Characterization of interactions of eggPC lipid structures with different biomolecules

Autores
Corrales Chahar, F.; Díaz, S.B.; Ben Altabef, Aida; Gervasi, Claudio; Alvarez, Patricia Eugenia
Año de publicación
2018
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
In this paper we study the interactions of two biomolecules (ascorbic acid and Annonacin) with a bilayer lipid membrane. Egg yolk phosphatidylcholine (eggPC) liposomes (in crystalline liquid state) were prepared in solutions of ascorbic acid (AA) at different concentration levels. On the other hand, liposomes were doped with Annonacin (Ann), a mono-tetrahydrofuran acetogenin (ACG), which is an effective citotoxic substance. While AA pharmacologic effect and action mechanisms are widely known, those of Ann's are only very recently being studied. Both Fourier Transformed Infrared (FTIR) and Raman spectroscopic techniques were used to study the participation of the main functional groups of the lipid bilayer involved in the membrane-solution interaction. The obtained spectra were comparatively analyzed, studying the spectral bands corresponding to both the hydrophobic and the hydrophilic regions in the lipid bilayer. Electrochemical experiments namely; impedance spectroscopy (EIS) and cyclic voltamperometry (CV) were used as the main characterization techniques to analyse stability and structural changes of a model system of supported EggPC bilayer in connection with its interactions with AA and Ann. At high molar ratios of AA, there is dehydration in both populations of the carbonyl group of the polar head of the lipid. On the other hand, Ann promotes the formation of hydrogen bonds with the carbonyl groups. No interaction between AA and phosphate groups is observed at low and intermediate molar ratios. Ann is expected to be able to induce the dehydration of the phosphate groups without the subsequent formation of H bonds with them. According to the electrochemical analysis, the interaction of AA with the supported lipid membrane does not alter its dielectric properties. This fact can be related to the conservation of structured water of the phosphate groups in the polar heads of the lipid. On the other hand, the incorporation of Ann into the lipid membrane generates an increase in the number of defects while changes the dielectric constant. This, in turn, can be associated with the induced dehydration of the phosphate groups.
Fil: Corrales Chahar, F.. Universidad Nacional de Tucumán; Argentina
Fil: Díaz, S.B.. Universidad Nacional de Tucumán; Argentina
Fil: Ben Altabef, Aida. Universidad Nacional de Tucumán; Argentina. Instituto de Química del Noroeste Argentino (inquinoa)-conicet-tucumán; Argentina
Fil: Gervasi, Claudio. Facultad de Ingeniería, Universidad Nacional de la Plata; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas; Argentina
Fil: Alvarez, Patricia Eugenia. Universidad Nacional de Tucumán; Argentina. Instituto de Química del Noroeste Argentino (inquinoa)-conicet-tucumán; Argentina
Materia
ANNONACEOUS ACETOGENINS
ASCORBIC ACID
EIS VOLTAMPEROMETRY
FTIR
RAMAN SPECTROSCOPY
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/97183
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/97183
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Characterization of interactions of eggPC lipid structures with different biomoleculesCorrales Chahar, F.Díaz, S.B.Ben Altabef, AidaGervasi, ClaudioAlvarez, Patricia EugeniaANNONACEOUS ACETOGENINSASCORBIC ACIDEIS VOLTAMPEROMETRYFTIRRAMAN SPECTROSCOPYhttps://purl.org/becyt/ford/1.4https://purl.org/becyt/ford/1In this paper we study the interactions of two biomolecules (ascorbic acid and Annonacin) with a bilayer lipid membrane. Egg yolk phosphatidylcholine (eggPC) liposomes (in crystalline liquid state) were prepared in solutions of ascorbic acid (AA) at different concentration levels. On the other hand, liposomes were doped with Annonacin (Ann), a mono-tetrahydrofuran acetogenin (ACG), which is an effective citotoxic substance. While AA pharmacologic effect and action mechanisms are widely known, those of Ann's are only very recently being studied. Both Fourier Transformed Infrared (FTIR) and Raman spectroscopic techniques were used to study the participation of the main functional groups of the lipid bilayer involved in the membrane-solution interaction. The obtained spectra were comparatively analyzed, studying the spectral bands corresponding to both the hydrophobic and the hydrophilic regions in the lipid bilayer. Electrochemical experiments namely; impedance spectroscopy (EIS) and cyclic voltamperometry (CV) were used as the main characterization techniques to analyse stability and structural changes of a model system of supported EggPC bilayer in connection with its interactions with AA and Ann. At high molar ratios of AA, there is dehydration in both populations of the carbonyl group of the polar head of the lipid. On the other hand, Ann promotes the formation of hydrogen bonds with the carbonyl groups. No interaction between AA and phosphate groups is observed at low and intermediate molar ratios. Ann is expected to be able to induce the dehydration of the phosphate groups without the subsequent formation of H bonds with them. According to the electrochemical analysis, the interaction of AA with the supported lipid membrane does not alter its dielectric properties. This fact can be