Control of cell adhesion and migration by podocalyxin. Implication of Rac1 and Cdc42

Autores
Fernández, Darío; Horrillo, Angélica; Alquezar, Carolina; Gonzalez-Manchon, Consuelo; Parrilla, Roberto; Ayuso, Matilde S.
Año de publicación
2013
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Podocalyxin (PODXL) is a type I membrane sialomucin, originally described in the epithelial cells (podocytes) of kidney glomeruli. PODXL is also found in extra-renal tissues and in certain aggressive tumors, but its precise pathophysiological role is unknown. Expression of PODXL in CHO cells enhances their adhesive, migratory and cell?cell interactive properties in a selection and integrin-dependent manner. We aimed at defining the PODXL domains responsible for those cell responses. For this purpose we have analyzed the cell adhesion/migration responses to deletion mutants of human PODXL, and the correlation with the activities of Rac1 and Cdc42 GTPases. The results obtained indicate that integrity of the PODXL ectodomain is essential for enhancing cell adhesion but not migration, while the integrity of the cytoplasmic domain is required for both adhesion and migration. Deletion of the carboxy-terminal DTHL domain (PODXL-ΔDTHL) limited only cell adhesion. The activities of Rac1 and Cdc42 GTPases parallel the PODXL-induced variations in cell adhesion and migration. Moreover, silencing the rac1 gene virtually abolished the effect of PODXL in enhancing cell adhesion.
Fil: Fernández, Darío. Consejo Nacional de Investigaciones Científicas y Tecnicas. Centro Cientifico Tecnologico Mendoza. Instituto Histologia y Embriologia de Mendoza "Dr. M. Burgos"; Argentina. Consejo Superior de Investigaciones Cientificas. Centro de Investigaciones Biologicas. Departamento de Medicina Celular y Molecular; España
Fil: Horrillo, Angélica. CIBER de enfermedades raras; España
Fil: Alquezar, Carolina. Consejo Superior de Investigaciones Cientificas. Centro de Investigaciones Biologicas. Departamento de Medicina Celular y Molecular; España
Fil: Gonzalez-Manchon, Consuelo. Consejo Superior de Investigaciones Cientificas. Centro de Investigaciones Biologicas. Departamento de Medicina Celular y Molecula; España. CIBER de enfermedades raras; España
Fil: Parrilla, Roberto. Consejo Superior de Investigaciones Cientificas. Centro de Investigaciones Biologicas. Departamento de Medicina Celular y Molecular; España. CIBER de enfermedades raras; España
Fil: Ayuso, Matilde S.. Consejo Superior de Investigaciones Cientificas. Centro de Investigaciones Biologicas. Departamento de Medicina Celular y Molecular; España. CIBER de enfermedades raras; España
Materia
Cell Adhesion
Cell Migration
Podocalyxin
Rac-1 And Cdc-42
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/14536
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/14536
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Control of cell adhesion and migration by podocalyxin. Implication of Rac1 and Cdc42Fernández, DaríoHorrillo, AngélicaAlquezar, CarolinaGonzalez-Manchon, ConsueloParrilla, RobertoAyuso, Matilde S.Cell AdhesionCell MigrationPodocalyxinRac-1 And Cdc-42https://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Podocalyxin (PODXL) is a type I membrane sialomucin, originally described in the epithelial cells (podocytes) of kidney glomeruli. PODXL is also found in extra-renal tissues and in certain aggressive tumors, but its precise pathophysiological role is unknown. Expression of PODXL in CHO cells enhances their adhesive, migratory and cell?cell interactive properties in a selection and integrin-dependent manner. We aimed at defining the PODXL domains responsible for those cell responses. For this purpose we have analyzed the cell adhesion/migration responses to deletion mutants of human