Characterization of interactions of eggPC lipid structures with different biomolecules
- Autores
- Corrales Chahar, F.; Díaz, S.B.; Ben Altabef, Aida; Gervasi, Claudio; Alvarez, Patricia Eugenia
- Año de publicación
- 2018
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- In this paper we study the interactions of two biomolecules (ascorbic acid and Annonacin) with a bilayer lipid membrane. Egg yolk phosphatidylcholine (eggPC) liposomes (in crystalline liquid state) were prepared in solutions of ascorbic acid (AA) at different concentration levels. On the other hand, liposomes were doped with Annonacin (Ann), a mono-tetrahydrofuran acetogenin (ACG), which is an effective citotoxic substance. While AA pharmacologic effect and action mechanisms are widely known, those of Ann's are only very recently being studied. Both Fourier Transformed Infrared (FTIR) and Raman spectroscopic techniques were used to study the participation of the main functional groups of the lipid bilayer involved in the membrane-solution interaction. The obtained spectra were comparatively analyzed, studying the spectral bands corresponding to both the hydrophobic and the hydrophilic regions in the lipid bilayer. Electrochemical experiments namely; impedance spectroscopy (EIS) and cyclic voltamperometry (CV) were used as the main characterization techniques to analyse stability and structural changes of a model system of supported EggPC bilayer in connection with its interactions with AA and Ann. At high molar ratios of AA, there is dehydration in both populations of the carbonyl group of the polar head of the lipid. On the other hand, Ann promotes the formation of hydrogen bonds with the carbonyl groups. No interaction between AA and phosphate groups is observed at low and intermediate molar ratios. Ann is expected to be able to induce the dehydration of the phosphate groups without the subsequent formation of H bonds with them. According to the electrochemical analysis, the interaction of AA with the supported lipid membrane does not alter its dielectric properties. This fact can be related to the conservation of structured water of the phosphate groups in the polar heads of the lipid. On the other hand, the incorporation of Ann into the lipid membrane generates an increase in the number of defects while changes the dielectric constant. This, in turn, can be associated with the induced dehydration of the phosphate groups.
Fil: Corrales Chahar, F.. Universidad Nacional de Tucumán; Argentina
Fil: Díaz, S.B.. Universidad Nacional de Tucumán; Argentina
Fil: Ben Altabef, Aida. Universidad Nacional de Tucumán; Argentina. Instituto de Química del Noroeste Argentino (inquinoa)-conicet-tucumán; Argentina
Fil: Gervasi, Claudio. Facultad de Ingeniería, Universidad Nacional de la Plata; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas; Argentina
Fil: Alvarez, Patricia Eugenia. Universidad Nacional de Tucumán; Argentina. Instituto de Química del Noroeste Argentino (inquinoa)-conicet-tucumán; Argentina - Materia
-
ANNONACEOUS ACETOGENINS
ASCORBIC ACID
EIS VOLTAMPEROMETRY
FTIR
RAMAN SPECTROSCOPY - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
- Repositorio
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/97183
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Characterization of interactions of eggPC lipid structures with different biomoleculesCorrales Chahar, F.Díaz, S.B.Ben Altabef, AidaGervasi, ClaudioAlvarez, Patricia EugeniaANNONACEOUS ACETOGENINSASCORBIC ACIDEIS VOLTAMPEROMETRYFTIRRAMAN SPECTROSCOPYhttps://purl.org/becyt/ford/1.4https://purl.org/becyt/ford/1In this paper we study the interactions of two biomolecules (ascorbic acid and Annonacin) with a bilayer lipid membrane. Egg yolk phosphatidylcholine (eggPC) liposomes (in crystalline liquid state) were prepared in solutions of ascorbic acid (AA) at different concentration levels. On the other hand, liposomes were doped with Annonacin (Ann), a mono-tetrahydrofuran acetogenin (ACG), which is an effective citotoxic substance. While AA pharmacologic effect and action mechanisms are widely known, those of Ann's are only very recently being studied. Both Fourier Transformed Infrared (FTIR) and Raman spectroscopic techniques were used to study the participation of the main functional groups of the lipid bilayer involved in the membrane-solution interaction. The obtained spectra were comparatively analyzed, studying the spectral bands corresponding to both the hydrophobic and the hydrophilic regions in the lipid bilayer. Electrochemical experiments namely; impedance spectroscopy (EIS) and cyclic voltamperometry (CV) were used as the main characterization techniques to analyse stability and structural changes of a model system of supported EggPC bilayer in connection with its interactions with AA and Ann. At high molar ratios of AA, there is dehydration in both populations of the carbonyl group of the polar head of the lipid. On the other hand, Ann promotes the formation of hydrogen bonds with the carbonyl groups. No interaction between AA and phosphate groups is observed at low and intermediate molar