related to the conservation of structured water of the phosphate groups in the polar heads of the lipid. On the other hand, the incorporation of Ann into the lipid membrane generates an increase in the number of defects while changes the dielectric constant. This, in turn, can be associated with the induced dehydration of the phosphate groups.Fil: Corrales Chahar, F.. Universidad Nacional de Tucumán; ArgentinaFil: Díaz, S.B.. Universidad Nacional de Tucumán; ArgentinaFil: Ben Altabef, Aida. Universidad Nacional de Tucumán; Argentina. Instituto de Química del Noroeste Argentino (inquinoa)-conicet-tucumán; ArgentinaFil: Gervasi, Claudio. Facultad de Ingeniería, Universidad Nacional de la Plata; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas; ArgentinaFil: Alvarez, Patricia Eugenia. Universidad Nacional de Tucumán; Argentina. Instituto de Química del Noroeste Argentino (inquinoa)-conicet-tucumán; ArgentinaElsevier Ireland2018-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/97183Corrales Chahar, F.; Díaz, S.B.; Ben Altabef, Aida; Gervasi, Claudio; Alvarez, Patricia Eugenia; Characterization of interactions of eggPC lipid structures with different biomolecules; Elsevier Ireland; Chemistry And Physics Of Lipids; 210; 1-2018; 60-690009-3084CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.chemphyslip.2017.11.013info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0009308417302517?via%3Dihubinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-22T11:19:29Zoai:ri.conicet.gov.ar:11336/97183instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-22 11:19:29.808CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Characterization of interactions of eggPC lipid structures with different biomolecules
title Characterization of interactions of eggPC lipid structures with different biomolecules
spellingShingle Characterization of interactions of eggPC lipid structures with different biomolecules
Corrales Chahar, F.
ANNONACEOUS ACETOGENINS
ASCORBIC ACID
EIS VOLTAMPEROMETRY
FTIR
RAMAN SPECTROSCOPY
title_short Characterization of interactions of eggPC lipid structures with different biomolecules
title_full Characterization of interactions of eggPC lipid structures with different biomolecules
title_fullStr Characterization of interactions of eggPC lipid structures with different biomolecules
title_full_unstemmed Characterization of interactions of eggPC lipid structures with different biomolecules
title_sort Characterization of interactions of eggPC lipid structures with different biomolecules
dc.creator.none.fl_str_mv Corrales Chahar, F.
Díaz, S.B.
Ben Altabef, Aida
Gervasi, Claudio
Alvarez, Patricia Eugenia
author Corrales Chahar, F.
author_facet Corrales Chahar, F.
Díaz, S.B.
Ben Altabef, Aida
Gervasi, Claudio
Alvarez, Patricia Eugenia
author_role author
author2 Díaz, S.B.
Ben Altabef, Aida
Gervasi, Claudio
Alvarez, Patricia Eugenia
author2_role author
author
author
author
dc.subject.none.fl_str_mv ANNONACEOUS ACETOGENINS
ASCORBIC ACID
EIS VOLTAMPEROMETRY
FTIR
RAMAN SPECTROSCOPY
topic ANNONACEOUS ACETOGENINS
ASCORBIC ACID
EIS VOLTAMPEROMETRY
FTIR
RAMAN SPECTROSCOPY
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.4
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv In this paper we study the interactions of two biomolecules (ascorbic acid and Annonacin) with a bilayer lipid membrane. Egg yolk phosphatidylcholine (eggPC) liposomes (in crystalline liquid state) were prepared in solutions of ascorbic acid (AA) at different concentration levels. On the other hand, liposomes were doped with Annonacin (Ann), a mono-tetrahydrofuran acetogenin (ACG), which is an effective citotoxic substance. While AA pharmacologic effect and action mechanisms are widely known, those of Ann's are only very recently being studied. Both Fourier Transformed Infrared (FTIR) and Raman spectroscopic techniques were used to study the participation of the main functional groups of the lipid bilayer involved in the membrane-solution interaction. The obtained spectra were comparatively analyzed, studying the spectral bands corresponding to both the hydrophobic and the hydrophilic regions in the lipid bilayer. Electrochemical experiments namely; impedance spectroscopy (EIS) and cyclic voltamperometry (CV) were used as the main characterization techniques to analyse stability and structural changes of a model system of supported EggPC bilayer in connection with its interactions with AA and Ann. At high molar ratios of AA, there is dehydration in both populations of the carbonyl group of the polar head of the lipid. On the other hand, Ann promotes the formation of hydrogen bonds with the carbonyl groups. No interaction between AA and phosphate groups is observed at low and intermediate molar ratios. Ann is expected to be able to induce the dehydration of the phosphate groups without the subsequent formation of H bonds with them. According to the electrochemical analysis, the interaction of AA with the supported lipid membrane does not alter its dielectric properties. This fact can be related to the conservation of structured water of the phosphate groups in the polar heads of the lipid. On the other hand, the incorporation of Ann into the lipid membrane generates an increase in the number of defects while changes the dielectric constant. This, in turn, can be associated with the induced dehydration of the phosphate groups.
