DMPC were analysed in comparison with those of the pure liquid, studying spectral bands related to the inner regions of the lipid bilayer (hydrophobic regions) and the interphasial region (hydrophilic region). Low and high-frequency shifts were observed depending on the group and the temperature. The effect of each biomolecule on the transition temperature (Tm) the phase gel transition (Lβ) – liquid crystalline (Lα) of the phospholipids exhibits a feature transition. Raman spectra show variations according to the presence of ascorbica acid, valproic acid or annonacin.
publishDate 2016
dc.date.none.fl_str_mv 2016
dc.type.none.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/conferenceObject
Congreso
Book
http://purl.org/coar/resource_type/c_5794
info:ar-repo/semantics/documentoDeConferencia
status_str publishedVersion
format conferenceObject
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/237378
FTIR and Raman characterization of DMPC lipid vesicles with different biomolecules; III Latin American Federation of Biophysical Societies; IX IberoAmerican Congress of Biophysics; XLV Reunión Anual Sociedad Argentina de Biofísica; San Miguel de Tucumán; Argentina; 2016; 358-360
CONICET Digital
CONICET
url http://hdl.handle.net/11336/237378
identifier_str_mv FTIR and Raman characterization of DMPC lipid vesicles with different biomolecules; III Latin American Federation of Biophysical Societies; IX IberoAmerican Congress of Biophysics; XLV Reunión Anual Sociedad Argentina de Biofísica; San Miguel de Tucumán; Argentina; 2016; 358-360
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://biofisica.org.ar/publicaciones/libros-de-resumenes/
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/msword
application/pdf
dc.coverage.none.fl_str_mv Nacional
dc.publisher.none.fl_str_mv Sociedad Argentina de Biofísica
publisher.none.fl_str_mv Sociedad Argentina de Biofísica
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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