ratios. Ann is expected to be able to induce the dehydration of the phosphate groups without the subsequent formation of H bonds with them. According to the electrochemical analysis, the interaction of AA with the supported lipid membrane does not alter its dielectric properties. This fact can be related to the conservation of structured water of the phosphate groups in the polar heads of the lipid. On the other hand, the incorporation of Ann into the lipid membrane generates an increase in the number of defects while changes the dielectric constant. This, in turn, can be associated with the induced dehydration of the phosphate groups.Fil: Corrales Chahar, F.. Universidad Nacional de Tucumán; ArgentinaFil: Díaz, S.B.. Universidad Nacional de Tucumán; ArgentinaFil: Ben Altabef, Aida. Universidad Nacional de Tucumán; Argentina. Instituto de Química del Noroeste Argentino (inquinoa)-conicet-tucumán; ArgentinaFil: Gervasi, Claudio. Facultad de Ingeniería, Universidad Nacional de la Plata; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas; ArgentinaFil: Alvarez, Patricia Eugenia. Universidad Nacional de Tucumán; Argentina. Instituto de Química del Noroeste Argentino (inquinoa)-conicet-tucumán; ArgentinaElsevier Ireland2018-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/97183Corrales Chahar, F.; Díaz, S.B.; Ben Altabef, Aida; Gervasi, Claudio; Alvarez, Patricia Eugenia; Characterization of interactions of eggPC lipid structures with different biomolecules; Elsevier Ireland; Chemistry And Physics Of Lipids; 210; 1-2018; 60-690009-3084CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.chemphyslip.2017.11.013info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0009308417302517?via%3Dihubinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-22T11:19:29Zoai:ri.conicet.gov.ar:11336/97183instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-22 11:19:29.808CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
dc.title.none.fl_str_mv |
Characterization of interactions of eggPC lipid structures with different biomolecules |
title |
Characterization of interactions of eggPC lipid structures with different biomolecules |
spellingShingle |
Characterization of interactions of eggPC lipid structures with different biomolecules Corrales Chahar, F. ANNONACEOUS ACETOGENINS ASCORBIC ACID EIS VOLTAMPEROMETRY FTIR RAMAN SPECTROSCOPY |
title_short |
Characterization of interactions of eggPC lipid structures with different biomolecules |
title_full |
Characterization of interactions of eggPC lipid structures with different biomolecules |
title_fullStr |
Characterization of interactions of eggPC lipid structures with different biomolecules |
title_full_unstemmed |
Characterization of interactions of eggPC lipid structures with different biomolecules |
title_sort |
Characterization of interactions of eggPC lipid structures with different biomolecules |
dc.creator.none.fl_str_mv |
Corrales Chahar, F. Díaz, S.B. Ben Altabef, Aida Gervasi, Claudio Alvarez, Patricia Eugenia |
author |
Corrales Chahar, F. |
author_facet |
Corrales Chahar, F. Díaz, S.B. Ben Altabef, Aida Gervasi, Claudio Alvarez, Patricia Eugenia |
author_role |
author |
author2 |
Díaz, S.B. Ben Altabef, Aida Gervasi, Claudio Alvarez, Patricia Eugenia |
author2_role |
author author author author |
dc.subject.none.fl_str_mv |
ANNONACEOUS ACETOGENINS ASCORBIC ACID EIS VOLTAMPEROMETRY FTIR RAMAN SPECTROSCOPY |
topic |
ANNONACEOUS ACETOGENINS ASCORBIC ACID EIS VOLTAMPEROMETRY FTIR RAMAN SPECTROSCOPY |
purl_subject.fl_str_mv |
https://purl.org/becyt/ford/1.4 https://purl.org/becyt/ford/1 |
dc.description.none.fl_txt_mv |
In this paper we study the interactions of two biomolecules (ascorbic acid and Annonacin) with a bilayer lipid membrane. Egg yolk phosphatidylcholine (eggPC) liposomes (in crystalline liquid state) were prepared in solutions of ascorbic acid (AA) at different concentration levels. On the other hand, liposomes were doped with Annonacin (Ann), a mono-tetrahydrofuran acetogenin (ACG), which is an effective citotoxic substance. While AA pharmacologic effect and action mechanisms are widely known, those of Ann's are only very recently being studied. Both Fourier Transformed Infrared (FTIR) and Raman spectroscopic techniques were used to study the participation of the main functional groups of the lipid bilayer involved in the membrane-solution interaction. The obtained spectra were comparatively analyzed, studying the spectral bands corresponding to both the hydrophobic and the hydrophilic regions in the lipid bilayer. Electrochemical experiments namely; impedance spectroscopy (EIS) and cyclic voltamperometry (CV) were used as the main characterization techniques to analyse stability and structural changes of a model system of supported EggPC bilayer in connection with its interactions with AA and Ann. At high molar ratios of AA, there is dehydration in both populations of the carbonyl group of the polar head of the lipid. On the other hand, Ann promotes the formation of hydrogen bonds with the carbonyl groups. No interaction between AA and phosphate groups is observed