Fil: Corrales Chahar, F.. Universidad Nacional de Tucumán; Argentina
Fil: Díaz, S.B.. Universidad Nacional de Tucumán; Argentina
Fil: Ben Altabef, Aida. Universidad Nacional de Tucumán; Argentina. Instituto de Química del Noroeste Argentino (inquinoa)-conicet-tucumán; Argentina
Fil: Gervasi, Claudio. Facultad de Ingeniería, Universidad Nacional de la Plata; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas; Argentina
Fil: Alvarez, Patricia Eugenia. Universidad Nacional de Tucumán; Argentina. Instituto de Química del Noroeste Argentino (inquinoa)-conicet-tucumán; Argentina
description In this paper we study the interactions of two biomolecules (ascorbic acid and Annonacin) with a bilayer lipid membrane. Egg yolk phosphatidylcholine (eggPC) liposomes (in crystalline liquid state) were prepared in solutions of ascorbic acid (AA) at different concentration levels. On the other hand, liposomes were doped with Annonacin (Ann), a mono-tetrahydrofuran acetogenin (ACG), which is an effective citotoxic substance. While AA pharmacologic effect and action mechanisms are widely known, those of Ann's are only very recently being studied. Both Fourier Transformed Infrared (FTIR) and Raman spectroscopic techniques were used to study the participation of the main functional groups of the lipid bilayer involved in the membrane-solution interaction. The obtained spectra were comparatively analyzed, studying the spectral bands corresponding to both the hydrophobic and the hydrophilic regions in the lipid bilayer. Electrochemical experiments namely; impedance spectroscopy (EIS) and cyclic voltamperometry (CV) were used as the main characterization techniques to analyse stability and structural changes of a model system of supported EggPC bilayer in connection with its interactions with AA and Ann. At high molar ratios of AA, there is dehydration in both populations of the carbonyl group of the polar head of the lipid. On the other hand, Ann promotes the formation of hydrogen bonds with the carbonyl groups. No interaction between AA and phosphate groups is observed at low and intermediate molar ratios. Ann is expected to be able to induce the dehydration of the phosphate groups without the subsequent formation of H bonds with them. According to the electrochemical analysis, the interaction of AA with the supported lipid membrane does not alter its dielectric properties. This fact can be related to the conservation of structured water of the phosphate groups in the polar heads of the lipid. On the other hand, the incorporation of Ann into the lipid membrane generates an increase in the number of defects while changes the dielectric constant. This, in turn, can be associated with the induced dehydration of the phosphate groups.
publishDate 2018
dc.date.none.fl_str_mv 2018-01
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/97183
Corrales Chahar, F.; Díaz, S.B.; Ben Altabef, Aida; Gervasi, Claudio; Alvarez, Patricia Eugenia; Characterization of interactions of eggPC lipid structures with different biomolecules; Elsevier Ireland; Chemistry And Physics Of Lipids; 210; 1-2018; 60-69
0009-3084
CONICET Digital
CONICET
url http://hdl.handle.net/11336/97183
identifier_str_mv Corrales Chahar, F.; Díaz, S.B.; Ben Altabef, Aida; Gervasi, Claudio; Alvarez, Patricia Eugenia; Characterization of interactions of eggPC lipid structures with different biomolecules; Elsevier Ireland; Chemistry And Physics Of Lipids; 210; 1-2018; 60-69
0009-3084
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1016/j.chemphyslip.2017.11.013
info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0009308417302517?via%3Dihub
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Ireland
publisher.none.fl_str_mv Elsevier Ireland
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 12.982451

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