PODXL, and the correlation with the activities of Rac1 and Cdc42 GTPases. The results obtained indicate that integrity of the PODXL ectodomain is essential for enhancing cell adhesion but not migration, while the integrity of the cytoplasmic domain is required for both adhesion and migration. Deletion of the carboxy-terminal DTHL domain (PODXL-ΔDTHL) limited only cell adhesion. The activities of Rac1 and Cdc42 GTPases parallel the PODXL-induced variations in cell adhesion and migration. Moreover, silencing the rac1 gene virtually abolished the effect of PODXL in enhancing cell adhesion.Fil: Fernández, Darío. Consejo Nacional de Investigaciones Científicas y Tecnicas. Centro Cientifico Tecnologico Mendoza. Instituto Histologia y Embriologia de Mendoza "Dr. M. Burgos"; Argentina. Consejo Superior de Investigaciones Cientificas. Centro de Investigaciones Biologicas. Departamento de Medicina Celular y Molecular; EspañaFil: Horrillo, Angélica. CIBER de enfermedades raras; EspañaFil: Alquezar, Carolina. Consejo Superior de Investigaciones Cientificas. Centro de Investigaciones Biologicas. Departamento de Medicina Celular y Molecular; EspañaFil: Gonzalez-Manchon, Consuelo. Consejo Superior de Investigaciones Cientificas. Centro de Investigaciones Biologicas. Departamento de Medicina Celular y Molecula; España. CIBER de enfermedades raras; EspañaFil: Parrilla, Roberto. Consejo Superior de Investigaciones Cientificas. Centro de Investigaciones Biologicas. Departamento de Medicina Celular y Molecular; España. CIBER de enfermedades raras; EspañaFil: Ayuso, Matilde S.. Consejo Superior de Investigaciones Cientificas. Centro de Investigaciones Biologicas. Departamento de Medicina Celular y Molecular; España. CIBER de enfermedades raras; EspañaElsevier2013-02-08info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/14536Fernández, Darío; Horrillo, Angélica; Alquezar, Carolina; Gonzalez-Manchon, Consuelo; Parrilla, Roberto; et al.; Control of cell adhesion and migration by podocalyxin. Implication of Rac1 and Cdc42; Elsevier; Biochemical And Biophysical Research Communications; 432; 2; 8-2-2013; 302-3070006-291Xenginfo:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0006291X13002131info:eu-repo/semantics/altIdentifier/url/http://dx.doi.org/10.1016/j.bbrc.2013.01.112info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:59:52Zoai:ri.conicet.gov.ar:11336/14536instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:59:52.384CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Control of cell adhesion and migration by podocalyxin. Implication of Rac1 and Cdc42
title Control of cell adhesion and migration by podocalyxin. Implication of Rac1 and Cdc42
spellingShingle Control of cell adhesion and migration by podocalyxin. Implication of Rac1 and Cdc42
Fernández, Darío
Cell Adhesion
Cell Migration
Podocalyxin
Rac-1 And Cdc-42
title_short Control of cell adhesion and migration by podocalyxin. Implication of Rac1 and Cdc42
title_full Control of cell adhesion and migration by podocalyxin. Implication of Rac1 and Cdc42
title_fullStr Control of cell adhesion and migration by podocalyxin. Implication of Rac1 and Cdc42
title_full_unstemmed Control of cell adhesion and migration by podocalyxin. Implication of Rac1 and Cdc42
title_sort Control of cell adhesion and migration by podocalyxin. Implication of Rac1 and Cdc42
dc.creator.none.fl_str_mv Fernández, Darío
Horrillo, Angélica
Alquezar, Carolina
Gonzalez-Manchon, Consuelo
Parrilla, Roberto
Ayuso, Matilde S.
author Fernández, Darío
author_facet Fernández, Darío
Horrillo, Angélica
Alquezar, Carolina
Gonzalez-Manchon, Consuelo
Parrilla, Roberto
Ayuso, Matilde S.
author_role author
author2 Horrillo, Angélica
Alquezar, Carolina
Gonzalez-Manchon, Consuelo
Parrilla, Roberto
Ayuso, Matilde S.