at low and intermediate molar ratios. Ann is expected to be able to induce the dehydration of the phosphate groups without the subsequent formation of H bonds with them. According to the electrochemical analysis, the interaction of AA with the supported lipid membrane does not alter its dielectric properties. This fact can be related to the conservation of structured water of the phosphate groups in the polar heads of the lipid. On the other hand, the incorporation of Ann into the lipid membrane generates an increase in the number of defects while changes the dielectric constant. This, in turn, can be associated with the induced dehydration of the phosphate groups. Fil: Corrales Chahar, F.. Universidad Nacional de Tucumán; Argentina Fil: Díaz, S.B.. Universidad Nacional de Tucumán; Argentina Fil: Ben Altabef, Aida. Universidad Nacional de Tucumán; Argentina. Instituto de Química del Noroeste Argentino (inquinoa)-conicet-tucumán; Argentina Fil: Gervasi, Claudio. Facultad de Ingeniería, Universidad Nacional de la Plata; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas; Argentina Fil: Alvarez, Patricia Eugenia. Universidad Nacional de Tucumán; Argentina. Instituto de Química del Noroeste Argentino (inquinoa)-conicet-tucumán; Argentina |
description |
In this paper we study the interactions of two biomolecules (ascorbic acid and Annonacin) with a bilayer lipid membrane. Egg yolk phosphatidylcholine (eggPC) liposomes (in crystalline liquid state) were prepared in solutions of ascorbic acid (AA) at different concentration levels. On the other hand, liposomes were doped with Annonacin (Ann), a mono-tetrahydrofuran acetogenin (ACG), which is an effective citotoxic substance. While AA pharmacologic effect and action mechanisms are widely known, those of Ann's are only very recently being studied. Both Fourier Transformed Infrared (FTIR) and Raman spectroscopic techniques were used to study the participation of the main functional groups of the lipid bilayer involved in the membrane-solution interaction. The obtained spectra were comparatively analyzed, studying the spectral bands corresponding to both the hydrophobic and the hydrophilic regions in the lipid bilayer. Electrochemical experiments namely; impedance spectroscopy (EIS) and cyclic voltamperometry (CV) were used as the main characterization techniques to analyse stability and structural changes of a model system of supported EggPC bilayer in connection with its interactions with AA and Ann. At high molar ratios of AA, there is dehydration in both populations of the carbonyl group of the polar head of the lipid. On the other hand, Ann promotes the formation of hydrogen bonds with the carbonyl groups. No interaction between AA and phosphate groups is observed at low and intermediate molar ratios. Ann is expected to be able to induce the dehydration of the phosphate groups without the subsequent formation of H bonds with them. According to the electrochemical analysis, the interaction of AA with the supported lipid membrane does not alter its dielectric properties. This fact can be related to the conservation of structured water of the phosphate groups in the polar heads of the lipid. On the other hand, the incorporation of Ann into the lipid membrane generates an increase in the number of defects while changes the dielectric constant. This, in turn, can be associated with the induced dehydration of the phosphate groups. |
publishDate |
2018 |
dc.date.none.fl_str_mv |
2018-01 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/97183 Corrales Chahar, F.; Díaz, S.B.; Ben Altabef, Aida; Gervasi, Claudio; Alvarez, Patricia Eugenia; Characterization of interactions of eggPC lipid structures with different biomolecules; Elsevier Ireland; Chemistry And Physics Of Lipids; 210; 1-2018; 60-69 0009-3084 CONICET Digital CONICET |
url |
http://hdl.handle.net/11336/97183 |
identifier_str_mv |
Corrales Chahar, F.; Díaz, S.B.; Ben Altabef, Aida; Gervasi, Claudio; Alvarez, Patricia Eugenia; Characterization of interactions of eggPC lipid structures with different biomolecules; Elsevier Ireland; Chemistry And Physics Of Lipids; 210; 1-2018; 60-69 0009-3084 CONICET Digital CONICET |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.chemphyslip.2017.11.013 info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0009308417302517?via%3Dihub |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
dc.format.none.fl_str_mv |
application/pdf application/pdf application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
Elsevier Ireland |
publisher.none.fl_str_mv |
Elsevier Ireland |
dc.source.none.fl_str_mv |
reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas |
reponame_str |
CONICET Digital (CONICET) |
collection |
CONICET Digital (CONICET) |
instname_str |
Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.name.fl_str_mv |
CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.mail.fl_str_mv |
dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
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1846781673014820864 |
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12.982451 |