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Cell Adhesion
Cell Migration
Podocalyxin
Rac-1 And Cdc-42
topic Cell Adhesion
Cell Migration
Podocalyxin
Rac-1 And Cdc-42
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Podocalyxin (PODXL) is a type I membrane sialomucin, originally described in the epithelial cells (podocytes) of kidney glomeruli. PODXL is also found in extra-renal tissues and in certain aggressive tumors, but its precise pathophysiological role is unknown. Expression of PODXL in CHO cells enhances their adhesive, migratory and cell?cell interactive properties in a selection and integrin-dependent manner. We aimed at defining the PODXL domains responsible for those cell responses. For this purpose we have analyzed the cell adhesion/migration responses to deletion mutants of human PODXL, and the correlation with the activities of Rac1 and Cdc42 GTPases. The results obtained indicate that integrity of the PODXL ectodomain is essential for enhancing cell adhesion but not migration, while the integrity of the cytoplasmic domain is required for both adhesion and migration. Deletion of the carboxy-terminal DTHL domain (PODXL-ΔDTHL) limited only cell adhesion. The activities of Rac1 and Cdc42 GTPases parallel the PODXL-induced variations in cell adhesion and migration. Moreover, silencing the rac1 gene virtually abolished the effect of PODXL in enhancing cell adhesion.
Fil: Fernández, Darío. Consejo Nacional de Investigaciones Científicas y Tecnicas. Centro Cientifico Tecnologico Mendoza. Instituto Histologia y Embriologia de Mendoza "Dr. M. Burgos"; Argentina. Consejo Superior de Investigaciones Cientificas. Centro de Investigaciones Biologicas. Departamento de Medicina Celular y Molecular; España
Fil: Horrillo, Angélica. CIBER de enfermedades raras; España
Fil: Alquezar, Carolina. Consejo Superior de Investigaciones Cientificas. Centro de Investigaciones Biologicas. Departamento de Medicina Celular y Molecular; España
Fil: Gonzalez-Manchon, Consuelo. Consejo Superior de Investigaciones Cientificas. Centro de Investigaciones Biologicas. Departamento de Medicina Celular y Molecula; España. CIBER de enfermedades raras; España
Fil: Parrilla, Roberto. Consejo Superior de Investigaciones Cientificas. Centro de Investigaciones Biologicas. Departamento de Medicina Celular y Molecular; España. CIBER de enfermedades raras; España
Fil: Ayuso, Matilde S.. Consejo Superior de Investigaciones Cientificas. Centro de Investigaciones Biologicas. Departamento de Medicina Celular y Molecular; España. CIBER de enfermedades raras; España
description Podocalyxin (PODXL) is a type I membrane sialomucin, originally described in the epithelial cells (podocytes) of kidney glomeruli. PODXL is also found in extra-renal tissues and in certain aggressive tumors, but its precise pathophysiological role is unknown. Expression of PODXL in CHO cells enhances their adhesive, migratory and cell?cell interactive properties in a selection and integrin-dependent manner. We aimed at defining the PODXL domains responsible for those cell responses. For this purpose we have analyzed the cell adhesion/migration responses to deletion mutants of human PODXL, and the correlation with the activities of Rac1 and Cdc42 GTPases. The results obtained indicate that integrity of the PODXL ectodomain is essential for enhancing cell adhesion but not migration, while the integrity of the cytoplasmic domain is required for both adhesion and migration. Deletion of the carboxy-terminal DTHL domain (PODXL-ΔDTHL) limited only cell adhesion. The activities of Rac1 and Cdc42 GTPases parallel the PODXL-induced variations in cell adhesion and migration. Moreover, silencing the rac1 gene virtually abolished the effect of PODXL in enhancing cell adhesion.
publishDate 2013
dc.date.none.fl_str_mv 2013-02-08
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/14536
Fernández, Darío; Horrillo, Angélica; Alquezar, Carolina; Gonzalez-Manchon, Consuelo; Parrilla, Roberto; et al.; Control of cell adhesion and migration by podocalyxin. Implication of Rac1 and Cdc42; Elsevier; Biochemical And Biophysical Research Communications; 432; 2; 8-2-2013; 302-307
0006-291X
url http://hdl.handle.net/11336/14536
identifier_str_mv Fernández, Darío; Horrillo, Angélica; Alquezar, Carolina; Gonzalez-Manchon, Consuelo; Parrilla, Roberto; et al.; Control of cell adhesion and migration by podocalyxin. Implication of Rac1 and Cdc42; Elsevier; Biochemical And Biophysical Research Communications; 432; 2; 8-2-2013; 302-307
0006-291X
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0006291X13002131
info:eu-repo/semantics/altIdentifier/url/http://dx.doi.org/10.1016/j.bbrc.2013.01.112
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1842269606300876800
score 13.13397

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